• Biomolecules & Therapeutics
• /
• v.30 no.6
• /
• pp.529-539
• /
• 2022

Rheumatoid arthritis (RA) is a multifactorial immune-mediated disease, the pathogenesis of which involves different cell types. T-cell activation plays an important role in RA. Therefore, inhibiting T-cell activation is one of the current therapeutic strategies. Cytotoxic T-lymphocyte antigen 4-immunoglobulin (CTLA4-Ig), also known as abatacept, reduces cytokine secretion by inhibiting T-cell activation. To achieve a homeostatic therapeutic effect, CTLA4-Ig has to be administered repeatedly over several weeks, which limits its applicability in RA treatment. To overcome this limitation, we increased the number of sialic acid-capped antennas by genetically engineering the CTLA4 region to increase the therapeutic effect of CTLA4-Ig. N-acetylglucosaminyltransferase (GnT) and α2,6-sialyltransferase (α2,6-ST) were co-overexpressed in Chinese hamster ovary (CHO) cells to generate a highly sialylated CTLA4-Ig fusion protein, named ST6. The therapeutic and immunogenic effects of ST6 and CTLA4-Ig were compared. ST6 dose-dependently decreased paw edema in a mouse model of collagen-induced arthritis and reduced cytokine levels in a co-culture cell assay in a similar manner to CTLA4-Ig. ST6- and CTLA4-Ig-induced T cell-derived cytokines were examined in CD4 T cells isolated from peripheral blood mononuclear cells after cell killing through irradiation followed by flow- and magnetic-bead-assisted separation. Interestingly, compared to CTLA4-Ig, ST6 was substantially less immunogenic and more stable and durable. Our data suggest that ST6 can serve as a novel, less immunogenic therapeutic strategy for patients with RA.

• Journal of the Korean Institute of Gas
• /
• v.26 no.6
• /
• pp.24-29
• /
• 2022

This paper is a purpose to Analyze and study the failure examples for a engine oil leakage of camshaft bearing seal, poor contact by computer connector fin damage and vaporizer inferiority on LPG automotive. The first example, when the researcher disassembled the cylinder head of engine to establish the cause for oil leakage, he confirmed the engine oil leakage by damaged between the engine intake camshaft bearing and seal part. The second example, the connector fin of power source line that control the starting of a car supplied with engine computer. As a result, it found the fact that the engine operation stopped because of cutting of the power source by connector fin damage. The third example, it verified the engine incongruity phe cutting of the power source by connector fin damage. The third example, it verified the engine incongruity phenomenon as thd gas didn't flow the vaporizer by foreign substance deposit. Finally, it supplied a small quantity gas from vaporizer to mix. As the computer controlling mix opening condition supplied a air as opening signal, the air and fuel became rarefied state. it knew that the engine didn't produce prpper power. Therefore, a car have to throughtly inspect not in order to arise the failure symptoms.

• Journal of Mushroom
• /
• v.20 no.4
• /
• pp.258-262
• /
• 2022

Pleurotus species are the most consumed and cultivated mushrooms in Korea. Although oyster mushrooms (P. ostreatus) can be cultivated automatically, their storability is slightly lower than that of king oyster mushrooms (P. eryngii) and winter mushrooms (Flammulina velutipes); therefore, the export proportion of oyster mushrooms is very low. Since Korean mushrooms are highly preferred across Southeast Asian, the export of oyster mushrooms in the form of complete substrates is expected to be more promising than that of fresh mushroom. Here, 1 and 2.5 kg complete substrates of P. ostreatus 'Soltari' and P. sajor-caju 'Sambok' were prepared and stored at different temperature from 0 to 15℃ for 10 days. Thereafter, the formation of fruiting bodies was induced. Since the 2.5 kg complete substrates required 70 days of incubation, their mycelia were at an advanced age and their fruiting bodies did not grown normally. When 70%-incubated complete substrates were stored at 5-10℃, the growth was faster and more uniform and stable fruiting bodies were formed. Export test of complete substrates to Vietnam using distribution containers set at 0℃ and 15℃ revealed that the growth period was shortened by 1-2 days when the distribution containers were set at 15℃ and the yield of 'Soltari' increased by approximately 10%. In addition, even though the yield of 'Sambok' was similar between treatments at 0℃ and 15℃, the quality of fruiting bodies from 15℃-distributed complete substrates was much better than that of those from 0℃-distributed substrates.

• The Korea Journal of Herbology
• /
• v.29 no.2
• /
• pp.47-54
• /
• 2014

Objectives : This study was undertaken to verify the effect of Gangjihwan(Di-fatty, DF) composed with Pakistani Ephedra Herba on nonalcoholic fatty liver disease(NAFLD) using high fat diet-fed male mice. Method : Eight-week old C57BL/6N mice were used for all experiments. Standard chow diet-fed mice were used as normal group and high fat diet-fed NAFLD mice were randomly divided into 5 groups: control, atorvastatin, DF(1), DF(2) and DF(3). After 8 weeks, mice were treated with water, atorvastatin(10mg/kg) and DF(40, 80, 160mg/kg) for 8 weeks. And we investigated body weight gain, plasma lipid and glucose metabolism, histological analysis for liver on the mice. Results : Compared with controls, DF-treated mice had very significantly lower body weight gain and lower visceral adipose tissue weight, the magnitudes of which were prominent in DF(3). Consistent with their effects on body weight gain, DF-treated mice had lower blood total cholesterol and triglyceride level compared with controls. Consistent with their effects on body weight gain and blood plasma lipid level, DF-treated mice had lower liver weight and hepatic lipid accumulation of DF-treated groups was significantly decreased than control group. Also Blood plasma AST, ALT and ${\gamma}$-GT concentration were not changed by DF, and these results may indicate DF do not show any toxic effects. Conclusions : These results suggest that DF effectively improves NAFLD. DF reduces liver weight and prevents lipid accumulation of hepatocyte by reducing body weight gain and modulating blood plasma lipid metabolism levels.

• Biomolecules & Therapeutics
• /
• v.30 no.4
• /
• pp.340-347
• /
• 2022

Advanced or metastatic breast cancer affects multiple organs and is a leading cause of cancer-related death. Cancer metastasis is associated with epithelial-mesenchymal metastasis (EMT). However, the specific signals that induce and regulate EMT in carcinoma cells remain unclear. PRR16/Largen is a cell size regulator that is independent of mTOR and Hippo signalling pathways. However, little is known about the role PRR16 plays in the EMT process. We found that the expression of PRR16 was increased in mesenchymal breast cancer cell lines. PRR16 overexpression induced EMT in MCF7 breast cancer cells and enhances migration and invasion. To determine how PRR16 induces EMT, the binding proteins for PRR16 were screened, revealing that PRR16 binds to Abl interactor 2 (ABI2). We then investigated whether ABI2 is involved in EMT. Gene silencing of ABI2 induces EMT, leading to enhanced migration and invasion. ABI2 is a gene that codes for a protein that interacts with ABL proto-oncogene 1 (ABL1) kinase. Therefore, we investigated whether the change in ABI2 expression affected the activation of ABL1 kinase. The knockdown of ABI2 and PRR16 overexpression increased the phosphorylation of Y412 in ABL1 kinase. Our results suggest that PRR16 may be involved in EMT by binding to ABI2 and interfering with its inhibition of ABL1 kinase. This indicates that ABL1 kinase inhibitors may be potential therapeutic agents for the treatment of PRR16-related breast cancer.

• Membrane Journal
• /
• v.32 no.2
• /
• pp.133-139
• /
• 2022

As the issue of reducing greenhouse gases is emerging due to global warming and extreme weather, research on materials capable of radiative cooling without energy consumption is being actively conducted. Among them, silk is known as a natural self-cooling material, but in the conventional mixing process using chemically powdered silk, there is a problem that the radiative cooling effect disappears by the collapses of the intrinsic crystal structure of silk fibroin, so it is difficult to manufacture it in the form of a film or coating agent for radiative cooling. In this study, various types of membranes were manufactured using silk powder that went through a physical pulverization process that does not damage the intrinsic structure of silk fibroin, and the study was conducted to examine its applicability as a coating agent. Electrospun membranes and flat sheet membranes were prepared by using silk fibroin powder for this purpose, and it was observed that the viscosity of the solution had a significant effect on the membrane fabrication and its properties.

• Journal of the Korean Applied Science and Technology
• /
• v.38 no.6
• /
• pp.1512-1523
• /
• 2021

The purpose of this study was to investigate the effect of Saengmaeg-san intake on blood lipids and arteriosclerosis index in male tennis players. Seventeen male tennis players enrolled in university were assigned to a Saengmaeg-san intake group (n=9) and a placebo control group (n=8) according to whether they took Saengmaeg-san during 4-week high-intensity tennis training. Tennis training was conducted 5 times a week for 4 weeks, and the exercise intensity was performed at 70-90% of the spare heart rate. The intake of Saengmaeg-san was 110ml at a time before breakfast, before, during, during, and after exercise, and after dinner, a total of 7 times, 770ml per day. For all data, mean and standard deviation were used. Two-way repeated measures ANOVA was used to examine the effect of time and treatment, and Pearson's Correlation was used to examine the relevance of differences in blood lipid responses after treatment. As a result of this study, the Saengmaeg-san intake group significantly improved blood lipids (triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol) and arteriosclerosis index, and there was a significant correlation appeared between ∆TG, ∆LDL-C, and ∆TC. As a result, the intake of Saengmaeg-san during high-intensity training of male college student tennis players can have a positive effect on blood lipids and arteriosclerosis index, suggesting that it can be an effective sports drink as an exercise supplement.

• Journal of Mushroom
• /
• v.20 no.3
• /
• pp.153-162
• /
• 2022

The purpose of this study was to breed a variety of stable productivity and high storage characteristics of white Hypsizygus marmoreus, which has high demand at domestic and global market due to a low bitter taste and the preference for white species. Accordingly, 'Baekmaru' was bred by hybridizing brown and white cap color species and backcrossing with white species. The 'Baekmaru' variety was bred by crossbreeding the brown and white species and backcrossing the white species. Through repeated cultivation of 'Baekmaru', a variety with a low contamination rate was selected when culturing the spawn for stable cultivation. As a results of demonstration test, the yield of 'Baekmaru' was 14% higher than that of the commercial variety. In addition, among the fruiting body characteristics of 'Baekmaru', diameter and thickness of the pileus were 16.43±15.27mm and 6.46±0.58mm, which were slightly higher than the commercial variety, and the hardness was 2.69±0.89N for the pileus and 3.09±0.89N for the stipe. The shelf life showed less change in thickness and color of pileus compared to commercial variety. The hardness of pileus of 'Baekmaru' was maintained in the range of 3.5 to 4.0N in the 4℃ and 4℃ and 20℃ mixed treatment until 30 days of storage, and was higher than that of the commercial variety. Therefore, it was suggested to be excellent in storability.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.26 no.6
• /
• pp.73-81
• /
• 2022

Recently, as the service life of structures increased, the load-carrying capacity of deteriorated reinforced concrete, where corrosion of reinforcing bars occurs due to various causes, is frequently decreased. In order to address this problem, many studies on the bond characteristic of FRP (Fiber Reinforced Polymer) bars with corrosion resistance, light weight and high tensile strength have been conducted, however there are not many studies on the bond characteristic of grid-typed CFRP embedded in concrete. Therefore, in order to evaluate the bond characteristics of grid-typed CFRP and its usability as a substitute for steel rebar, a pull-out test is performed using the longitudinal bond length and transverse grid length of the grid-typed CFRP as variables. Through the pull-out test, the bond load-slip curve of the grid-typed CFRP is derived, and the bond behavior is analyzed. The total bond load equation is proposed as the sum of the bond force of the longitudinal bond length and the shear force of the grid in the transverse direction. Also, expressing the area of the bond load-slip curve as total work, the change in dissipated energy with respect to the slip is analyzed to examine the effect of the tranverse grid on the bond force.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.35 no.4
• /
• pp.91-104
• /
• 2022

Objectives: This study was performed to review the impact of Coronavirus disease 2019 (COVID-19) infection during pregnancy and the applicability of traditional Chinese medicine (TCM) on COVID-19 infection during pregnancy. Methods: We searched 6 data bases on August, 2022 and articles about the impact of COVID-19 infection during pregnancy, TCM treatment of COVID-19 infection, TCM treatment of pregnancy common cold (姙娠 感冒) or cough (姙娠 咳嗽) were reviewed. Results: COVID-19 infection can bring out negative effects both on pregnant women and fetus. In the case of COVID-19 infection during pregnancy, the symptoms and treatment are similar to those of general COVID-19 infection, but the safety of drug intervention has not been completely verified. Herbal medicine treatment can be applied according to the severity, stage and TCM syndrome types of COVID-19 infection, but the prohibited herbs list during pregnancy and its dosage should be checked carefully. Also, when it comes to pregnancy disease, the effect of "Stabilizing Fetus" should be considered in the perspective of TCM treatment strategy. Glycyrrhizae Radix et Rhizoma, Rhizoma Atractylodis Macrocephalae, Scutellariae Radix have been widely used on COVID-19 infection and the safety of them during pregnancy have been verified. In the case of acupuncture treatment, acupoints also can be selected according to the severity, symptoms, and TCM syndrome types of COVID-19, but acupoints with strong Qi sensitivity or acupoints located in lower abdomen should be avoided during pregnancy. Conclusions: TCM treatment can be applied to COVID-19 infection during pregnancy. Clinical studies and systematic reviews of the efficacy and safety of TCM treatment on COVID-19 infection during pregnancy are required.