• Research in Plant Disease
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• v.16 no.3
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• pp.316-319
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• 2010

The gray mold disease occurred on tuberous roots of yacon in storage facilities in Gangneung, Korea, in March 2010. Symptoms typically appeared as in the form of dark brown discoloration on the surface of tuberous roots and water-soaked brown lesions in cross sections of the affected portions. A total of five isolates of Botrytis sp. were obtained from the symptomatic portions. All isolates on potato-dextrose agar (PDA) produced abundant conidia which were pale brown, one-celled, mostly ellipsoid or ovoid in shape and $8.2{\sim}14.8{\times}6.5{\sim}9.9\;{\mu}m$ in size. Large numbers of round to irregular, smooth, black, hard sclerotia were produced on PDA over time. The optimal temperature for mycelial growth and sclerotia formation of the fungal isolates was $20^{\circ}C$. On the basis of morphological and cultural characteristics, all the fungal isolates were identified as Botrytis cinerea. Pathogenicity test on host plants showed that the fungus could infect not only tuberous roots but also leaves and petioles of yacon. This is the first report on gray mold of yacon (Smallanthus sonchifolius) caused by Botrytis cinerea in Korea.

• Journal of Veterinary Clinics
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• v.25 no.3
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• pp.207-210
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• 2008

Trichophyton erinacei is a dermatophyte pathogen that infects both humans and hedgehogs. A two-month old female four-toed hedgehog presented to the Chonbuk Animal Medical Center with pruritus, excoriation and crust on her face for ten days. The owner of the hedgehog also exhibited the clinical signs of scaly erythema with fine vesicles on her neck. A presumptive diagnosis of dermatophytosis was made based on the results of an acetate tape preparation in which hyphae and chains of arthroconidia were observed. The crusts from the lesions were then cultured on Sabouraud Dextrose Agar for identification. After 10 days of incubation, downy colored colonies that had a central umbo with a white granular surface and a yellow pigment ring in the reverse were observed. Microscopic analysis revealed the presence of numerous teardrop shaped microconidia singly attached to the sides of the hyphae. In addition, 2-6 roomed macroconidia that were somewhat irregular in shape and size were present, and abundant intermediate sized spores were observed between the micro and macro conidia. To confirm that the culture was T. erinacei, the internal transcribed spacer region of the 5.8S phase of the ribosomal RNA gene (ITS1-5.8S-ITS2 rDNA) was amplified by PCR and then sequenced. A 679-base pair fragment of DNA was then compared with sequences in GenBank and found to be 99% homologous with sequences of T. erinacei (Z97997 and Z97996. The clinical signs were resolved after four weeks of treatment with oral and topical ketoconazole and chlorhexidine. To the best of our knowledge, this represents the first case of T. erinacei isolated from a four-toed hedgehog in Korea.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.393-406
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• 2019

In August 2017, anthracnose symptoms were observed on the petioles and leaf veins of Korean radish (Rhaphanus sativus) in Hongcheon, Jeongseon, and Pyeongchang of the Gangwon province, Korea. Many grayish to dark-brown spots of 1-2 mm in diameter, appeared on the lower surface and leaf veins of the radish leaves. The spots gradually enlarged and coalesced to form dark-brown irregular lesions. Ten Colletotrichum isolates were obtained from the affected tissues of the Korean radish. Out of them, eight isolates were identified as C. higginsianum and two isolates were identified as C. truncatum based on morphological characteristics and multi-locus molecular phylogenetic analysis using the internal transcribed spacers and intervening 5.8S rDNA (ITS), partial beta-tubulin gene (TUB2), partial actin gene (ACT), and partial chitin synthase-1 gene (CHS1). The pathogenicity test was carried out on wounded and unwounded Korean radish (cv. Siraegimu and Osarimu), and Chinese cabbage (cv. Chuno and Smart) by inoculating with a spore suspension. All isolates except one C. truncatum isolate developed typical symptoms on both wounded and unwounded Korean radish. In Chinese cabbage, only the plants inoculated with C. higginsianum isolates developed symptoms regardless of the wound. This is the first report of anthracnose caused by C. truncatum on Korean radish in Korea.

• The Korean Journal of Mycology
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• v.30 no.2
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• pp.173-175
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• 2002

Sooty leaf blight was found on Dendrobium sp. in several farmers' fields located in Namji-eup, Changnyeong-gun, Gyeongnam province, Korea in 2001. Symptoms of the disease appeared on leaves. Sooty leaf spots were started with amphigenous, subeircular to irregular spots, with light grayish brown to black color with definite margin lines on the upper surface of leaves. Infected leaves became defoliated and whole plants eventually were died. The infection rates of the disease in the surveyed area reached up to 64.8%, in the early September. Conidiophores of the causal fungus were dark grayish brown in color, densely fasciculate, straight, curved to sinuous, branched, $5{\sim}10$ septate and $63{\sim}164{\times}3.2{\sim}4.9{\mu}m$ in size. Conidia were pale to olivaceous in color, obclavatecylindric, straight to slightly curved in shape, $1{\sim}5$ septate and $46{\sim}98{\times}3.2{\sim}3.9{\mu}m$ in size. The optimum temperature for mycelial growth of the fungus was $25^{\circ}C$. The fungus was identified as Pseudocercospora dendrobii on the basis of its mycological characteristics. This is the first report on sooty leaf light of Dendrobium sp. caused by Pseudocercospora dendrobii in Korea.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.2
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• pp.95-102
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• 2002

Spatial interpolation has become a common procedure in converting temperature forecasts and observations at irregular points for use in regional scale ecosystem modeling and the model based decision support systems for resource management. Neglection of terrain effects in most spatial interpolations for short term temperatures may cause erroneous results in mountainous regions, where the observation network hardly covers full features of the complicated terrain. A spatial interpolation model for daytime hourly temperature was formulated based on error analysis of unsampled site with respect to the site topography. The model has a solar irradiance correction scheme in addition to the common backbone of the lapse rate - corrected inverse distance weighting. The solar irradiance scheme calculates the direct, diffuse and reflected components of shortwave radiation over any surfaces based on the sun-slope geometry and compares the sum with that over a reference surface. The deviation from the reference radiation is used to calculate the temperature correction term by an empirical conversion formula between the solar energy and the air temperature on any sloped surfaces at an hourly time scale, which can be prepared seasonally for each land cover type. When this model was applied to a 14 km by 22 km mountainous region at a 10 m horizontal resolution, the estimated hourly temperature surfaces showed a better agreement with the observed distribution than those by a conventional method.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.9 no.3
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• pp.83-92
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• 2004

A continuous monitoring of textural characteristics of surface sediments, sedimentation rates and beach profile was carried out to investigate the seasonal variations of sedimentary processes in the Imjado beach, southwestern coast of Korea for two years. The beach profiles consist of steep beach face and relatively flat middle and low tide beaches. The slope of the beach face increases in summer and decreases in winter, in good accordance with the standard beach cycle. Ridge and runnel systems are well developed in the middle and low tide beaches during the summer, but these structures are replaced by mega-ripples during the winter. The sediments are fining southward as well as landward. The mean grain-size tends to be increasingly coarser during seasons of autumn and winter on the north beach and during seasons of winter and spring on the south one. In addition, the sediments are eroded on the north beach and accumulated on the south one as a whole. These are probably due to southward transportation of the sediments as long-shore current (NE-SW) runs around the coastal line of the beach. However, the seasonal variations in accumulation rates are very complex and irregular. It is considered that the Imjado beach represents in non-equilibrium state, as a result of coastal and submarine topographic changes by artificial agents and sea-level uprising associated with global warming.

• Research in Plant Disease
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• v.21 no.1
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• pp.36-39
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• 2015

In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was $4-10{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.56-56
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• 2003

Beta-catenin is very important in early development including involvement in cell adhesion, cell signaling, and developmental fate specification. Cell-cell interaction is an important process during mammalian embryonic development. In preimplantation embryos, embryonic compaction is the process of increased cellular flattening and adhesion of junctional complexes and results in a polarized distribution. Beta-catenin is associated with embryonic compaction in mammals. Here, we examined the relationship between beta-catenin expression and compaction in porcine embryos derived from in vitro fertilization. First of all, we investigated beta-catenin expression in each embryonic developmental stage and also focused on expression pattern according to full, partial and non-compaction at morula stage. We used the immunocyto-chemical method in this research. To confirm compaction affects on the embryonic development, we compared between compaction and developmental rates to the blastocyst. The result showed that compaction and non-compaction rates were 14.6% and 63.8% at 4 days after IVF, respectively The developmental rates to the blastocyst and their total cell number were 50.9% vs 36.4% and 41.4$\pm$11.5 vs 26.8$\pm$12.7 in compaction and non-compaction groups. Although no difference was detected in the ratio of ICM to total cells between two groups, total cell number of the blastocysts in compaction group was superior to that of the blastocysts in non-compaction group (P<0.05). Expression of beta-catenin appeared in the boundary of membrane surface between blastomeres in 2- and 4-cell stage, and observed irregular pattern from 8-cell to blastocyst stage. We also investigated beta-catenin expression pattern according to the degree of compaction in the 3 groups; full, partial (>50%) and non-compaction. The expression signal in fully compacted embryos was stronger than those of partial and non-compacted embryos. Especially, beta-catenin expression appeared various patterns in morula stage suggesting the aberrant distribution of beta-catenin is affected by compaction patterns. Our results suggest that abnormal beta-catenin expression was affected by embryo quality and further development in porcine embryos in vitro.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.202-209
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• 1998

A three-dimensional numerical method based on the Green's integral equation is developed to predict the motion response and drift force in waves for the ocean monitoring facilities. In this method, we use source and doublet distribution, and triangular and rectangular eliments. To eliminate the irregular frequency phenomenon, the method of improved integral equation is applied and the time-mean drift force is calculated by the method of direct pressure integration over the body surface. To conform the validity of the present numerical method, some calculations for the floating sphere are performed and it is shown that the present method provides sufficiently reliable results. As a calculation example for the real facilities, the motion response and the drift force of the vertical cylinder type ocean monitoring buoy with 2.6 m diameter and 3,77 m draft are calculated and discussed. The obtained results of motion response can be used to determine the shape and dimension of the buoy to reduce the motion response, and other data such as the effect of motion reduction due to a damper can be predictable through these motion calculations. Also, the calculation results of drift force can be used in the design procedure of mooring system to predict the maximum wave load acting on the mooring system. The present method has, in principle, no restriction in the application to the arbitrary shape facilities. So, this method can be a robust tool for the design, installation, and operation of various kinds of the floating-type ocean monitoring facilities.

• Conservation Science in Museum
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• v.17
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• pp.55-68
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• 2016

Conservation and research efforts are currently underway at the National Museum of Korea on a series of artifacts excavated from Tomb No. 9 at Seogam-ri nearly a century ago by an archaeology team from the institution's colonialera predecessor. Among these objects are a number of bronze and gilt bronze buttons. The present study explores the production techniques used in making such buttons. Both bronze and gilt bronze buttons are hemispherical in shape and are similar in external appearance. However, their shanks differ significantly in size and cross-sectional shape, some with a round cross-section while others are square. The buttons, first sorted into two groups by each type of shank, were further subdivided by size. Analysis of round shanks has found variations in design and location as well as in shank thickness. In addition, the location where round shanks attach to buttons are usually irregular in surface. Square shanks, on the other hand, are more uniform in design and location. The shanks are typically located on the backs of buttons and attach to a groove which ostensibly serves to mark the correct position. X-ray imaging has revealed that round shanks have thick borders made from metal rather than dirt or other material. The buttons themselves appear to been cast using lost-wax technique. The ways in which each shank attaches to its respective button varies based on its cross-sectional shape.