• Title/Summary/Keyword: Korean lespedeza

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Antioxidant Activity of Different Parts of Lespedeza bicolor and Isolation of Antioxidant Compound (싸리나무(Lespedeza bicolor) 부위별 추출물의 항산화 활성 및 항산화물질 분리)

  • Lee, Jae-Hak;Jhoo, Jin-Woo
    • Korean Journal of Food Science and Technology
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    • v.44 no.6
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    • pp.763-771
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    • 2012
  • In this study, total antioxidant properties of extracts from different parts of Lespedeza bicolor were determined using techniques of measuring 1,1-diphenyl-2-picryl hydrazyl/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity and total phenolic contents. The total antioxidant activities of leaf, stem and root extracts from various solvents (water, 50, 70, 100% ethanol, and hot-water) indicated that 50 and 70% ethanol extracts have high radical scavenging activities and phenolic contents. A systematic approach was used to determine the total antioxidant activity of different solvent fractions of the Lespedeza bicolor extracts, partitioning with chloroform, ethyl acetate, n-butanol, and water, and the ethyl acetate fraction was found to have the strongest antioxidant activity. Antioxidant assay-guided isolation was carried out to isolate potential antioxidant compounds. The ethyl acetate fraction of the leaf extract was subjected to silica gel, LH-20 and RP-18 column chromatography successively, and afforded compound 1, which was identified as eriodictyol by NMR and MS analysis, after which its antioxidant activity was determined.

Breaking Physical Dormancy with Sulfuric Acid in Seeds of Lespedeza tomentosa (Thunb.) Siebold ex Maxim (황산처리를 이용한 개싸리 종자의 물리적 휴면 타파)

  • Rhie, Yong Ha;Choi, Han;Lee, Su Gwang;Lee, Jeong Ho;Lee, Ki Cheol
    • Korean Journal of Plant Resources
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    • v.29 no.1
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    • pp.136-142
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    • 2016
  • Lespedeza species are mainly used for wildlife food and cover and for erosion control. The germination of these species can be enhanced after a fire occurrence in forest, which is known as fire-activated seeds to germinate. While the heat treatment could break seed dormancy of Lespedeza, its germination rate was quite low. We investigated that chemical scarification could promote germination of L. tomentosa. Seeds were soaked in 100% sulfuric acid (H2SO4) for 0, 1, 3, 6, 12, 24, 48, 96, 192, and 384 min, and then washed in distilled water for 24 h. Very few seeds were germinated in control (H2SO4 for 0 min). More than 90% of seeds were germinated in H2SO4 for 24, 48, and 92 min. However, some damage was observed in roots and cotyledons of seedling dipped in H2SO4 for a long time. To search the optimal soaking time in H2SO4 without defects, seeds scarified in H2SO4 for 30, 60, 90, 120, 150, 180, and 300 min were sown the commercial soil medium. Seeds treated with H2SO4 for 90 min and 150 min emerged by about 92% and 84%, respectively. Therefore, H2SO4 treatment could break the seed dormancy of Lespedeza species, and especially in case of L. tomentosa the optimal treatment time in sulfuric acid was one to two hours. Germination of L. tomentosa began promptly following the scarification and was completed within about one month, indicating that seeds has no physiological dormancy, just has physical dormancy.

Inhibition of Cell Proliferation and Induction of Apoptosis by Ethanolic Extract of Lespedeza cuneata G. Don in Human Colorectal Cancer HT-29 cells (야관문의 에탄올 추출물에 의한 대장암세포의 성장억제 및 세포사멸유도)

  • Zhao, Qian;Kim, Yeah-Un;Han, In-Hwa;Yun, Jung-Mi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.6
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    • pp.911-917
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    • 2016
  • Lespedeza cuneata G. Don is an edible perennial herb used in traditional Korean medicine. We investigated the anti-proliferative properties and mechanism of L. cuneata extract. The ethanolic extract of L. cuneata dose-and time-dependently inhibited human colorectal cancer cell proliferation. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to test the effect of the extract on proliferation of HT-29 colorectal cancer cells. The extract inhibited HT-29 cell proliferation with an $IC_{50}$ value of $554.26{\pm}8.81{\mu}g/mL$. L. cuneata extract suppressed production of pro-inflammatory cytokines interleukin-6 and tumor necrosis $factor-{\alpha}$. Apoptosis was evaluated by analysis of DNA fragmentation, poly(ADP-ribose) polymerase cleavage, caspase-3 activity, and protein expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2). Our results demonstrated that the extract induced DNA fragmentation and characteristic morphological changes associated with apoptosis in HT-29 colorectal cancer cells. The extract also time- and dose-dependently up-regulated expression of the Bax and down-regulated expression of the Bcl-2. Furthermore, the extract dose- and time-dependently enhanced caspase-3 activity. Our findings provide evidence that L. cuneata extract may mediate its anti-proliferative effect via modulation of apoptosis.

Development of Revegetation Measures using Boring Technique in Rock Slopes - Focus on Lespedeza cyrtobotrya - (암반비탈면에 있어서 천공기법에 의한 녹화공법의 개발 - 참싸리를 중심으로 -)

  • Ma, Ho-Seop;Kang, Won-Seok;Park, Jin-Won
    • Journal of Korean Society of Forest Science
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    • v.100 no.4
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    • pp.558-564
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    • 2011
  • This study was conducted to evaluate the effects of early revegetation by analyzing the characteristics of germination and growth of Lespedeza cyrtobotrya using boring technique in rock slopes. After making up a growing basis of approximately 20 cm depth and 10 cm diameter by using a boring machine, the surface of rock slopes was planted with vegetation-plant. The number of germinating populations by soil media was 23 in H.s, 22 in T.s, 12 in M.s, and 1 in M.g.s. The germination rate (%) by soil media was 19.2% in H.s, 18.3% in T.s, 10.0% in M.s and 0.8% in M.g.s. In monthly changes of growth rate, the aspect was northwest direction, the soil media was M.s, and the treatment was microorganism plot. The main factors affecting survivorship and growth of population were soil media and treatment plot. The interaction between each factor had a good effects in bearing ${\times}$ soil media, bearing ${\times}$ treatment plot, soil media ${\times}$ treatment plot. but, it is recommended that the mulching of vegetation plant is highly needed to help the germination of seed and growth of vegetation because of loss of seed and soil media occurred due to rainfall. Therefore, The result suggests that the revegetation technique using boring in rock slope was very efficient in respect of the early revegetation and the landscape.

The effects of aqueous extracts of plant roots on germination of seeds and growth of seedings (식물근의 추출물질이 종자발아 및 유식물의 생장에 미치는 영향)

  • Chan-Ho Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.1-23
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    • 1968
  • This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

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Effect of a Mixed Extract of Fenugreek Seeds and Lespedeza cuneata on Testosterone Deficiency Syndrome (호로파와 야관문 복합추출물의 남성갱년기 개선에 관한 연구)

  • Lee, Kyeong Soo;Lee, Eun Kyung;Kim, Shin Yeon;Kim, Tae Hwan;Kim, Hyun Pyo
    • Korean Journal of Food Science and Technology
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    • v.47 no.4
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    • pp.492-498
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    • 2015
  • Testosterone deficiency syndrome (TDS) is normally observed in elderly men and is a clinical and biochemical syndrome, characterized by a decline in plasmic testosterone levels resulting in a significant decrease in quality of life. The aim of this study was to evaluate the effect of a mixed extract of fenugreek seeds and Lespedeza cuneata (YHM) on TDS. Rats were divided into three groups: the negative control, YHM-40 (40 mg/kg), and YHM-80 (80 mg/kg) groups. After 4 weeks of YMH administration, an increase was observed in the plasmic testosterone levels, vastus lateralis muscular strength, forced swimming time, total sperm counts, and motile sperm counts in YHM-40 and YHM-80 groups compared to the negative control group. Moreover, sex hormone binding globulin, the epididymal fat pad, total plasmic cholesterol, and triglyceride levels were significantly decreased in the YHM-fed groups. However, prostate specific antigen, aspartate aminotransferase, and alanine aminotransferase levels did not differ among the groups. These results suggest that YHM may enhance testosterone levels in elderly men and alleviate TDS without common side effects.

Native Plants Selection for Ecological Replantation and Vascular Plants in Forest Road Slope - In Case Study on Forest Road of Gyeongsangnam-do and Jeollanam-do - (임도사면의 생태적 녹화를 위한 자생식물 선정 및 관속식물상 - 경상남도와 전라남도 임도를 중심으로 -)

  • 이미정;이준우;전권석;지윤의;김명준;김종윤;송호경
    • Korean Journal of Environment and Ecology
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    • v.17 no.3
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    • pp.201-209
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    • 2003
  • This study was carried out to select proper species fur early stage replantation in forest road cut-slope. From one to fifteen years elapsed forest roads in four regional areas of Gyeongsangnam-do and Jeollanam-do, sample plots were selected, and their vegetations and environmental factors were investigated. Invaded species and plant coverage have increased with elapsed years. The occurrence rate of herbs have decreased, while the occurrence of trees have increased with elapsed years. The ordination analysis showed that distribution of species was influenced by the elapsed year, the slope angle of the cut-slope, elevation, and the direction angle of the cut-slope. Dactylis glomerata, Poa pratensis, Amorpha fruticosa, Lespedeza sp., Artemisia princeps var. orientalis, Duchesnea chrysantha, Miscanthus sinensis, Pueraria thunbergiana, Zanthoxylum schinifolium, Rhus chinensis, and Weigela subsessilis were considered as proper species for replantation in forest road cut-slope, besides the species used already. Seventy seven family, 190 genus, 233 species, 38 variety, and 271 species in total were counted as invaded plant species in the survey zone. Naturalized plants were 18 species. Invaded species are, with high frequency first, Rubus crataegifolius, Artemisia princeps var. orientalis, Zanthoxylum schinifolium, Pueraria thunbergiana, Pinus densiflora, Lespedeza maximowiczii, Lysimachia clethroides, Lespedeza bicolor, Youngia denticulata, Miscanthus sinensis, and Erigeron annuus.

Polyphenol Contents and Physiological Activity of the Lespedeza bicolor Extracts (싸리 추출물의 폴리페놀 함량과 생리활성)

  • Lee, Yang-Suk;Joo, Eun-Young;Kim, Nam-Woo
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.616-622
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    • 2006
  • This study was intended to analyze the contents of polyphenol compounds and vitamin C, and the inhibitory activities of xanthine oxidase and tyrosinase to measure physiological efface of various extract of Lespedeza bicolor. The vitamin C content was $26.42{\pm}0.08mg/100g$. The content of phenolic compounds included in the flesh L bicolor was $1.14{\pm}0.02mg/g$. The ethanol extract by reflux method and water extracts by the microwave-assisted method showed the highest content as 154.95 mg/g and 145.17 mg/g, respectively. The xanthine oxidase inhibitory tate of water extracts was the highest value of 87.85% at the concentration of 1,000 ug/mL. All kinds of extracts showed the highest inhibitory activity for the xanthine oxidase at the concentration of 1,000 ug/mL and a decreasing pattern of the inhibitory race over the concentration of 1,000 ug/mL. The tyrosinase inhibitory rate was increased with an increment of the extract concentrations, and the activity of ethanol extracts was the highest as 55.22% at the concentration of 3,000 ug/mL.

The Cuttings of the Genus Lespedeza (싸리류(類)의 삽목시험(揷木試驗))

  • Han, Young Chang
    • Journal of Korean Society of Forest Science
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    • v.20 no.1
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    • pp.45-50
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    • 1973
  • This study was investigated the methods of vegetative propagation for new Lespedeza varieties. Experimental matterials were used 6 Lespedeza species; L. maximowiczii, L. cyrtobotrya, L. angustifolioides, L. bicolor, L. maximowiczii var. tomentella, and L. japonica var. intermedia. In April a year branches were cut 15-20cm length and 3-4mm thickness and planted in sand bed (depth 150cm) to be sterilized by the 0.1% solution of Uspulun. And then the cutting beds were irrigated and shaded. The results are as follows. 1. The rooting rates of the cuttings could be found so variable among varieties from 69% of L. maximowiczii var. tomentella to 50.3% of L. japonica var. intermedia. 2. The growth performance showed statistically significant difference at 1% level among varieties. L. maximowiczii was the best, while L. japonica var. intermedia showed the wrost growth performance of them. 3. The average number of roots with more than 5 cm length per stock was revealed statistically significant difference at the 1% level among varieties from the greatest number of 6.4 of L. maximowiczii var. tomentella to the smallest number of 2.3 of L. japonica var. intermedia. 4. Total average length of the roots per stock was statistically significant so different at 1% level from the longest 279.8cm of L. maximowiczii var. tomentella to the shortest 41.1cm of L. japonica var. intermedia. 5. The average ndules with more than 1 mm in diameter per stock was statistically significant so different at 1% level among varieties from the greatest number of 34.4 of L. maximowcizii to the smallest numqer of 4.6 of L. japonica var. intermedia.

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Protective Effects of Lespedeza bicolor Extract on B16/F10 Melanoma Cell Lines Damaged by Lead Acetate, Heavy Metal Compounds (중금속 화합물인 초산납으로 손상된 B16/F10 멜라닌세포주에 대한 싸리나무 추출물의 보호 효과)

  • Seo, Young-Mi
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.4
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    • pp.363-370
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    • 2021
  • This study was conducted to evaluate the dermal cytotoxicity of lead acetate (LA) and other heavy metal compounds, and the protective effect of Lespedeza bicolor (LB) extract on LA-induced cytotoxicity in cultured B16-/F10 melanoma cells. The study evaluated the antioxidative effects of LB due to its electron-donating ability (EDA), inhibitory effects on melanization and improving cell viability. LA significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was determined at 52.7 µM in the studied cultures. Based on the Borenfreund and Puerner's toxicity criteria, LA was estimated to be highly cytotoxic. LA-induced cytotoxicity and cell damage was reversed by the antioxidant activity of kaempferol (KAE), thereby remarkably improving cell viability. A study of the protective effects of the LB extract on LA-induced cytotoxicity showed that the LB extract remarkably increased cell viability in the LA-treated group, and also inhibited the EDA and the total amount of melanin. The above results suggest oxidative stress-mediated cytotoxicity of LA. In the study, LB extract effectively prevented LA-induced cytotoxicity via its antioxidative activity and inhibition of melanization. In conclusion, natural resources like LB extracts may be useful agents for the prevention of oxidative stress-mediated cytotoxicity and melanization by heavy metallic compounds such as LA.