• Journal of the Korean Chemical Society
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• v.30 no.1
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• pp.9-18
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• 1986

MNDO and STO-3G calculations were performed to determine relative stabilities of rotamers for ${\alpha}$-substituted acetones, $CH_2XCOCH_3$, X = F, Cl, OH, SH, and $NH_2$. It was found that rotamers corresponding to gauche forms are preferred for all the ${\alpha}$-substituents except for X = F and NH$_2$, for which the cis forms were the preferred ones. The stability of gauche form was dictated by the stabilizing two-orbital-two-electron interaction ${\sigma}_{cx}$-${\pi}_{co}^*$, operating uniquely in the gauche form due to the substantial vicinal overlap and energy gap narrowing between ${\sigma}_{cx}$ and ${\pi}_{co}^*$ orbitals. The energy gap narrowing was caused by the lowering of ${\pi}_{co}^*$ level due to the hyperconjugative ${\sigma}_{cx}^*$-${\pi}_{co}^*$ interactions; the red shift in the n-${\pi}^*$ transition was another effect of the relatively large ${\sigma}_{cx}^*$-${\pi}_{co}^*$ splitting. Various ${\sigma}-{\pi}$ interactions in the gauche form were found to be stronger in the third-row hetero atom system, X = Cl and SH. Interactions between nonbonding orbital on N, $n_N$ and vicinal C-C ${\sigma}$ bond were shown to be stronger in the trans than in the cis orientation.

• Journal of Life Science
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• v.25 no.6
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• pp.631-637
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• 2015

Streptavidin, a protein produced by Streptomyces avidinii, strongly binds up to four molecules of vitamin H, d-biotin exhibiting the dissociation constant of about 10⁻¹⁵ M. This strong binding affinity has been applied for detection and characterization of numerous biological molecules suggesting expression and purification of functional streptavidin should be very useful for the application of this streptavidin-biotin interaction. To express a soluble streptavidin in Escherichia coli, We synthesized streptavidin genes and cloned into pET-22b plasmid, which uses T7 RNA polymerase/T7 promoter expression systems containing pelB leader for secretion into periplasmic space and six polyhistidine tags at C-terminus for purification of expressed proteins. Although streptavidin is toxic to Escherichia coli due to strong biotin binding property, streptavidin was expressed very sufficiently in a range of 10-20 mg/ml. In SDS-PAGE, the size of purified protein was shown as 17 kDa in denatured condition (boiling) and 68 kDa in native condition (without boiling) suggesting tetramerization of monomeric subunit by non-covalent association. Further analysis by size-exclusion chromatography supported streptavidin’s tetrameric structure as well. In addition, soluble streptavidin detected biotinylated proteins in westernblot indicating its functional activity to biotin. Taken these results together, it concluded that our simple expression system was able to show high yield, homotetrameric formation and biotin binding activity analogous to natural streptavidin.

• Journal of Life Science
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• v.26 no.9
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• pp.1027-1032
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• 2016

Antigen is substance causing disease derived from pathogen. Living organism has the immune system in terms of defense mechanism against antigen. Antigen is processed through several pathways such as phagocytosis, antibody action, complement activation, and cytotoxins by NK or cytotoxic T lymphocyte via MHC molecule. Lymph node (LN) is comprised of the complicated 3 dimensional network and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in T zone for interaction with T cells. FRC produces the extra cellular matrix (ECM) into LN for ECM reorganization against pathogen infections and secretes homing chemokines. However, it has not so much been known about the involvement of the antigen process of FRC. The present report is for the function of FRC on antigen process. For this, FRC was positioned with several infected situations such as co-culture with macrophage, T cell, lipopolysaccharide (LPS) and TNFα stimulation. When co-culture between FRC with macrophage and T cells was performed, morphological change of FRC was observed and empty space between FRCs was made by morphological change. The matrix metallo-proteinase (MMP) activity was up-regulated by Y27632 and T cells onto FRC. Furthermore, inflammatory cytokine, TNFα regulated the expression of adhesion molecules and MHC I antigen transporter in FRC by gene chip assay. NO production was elevated by FRC monolayer co-cultured with macrophage stimulated by LPS. GFP antigen was up-taken by macrophage co-cultured with FRC. Collectively, it suggests that FRC assists of the facilitation of antigen process and LN stroma is implicated into antigen process pathway.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.7
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• pp.630-642
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• 2013

To transmit vital signal stream of mobile patients remotely, it requires mobility of patient and watcher, sensing function of patient's abnormal symptom and self-organizing service binding of related computing resources. In the existing relative researches, the vital signal stream is transmitted as a centralized approach which exposure the single point of failure itself and incur data traffic to central server although it is localized service. Self-organizing middleware platform based on heterogenous overlay network is a middleware platform which can transmit real-time data from sensor device(including vital signal measure devices) to Smartphone, TV, PC and external system through overlay network applied self-organizing mechanism. It can transmit and save vital signal stream from sensor device autonomically without arbitration of management server and several receiving devices can simultaneously receive and display through interaction of nodes in real-time.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.3
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• pp.119-126
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• 2019

For this purpose, research was made to look into the style, material, organization, and legal as well as institutional system of the bonsai industry in the three countries. In order to understand what significance it has and how it has changed historically, related literature was surveyed and questionnaires were distributed to the concerned people to collect the data needed for them to plan bonsai industry. The results of the survey are as follows. Analysis of awareness of bonsai culture, respondents replied that the motive and purpose of bonsai is because they like flower trees, and the technical information about bonsai is usually obtained by visits to bonsai gardens. The analysis revealed that respondents usually join more than one bonsai club and are active more than once a month, which means longer than 4 hours each month, and their activities are more frequently in the metropolitan area. From the analysis of preference for bonsai culture, it was made known that the favorite bonsai size is less than middle one(20~50cm), the characteristics of the favorite species are beauty. A research and analysis of bonsai-related technical education found that bonsai is trimmed manually in general, bonsai experts are usually kept in contact with, and the bonsai technique is learned through interaction with the experts.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.2
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• pp.63-71
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• 2002

This paper examines the ASCE first generation benchmark problem for a seismically excited cable-stayed bridge, and proposes a new semi-active control strategy focusing on inclusion of effects of control-structure interaction. This benchmark problem focuses on a cable-stayed bridge in Cope Girardeau, Missouri, USA, for which construction is expected to be completed in 2003. Seismic considerations were strongly considered in the design of this bridge due to the location of the bridge in the New Madrid seismic zone and its critical role as a principal crossing of the Mississippi River. In this paper, magnetorheological(MR) fluid dampers are proposed as the supplemental damping devices, and a clipped-optimal control algorithm is employed. Several types of dynamic models for MR fluid dampers, such as a Bingham model, a Bouc-Wen model, and a modified Bouc-Wen model, are considered, which are obtained from data based on experimental results for full-scale dampers. Because the MR fluid damper is a controllable energy-dissipation device that cannot add mechanical energy to the structural system, the proposed control strategy is fail-safe in that bounded-input, bounded-output stability of the controlled structure is guaranteed. Numerical simulation results show that the performance of the proposed semi-active control strategy using MR fluid dampers is quite effective.

• Physical Therapy Korea
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• v.14 no.2
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• pp.44-52
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• 2007

This study was carried out to compare the muscle activities of the shoulder stabilizers between persons with and without winging scapular during push-up plus exercise (adds the scapular protraction to the general push-up exercise). For this study, eleven males with winging scapular and eleven healthy males were recruited. Surface electromyographic (EMG) activity was recorded from the serratus anterior, upper trapezius, lower trapezius, infraspinatus, and pectoralis major while the subjects performed the push-up plus. Each push-up plus was subdivided into three phases according to the elbow position which was measured using the 3-D motion analysis system: elbow flexion (EF), elbow extension (EE), and shoulder protraction phases (SP). Two-way repeated measure ANOVA (phase ${\times}$ group) were used for statistical analysis. There was significant phase by group interaction only on the EMG composition ratio of the serratus anterior (p>.05). The EMG composition ratio of the serratus anterior was significantly higher in SP than in either EF or EE however, it was not different between winging scapular and normal groups. For both groups, the EMG composition ratio of upper trapezius, lower trapezius, and pectoralis major was significantly different across the phases of push-up plus, but the infraspinatus EMG composition ratio was not. For both groups, in EF and EE phases, the EMG composition ratio of both pectoralis major and serratus anterior were relatively higher than that of other muscles. However, in both groups, the EMG composition ratio of the serratus anterior became much more predominant than that of the pectoralis major. In addition, infraspinatus activated greater than pectoralis major. These results showed that the push-up plus exercise is effective to selectively strengthen the serratus anterior for both individuals with and without winging scapular, but not equally effective for other shoulder stabilizers.

• Journal of the Economic Geographical Society of Korea
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• v.1 no.1
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• pp.57-74
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• 1998

Recently. the interest in Physical distribution has increased in economic geography This paper aims to review and prospect the internatonal physical distribution studies of international trade and international freight flows. And it has examined the changes in methodology of international physical distribution. Author has defined international physical distribution as the physical distribution of international trade and international freight flow The results are as follows : (1) As the methodology of international physical distribution studies developes, the physical distribution should be recognized not only as transportation phenomenon but also as total logistics which is understood as the combination of production and consumption including information flow. (2) The studies about model of international physical distribution flow ill need to study regional relations including urban, in the future. And economic geography and location theory adding culture and behaviour elements will be restructured, because not only state but also multinational corporation appear as trade subject in global economy. (3) The studies for the relation of habor and its hinterland in Physical distribution need to analyze the factors causing habor use with decision-making behaviour of corporation. (4) After oil crisis. as air freight is important, the studies for hinterland and foreland of freight centering international airport are needed because of necessity of light and small freight. flexible Production system, free trade, and high speed freight transportation of freshing food and flowers and so forth.

• Korean Journal of Social Welfare
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• v.38
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• pp.284-308
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• 1999

This study is about the nationwide research concerning the degree and trends of marital conflict and instability. The research samples are 1,217 married. As the result of the research, the most conflict able issues in Korean couples are on the husband-wife relations, such as habitual differences, sharing in houseworks, conflicts in the role expectation, and the communication problems. In this study, We devide the marital relationships into 4 types (A, B, C, and D) in the view of the satisfaction & instability of marriage. First, the marriage with unsatisfaction and instability (A) was portioned 8.3%, and the satisfactory & stable type (D) was portioned 68.4%. It seems the marital relationships in Korea are relatively stable. However, the portion of type B-unsatisfactory, but stable couples-is 17.1% and the type C-satisfactory, but unstable marriage 6.2%. The result implies that the indication of marital instability should consider not only the divorce rate, but also the quality of marital relationships. In other words, the intervention for the conflict couples yet not divorce is needed. The differences of conflict areas among the relational types is not so much, but the degrees on conflicts are very serious. Especially, in the areas of marital conflict, love issues and personal value system affect the division of relational types of marriage most. It means the interaction factors are the most important variables about maintaining the marital relations with stable and satisfaction.

• Journal of Intelligence and Information Systems
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• v.12 no.2
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• pp.67-81
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• 2006

While the existing Web focuses on the interface with human users based on HTML, the next generation Web will focus on the interaction among software agents by using XML and XML-based standards and technologies. The inference engine, which will serve as brains of software agents in the next generation Web, should thoroughly understand the Semantic Web, the standard language of the next generation Web. As abasis for the service, the W3C (World Wide Web Consortium) has recommended SWRL (Semantic Web Rule Language) which had been made by compounding OWL (Web Ontology Language) and RuleML (Rule Markup Language). In this research, we develop a backward chaining inference engine SMART-B (SeMantic web Agent Reasoning Tools -Backward chaining inference engine), which uses SWRL and OWL to represent rules and facts respectively. We analyze the requirements for the SWRL-based backward chaining inference and design analgorithm for the backward chaining inference which reflects the traditional backward chaining inference algorithm and the requirements of the next generation Semantic Web. We also implement the backward chaining inference engine and the administrative tools for fact and rule bases into Java components to insure the independence and portability among different platforms under the environment of Ubiquitous Computing.