Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Molecular Cloning and Characterization of Type 2 Metallothionein cDNA from Codonopsis lanceolata (S. et Z.) Trautv

  • In, Jun-Gyo (Biopia Co., Ltd.) ;
  • Lee, Bum-Soo (Biopia Co., Ltd.) ;
  • Yi, Tae-Hoo (Dept. of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University) ;
  • Yu, Chang-Yeon (College of Agriculture and Lift Science, Kangwon National University) ;
  • Yang, Deok-Chun (Dept. of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University)
  • Published : 2005.10.30
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Abstract

A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein-like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.

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