• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6511-6516
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• 2012

Recently, we reported that an ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. However, the detailed mechanisms were not fully explored. Here, we demonstrate another aspect of the activity of I. nertschinskia in breast cancer cells. We compared the response to an ethanol extract of I. nertschinskia in two different human breast cancer cell lines, Hs578Tand MDA-MB231, respectively with relatively low and high AKT1/2 activity by trypan blue exclusion assay and FACS analysis. Knockdown of endogenous AKT1 or AKT2 in breast cancer cells by RNA interference determined the sensitivity to I. nertschinskia ethanol extract compared to control cells. The I. nertschinskia ethanol extract induced cell death in a manner that depended on the level of phosphorylated AKT1/2 protein and was associated with a significant increase in the sub-G1 cell population, indicative of apoptosis. Our results indicate that an ethanol extract of I. nertschinskia differentially induces cell death in breast cancer cells depending on their level of phosphorylated AKT1/2.

• Research in Plant Disease
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• v.9 no.3
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• pp.125-127
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• 2003

Rust disease was occurred on Iris in several farmer's fields fields located in Hoehwa-myon, Goseong-gun, Gyeong-nam province in Korea. The typical symptoms of the disease appeared first as small, yellowish spot on leaves. The spots then turn brown, their edges rised slightly, and powdery mass of yellowish brown spores revealed on the lesions. Severely infected leaves were blighted and eventually died. Urediniospores were yellowish brown to reddish brown in color, obobid to obovoid in shape and 22~30 ${\times}$ 21~25${\mu}m$m in size. Teliospores were chestnet-brown in color, oblong or clavate in shape and 32~54 ${\times}$ 12~22${\mu}m$ in size. The causal fungus was identified as Puccinia iridis, based on morphological characteristics. This is the first report on the rust of Iris nertschinskia by Puccinia iridis in Korea.

• Korean Journal of Environment and Ecology
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• v.22 no.6
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• pp.640-647
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• 2008

This study was carried out to select the utilizable plants for the slope revegetation in dams, lakes and streams. In this study, 4 Iris species were investigated for their survival and growth characteristics in the complete and partly flooding condition with various flooding periods. The results are as followings; Iris pseudoacorus showed the highest flooding tolerance based on survival rate and growth condition. Iris ensata var. spotanea, I. setosa and I. nertschinskia followed after Iris pseudoacorus in the order of flooding tolerance. When I. pseudoacorus were completely submerged in the water, their growth was disturbed but they did not die up to 120 days. On the other hand, I. nertschinskia, I. setosa and I. ensata var. spotanea showed a significant growth disturbance in the complete water submergence and died in 90 days. This explained that they were relatively strong in flooding tolerance. They are considered to grow back to normal with some changes of water level.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.4
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• pp.74-84
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• 1999

The purpose of this study was to figure out the utilization of native plants for highway landscape. The field and nursery experiments were conducted from January of 1996 to December of 1998 on the Chonju Arboretum and Honam highway in Korea Highway Corporation. We have investigated thirty species of native plants and came to a conclusion that Chrysanthemum zawadskii var. latilobum, Aster koraiensis, Iris nertschinskia, Fennisetum alopecuroides, Viola kapsanensis are very suitable for highway landscaping. And, if the problems raised during this investigation are resolved, it is clear that Polygonatum odoratum var. plurijlorum, Belamcanda chinensis, Allium thunbergii, Ophiopogon japonicus, Dianthus superbus var. longicalycinus, Disporum sessile, Lilium tigrinum, Caryopteris incana, Aquilegia flabellata var. pumila, Pulsatilla koreana, Aster spathulifolius etc. can be useful plants at highway landscaping.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.104-111
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• 1994

The marsh vegetation in the vicinity of Tumunsan, Tokyusan National Park, Chollapuk-do, Korea was analysed in order to find out the vegetational characteristics. The total number of vascular plants surveyed were summarized as 39 familieis, 77 genera, 74 species, 18 varieties and 1 form. The ten species such as Juncus effusus var. decipiens, Carex leiorhyncha, Lysimachia vulgaris var. davurica, Sirpus triqueter, Scirpus karuizawensis, Caltha palustris var. membranacea, Carex doniana, Carex humilis, Vicia venosa, and Disporum smilacinum showed the Importance Value more than 10 % in herbaceous marsh vegetation. The species such as Heloniopsis orientalis, Astilbe chinensis var. davidii, Trignotis peduncularis, Lycopus ramosissimus var. japonicus, Iris nertschinskia and Hosta capitata were distributed at very limited or wide spread localities in the marsh. The marsh vegetation in this area is one of the rare and very distinct natural resources in Tokyusan National Park and the proper Action Plan of Management is strongly needed because the projected developing works in this site will act seriously in marsh vegetational changes in the future.

• Korean Journal of Agricultural Science
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• v.17 no.1
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• pp.9-17
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• 1990

Erwinia carotovora subsp. carotovora, Pseudomonas viridiflava and Pseudomonas marginalis pv. marginalis were tested for their pathogenicity to 35 kinds of domestic flowering plants. Among them, the following domestic flowering plants showed clear symptoms. 1. Erwinia carotovora subsp. carotovora : (Needle inoculation). Carnation(Dianthus catgophylius L.), madagascar periwinkle(Vinca rosea L.), flower gentle(Amaranthus tricolor L.), snapdragon(Antirrhinum majus L.), chrysanthemum(Chrysanthemum morifolium Ram.), mexiacan ageratum(Ageratum houstonianum Mill), china aster(Callistephus chinensis), youth and old age(Zinnia elegans Jacq.), common nasturtium(Tropaeorum majus L.), scarlet sage(Salvia splendens F.), dahlia(Dahia hybrida), pot marigold(Calendula officinalis L.), begonia treevine(Cissus dicolor Blume), cosmos(Cosmos bipinnatus Cav.), globe amaranth(Gomphrena globosa L.), black eyed susan(Thumbergia alata Bojer), common gypsophila(Gypsophila elegans Bieb.), ghent gladiolus(Gladiolus gandavensis Van.), indian shot(Canna orchiodes Bailey), iris(Iris nertschinskia Lodd), cyclamen primula(Dodecatheon meadia L.), scarlet kafir lily(Clivia miniata Regel.), flowering cabbage(Brassica oleracea L.). (Spray inoculation). Carnation, madagascar periwinkle, flower gentle, snapdragon, common nasturtium, ghent gladiolus, indian shot, cyclamen primula. 2. Pseudomonas viridiflava : (Needle inoculation). Carnation, madagascar periwinkle, snapdragon, chrysanthemum, cockscomb, mexican ageratum, china aster, common nasturtium, common petunia(Petunia hybrida Vilm), pot marigold, begonia treevine, cosmos, black eyed susan, common gypsophila, ghent gladiolus, indian shot, cyclamen primula, scarlet kafir-lily, flowering cabbage. (Spray inoculation). Common nasturtium, ghent gladiolus, indian shot, cyclamen primula. 3. Pseudomonas marginalis pv. marginalis : (Needle inoculation). Carnation, madagascar periwinkle, flower gentle, snapdragon, cockscomb, mexican ageratum, youth and old age, common nasturitium, common petunia, sweet william(Dianthus barbatus L.), pot marigold, begonia treevine, cosmos, common gypsophila, ghent gladiolus, indian shot, iris, cyclamen primula, scarlet kafir-Lily, flowering cabbage. (Spray inoculation). Common nasturtium, ghent gladiolus, indian shot, cyclamen primula.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.43-49
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• 1999

To develope wild flower collected from Chollabuk-do area resource plant species were investigated from May 1996 to April 1997. This study was focussed to make the basic data for better quality of breeding species from wild flower species and to keep proper gene pool for development with competitive power internationally in future. There was 131 species blooming in spring season(34.7%), 219 species in summer(58.1%), 25 species in autumn(6.6%) and 2 species in winter(0.5%), respectively. Flower color of all the plants was 26 kinds in diverse, among them, white flowers were occupied dominantly of 28.9%, yellow flowers, 20.7%, violet flowers, 16.4% and red purple flowers, 7.9%, respectively. Transplanted wild flower plants at the garden in the study area frequently were Chelidonium majus var. asiaticum, Hemerocallis fulva, Iris nertschinskia, Rhododendron yedoense var. poukhanense, Rhododendron mucronulatum, Pulsatilla koreana, Chrysanthemum zawadskii var. latilobum, Taraxacum platycarpum, Chrysanthemum boreale, Liriope platyphylla and Lycoris radiata. Twenty-six species of wild flowers such as Cymbidium goeringii, H. fulva, L. radiata, C. zawadskii var. latilobum, etc. were collected from their habitat and being selling at the market or road side. Total 343 taxa of wild flowers to prevent carrying out to foreign countries and to improve better quality were selected and recommended in this study, for example, they were 41 taxa of Compositae plant, 25 of Rosaceae, 20 of Liliaceae and 18 of Ranunculaceae in order. Acorus calamus var. angustatus, Cypripedium japonicum, C. macranthum, Calanthe discolor, Dicentra spectabilis, Ilex cornuta, Stewartia koreana, Abeliophyllum distichum, Forsythia saxatilis and Campanula glomerata var. dahurica should be protected as wild flowers because their numbers are decreasing remarkably, belong to rare species, being at a crisis to extinct species and important plants for research materials.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.173-182
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• 2007

The purpose of this study was to compared the early growth pattern, stem, leaf and root weight, DM yield, flowering characteristics and preference for selection of turf type grasses and wild flower species in mixtures combination for wildflower pasture. The experimental design included five species of turf grasses and 11 species of wildflower : turf type grass species{Kentucky bluegrass(Midnight), tall fescue(Millennium), perennial ryegrass(Palmer III), redtop(Barricuda) and creeping bentgrass(Crenshaw)}, wildflower species {Chrysanthemum leucanthemum L. Coreopsis lanceolata L., Rudbeckia bicolor Nutt.., Dianthus chinensis L., Chrysanthemum lindicum L., Lotus corniculatus var. Japonicus Regel, Veronica linariaefolia Pallas, Aster koraiensis Nakai., Chrysanthemum zanadskii var. latilobum (Maxim.) Kitamura, Lythrum anceps Makino and Iris pallassi var. chinensis Lodd}. The field trials were carried out on the experimental pasture plots of Chungnam National University from January 2005 to December 2006. The appropriate species of turf grass which have a mixture possibility with wildflower are thought to be Kentucky bluegrass. Compared with Kentucky bluegrass, on the other hand, species such as Chrysanthemum leucanthemum L., Lotus corniculatus var. Japonicus Regel, Chrysanthemum zanadskii var. latilobum Maxim.) Kitamura and Lythrum anceps Makino have shown advantageous aspects of fast early growth pattern and highly weights of stem, leaf and root. Futhermore, these wild flowers were in harmony and no overlap on flower color, flowering periods and flowering longevity, and highly preference by Korean native goats. For the establishment of wildflower pasture in Korea, therefore, it is important to select the appropriate species of turf glasses, which have a weak competition against wild flowers, and then to make mixture combination of those turf glasses with the various wild flowers which have a strong competition against turf glasses but have a different anthesis characteristics among themselves.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.2
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• pp.89-98
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• 2008

The purpose of this study was to suggest the basic data for establishment of wildflower pastures. The experimental design includes two treatments: Native wildflower pasture (NWP, Kentucky bluegrass + native wildflower 8 species) and Introduced wildflower pasture (IWP, Kentucky bluegass + introduced wildflower 24 species). The field trials were carried out on the experimental pastures plots at Chungnam National University throughout from Sep. 2006 to Dec. 2007. The results obtained are as fellows: In terms of the flower color in the wildflower pastures, the IWP was found more various than the NWP. But such problems as seasonal distribution and continuity were raised and moreover the time of flowering depended mainly upon the spring season. Even though the flower color in the NWP did not show up its variousness, seasonal distribution and continuity were found comparatively satisfactory. To perennially maintain the botanical composition, the variousness of wildflower species are found important after examining locally the beginning rearing and flowering characteristics of wildflowers as well as the basic turfgrass. Moreover, when the wildflower pastures was constructed in autumn, since most of the introduced wildflower were annuals, it maintained various botanical composition before wintering, but after wintering the botanical composition were found simplified due to the winter tolerance. The NWP did not show any problems in the winter tolerance but it could not make the botanical composition diversified. Accordingly, to maintain the wildflower pasture for many years, diversifying wildflower species based upon perennials including annuals seems to be good.