• Plant Resources
• /
• v.8 no.2
• /
• pp.155-160
• /
• 2005

A full-length cDNA (PPrx1) encoding peroxidase has been isolated and its nucleotide sequence determined from flower bud in ginseng plant (Panax ginseng). A PPrx1 cDNA is 1192 nucleotides long and has an open reading frame of 1062 bp with a deduced amino acid sequence of 354 residues (pI 7.53). The deduced amino acid sequence of PPrx1 matched to the previously reported peroxidase protein genes. The PPrx1 showed a high similarity with the $64\%$ identity with peroxidase of N. tabacum (AAK52084). In the phylogenetic analysis based on the amino acid residues, the PPrx1 was closer with peroxidase of G. max (AAD37376).

• Journal of Microbiology and Biotechnology
• /
• v.28 no.5
• /
• pp.776-783
• /
• 2018

The agarase gene gaa16a was identified from a draft genome sequence of Gilvimarinus agarilyticus JEA5, an agar-utilizing marine bacterium. Recently, three agarase-producing bacteria, G. chinensis, G. polysaccharolyticus, and G. agarilyticus, in the genus Gilvimarinus were reported. However, there have been no reports of the molecular characteristics and biochemical properties of these agarases. In this study, we analyzed the molecular characteristics and biochemical properties of agarases in Gilvimarinus. Gaa16A comprised a 1,323-bp open reading frame encoding 441 amino acids. The predicted molecular mass and isoelectric point were 49 kDa and 4.9, respectively. The amino acid sequence of Gaa16A showed features typical of glycosyl hydrolase family 16 (GH16) ${\beta}$-agarases, including a GH16 domain, carbohydrate-binding region (RICIN domain), and signal peptide. Recombinant Gaa16A (excluding the signal peptide and carbohydrate-binding region, rGaa16A) was expressed as a fused protein with maltose-binding protein at its N-terminus in Escherichia coli. rGaa16A had maximum activity at $55^{\circ}C$ and pH 7.0 and 103 U/mg of specific activity in the presence of 2.5 mM $CaCl_2$. The enzyme hydrolyzed agarose to yield neoagarotetraose as the main product. This enzyme may be useful for industrial production of functional neoagaro-oligosaccharides.

• Steel and Composite Structures
• /
• v.24 no.2
• /
• pp.227-237
• /
• 2017

This paper presents optimization of a long-span portal steel frame under dynamic wind loads using a surrogate-assisted evolutionary algorithm. Long-span portal steel frames are often used in low-rise industrial and commercial buildings. The structure needs be able to resist the wind loads, and at the same time it should be as light as possible in order to be cost-effective. In this work, numerical model of a portal steel frame is constructed using structural analysis program (SAP2000), with the web-heights at five locations of I-sections of the columns and rafters as the decision variables. In order to evaluate the performance of a given design under dynamic wind loading, the equivalent static wind load (ESWL) is obtained from a database of wind pressures measured in wind tunnel tests. A modified formulation of the problem compared to the one available in the literature is also presented, considering additional design constraints for practicality. Evolutionary algorithms (EA) are often used to solve such non-linear, black-box problems, but when each design evaluation is computationally expensive (e.g., in this case a SAP2000 simulation), the time taken for optimization using EAs becomes untenable. To overcome this challenge, we employ a surrogate-assisted evolutionary algorithm (SAEA) to expedite the convergence towards the optimum design. The presented SAEA uses multiple spatially distributed surrogate models to approximate the simulations more accurately in lieu of commonly used single global surrogate models. Through rigorous numerical experiments, improvements in results and time savings obtained using SAEA over EA are demonstrated.

• Journal of Broadcast Engineering
• /
• v.19 no.2
• /
• pp.227-239
• /
• 2014

This paper proposes an enhance prediction for conventional near-lossless coder to effectively lower external memory bandwidth in image processing SoC. First, we utilize an already reconstructed green component as a base of predictor of the other color component because high correlation between RGB color components usually exists. Next, we can improve prediction performance by applying variable block size prediction. Lastly, we use minimum internal memory and improve a temporal prediction performance by using a template dictionary that is sampled in previous frame. Experimental results show that the proposed algorithm shows better performance than the previous works. Natural images have approximately 30% improvement in coding efficiency and CG images have 60% improvement on average.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.7 no.4
• /
• pp.613-631
• /
• 2013

Cognitive radio (CR) is proposed as a key solution to improve spectral efficiency and overcome the spectrum scarcity. Spectrum sensing is an important task in each CR system with the aim of identifying the spectrum holes and using them for secondary user's (SU) communications. Several conventional methods for spectrum sensing have been proposed such as energy detection, matched filter detection, etc. However, the main limitation of these classical methods is that the CR network is not able to communicate with its own base station during the spectrum sensing period and thus a fraction of the available primary frame cannot be exploited for data transmission. The other limitation in conventional methods is that the SU data frames should be synchronized with the primary network data frames. To overcome the above limitations, here, we propose a spectrum sensing technique based on blind source separation (BSS) that does not need time synchronization between the primary network and the CR. Moreover, by using the proposed technique, the SU can maintain its transmission with the base station even during spectrum sensing and thus higher rates are achieved by the CR network. Simulation results indicate that the proposed method outperforms the accuracy of conventional BSS-based spectrum sensing techniques.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1993.10a
• /
• pp.227-238
• /
• 1993

IRRI Engineering Division has developed a well drilling rig attachment that matched power tiller or hand tractor. It was designed in response to the growing demand for ground water utilization for small-scale irrigation, especially in drought-sticken and rained farms in Asian countries. The power tiller-driven rig can drill 30 meters of 100mm well in an unconsolidated formation in one day and can be rapidly converted from rotary to jetting or to the percussion method of drilling to suit different soil and rock formation. In addition, the power tiller can be quickly installed or removed from the rig frame and can be used for transporting the rig to other sites. The rig can be dismantled into smaller sub-assemblies for carrying by hand into less accessible areas. One manufacturer in Central luzon Philippines has started to produce the rig for well drillers in Central Luzon. The Department of Agriculture in the Philippines have procured thirty three(33) units of these machines f r their Shallow Tubewell program.

• Earthquakes and Structures
• /
• v.17 no.6
• /
• pp.599-609
• /
• 2019

After an earthquake, a quick seismic assessment of a structure can facilitate the recovery of operations, and consequently, improve structural resilience. Especially for facilities that play a key role in rescue or refuge efforts (e.g., hospitals and power facilities), or even economically important facilities (e.g., high-tech factories and financial centers), immediately resuming operations after disruptions resulting from an earthquake is critical. Therefore, this study proposes a prompt post-earthquake seismic evaluation method that uses displacement and acceleration measurements taken from real structural responses that resulted during an earthquake. With a prepared pre-earthquake capacity curve of a structure, the residual seismic capacity can be estimated using the residual roof drift ratio and stiffness. The proposed method was verified using a 6-story steel frame structure on a shaking table. The structure was damaged during a moderate earthquake, after which it collapsed completely during a severe earthquake. According to the experimental results, a reasonable estimation of the residual seismic capacity of structures can be performed using the proposed post-earthquake seismic evaluation method.

• Journal of the Korean Institute of Telematics and Electronics S
• /
• v.36S no.11
• /
• pp.9-14
• /
• 1999

In this paper, a method of one line transmission of 622Mbps to interface a low speed part with a high speed part is introduced instead of H-BUS method of the 10 line transmission of 77.76Mbps in the 10Gbps optical transmission system. For this method, a reframing method to align the received data of 622Mbps transmission to STM(Synchronous Transfer Mode)-64 frame of SDH(Synchronous Digital Hierarchy) is described. Reframing is designed with VHDL and applied in the 10G-S4 ASIC of T14U board of 10Gbps optical transmission system.

• Transactions of Materials Processing
• /
• v.18 no.3
• /
• pp.210-216
• /
• 2009

Springback, an elastic material recovery after the unloading of stamping tools, causes variations and inconsistencies of final part dimensions. Recently, narrow and long surround molding parts around door frame are applied to high grade automobiles, but there are great difficulties in their die development, construction and tryout because of several springback modes including vertical movement and twist during flange forming process of them. So it is very important to predict springback's quantities of a surround molding part and, moreover, to compensate the die for them adequately, when they can't be corrected by the restrike operation. This paper presents a case study based on the die design for a surround molding part made of stainless steel. The forming and springback predictions, carried out using PAMSTAMP 2G, are reported and compared with the measurement data of the prototype. The predicted springback results were acceptable, so the processes of compensating die using Automatic Die Compensation Module of PAMSTAMP 2G were performed iteratively until the tolerances between the designed shape and the simulation data were satisfied.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.5
• /
• pp.288-292
• /
• 2005

A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein-like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.