Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of Anti-inflammatory and Antioxidant Abilities of Complex Extracts Produced from Leonurus japonicus Houtt., Houttuynia cordata Thunberg, and Citrus unshiu Markovich

익모초, 어성초, 진피 복합 추출물의 항염증 및 항산화 평가

  • Hun Gyu Lee (Department of Oriental Medicine Resources Graduate School of Joongbu University) ;
  • Joong-Gu Ji (Department of Herbal Health & Pharmacy, Joongbu University)
  • 이훈규 (중부대학교 대학원 한약자원학과) ;
  • 지중구 (중부대학교 한방보건제약학과)
  • Received : 2023.02.09
  • Accepted : 2023.02.21
  • Published : 2023.02.28
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Abstract

This study aimed to confirm the potential as a material for food, medicine, and cosmetics by measuring the physiological activity of the complex extract(Leonurus japonicus Houtt., Houttuynia cordata Thunberg and Citrus unshiu Markovich). The complex extract was with hot water, The evaluation of cytotoxicity, antioxidant, anti-inflammatory, and MAPKs of the extract was performed by using ELISA and western blot. Total polyphenols and flavonoid contents of complex extracts were 126.16 mg/g and 105.84 mg/g, respectively. The scavenging activities of DPPH and ABTS radical significantly increased in a dose-dependent in complex extracts. Compared to the control group, the complex extracts treatments significantly reduced concentrations of nitric oxide and inflammatory cytokine (i.e., IL-1β, TNF-α, and IL-6). Furthermore, the complex extracts treatments significantly reduced protein expression levels of MAPKs (i.e., ERK, JNK, and p38). The results indicate that the complex extract can be used for oxidative damage and inflammation. Thus, the results of this study can be used as basic data for composite materials for food, medicine, and cosmetics.

본 연구는 복합추출물(익모초, 어성초, 진피)의 생리활성을 측정하여 식·의약품, 화장품 등의 소재로서의 가능성을 확인하고자 하였다. 복합추출물은 열수 추출하였으며, 세포독성, 항산화, 항염증, MAPKs 등의 평가는 ELISA 및 western blot을 통해 확인하였다. 그 결과, 총 폴리페놀 함량은 35.53±0.48 mg GAE/g, 플라보노이드 함량은 8.18±0.16 mg QE/g으로 나타났으며, DPPH 와 ABTS radical 소거능은 농도 의존적으로 증가하였다. 대조군과 비교하여 세포독성은 400 ㎍/㎖ 농도까지 100% 이상의 생존율이 나타났으며, NO 및 염증성 사이토카인을 유의하게 감소시켰다. 또한, 복합추출물은 MAPKs(ERK, JNK, p38) 단백질 발현을 유의하게 억제시켰다. 이러한 결과는 복합추출물이 산화적 손상 및 염증에 사용될 수 있음을 나타낸다. 따라서 본 연구 결과는 식·의약품, 화장품용 복합소재의의 기초자료로 활용될 수 있을 것이다.

Keywords

References

  1. W. H. Kim, "Gastrointestinal Side Effects of NSAIDs", Medical Postgraduates, Vol.29, No.6 pp. 333-347, (2001). 
  2. S. I. Oh, M. S. Lee, "Functional activities of ethanol extracts from Flammulina velutipes", Korean J Food Nutr, Vol.23, No.1 pp. 15-22, (2010). 
  3. H. W. Kang, "Antioxidant and anti-inflammatory effect of extracts from Flammulina velutipes (Curtis) Singer", Journal of the Korean Society of Food Science and Nutrition, Vol.41, No.8 pp. 1072-1078, (2012).  https://doi.org/10.3746/jkfn.2012.41.8.1072
  4. Branen, A. L., "Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene", Journal of the American Oil Chemists' Society, Vol.52, No.2 pp. 59-63, (1975).  https://doi.org/10.1007/BF02901825
  5. H. S. Kim & Y. H. Kang, "Antioxidant Activity of Ethanol Extracts of Non-Edible Parts (stalk, stem.leaf, seed) from Oriental Melon", Korean Journal of Plant Resources. Vol.23, No.5 pp. 451-457, (2010). 
  6. K. S. Kim, "Clinical Use of Non-Steroidal Anti-Inflammatory Drugs", Korean J Otorhinolaryngol-Head Neck Surg, Vol.47, No.2 pp. 91-98, (2004). 
  7. K. E. Lee, E. S. Lee and S. G. Kang, "Antioxidant and Tyrosinase Inhibitory Effects of the Extract Mixtures of Perilla frutescens", Houttuynia cordata and Camellia sinensis, Vol.41, No.2 pp. 173-180, (2015).  https://doi.org/10.15230/SCSK.2015.41.2.173
  8. Williams, G. M., C. X. Wang & Iatropoulos, M. J., "Toxicity studies of butylated hydroxyanisole and butylated hydroxytoluene. II. Chronic feeding studies", Food and chemical toxicology, Vol.28, No.12 pp. 799-806, (1990).  https://doi.org/10.1016/0278-6915(90)90052-O
  9. X. H. Shang, X. Pan, H. Wang and M. Li, "Leonurus japonicus Houtt.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine", J. Ethnopharmacol, Vol.152, pp. 14-32, (2014).  https://doi.org/10.1016/j.jep.2013.12.052
  10. Y. Z. Zhu, W. Wu, Q. Zhu, & X. Liu, Discovery of Leonuri and therapeutical applications: from bench to bedside. Pharmacology & therapeutics, Vol.188, pp.26-35. (2018).  https://doi.org/10.1016/j.pharmthera.2018.01.006
  11. Wang, C., Lv, X., Liu, W., Liu, S., & Sun, Z. Uncovering the pharmacological mechanism of motherwort (Leonurus japonicus Houtt.) for treating menstrual disorders: a systems pharmacology approach. Computational Biology and Chemistry, Vol.89, pp. 107384. (2020). 
  12. Chinese Pharmacopoeia Commission. The Pharmacopoeia of the People's Republic of China. 2020th ed. Beijing. China Medical Science Press. (2020). 
  13. Liu J, Peng C, Zhou QM, Guo L, Liu ZH, Xiong L. Alkaloids and flavonoid glycosides from the aerial parts of Leonurus japonicus and their opposite effects on uterine smooth muscle. Phytochemistry. Vol.145 pp. 128-136. (2018)  https://doi.org/10.1016/j.phytochem.2017.11.003
  14. H. Lu, X. Wu, Y. Liang & J. Zhang.. "Variation in chemical composition and antibacterial activities of essential oils from two species of Houttuynia THUNB", Chemical & pharmaceutical bulletin, Vol.54, No.7 pp. 936-940, (2006).  https://doi.org/10.1248/cpb.54.936
  15. Shingnaisui, K., Dey, T., Manna, P., & Kalita, J. Therapeutic potentials of Houttuynia cordata Thunb. against inflammation and oxidative stress A review. Journal of ethnopharmacology, Vol.220, pp. 35-43. (2018).  https://doi.org/10.1016/j.jep.2018.03.038
  16. Li T, Liu L, Wu H, Chen S, Zhu Q, Gao H, Yu X, Wang Y, Su W, Yao X, Peng T. Anti-herpes simplex virus type 1 activity of Houttuynoid A, aflavonoid from Houttuynia cordata Thunb. Antiviral Res. Vol.144, pp. 273-280. (2017).  https://doi.org/10.1016/j.antiviral.2017.06.010
  17. Hsu CC, Yang HT, Ho JJ, Yin MC, Hsu JY. Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice. Eur J Nutr. Vol.55, No.2 pp. 845-854. (2016).  https://doi.org/10.1007/s00394-015-0994-y
  18. M. T. Wang, K. V. Honn & D. Nie, "Cyclooxygenases, prostanoids, and tumor progression", Cancer and Metastasis Reviews, Vol.26, No.3 pp. 525-534, (2007).  https://doi.org/10.1007/s10555-007-9096-5
  19. E. H. Han, J. H. Park, J. Y. Kim & H. G. Jeong, "Houttuynia cordata water extract suppresses anaphylactic reaction and IgE-mediated allergic response by inhibiting multiple steps of FcepsilonRI signaling in mast cells", Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol.47, No.7 pp. 1659-1666, 
  20. T. Y. Lin, Y. C. Liu, J. R. Jheng, H. P. Tsai, J. T. Jan, W. R. Wong & J. T. Horng., "Anti-enterovirus 71 activity screening of Chinese herbs with anti-infection and inflammation activities", The American Journal of Chinese Medicin,. Vol.37, No.1 pp. 143-158, (2009). https://doi.org/10.1142/S0192415X09006734
  21. O. j. Kwon, A. R. Lee, S. S. Roh. "Improving Effects on Rats with Reflux Esophagitis Treated with Combined Extract of Young persimmon fruit and Citrus peel", The Korea Jounrnal of Herbology, Vol.31, No.1 pp. 25-31, (2016).  https://doi.org/10.6116/kjh.2016.31.1.25.
  22. J. Y. Cha & Y. S. Cho, "Biofunctional activities of citrus flavonoids". Applied Biological Chemistry.\, Vol.44, No.2 pp. 122-128, (2001). 
  23. Harborne J. B. & Williams C. A., "Advances in flavonoid research since 1992", Phytochemistry, Vol.55, No.6 pp. 481-504, (2000).  https://doi.org/10.1016/S0031-9422(00)00235-1
  24. Lu J, Zhang D, Zhang X, Sa R, Wang X, Wu H, Lin Z, Zhang B. Network Analysis of the Herb-Drug Interactions of Citrus Herbs Inspired by the "Grapefruit Juice Effect". ACS Omega. Vol.7, No.40, pp. 35911-35923. (2022).  https://doi.org/10.1021/acsomega.2c04579
  25. Dongxiao Su, Hesheng Liu, Xiangyang Qi, Lihong Dong, Ruifen Zhang, Jie Zhang, Citrus peel flavonoids improve lipid metabolism by inhibiting miR-33 and miR-122 expression in HepG2 cells, Bioscience, Biotechnology, and Biochemistry, Vol.83, No.9, pp. 1747-1755, (2019)  https://doi.org/10.1080/09168451.2019.1608807
  26. Faulds C & Williamson G. "The role of hydroxycinnamates in the plant cell wall", Journal of the science of food and agriculture, Vol.79, pp. 393-395, (1999).  https://doi.org/10.1002/(SICI)1097-0010(19990301)79:3<393::AID-JSFA261>3.0.CO;2-H
  27. Y. H. Kang, Y. K. Park & G. D. Lee, "The nitrite scavenging and electron donating ability of phenolic compounds", Korean journal of food science and technology, Vol.28, No.2 pp. 232-239, (1996). 
  28. M. H. Bang, J. C. Song, S. Y. Lee, N. K. Park & N. I. Baek, "Isolation and structure determination of antioxidants from the root of Paeonia lactiflora", Applied Biological Chemistry, Vol.42, No.2 pp. 170-175, (1999). 
  29. G. S. Kim, "Effect of Dietary Phenols on Body Tissue Oxidative State and Cancer Prevention", Korean J Food Nutr, Vol.10, No.1 pp. 74-81, (1997). 
  30. Panizzi L, Caponia C, Catalano S, Cioni P. L., Morelli I, "In vitro antimicrobial activity of extracts and isolated constituents of Rubusulmifolius", J Ethnopharmacol, Vol.79, pp. 165-168, (2002).  https://doi.org/10.1016/S0378-8741(01)00363-4
  31. Pekkarinen S. S., Stockmann H., Schwarz K, "Antioxidant activity and partitioning of phenolic acids in bulk and emulsified methyl linoleate", J Agric Food Chem, Vol.47, pp. 3036-3043, (1999).  https://doi.org/10.1021/jf9813236
  32. M. E. Yun, Y. S. Lee, Y. J. Lee, Y. M. Park, & S. N. Park,. "Antimicrobial, antioxidant and cellular protective effects of Houttuynia cordata extract and fraction. Applied Chemistry for Engineering, Vol.29, No.4. pp. 452-460 (2018)  https://doi.org/10.14478/ACE.2018.1035
  33. K. E. Lee, E. S. Lee and S. G. Kang, "Antioxidant and Tyrosinase Inhibitory Effects of the Extract Mixtures of Perilla frutescens". Houttuynia cordata and Camellia sinensis, Vol.41, No.2 pp. 173-180, (2015).  https://doi.org/10.15230/SCSK.2015.41.2.173
  34. J. E. Jeong, S. P. Shim, Y. S. Jeong, H. K. Jung, Y. C. Kim and J. H. Hong, "Optimization of Extraction Conditions for Ethanol Extracts from Citrus unshiu Peel by Response Surface Methodology", Korean J Food Preserv, Vol.18, No.5 pp. 755-763, (2011).  https://doi.org/10.11002/kjfp.2011.18.5.755
  35. Middleton E, Kandaswami C., "Potential health-promoting peoperties of citrus flavonoids", Inst Food Technol, Vol.48, pp. 115-119, (1994). 
  36. W. S. Woo, "Study of Natural Compounds and Chemistry", Seoul National University Press, Seoul. pp. 30-34,80-83, (2002). 
  37. Heim K. E., Tagliaferro A. R. & Bobilya D. J., "Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships", The Journal of nutritional biochemistry, Vol.13, No.10 pp. 572-584, (2002). 
  38. H. Y. Sohn, H. Y. Ryu, Y. J. Jang, H. S. Jang, Y. M. Park & S. Y. Kim, "Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera". Microbiology and Biotechnology Letters., Vol.36, No.3 pp. 195-200, (2008). 
  39. Tsao R., "Chemistry and biochemistry of dietary polyphenols", Nutrients, Vol.2, No.12 pp. 1231-1246, (2010).  https://doi.org/10.3390/nu2121231
  40. Williams R. J., Spencer J. P. & Rice-Evans C. "Flavonoids: antioxidants or signalling molecules?", Free radical biology and medicine, Vol.36, No.7 pp. 838-849, (2004).  https://doi.org/10.1016/j.freeradbiomed.2004.01.001
  41. H. J. Lim, H. J. Lee, M. H. Lim, "Comparison of Antioxidant and Anti-inflammatory Activity ofKorean Houttuynia cordata Thunb. Extracts", Journal of the Korean Applied Science and Technology, Vol.38, No.1 pp. 217-227, (2021).  https://doi.org/10.12925/JKOCS.2021.38.1.217
  42. J. G. Bak, J. Kim, & S. H. Ohk, "Preparation of Shark Byproduct Extract and Gellan Gum based Antibacterial Film Containing Green Tea Extract". Biomedical Science Letters,Vol.28, No.1. pp.50-57 (2022).  https://doi.org/10.15616/BSL.2022.28.1.50
  43. W. Y. Song, S. J. Byeon and J. H. Choi, "Anti-oxidativeand anti-inflammatory activities of Sasa borealis extracts", Journal of Agriculture & Life Sciences, Vol.49, pp. 145-154, (2015).  https://doi.org/10.14397/jals.2015.49.3.145
  44. G. Park, Y. Sim, W. Lee, S. H. Sung and M. S. Oh, "Protection on skin aging mediated by antiapoptosis effectsof the water lily (Nymphaea Tetragona Georgi) via reactiveoxygen species scavenging in human epidermal keratinocytes". Pharmacology, Vol.97, pp. 282-293, (2016).  https://doi.org/10.1159/000444022
  45. M. W. Byun & E. H. Byun. "Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts", Journal of the Korean Society of Food Science and Nutrition, Vol.44, No.2 pp. 182-190, (2015).  https://doi.org/10.3746/jkfn.2015.44.2.182
  46. J. H. Cha & E. M. Lim, "Effects of Gardeniae fructus on cytokines in mouse macrophage", The Journal of Korean Obstetrics and Gynecology, Vol.27, No.1 pp. 1-16, (2014).  https://doi.org/10.15204/JKOBGY2014.27.1.001
  47. Ripoll V. M., Meadows N. A., Raggatt L. J., Chang M. K., Pettit A. R., Cassady A. I. & Hume D. A., "Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMB", Gene. Vol.413, No.1 pp. 32-41, (2008).  https://doi.org/10.1016/j.gene.2008.01.014
  48. H. G. Kwon, J. S. Hwang, J. S. So & S. H. Lim, "Immunological homeostasis and inflammatory immune disorders", The Korean Society for Molecular and Cellular Biology, Vol.20, No.1 pp. 48-69, (2008). 
  49. Ferrero-Miliani L., Nielsen O. H., Andersen P. S. & Girardin S., "Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation", Clinical & Experimental Immunology, Vol.147, No.2 pp. 227-235, (2007).  https://doi.org/10.1111/j.1365-2249.2006.03261.x
  50. J. S. Chang, L. C. Chiang, C. C. Chen, L. T. Liu, K. C. Wang & C. C. Lin, "Antileukemic activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb", The American journal of Chinese medicine, Vol.29, No.2 pp. 303-312, (2001).  https://doi.org/10.1142/S0192415X01000320
  51. Delgado A. V., McManus A. T. & Chambers J. P., "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides. Vol.37, No.6 pp. 355-361, (2003).  https://doi.org/10.1016/j.npep.2003.09.005
  52. S. Y. Chae, M. J. Kim, D. S. Kim, J. E. Park, S. K. Jo & S. T. Yee, "Effect of Asterina pectinifera extracts on the activation of immune cells", Journal of the Korean Society of Food Science and Nutrition, Vol.36, No.3 pp. 269-275, (2007).  https://doi.org/10.3746/JKFN.2007.36.3.269
  53. Tracey, K. J., H. E. Wei, K. R. Manogue, Y. Fong, Hesse DG, Nguyen HT & Cerami A. "Cachectin/tumor necrosis factor induces cachexia, anemia, and inflammation", The Journal of experimental medicine, Vol.167, No.3 pp. 1211-1227, (1988).  https://doi.org/10.1084/jem.167.3.1211
  54. Beutler B & Cerami A., "The biology of cachectin/TNFα primary mediator of the host response", Annual review of immunology, Vol.7, pp. 625-655, (1989).  https://doi.org/10.1146/annurev.iy.07.040189.003205
  55. Caivano M., "Role of MAP kinase cascades in inducing arginine transporters and nitric oxide synthetase in RAW264 macrophages", FEBS letters, Vol.429, No.3 pp. 249-253, (1998).  https://doi.org/10.1016/S0014-5793(98)00578-X