Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Protective Effects of Lespedeza bicolor Extract on B16/F10 Melanoma Cell Lines Damaged by Lead Acetate, Heavy Metal Compounds

중금속 화합물인 초산납으로 손상된 B16/F10 멜라닌세포주에 대한 싸리나무 추출물의 보호 효과

  • Seo, Young-Mi (Department of Nursing, Wonkwang Health Science University)
  • Received : 2021.11.08
  • Accepted : 2021.12.05
  • Published : 2021.12.31
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Abstract

This study was conducted to evaluate the dermal cytotoxicity of lead acetate (LA) and other heavy metal compounds, and the protective effect of Lespedeza bicolor (LB) extract on LA-induced cytotoxicity in cultured B16-/F10 melanoma cells. The study evaluated the antioxidative effects of LB due to its electron-donating ability (EDA), inhibitory effects on melanization and improving cell viability. LA significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was determined at 52.7 µM in the studied cultures. Based on the Borenfreund and Puerner's toxicity criteria, LA was estimated to be highly cytotoxic. LA-induced cytotoxicity and cell damage was reversed by the antioxidant activity of kaempferol (KAE), thereby remarkably improving cell viability. A study of the protective effects of the LB extract on LA-induced cytotoxicity showed that the LB extract remarkably increased cell viability in the LA-treated group, and also inhibited the EDA and the total amount of melanin. The above results suggest oxidative stress-mediated cytotoxicity of LA. In the study, LB extract effectively prevented LA-induced cytotoxicity via its antioxidative activity and inhibition of melanization. In conclusion, natural resources like LB extracts may be useful agents for the prevention of oxidative stress-mediated cytotoxicity and melanization by heavy metallic compounds such as LA.

본 연구는 중금속화합물인 초산납(LA)의 피부독성을 B16/F10 멜라닌세포를 배양하여 조사하였으며, LA의 독성에 대한 싸리나무(Lespedeza bicolor, LB) 추출물의 보호효과를 조사하였다. 본 연구를 위하여 세포생존율을 비롯하여 항산화능 분석인 전자공여능(EDA)활성 억제능과 총 멜라닌량 저해능을 조사하였다. 그 결과 LA는 배양 세포에 처리한 농도 의존적으로 세포생존율을 유의하게 감소시킴으로써 독성을 나타냈다. 이 때 XTT50값은 52.7 µM로서 고독성(highly-toxic)인 것으로 나타났다. 한편, 항산화제인 kaempferol (KAE)은 LA의 독성에 손상된 세포생존율을 유의하게 증가시켰다. 또한, LA의 독성에 대한 LB 추출물의 보호효과에 있어서, LB 추출물은 LA만의 처리에 비하여 세포생존율을 유의하게 증가시켰으며, 이와 동시에 전자공여능(EDA) 활성 억제능과 멜라닌 저해능과 같은 항산화 효과 및 멜라닌화의 억제를 나타냈다. 이상의 결과로부터 LA의 독성에 산화적 손상이 관여하고 있으며, LB 추출물은 항산화와 멜라닌화의 저해 효과에 의하여 LA의 독성을 효과적으로 방어하였다. 따라서, LB 추출물과 같은 천연물질은 LA와 같이 산화적 손상과 관련이 있는 중금속화합물의 독성방어나 또는 멜라닌화로 인한 질환을 위한 치료 및 간호중재 보완물질로 개발할 가치가 있다고 생각된다. 본 연구결과를 기반으로 산화적 손상이 원인인 질환에 LB 추출물을 적용가능한 형태 및 방법에 대해 추가 연구가 필요함을 제언한다.

Keywords

Acknowledgement

This paper was supported by Wonkwang Health Science University in 2021.

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