• 제목/요약/키워드: KGC

검색결과 51건 처리시간 0.021초

Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models

  • Ye Seul Son;Mijin Kwon;Naeun Son;Sang-Kyu Kim;Mi-Young Son
    • Journal of Microbiology and Biotechnology
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    • 제33권10호
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    • pp.1309-1316
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    • 2023
  • To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains (Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng, using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.

Biological Characteristics of the Ginseng Stem Fungus Gnat (Phytosciara procera) and Its Environmental-friendly Control Using Modified Topping of Ginseng Peduncles

  • Lee, Seung-Hwan;Shin, Jung-Sup;Hong, Tae-Gyun;Ahn, Young-Joon;Cho, Dae-Hui
    • Journal of Ginseng Research
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    • 제34권1호
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    • pp.23-29
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    • 2010
  • This study examined the biological characteristics of the insect and examined potential cultural controls using peduncle topping methods. Ginseng stem fungus gnat eggs hatched after 5 days; ecdysis lasted 3-4 weeks, and after 5 days pupation, adults emerged. Adults deposited eggs 1-2 days after emerging, and the entire life cycle lasted 32-40 days. The fungus gnats laid eggs $327\times220{\mu}m$ in size on cut planes of stems, but not on intact stem parts that had not been topped or wounded. Analyses of major weather data for the experimental areas and weather data for the past 30 years acquired from the Korea Meteorological Administration revealed that fungus gnat dispersion was prevalent under highly humid conditions and in areas with thick and frequent fogging. Among the topping times examined, fungus gnat damage to ginseng was lowest when topping occurred in late May. Among the five different topping methods evaluated on experimental ginseng farms, the cumulative fungus gnat damage to ginseng was low (0.8%) under partial peduncle topping (removal of peduncle with lateral fruit remaining) and removal of only flower buds (0.6%), with fungus gnat control effects of 82% and 86%, respectively, compared to conventional topping (removal of peduncle about 5 cm above its base). No fungus gnat damage to ginseng was observed under the no-topping treatment. These results suggest that partial topping of peduncles, while letting lateral fruits remain, is a potentially environmentally friendly method of controlling the ginseng stem fungus gnat.

미설명 도시가스의 분석 : 경인지역 중심으로 (A Study of Unaccounted-for Gas Volume : Seoul, Kyunggi Area)

  • 김수덕;서정규
    • 에너지공학
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    • 제12권4호
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    • pp.328-338
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    • 2003
  • 본 연구에서는 경인지역 도시가스회사들이 가스공사로부터 도매 매입한 도시가스의 양이 배관내재고 등의 기타 용도를 포함한 도시가스판매량과의 차이에 대해 검토하여 보고자 하였다. 1996년부터 2001년의 자료를 이용하여 가스공사로부터 매입한 물량, 배관길이, 판매자료, 손실, 자가소비, 기타의 항목으로 기존의 자료를 정리하였고, 이를 기초로 하여, 배관내 재고량 변화, 판매 자료에 기초한 추정 미검침 재고량과 그 이월분 등을 확인하였다. 또 이를 통해 우리의 관심의 대상이 되는 오차를 이해할 수 있는 틀을 제공하고, 사실관계의 확인을 실증적인 분석을 통해 제시하고자 하였다. 결과. 각 도시가스회사가 도매 매입한 물량이 미검침 재고량의 이월분, 배관내재고, 검침판매량, 손실, 자가소비, 기타를 합한 량보다 많다라는 통계적 가설을 기각함을 보인다.

3종의 배암차즈기에 대한 염증억제 활성 비교 및 활성성분 구명 (Anti-Inflammatory Activity of Three Kinds of Salvia and Its Active Compounds)

  • 신한재;곽효민;장미;박시환;민혜정;이정민;이문용;김종한;김성원;한창균;김승형
    • 한국약용작물학회지
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    • 제24권5호
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    • pp.401-407
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    • 2016
  • Background: Salvia has been widely cultivated for use in flavoring and folk medicines in many countries, including Korea and China. In this study, we investigated the anti-inflammatory activity and the underlying active compounds of Salvia extract and its fractions. Methods and Results: The anti-inflammatory activity was measured by assessing the inhibition of cysteinyl leukotriene production in rat basophilic leukemia (RBL)-2H3 mast cells. Salvia plebeia R. Br. was found to have the most potent inhibitory activity on leukotriene production than S. japonica and S. chanroenica had. Fifty percent ethanol extracts of S. plebeia R. Br. were successively partitioned with n-hexane, methylene chloride, ethyl acetate, 1-butanol and water. The ethyl acetate (EtOAc) fraction showed stronger anti-inflammatory activity than other solvent fractions did. The EtOAc fraction was subjected to silica gel column chromatography elution with a chloroform and methanol gradient system (100 : 1 ${\rightarrow}$ 1 : 1) yielding 10 fractions. Three kinds of fractions (chloroform:methanol = 20 : 1, 10 : 1 and 5 : 1) showed high inhibitory activity on leukotriene production. We confirmed the major compounds with anti-inflammatory activity from S. plebeia R. Br. Conclusions: In this study, the major components of S. plebeia that showed leukotriene production inhibitory activity were isolated using solvent extraction and silica gel column chromatography. Rosmarinic acid, hispidulin and luteolin were identified as the major compounds with anti-inflammatory effect.