• Journal of Plant Biotechnology
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• 제43권4호
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• pp.417-421
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• 2016

The complete chloroplast genome sequence of Panax ginseng breeding line 'G07006', showing higher salt tolerance, was confirmed by de novo assembly using whole genome next-generation sequences. The complete chloroplast (CP) genome size is 156,356 bp, including two inverted repeats (IRs) of 52,060 bp, separated by the large single-copy (LSC 86,174 bp) and the small single-copy (SSC 18,122 bp) regions. One hundred fourteen genes were annotated, including 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Among them, 18 sites were duplicated in the inverted repeat regions. By comparative analyses of the previously identified CP genome sequences of nine cultivars of P. ginseng and that of G07006, five useful SNPs were defined in this study. Since three of the five SNPs were cultivar-specific to Chunpoong and Sunhyang, they could be easily used for distinguishing from other ginseng accessions. However, on arranging SNPs according to their gene location, the G07006 genotype was 'GTGGA', which was distinct from other accessions. This complete chloroplast DNA sequence could be conducive to discrimination of the line G07006 (salt-tolerant) and further enhancement of the genetic improvement program for this important medicinal plant.

• 원예과학기술지
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• 제31권1호
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• pp.128-132
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• 2013

경남농업기술원 화훼연구소에서 2000년부터 교배조합 육성을 위하여 국내 재배농가로부터 유전자원을 수집한 후, 2003년 3월에 자주색 대륜계 'Hope'를 모본, 핑크색 대륜계의 'Sunnyeo'을 부본으로 인공교배를 실시하여 계통 육성 후, 2004년부터 2007년까지 특성검정을 실시한 결과 화색, 화형, 수확량 등 품질과 수량이 우수하다고 판단되어 2007년 농작물 직무육성 신품종선정위원회의 심의를 거쳐 'Party Time'으로 명명 품종등록(품종보호 제2691호) 하였다. 'Party Time'은 선명한 핑크색(RHS 65-C)과 갈색 화심(RHS 166-A)과의 조화가 선명한 반겹꽃의 대륜화이다. 또한 화형이 안정되고 화경이 강건하며 본당 수량은 연간 3회 검정 결과 평균 47.3개이며, 평균 절화수명은 12.7일이다.

• The Plant Pathology Journal
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• 제34권6호
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• pp.555-566
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• 2018

Two rhizobacteria Bacillus aryabhattai H26-2 and B. siamensis H30-3 were evaluated whether they are involved in stress tolerance against drought and high temperature as well as fungal infections in Chinese cabbage plants. Chinese cabbage seedlings cv. Ryeokgwang (spring cultivar) has shown better growth compared to cv. Buram-3-ho (autumn cultivar) under high temperature conditions in a greenhouse, whilst there was no difference in drought stress tolerance of the two cultivars. In vitro growth of B. aryabhattai H26-2 and B. siamensis H30-3 were differentially regulated under PEG 6000-induced drought stress at different growing temperatures (30, 40 and $50^{\circ}C$). Pretreatment with B. aryabhattai H26-2 and B. siamensis H30-3 enhanced the tolerance of Chinese cabbage seedlings to high temperature, but not to drought stress. It turns out that only B. siamensis H30-3 showed in vitro antifungal activities and in planta crop protection against two fungal pathogens Alternaria brassicicola and Colletotrichum higginsianum causing black spots and anthracnose on Chinese cabbage plants cv. Ryeokgwang, respectively. B. siamensis H30-3 brings several genes involved in production of cyclic lipopeptides in its genome and secreted hydrolytic enzymes like chitinase, protease and cellulase. B. siamensis H30-3 was found to produce siderophore, a high affinity iron-chelating compound. Expressions of BrChi1 and BrGST1 genes were up-regulated in Chinese cabbage leaves by B. siamensis H30-3. These findings suggest that integration of B. aryabhattai H26-2 and B. siamensis H30-3 in Chinese cabbage production system may increase productivity through improved plant growth under high temperature and crop protection against fungal pathogens.

• 원예과학기술지
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• 제34권3호
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• pp.433-441
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• 2016

Clubroot caused by the protist Plasmodiophora brassicae is one of the most destructive diseases of Brassica crops. Developing Chinese cabbage cultivars with durable clubroot resistance (CR) is an important goal of breeding programs, which will require new genetic resources to be identified and introduced. In this study, we evaluated resistance to P. brassicae race 4 using 26 Chinese cabbage (B. rapa ssp. pekinensis ) cultivars compared to the clubroot-susceptible Chinese cabbage inbred line 'BP079' and the clubroot-resistant European turnip (B. rapa ssp. rapifera ) inbred line 'IT033820'. No symptoms of clubroot disease were found in 'IT033820' infected with P. brassicae race 4, whereas the Chinese cabbage cultivars exhibited disease symptoms to various degrees. The Chinese cabbage cultivars that were reported to be clubroot-susceptible were susceptible to P. brassicae race 4; however, seven of the 20 cultivars reported to be clubroot-resistant were susceptible to this race of P. brassicae to varying degrees. Resting spores of P. brassicae were abundant within the infected root tissues of 'BP079', as revealed by light microscopy and scanning electron microscopy (SEM), but they were not detected in root tissues of 'IT033820'. Although resting spores were not detected by light microscopy in root tissues of the clubroot-resistant Chinese cabbage cultivar 'Kigokoro 75', a few spores were observed by SEM. The $F_1$ hybrids from a cross between 'IT033820' and 'BP079' showed no disease symptoms, and all $BC_1P_1$ progenies from a cross between the $F_1$ hybrid and 'IT033820' exhibited a resistance phenotype. In the $BC_1P_2$ population from a cross between the $F_1$ hybrid and 'BP079', this trait segregated at a ratio of 3(R):1(S) (${\chi}^2=1.333$, p = 0.248) at a 5% significance level. Inoculated $BC_1P_2$ plants were either highly resistant or highly susceptible to the pathogen, indicating that the CR to race 4 of P. brassicae carried by 'IT033820' is dominant. In the $F_2$ population, this trait segregated at a ratio of 15(R):1(S) (${\chi}^2=0.152$, p = 0.696) at a 5% significance level, suggesting that CR in 'IT033820' is mainly controlled by two dominant genes. Therefore, 'IT033820' represents a promising genetic resource for developing durable CR breeding lines in Chinese cabbage.

• 한국작물학회지
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• 제56권1호
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• pp.64-71
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• 2011

Health beneficial properties of soybean [Glycine max (L.) Merr.] isoflavones are well known. The objectives of this study were to determine and compare the isoflavone composition and concentrations of soybean varieties grown in different cultivated regions of Vietnam (i.e., upland and lowland). Total and individual isoflavone composition and concentrations were determined by high-performance liquid chromatography (HPLC). Total isoflavone concentrations varied from 1153 to $6604{\mu}g\;g^{-1}$ and averaging $3354{\mu}g\;g^{-1}$ across environments and varieties. In the lowland region, the highest total isoflavones concentration was observed in M103 cultivar ($5653{\mu}g\;g^{-1}$) and the lowest in VX9-3 ($1153{\mu}g\;g^{-1}$), whereas in the upland region the highest and lowest concentrations were in M103 ($6604{\mu}g\;g^{-1}$) and DT93 ($1938{\mu}g\;g^{-1}$), respectively. Across varieties, average total isoflavones concentration was higher in the upland than lowland region (3728 vs. $2980{\mu}g\;g^{-1}$). The malonylglucosides and acetylglucosides concentrations in upland soybean varieties were higher than those from the lowland region. Despite the presence of Genotype (G) x Environment (E) interactions, varieties with consistently high (M103) and low (VX9-3, DT93) isoflavone concentrations across environments were identified. This is the first report of isoflavones in Vietnamese soybean varieties, revealing large variation in isoflavones concentration and profile among different varieties and cultivated regions. Results will be useful in selecting high-isoflavones soybean varieties for growth in tropical regions.

• 한국응용곤충학회지
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• 제22권1호
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• pp.21-25
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• 1983

벼 잎집무늬마름병의 발생예찰방법을 개발하기 위하여 1976년부터 1981년까지 진흥과 유신을 공시하여 시험하였다. 그 결과 성숙기의 잎집무늬마름병 피해도는 7월중의 병반높이와 병반고율, 9월 11일의 병반지수에 의하여 추정할 수 있었다. 유신품종에 있어서 7월 11일의 최상위병반 높이와 성숙기의 피해도와는 관계는 $Y=4.64\chi-13.2,\;r=.840^{**}$이었다. 한편 약제방제시기와 회수시험결과는 잎집무늬마름병의 발병주율과 발병경율이 7월 11일에서 8월 1일 사이에 급격히 높아지는 사실과 7월중의 병반높이와 성숙기의 피해도와는 상관이 높은 것을 감안할 때 7월 15일과 7월 25일 또는 7월 25일과 8월 5일의 10일간격 2회방제가 가장 효과적이었다.

• 원예과학기술지
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• 제29권1호
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• pp.77-80
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• 2011

경남농업기술원 화훼연구소에서 황색 대륜계 '99-가-2'를 모본, 복색 중륜계 '99-나-3-1'을 부본으로 인공교배를 실시하여 복색 소륜계 거베라 'Sun Cap'을 육성하였다. 특성 검정 포장에 정식하여 2004년부터 2007년까지 3회에 걸쳐 생육 및 수량특성 검정을 실시하였다. 'Sun Cap'은 선명한적/황의 복색(RHS 45-B/9-A)과 녹색의 화심이 잘 조화되는 화경이 강건하고 안정된 화형의 홑꽃 소륜화이다. 년간 주당평균수량은 약 102.6본 정도이며, 평균 절화수명은 약 13.5일 정도이다.

• 한국육종학회지
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• 제42권1호
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• pp.35-39
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• 2010

Soybean proteins are widely used for human and animal feed in the world. ${\beta}$-conglycinin protein exhibits poor nutritional and food processing properties and Kunitz trypsin inhibitor (KTI) protein is a main anti-nutritional factor in soybean seed. The objective of this research was to identify the inheritance of $cgy_1$ gene and ti gene for the improvement of soybean cultivar with no KTI proteins and low amount of ${\beta}$-conglycinin. $F_2$ population was made by crossing between "Gaechuck2ho" (${\alpha}^{\prime}$-subunit present $Cgy_1Cgy_1$, KTI protein absent titi) and PI506876 (${\alpha}^{\prime}$-subunit absent $cgy_1cgy_1$, KTI protein present TiTi) parent. A total of 434 $F_2$ seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein and KTI protein using SDS-PAGE. The segregation ratio of 3 : 1 for $Cgy_1$ locus (310 $Cgy_1$_ : 124 $cgy_1cgy_1$) and Ti locus (339 Ti_ : 95 titi) were observed. Segregation ratios of 9 : 3 : 3 : 1 (241 $Cgy_1$_Ti_: 69 $Cgy_1$_titi: 98 $cgy_1cgy_1$Ti_: 26 $cgy_1cgy_1titi$) between $Cgy_1$ gene and Ti gene in $F_2$ seeds were also observed (${\chi}^2= 5.367$, P = 0.10 - 0.20). This data showed that $Cgy_1$ gene was inherited independently with the Ti gene in soybean. These results will be useful in breeding program for selecting the line that does not exhibit or lacks both ${\alpha}^{\prime}$-subunit protein and KTI protein in soybean.

• 생물환경조절학회지
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• 제18권4호
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• pp.377-384
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• 2009

본 연구는 시중에서 판매되는 고추 역병저항성 대목품종을 이용한 시설내 접목재배시 기존의 실생묘와 비교하여 공급양액의 EC 수준에 따른 고추 접목묘들의 생육과 생리적 반응을 알아보고자 하였다. 접목을 위한 대목으로 '카타구루마', '코네시안 핫', '탄탄'의 3가지 품종을 사용하였고, 대조구로 실생 품종 '녹광' 접수로 이용하였다. 공급양액의 EC 조건에 따른 접목묘와 실생묘의 생육과 생리적 반응 시험을 위해 공급 양액의 EC를 각각 1.5, 3.0, 5.0dS/m로 설정하여 비교시험을 수행하였다. 공급양액의 EC 수준에 따른 생육은 실생묘와 접목묘 모두 EC 수준이 높아질수록 억제되는 경향을 보였다. 접목묘 중 '카타구루마'가 EC 5.0dS/m에서 다른 품종들에 비해서 생육이 상대적으로 양호하였다. 단, EC 조건에 따른 생육은 재배시험 시기에 따라 다소 차이가 있었다. 무기양분의 흡수는 T-N과 P 농도는 EC 수준이 높을수록 증가하였으며, Ca과 Mg은 흡수량이 감소하였다. 광합성 능력은 실생묘는 EC 1.5와 3.0dS/m 수준간에 차이가 없으나, EC 5.0dS/m 수준에서 낮게 나타났다. 접목묘들도 EC 5.0dS/m 수준에서 광합성 능력이 낮아지는 결과를 보였다. SOD 활성도는 EC 수준에 따라 일정한 경향이 나타나지 않았으며, APX 활성도는 EC 5.0dS/m 수준에서 활성도가 높게 나타났다. 추후 더 다양한 대목들을 이용한 비교시험이 필요하며, 저온신장성과 내염성이 강한 대목 품종의 개발을 위한 연구의 필요성이 높다.

• 한국토양비료학회지
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• 제43권5호
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• pp.637-643
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• 2010

석회고토 및 패화석비료를 대체할 수 있는 토양개량제로 활용가치가 높은 굴껍질을 사과 '감홍' 재배 과수원에 시용하여 고두병 발생에 미치는 효과를 검토하였다. 석회고토와 굴껍질을 처리한구는 무처리구에 비해 표토와 심토의 토양 pH와 치환성 칼슘 함량이 증가된 반면, 치환성 나트륨 함량은 차이가 없었다. 사과의 고두병 발생비율은 무처리구가 10.4%로 가장 높았고 굴껍질 4 Mg$ha^{-1}$ 이상 처리할 경우 3.7% 이하로 유의적인 감소를 보였으며 석회고토 처리구는 8.9%로 무처리구와 비슷한 수준이었다. 굴껍질 시용량과 수확기 표토 및 심토의 pH와 치환성 칼슘 함량은 고두병 발병비율과 고도로 유의적인 부의상관을 보였다. 고두병 발병비율을 경감시킬 수 있는 굴껍질 시용량은 4 Mg $ha^{-1}$ 이지만 시험 후 토양의 치환성 칼슘 함량, pH 및 과실중량, 수량 등을 감안할 때 최적의 굴껍질 시용량은 2 Mg $ha^{-1}$로서 석회소요량과 같았다.