Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models

  • Ye Seul Son (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Mijin Kwon (Laboratory of Efficacy Research, Korea ginseng Corporation) ;
  • Naeun Son (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Sang-Kyu Kim (Laboratory of Efficacy Research, Korea ginseng Corporation) ;
  • Mi-Young Son (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2023.05.15
  • Accepted : 2023.06.28
  • Published : 2023.10.28
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Abstract

To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains (Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng, using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.

Keywords

Acknowledgement

The authors would like to thank the chief of the KGC R&D headquarters, Man-Soo Park, and the division director, Dr. Seung-Ho Lee, for supporting this work.

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