Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Effects of Lyophyllum shimeji Inoculation on the Mycorrhizal Formation and Seedling Growth of Lespedeza cyrtobortya

땅찌만가닥버섯균(菌)의 접종(接種)이 참싸리 묘목(苗木)의 균근형성(菌根形成)과 생장(生長)에 미치는 영향(影響)

  • Lee, Sang Yong (Division of Forest Resources, College of Forest Sciences, Kangwon National University) ;
  • Jung, Joo Hae (Division of Forest Resources, College of Forest Sciences, Kangwon National University) ;
  • Lee, Jong Kyu (Division of Forest Resources, College of Forest Sciences, Kangwon National University)
  • 이상용 (강원대학교 산림과학대학 산림자원학부) ;
  • 정주해 (강원대학교 산림과학대학 산림자원학부) ;
  • 이종규 (강원대학교 산림과학대학 산림자원학부)
  • Received : 2005.02.28
  • Accepted : 2005.04.28
  • Published : 2005.06.30
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Abstract

For the application of ectomycorrhizal seedlings on damaged slope lands, studies on cultural characteristics of Lyophyllum shimeji and ectomycorrhizal associations of Lespedeza cyrtobotrya seedlings were carried out by artificial inoculation of L. shimeji. Mycelial growth of L. shimeji was best on MP (1% malt extract, 0.1% peptone, 1% glucose and 1.5% agar) medium. An optimum temperature and pH for the mycelial growth were $25^{\circ}C$ and pH6, respectively. Mycorrhizal root of L. cyrtobotrya seedlings inoculated with L. shimeji showed characteristics of ectomycorrhizas with Hartig net. Growth rate of the mycorrhizal seedlings's roots was higher than that of non-mycorrhizal seedlings. When the mycorrhizal seedlings were transplanted in slope land, survival rate and dry weight were 62% and 850 mg/seedling, respectively. On the other hand, survival rate and dry weight of non-mycorrhizal seedlings were 11% and 430 mg/seedling, respectively.

훼손된 비탈면의 조림에 균근균의 이용가능성을 타진하기 위하여, 땅찌만가닥버섯(Lyophyllum shimeji)의 배양학적 특성 및 인공접종에 의한 참싸리(Lespedeza cyrtobotrya)에서의 균근 형성 특성을 조사하였다. L. shimeji는 MP (1% malt extract, 0.1% peptone, 1% glucose, 1.5% agar)배지에서 균사 생장이 가장 왕성하였으며, 균사 최적 배양 온도 및 pH는 각각 $25^{\circ}C$ 및 pH 6 이었다. 인공접종에 의한 참싸리에서의 균근 형성 특성을 조사한 결과, L. shimeji는 Hartig net을 형성하는 외생균근의 특성을 나타내었으며, 균근 접종 실생 묘목 뿌리의 생장율은 대조구에 비하여 우수하였다. 균근 접종 실생 묘목을 비탈면에 이식하였을 때의 생존율 및 건중량은 각각 62% 및 850 mg/본 이었으나, 균근을 접종하지 않은 실생 묘목의 생존율 및 건중량은 각각 11% 및 430 mg/본 이었다.

Keywords

References

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