• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1391-1400
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• 2018

Angelica tenuissima root has historically been used as a traditional medicine in Korea. Previous studies have identified the anti-melanogenic effects of the extract of A. tenuissima root fermented by Aspergillus oryzae (FAT). This study investigated the protective effects of FAT against ultraviolet light B exposure (UVB; $30mJ/cm^2$) in HaCaT (human keratinocyte) or Hs68 (human foreskin fibroblast) skin cells. FAT treatment was able to stimulate wound healing rate at the basal condition. FAT also favored the maintenance and/or improvement of extracellular matrix impairment caused by UVB irradiation through: 1) upregulation of procollagen Type-1 synthesis and secretion; 2) suppression of MMP-1 and elastase expression. FAT was able to play a role in the attenuation of inflammatory responses caused by UVB irradiation via upregulation of photo-protective hemeoxygease-1 and suppression of proinflammatory cyclooxygenase-2 expression. After further verification of the anti-photoaging potential of FAT, it could be utilized as an effective ingredient in anti-aging and anti-wrinkle cosmetics.

• 한국자원식물학회지
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• 제32권3호
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• pp.220-227
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• 2019

Stevia rebaudiana (Asteraceae), a perennial plant, has been used as a low-calorie sweetener and is being developed as a therapeutic agent for diabetes, hypertension, myocardial diseases, and microbial infections. Despite the common use of its leaves and stem, the bioavailability of the components present in S. rebaudiana flowers, when used as ingredients of cosmetics, has not been well investigated. Herein, we investigated the antioxidative and antimelanogenic effects of an aqueous extract of S. rebaudiana flowers (Stevia-F). Total flavonoid and phenolic content in Stevia-F were determined to be $8.64{\pm}0.23mg$ of quercetin equivalents/100 g and $631.5{\pm}2.01mg$ of gallic acid equivalents/100 g, respectively. The $IC_{50}$ values of Stevia-F for reducing power, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical, hydrogen peroxide, and nitric oxide scavenging activities were 5541.96, 131.39, 466.34, and $10.44{\mu}g/mL$, respectively. Stevia-F showed inhibitory effects on the tyrosinase ($IC_{50}=134.74{\mu}g/mL$) and ${\alpha}$-glucosidase ($IC_{50}=114.81{\mu}g/mL$) activities. No significant cytotoxicity of Stevia-F was observed in B16F10 cells, treated with up to $100{\mu}g/mL$ of the extract for 24 and 48 h (p > 0.05). Stevia-F ($1-100{\mu}g/mL$) suppressed ${\alpha}$-melanocyte stimulating hormone-induced melanin production in B16F10 cells (p < 0.05) and also inhibited the cellular tyrosinase activity (p < 0.05). Overall, our results show that Stevia-F possesses potential for inhibiting tyrosinase and ${\alpha}$-glucosidase activities and has significant antioxidant capacity. The antimelanogenic potential of Stevia-F should extend the usage of S. rebaudiana flowers in the development of skin-whitening products.

• 대한한방부인과학회지
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• 제28권3호
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• pp.74-86
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• 2015

Objectives To identify whether Sovereign-Minister-Assistant-Courier (君臣佐使) Theory and Meridian Tropism (歸經) Theory of oral herbal medicine can be applied to trans-dermal medicine and whether pharmaceutical efficacy can be delivered to skin by Courier herb (使藥). Methods We reviewed previous studies related to Sovereign-Minister-Assistant-Courier Theory. And we examined whether prescription compositions based on Sovereign-Minister-Assistant-Courier Theory correspond with efficacy results of trans-dermal medicine experimentally identified. Results When Sovereign-Minister-Assistant-Courier Theory is applied to trans-dermal medicine, Courier herb act as delivering the effective components to the lesion penetrating skin barrier. And we identified that medicine absorbed through the digestive processes may not correspond with Courier herb applied topically. Conclusions We identified that Courier herb act as delivering the effective components to the lesion. But to strength evidences, additional studies on this action will be needed.

• 대한한의학방제학회지
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• 제28권1호
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• pp.41-51
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• 2020

Objectives : US extract is a mixture of each extract of Ulmi cortex and Smilacis rhizoma. In this study, we investigated the antioxidant, anti-inflammatory, antibacterial, and ovoprotective effects of US extract in in vitro model to identify potential candidates for improving female reproductive function. Methods : The antioxidant activity of US extract was measured using 1,1-diphenyl- 2-picrylhydrazyl free radical and superoxide anion radical scavenging assays. The anti-inflammatory effect of US extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were determined with a nitric oxide (NO) assay, enzyme linked immunosorbent assays, and western blots analysis. The antibacterial activity of US extract against vaginitis infection microorganisms were determined with disc diffusion and minimum inhibitory concentration assays. The ovoprotective effect of US extract on 4-vinylcyclohexene diepoxide (VCD)-induced ovotoxicity in CHO-K1 cells were evaluated with a cell viability assay. Result : US extract showed good antioxidant capacity and inhibited LPS-induced NO production as well as iNOS and COX-2 expression and secretion of pro-inflammatory cytokine IL-6 without affecting the cell viability. It showed significant clear zones for Staphylococcus aureus and Candida albicans but did not indicate the clear zones for Escherichia coli and Enterococcus faecium. VCD-induced ovotoxicity in CHO-K1 cells was significantly reduced by US extract pre-treatment. Conclusions : These results demonstrate that US extract has antioxidant activity, anti-inflammatory effects on the LPS-stimulated macrophages, antibacterial activity against vaginitis infection microorganisms, and protective effects on the ovarian cells against VCD-induced ovotoxicity. These findings suggest that the US extract can be used as new prescriptions, supplements, functional foods, and cosmetics for improving female reproductive function.

• 동의생리병리학회지
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• 제26권3호
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• pp.325-329
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• 2012

The present study investigated the antimicrobial properties of medicinal herbs including Scutellariae Radix (SR: dried root of Scutellariae bicalensis Georgi). Among hot-water extracts of medicinal herbs tested in this study, SR extract showed the most potent antimicrobial activity with minimum inhibitory concentration (MIC) of 0.625 mg/mL. In particular, synergistic effects of antimicrobial activity were observed upon combined application of SR and chitooligosaccharide as indicated by MIC of 0.125 mg/mL and FIC (fractional inhibitory concentration) index of 0.45. Thermal stability analysis indicated that the components responsible for antimicrobial activity was stable for 8 months at $45^{\circ}C$. Antimicrobial activity was proven to be effective in foods as well as in cosmetics as comparable to that of the chemical preservatives.

• 한국자원식물학회지
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• 제30권4호
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• pp.466-474
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• 2017

오미자(Schizandra chinensis Baillon)는 국내 최대 생산량을 갖는 약용작물로서 음료, 기능성 식품 등에서 다양한 방법으로 사용되어왔다. 우리는 오미자와 관련된 한국의 객관적인 기술수준과 경쟁력을 파악하기 위하여 6개국(그룹)의 1,938건 유효특허를 수집하고 10분야의 핵심기술로 분류하였다. 오미자 관련기술은 한국이 1,182건으로 가장 많은 출원을 하였으며, 전체적으로 출원건수와 출원인수가 함께 증가하는 성장기 단계에 해당하였다. 특허 포트폴리오 모델, 지식재산권 부상도 분석 및 특허지표에 의하여 평가된 국내 기술수준과 경쟁력은 개별적인 질적 기술수준은 낮지만 특허출원건수가 많아 전체적인 기술경쟁력은 높다고 분석되었다. 우리는 본 연구를 통하여 오미자의 산업화 및 관련 연구개발 방향을 제시하고자 하였으며, 향후 한국에서 미래 선점 가능성이 가장 높은 기술 분야는 공백기술이 많고, 발전성은 높으며, 장벽성은 보통인 화장품과 재배 기술이 될 것으로 전망하였다.

• 한방안이비인후피부과학회지
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• 제28권4호
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• pp.111-121
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• 2015

Objective : The aim of this study is to assess the effect of Korean Herbal Cosmetic on quality of life (QoL) of Korean Herbal Cosmetics Using Dermatology Life Quality Index (DLQI) stratified by blind versus non-blinded option.Methods : Forty five healthy females aged 30's to 40's were recruited for this study. Volunteers were divided into two groups : Group A (n=22) was provided an anti-aging cream with ginseng extract in the original packaging including the brand name and logo. Group B (n=23) were provided same cream in a plain white normal jar without any package decoration or logo.Results : All females except two volunteers in group A completed a DLQI questionnaire, baseline, after 4 weeks and 8 weeks of treatment. The baseline of DLQI scores of all groups was 3.23±2.72. There was a significant difference in DLQI scores between before, 4 weeks and 8 weeks after in both groups ; The scores changed from 4.25±3.45 to 0.95±1.15 at 4 weeks, 1.00±1.72 at 8 weeks in group A, The scores of group B changed from 2.35±1.47 to 0.83±1.23 at 4 weeks, 0.65±0.98 at 8 weeks. But both had no interaction effect between follow up times and groups. Subscale DLQI scores improved after 4weeks were 'Symptoms and feelings', only in group BConclusions : Both groups showed significant improvements in QoL quality scores evaluated by DLQI. However, interaction was not observed for whether the participants knew the brand and content of the cream.

• 한국응용과학기술학회지
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• 제40권1호
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• pp.35-47
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• 2023

본 연구는 복합추출물(익모초, 어성초, 진피)의 생리활성을 측정하여 식·의약품, 화장품 등의 소재로서의 가능성을 확인하고자 하였다. 복합추출물은 열수 추출하였으며, 세포독성, 항산화, 항염증, MAPKs 등의 평가는 ELISA 및 western blot을 통해 확인하였다. 그 결과, 총 폴리페놀 함량은 35.53±0.48 mg GAE/g, 플라보노이드 함량은 8.18±0.16 mg QE/g으로 나타났으며, DPPH 와 ABTS radical 소거능은 농도 의존적으로 증가하였다. 대조군과 비교하여 세포독성은 400 ㎍/㎖ 농도까지 100% 이상의 생존율이 나타났으며, NO 및 염증성 사이토카인을 유의하게 감소시켰다. 또한, 복합추출물은 MAPKs(ERK, JNK, p38) 단백질 발현을 유의하게 억제시켰다. 이러한 결과는 복합추출물이 산화적 손상 및 염증에 사용될 수 있음을 나타낸다. 따라서 본 연구 결과는 식·의약품, 화장품용 복합소재의의 기초자료로 활용될 수 있을 것이다.

• 한국약용작물학회지
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• 제24권3호
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• pp.191-197
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• 2016

Background: This study examined the use of new bio-materials with enhanced value and functionality, which were derived from fermented wild ginseng cultures. Methods and Results: To examine the antioxidant activity associated with biological functions, radical scavenging analyses (2,2-diphenyl-1-picrylhydrazyl, DPPH and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, ABTS) and superoxide dismutase (SOD)-like activity analyses were conducted. Furthermore, the total phenolic and flavonoid contents of wild ginseng fermented with microorganisms (Leuconostoc mesenteroides, Bacillus circulans, Bacillus licheniformis and B. subtilis subsp. inaquosorum) were evaluated to determine the antioxidant activity increment. Regarding ginseng fermented with B. licheniformis, values of $70.6{\pm}1.4%$, $44.3{\pm}1.7%$, and $88.4{\pm}1.3%$ were measured using DPPH, ABTS, and SOD-like antioxdiant activity analyses, respectively. The total phenolic content in ginseng fermented with B. licheniformis was $184.5{\pm}0.9{\mu}g{\cdot}GAE/m{\ell}$, and the total flavonoid contents was $108.5{\pm}1.8{\mu}g{\cdot}QE/m{\ell}$ in ginseng fermented with L. mesenteroides. Conclusions: Of the four types of lactic acid bacteria examined, the use of B. licheniformis to ferment ginseng resulted in greatest increase in antioxidant activity. Therefore, ginseng fermented by microorganisms might be used to produce functional bio-materials.

• 동의생리병리학회지
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• 제28권6호
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• pp.636-642
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• 2014

In the theory of Korean medicine, Angelicae Dahuricae Radix (ADR) can expel wind and relieve exterior syndrome, and eliminate dampness. Recently, ADR has been reported to have possibilities as anti-inflammatory agent and cosmetics. The purpose of this study is to evaluate the efficacy of ADR on contact dermatitis (CD). In order to investigate the anti-inflammatory effects of ADR on CD, we investigated the effects of ADR on ear thickness, ear weight, skin lesion and histopathological changes in mice with CD induced by 1-fluoro-2,4-dinitrofluorobenzene (DNFB). In addition, the effect on spleen weight was also measured. In our results, topical application of ADR lowered ear thickness and weight respectively. ADR treatment also improved skin lesions such as erythema and scale. In the histopathological observation, ADR-treated group showed diminished epidermal hyperplasia and immune cell infiltration in inflammed tissues compared to those of non-treated control group. In conclusion, These data suggest that ADR has anti-inflammatory action in inflammed skin tissue, resulting in improving skin lesion and histopathological abnormalities of CD.