• Journal of Life Science
• /
• v.29 no.10
• /
• pp.1086-1095
• /
• 2019

This research was performed to develop a new material consisting of a mixture of Red Allium cepa (RA) Cucurbita moschata duch (CM), and Angelica gigas Nakai (AG). RA and CM have low storage stability because of their high moisture content. Therefore, their major components were extracted and used for the research after a content analysis. In order to overcome these limitations, the quercetin from RA, ${\beta}-carotene$ from CM, and decursin/decursinol angelate (D/DA) from AG were separately extracted, and the biochemical activity of each extract and mixture was compared. RA was bioconverted by the Bacillus subtillis KJ-3 (BS3) after ethanol extraction. After bioconversion, the quercetin content of RA was increased by 128.9%. ${\beta}-carotene$ was detected in the CM ethanol extract and its content was very low concentrations at 0.2 mg/g. The AG ethanol extract (1 mg) contained 0.4146 mg and 0.3659 mg of D/DA, respectively. The purity of the D/DA was found to be about 78%. The flavonoid and polyphenol content of each extract and their mixtures (mixture 1 (RA:CM:AG = 5:2:3), mixture 2 (RA:CM: AG = 3:5:2), and mixture 3 (RA:CM:AG = 3:2:5)) were measured. In addition, the cell survival rate, anti-inflammatory activity, and antioxidant ability were also evaluated. In all the results, the antioxidant activity of mixture 3 was most effective. Therefore, these findings provide basic data for future food development using a 3:2:5 mixture of RA, CM, and AG.

• Korean Journal of Environmental Agriculture
• /
• v.18 no.4
• /
• pp.384-387
• /
• 1999

The residue of imidacloprid in hulled rice and paddy soil was investigated. In laboratory conditions, the degradation of imidacloprid in the soils followed first-order reaction kinetic. The rate of degradation was influenced by soil temperature and soil type. The half-life of imidacloprid at $18-28^{\circ}C$ was 66.7-96.3 days in the heavy clay soil and 56.8-117.5 days in the clay loam soil. Arrhenius activation energy obtained from the temperature experiment was 25.5 KJ/mol in heavy clay soil and 50.3 KJ/mol in clay loam soil. In paddy field, the degradation of imidacloprid was fast during the initial period but the degradation rate was gradually slow. About 10 % of the initial amount remained in the soil 120 day after the application. The residual amount of imidacloprid in rice was below the detection limit, 0.01 ppm. The residue level in rice was lower than MRL 0.05ppm in Korea.

• Advances in pediatric surgery
• /
• v.8 no.2
• /
• pp.101-106
• /
• 2002

The antiangiogenic effects of novel agent KJ3, Betulinic acid, and Fumagillin on the neovascularization were studied by examining ultrastructural alterations in the vasculature of synthetic gelform and mouse neuroblastoma C1300. Small pieces of gelform with 0.4% agar were introduced subcutaneously (s.c.) in 7 week old male CH3/HeJ mice. After the $LD_{50}s$ were determined by FACS analysis, a third of $LD_{50}$ of three drugs were injected either locally or intraperitoneally every other day for 14 days. A/J mice were inoculated s.c. with the C1300 neuroblastoma cell line, then either saline or three drugs were injected in the same manner. The antiangiogenic effects of three drugs were studied by measuring the histologic changes in tumors, and immunostaining for CD34, VIII/vWF, CD105, and thymidine phosphorylase. In the drug treated groups, the number of vessels in gelform experiments and C1300 neuroblastoma experiments were lower than the corresponding values in the control. The histologic findings were significantly different in drug treated groups on day 7, but these were not significant on day 14. These results imply that antiangiogenic agents were effective when the tumor burden is minimal.

• Journal of Life Science
• /
• v.8 no.3
• /
• pp.326-332
• /
• 1998

Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purifice enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and $60^{\circ}C$, respectively. The enzyme was stable at $50^{\circ}C$ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the $^{\alpha}$, $^{\beta}$, $^{\gamma}$-CD ratio of 42:46:12. Amylopectin was the most suitable substrate with 67% conversion to CD.

• Journal of Photoscience
• /
• v.6 no.1
• /
• pp.1-3
• /
• 1999

Solar ultraviolet-A (UV-A) irradiances indicent on a horizontal surface at Taegu of Korea during 1995-1998 were calculated with 5 min averages of measurements taken every 30 seconds by a broadband UV- A sensor. The maximum and minimum of monthly averages of daily UV-A dose were 499.37 KJ m-2 day-1 inJuly and 171.09 KJ m--2 day-1 in December for 4 years of the observation period. The maxima of daily UV-A dose and instandaneous UV-A were observed as 846.46KJ m-2 day-1 June 7, 1998 and 37.22 W m-2 at 12 : 15, July 16, 1998, respectively. Increasing trends in annual maxima of daily UV-A dose and instantaneous UV-A were averaged to 6.4% and 6.7% , respectively, per year during 1995-1998 at Taegu, Korea.

• Korean journal of applied entomology
• /
• v.23 no.3 s.60
• /
• pp.190-192
• /
• 1984

The 98 and 116 isolates of Pyricularia oryzae collected from Gyeong-nam Province in 1982 and 1983 were differentiated by using Korean differential rice cultivars. Twelve races from98 isolates and 11 races from 116 isolates of P. oryzae were classified indicating that KJ races group was $87\%$ and KI group was $13\%$ in total. The races KJ301 and KJ 401 were ca. $50\%$ in frequency among races identified.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.3
• /
• pp.397-404
• /
• 2013

Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at $40^{\circ}C$ and pH 7.4. Treatment with $Mg^{2+}$ and $Li^+$ showed a slight decrease in XynA activity; however, treatment with 5 mM $Cu^{2+}$ completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.

• Journal of the Korean earth science society
• /
• v.45 no.1
• /
• pp.37-47
• /
• 2024

In the summer of 2018, the Korea-Japan (KJ) region experienced an extremely severe and prolonged heatwave. This study examines the GloSea6 model's prediction performance for the 2018 KJ heatwave event and investigates how its prediction skill is related to large-scale circulation patterns identified by the k-means clustering method. Cluster 1 pattern is characterized by a KJ high-pressure anomaly, Cluster 2 pattern is distinguished by an Eastern European high-pressure anomaly, and Cluster 3 pattern is associated with a Pacific-Japan pattern-like anomaly. By analyzing the spatial correlation coefficients between these three identified circulation patterns and GloSea6 predictions, we assessed the contribution of each circulation pattern to the heatwave lifecycle. Our results show that the Eastern European high-pressure pattern, in particular, plays a significant role in predicting the evolution of the development and peak phases of the 2018 KJ heatwave approximately two weeks in advance. Furthermore, this study suggests that an accurate representation of large-scale atmospheric circulations in upstream regions is a key factor in seasonal forecast models for improving the predictability of extreme weather events, such as the 2018 KJ heatwave.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.17 no.3
• /
• pp.220-225
• /
• 1988

Rice and rice flour were stored at $13^{\circ}C$, $23^{\circ}C$ and $38^{\circ}C$ for 6 months and Quality indicator according to the correlation between physicochemical properties and sensory scores, was monitored. rat acidity was the most effective indicator for quality changes during storage and its increasing rate showed 1st-order reaction kinetics. The Arrhenius activation energies were 50,953 KJ/Kmol in rice and 59,750 KJ/Kmol in rice flour. On the basis of relation between the acceptable sensory score(3.0) and fat acidity, shelf-life of rice and rice flour were 378 and 358 days at $13^{\circ}C$ and 163 and 145 days at $23^{\circ}C$, respectively.

• Nuclear Engineering and Technology
• /
• v.12 no.3
• /
• pp.163-170
• /
• 1980

A Mather type plasms gun is operated at below 1 torr with a energy storage system (4KJ, 16.5KV, 35nH) to study the conditions of the efficient plasma focus. When the $D_2$ gas filling pressure is 0.18 torr and the stored energy is 3.8KJ, the discharge current of max. 180KA is obtained and the average axial velocity of the plasma is about $7cm/\mu\textrm{s}$. This is lower than the calculated velocity with above conditions by the snow-plow model. The discrepancy is due to the currents flowing over the insulator surface. The plasma focus occurs at low pressure compared with the results obtained by Bruzzone. The reasons are such that the plasma gun employed in this experiment is large for tile stored energy and the concentration of the residual gas is comparatively high. It is confirmed by a Long counter that the neutrons are generated from the dense plasma focus.