• Molecules and Cells
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• v.27 no.5
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• pp.601-608
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• 2009

The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK, and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans SK-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 $[M+H]^+$, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of metabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of $324[M+H]^+$. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.

• Korean Institute of Interior Design Journal
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• v.15 no.5 s.58
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• pp.44-51
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• 2006

Careful attentions should be paid on the renovation of historical building, not only because it conservations architectural heritages by affording new roles of the present days, but also determine their successive value in the stream of the preservation of cultural properties. Among drastically-increasing interest in the remodeling of the modem architecture in Korea, it is meaningful to research the cases in Japan since there are precedent, accumulative, and ample example of lengthened time. In this paper, two examples with different renovation concept, 'harmony' and 'confrontation' are compared. 'Minami-za' is the typical example to harmonize the present requirement with the original purpose and the memories of the buildings, where the use and the design are preserved. On the contrary, 'Shinpu-kan' is exemplified as confrontation where everything except the facade and the structure was remodeled. It is suggested to understand 'harmony' and 'confrontation' as a way to the coexistence of 'new' and 'old' on the basic of the historical meaning of architectures, not only as a design trend.

• Journal of architectural history
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• v.9 no.3 s.24
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• pp.7-22
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• 2000

As the educational function of Confucian school in the late Chosun dynasty had been declined, the other educational institution called YANGSAJAE was established. The founders of the institution was either local authorities or local Confucianists. Otherwise the institution was established by the cooperation of both local authorities and local Confucianists. YANGSAJAE began to be established from the 16th century. Most of them was founded in 1700s. In 1800s, it was not so difficult to see YANGSAJAE in many towns in Young nam and Honam provinces. The institution was located in separate places at the early stage of foundation. But since the middle of 1700s, the institution was located in or near the Confucian school. As a consequence, this influenced the location of Confucian schools in the late Chosun dynasty. In Youngnam province, the institution was mainly located near the Confucian school or in a separate place. On the contrary, most of the institution were constructed within Confucian school in Honam province. There were two types of the flat composition: one is based on hall and the other is based on room. The hall type, which had a hall in the middle and rooms in both sides, was general. The hall type usually had a size of 4-kan in width and 1.5-kan in length. The half-hipped roof was the general type and intimately related to the roof of Myung-Ryun Dang of local Confucian School.

• Journal of the Korean Society of Costume
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• v.44
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• pp.168-179
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• 1999

In order to settle down structural causes of the process of distribution and apparel production in domestic fashion industry, and to raise competitive power with changing market environment, we need to bulid up Quick Response(QR) system based on information technology such as EDI(Electronic Data Interchange), KAN(Korea Article Number), and POS(Point of Sales), POS is the most valuable information tool to promote QR system paractically. The purpose of this study was to investigate motivation of POS adoption, problems in operating the system, and satisfaction on POS effects. Data were collected by sending questionnaire to the managers in apparel firms which were using POS. Statistical devices were t-test, frequency with SAS program. The results fo the study were as follows: 1. The degree of satisfaction to POS effects was rather good. Especially, rapid evaluations on new products gave the greatest satisfaction to the users. 2. Satisfaction degree of POS effects according to introduces time and organization characteristic(number of employees) have no significant difference, but the firms which have higher annual sales volume showed higher satisfaction degree. Merchandise department showed higher satisfaction on POS effects. 3. The motivation of POS adoption was an effective logistics control with consumer information as a marketing strategy. The problems in operating the system were lack of investment, and professionals. They almost didn't have KAN code, instead, have their own code system.

• The Journal of Korean Medicine
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• v.15 no.2 s.28
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• pp.132-142
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• 1994

Paraquat has been widely used as a herbicide in Korea since 1970. Human ingestion results in transient impairment of liver and kidney function and a characteristically respiratory failure with a high mortality rate. Clinical investigation and Oriental Medical Treatment were done on 3 cases of paraquat poisoning admitted to the department of 1st Internal Medicine. Kyung San University Oriental Medical Hospital from August 1991 to December 1992. and following results were obtained. The symptoms of paraquat poisoning can be regarded as Sim hua chi song(心火熾盛), Kan hua sang yom(肝火上炎). um hu hua wang(陰虛火旺), Kan tam sup yol(肝膽濕熱), Yang myong yol tok(陽明熱毒) in Oriental Medicine. and the principle of treatment for paraquat poisoning can be applicable to Chong yol Ii sop(淸熱利濕), Chong yol hae tok(淸熱解毒). Sa ha hae tok(瀉下解毒). Ja um kang hua(滋陰降火) and so on. Three cases were treated by HuangYonHaeTokTang(黃蓮解毒湯) and KamDuTang(甘豆湯): all of them have survived without complications. According to the result above, it suggests that determination of treatment based on the differentiation of symptoms and signs(Pyon jung si chi ;辨證施治) of Oriental Medicine may prove to be helpful in saving lives of patients with paraquat poisoning.

• Journal of the Korean Institute of Educational Facilities
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• v.15 no.2
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• pp.27-38
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• 2008

This study aims to examine Japanese elementary school architecture in Meiji Era, in which Japanese modern education system was settled down and the school architecture flourished by mass construction of stereotyped school buildings. It is assumed that stereotyped school architecture is due to the influence of the standard drawings made by Japanese Ministry of Education, therefore standard drawings could be a key to understand the characteristics of the stereotyped school buildings in Meiji Era. In this perspective this study attempts to analyze 4 standard drawings and interpret it in relation to regulations and design guidelines for school architecture in that time. As results of this study the change and characteristics of stereotyped school architecture in Meiji Era are clarified in several categories. They are as follows : the deviation of classroom and corridor, the change of classroom size fixing to 4 kan(間) by 5 kan(間), the absolute use of north-lacing single corridor type block-plan, and the change of other space such as waiting room(控所), special instruction room, auditorium and gymnasium.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.454-458
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• 2006

HSF1 is the heat shock transcription factor in Saccharomyces cerevisiae. S. cerevisiae KNU5377 can ferment at high temperature such as $40^{\b{o}}C$. We have been the subjects of intense study because Hsf1p mediates gene expression not only to heat shock, but to a variety of cellular and environmental stress challenges. Basing these facts, we firstly tried to construct the hsf1 gene-deleted mutant. PCR-method for fast production of gene disruption cassette was introduced in a thermotolerant yeast S. cerevisiae KNU5377, which allowed the addition of short flanking homology region as short as 45 bp suffice to mediate homologous recombination to kanMX module. Such a cassette is composed of linking genomic DNA of target gene to the selectable marker kanMX4 that confers geneticin (G418) resistance in yeast. That module is extensively used for PCR-based gene replacement of target gene in the laboratory strains. We describe here the generation of hsf1 gene disruption construction using PCR product of selectable marker with primers that provide homology to the hsf1 gene following separation of haploid strain in wild type yeast S. cerevisiae KNU5377. Yeast deletion overview containing replace cassette module, deletion mutant construction and strain confirmation in this study used Saccharomyces Genome Deletion Project (http:://www-sequence.standard.edu/group/yeast_deletion_project). This mutant by genetic manipulation of wild type yeast KNU5377 strain will provide a good system for analyzing the research of the molecular biology underlying their physiology and metabolic process under fermentation and improvement of their fermentative properties.

• Journal of architectural history
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• v.5 no.2 s.10
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• pp.25-39
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• 1996

Architectural researches on the traditional houses of Korea have been studied mainly based on the data collected in the field survey. From explosively incresed real mesurements and drawings in the field, plenty of data have been collected. Those have been the basic data for verifing and developing the theories on the traditional house. But after Korean war the researchers in South-Korea were not able to approach to the field in North Korea, so the new data of North Korea were not added any more. The poverty of real data have caused regional unbalance in the researches. This paper aims at collecting new data of traditional house in North Korea. But still being prohibited for the researchers of South Korea to approach to the field, I had to depend on the memories and experiences of the immigrants from North Korea who are now living in Kyon-Nam and Pusan Province. Through the questionnaire and drawings, they described vivid memory of their old houses. I was able to collect the data of 71 cases, which are significant and valuable as much as those of the real field are. The data include the address and site condition, family structure, economic condition, construction period of each house, The drawings by themselves show the building forms and plans, the plans of each building, and the included spaces. Although the quantity of those data is not enough for statistical analysis, it shows general tendency for analizing regional charateristics, the differnces among economical classes, and the periodical change. It opens the way for verfying the existing theory. Analizing the data, I have some conclusions as followings: a. Most of researchers have classified the dominant housing type of Hamkyong-Do as 'the double fold' type. In this study, all cases of Hamkyongbuk-do also show 'the double fold plan with Chongju-kan'. But in Hamkyongnam-do some cases show 'the double fold plan without Chongju-kan, or projecting the stable into the yard, which seem to be different type from 'the double fold plan with Chongju-kan' b. Existing theories classified the dominant housing type of Pyongahn-do as 'two buildings with pararell arrangement'. This classification is verified with the plentiful cases in this study. Futhermore, I found new tendency, that is, getting higher econnomic condition, they construct annex buildings between the main buildings. Finally their houses show 'scattered ㅁ shape'. The houses included in this two types has narrow and closed inner yard, which is different from the houses of the same shape in the south region of Korea. c. Existing theories classified the dominant housing type of Hwanghae-do as 'ㅁ sape with Daechong' type. I found many cases of 'ㅁ shape', but only two cases show 'Daechong'. 'The doble fold' type was also founded. Unfortunately very few cases were sent from Hwanghae-do, it is not enough for finding general tendency.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.163-177
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• 2016

This study suggests a method for making a system of basic materials for the restoration of the 3X3 Kan central hall remains of a Buddhist temple from the Joseon Dynasty. Because there are both an extant hall and remains from the Joseon Dynasty, the basic material can be constructed through comparison with the floor plan. Multi-step comparisons were found to be the most suitable example for restoration. For this study, five parts of the 3x3 Kan remains were compared with 70 extant halls for a case study. For the first comparison, the limit of collection makes four examples in the whole examples. There are many relationships with the area and the length ratio of the front to the side as the comparison articles, so there are examples that satisfied both comparison articles. However, there is little relationship between the length ratio of the front Eokan to the front Hyeopkan and the length ratio of side Eokan to the side Hyeopkan. The construction method of the basic material shows the possibility of expansion with various directions that have pros and cons.

• Journal of the Korean Mathematical Society
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• v.33 no.4
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• pp.857-863
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• 1996

In 1954 M. H. Protter [1] formulated the following boundary value problem as an analogue of the plane Darboux problem.