• Design & Manufacturing
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• 제2권5호
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• pp.5-10
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• 2008

Recently, ultra precision and light-weight micro products are needed in various industries. Injection molding products with metal insert material is often satisfied with light-weight and precision simultaneously. The researches on macro-size insert deformation have been performed but, a research on micro-size insert is meager. In this paper, the injection molding product with $300{\mu}m$ thin foil insert is designed and insert injection molding process is performed. Finally, the deformation of thin foil insert is analyzed according to insert feature and gate length.

• 한국응용곤충학회지
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• 제54권2호
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• pp.91-97
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• 2015

담배가루이(Bemisia tabaci)는 외래해충으로 바이러스벡터로 작용하여, 토마토의 토마토황하잎말림병바이러스(TYLCV)를 비롯한 약 100여종의 바이러스를 매개하는 중요한 해충이다. 본 연구에서는 VIGS vector를 이용하여 담배가루이 방제를 위한 target 유전자들을 선발하기 위해 gateway system을 이용한 담배가루이 cDNA library 제작을 시도하였다. 첫 번째 방법으로 oligo d(T) primer를 사용하였을 때, 평균 약 1 kb의 insert와 $1.4{\times}10^4cfu$의 titer를 확인하였다. 그러나 insert size가 너무 커서 적절하지 않았다. 두 번째 방법으로 attB-N25 random primer를 이용하고, sonication을 6초 실시하여 다시 진행하였다. 그러나 확인되는 insert size는 다소 컸고, 몇몇은 insert가 너무 작아서 밴드가 확인 되지않았으며, $1.04{\times}10^54cfu$의 titer를 확인할 수 있었다. 세 번째 방법으로는 oligo d(T) primer를 이용하였고, sonication을 2초 실시하였다. 그 결과 300 bp~600 bp size의 insert가 확인되었으나, electro transformation을 사용한 첫번째, 두번째 방법에 비해 heat shock transformation을 사용하여 titer가 $5.2{\times}10^24cfu$로 매우 낮은 것을 확인 할 수 있었다. 결과적으로 cDNA library를 만들 때 먼저 random primer를 사용하여 First strand를 합성하여 poly A를 제거하고, 다음으로 sonication을 1초 실시하여 300~700 bp정도의 적절한 size의 insert를 생성하고, 마지막으로 electro-transformation을 실시하여 transformation 효율을 높인다면 VIGS vector에 적합한 cDNA library를 만들 수 있을 것으로 사료 된다.

• Journal of Mechanical Science and Technology
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• 제16권7호
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• pp.1019-1028
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• 2002

The instability of two-phase loop thermosyphons was investigated experimentally and analytically. Three orifice type inserts were used to study the effect of change in the pressure drop in the flow channel of the TLT on the flow instability and temperature fluctuation. It is observed that a decrease in the size of the orifice insert from 3.7 mm (no insert) to 0.71 mm drastically reduced the fluctuation of the temperature, especially at the evaporator section of the TLT. With the orifice type insert of 0.71 mm for the TLT, the overall temperature fluctuation was almost completely eliminated, especially at higher power input to the TLT The analysis based on the Kelvin-Helmholtz instability theory seems to predict reasonable well the loop stability state of the TLT with experimentally determined constant factors.

• 설비공학논문집
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• 제14권5호
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• pp.408-414
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• 2002

The instability of two-phase loop thermosyphons (TLTs) was investigated experimentally. Three orifice type inserts were used to study the effect of change in the pressure drop in the flow channel of the TLT on the flow instability and temperature fluctuation. It is observed that a decrease in the size of the orifice insert from 3.7mm (no insert) to 0.71mm drastically reduced the fluctuation of the temperature, especially at the evaporator section of the TLT With the orifice type insert of 0.71 mm for the TLT, the overall temperature fluctuation was almost completely eliminated, especially at higher power input to the TLT.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 춘계학술대회 논문집
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• pp.535-538
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• 2008

Insert-injection molding can inject two different materials or two colors in the same mold and process. If this injection process use, product has ability because the base part maintain strength and specified part can inject soft-material. It makes the cost down by single operation automatically for saving wages. In this paper, we designed double-injection mold for automobile remote control to inject secondary using this part as insert after inject external appearance of product. CAE analysis was progressed gate location and runner size as variable and analysis result is reflected in mold design process. As a result, it could solved badness that is generated at the conventional mold. Additionally, cost is downed by reducing loss of runner as well as could omit painting process because surface of finished product is improved through new mold.

• Design & Manufacturing
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• 제11권3호
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• pp.29-34
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• 2017

Increased demand for parts with micro-pattern structure made of metals, ceramics, and composites in various fields such as medical ultrasonic sensors, CT collimators, and ultra-small actuator parts. Micro powder injection molding (PIM) is a technology for manufacturing micro size, high volume, complex, precision, net-shape components from either metal or ceramic powder. In the present study, a standard mold with a variable insert core capable of producing various micro patterns was investigated. An injection molding test was performed on a standard mold using a line type micro-pattern core having an aspect ratio of 2, a slenderness ratio of 70, a pattern size of $200{\mu}m$, and a pattern spacing of $150{\mu}m$. During the filling process, the deformation of the mold with large aspect ratio and slenderness ratio was analyzed by the experiment and the numerical simulation according to the position of the gate. We proposed a mold structure that minimizes mold deformation by gate modification and enables uniform pattern filling behavior.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.565-570
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• 2011

본 연구는 파인 블랭킹(fine blanking) 프레스로부터 기능면으로 사용되는 100% 매끄러운 전단면을 얻는 파인 블랭킹 공정에서 다이 롤(die roll) 크기를 최소화하기 위한 목적으로 실험을 통해 다이 챔퍼(die chamfer) 형상에 따라 성형되는 제품의 다이 롤 크기 변화를 검토한 것이다. 다이 챔퍼가 다른 여러 가지 다이 편(die insert)을 제작하여 파인 블랭킹 실험을 실시한 후 다이 챔퍼 형상에 따른 다이 롤 크기를 측정 분석하여 두께 방향 다이 롤 크기의 경향을 파악하였다. 이 연구 결과는 파인 블랭킹 판재 성형에서 두께 방향 다이 롤 크기를 최소화하기 위한 다이챔퍼 설계에 유용하게 적용될 것으로 판단된다.

• 한국터널지하공간학회 논문집
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• 제21권1호
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• pp.49-59
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• 2019

본 연구는 로터리드럼커터에 적용된 두 종류의 코니컬 픽커터에 대한 현장시험을 통해 시험 전과 후의 육안조사와 중량감소 및 마모부피를 측정하여 코니컬 픽커터의 내구성을 비교 분석하였다. 육안조사를 통해 슬림 타입 코니컬 픽커터에서만 총 9개의 삽입재 손실이 나타남을 확인하였고 삽입재 보호를 위해 두부의 두께가 중요함을 알 수 있었다. 시험 전과 후의 픽커터의 중량손실과 마모부피는 헤비타입이 슬림타입에 비해 반이하로 작게 나타나 경암반에서 헤비타입이 더 유용함을 확인할 수 있었다. 픽커터의 마모손실을 판단할 때, 중량측정과 마모부피 측정결과의 상호 비교는 재료의 단위중량과 굴착에 의한 스폴링에 의해 차이가 나타날 수 있으므로 주의해야 할 것으로 판단된다.

• Genomics & Informatics
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• 제4권2호
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• pp.80-86
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• 2006

It is clear that the construction of large insert DNA libraries is important for map-based gene cloning, the assembly of physical maps, and simple screening for specific genomic sequences. The bacterial artificial chromosome (BAC) system is likely to be an important tool for map-based cloning of genes since BAC libraries can be constructed simply and analyzed more efficiently than yeast artificial chromosome (YAC) libraries. BACs have significantly expanded the size of fragments from eukaryotic genomes that can be cloned in Escherichia coli as plasmid molecules. To facilitate the isolation of molecular-biologically important genes in Ashbya gossypii, we constructed Ashbya chromosome-specific BAC libraries using pBeloBAC11 and pBACwich vectors with an average insert size of 100 kb, which is equivalent to 19.8X genomic coverage. pBACwich was developed to streamline map-based cloning by providing a tool to integrate large DNA fragments into specific sites in chromosomes. These chromosome-specific libraries have provided a useful tool for the further characterization of the Ashbya genome including positional cloning and genome sequencing.

• Design & Manufacturing
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• 제14권1호
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• pp.15-21
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• 2020

Smart systems in different application areas such as automotive, medical and consumer electronics require a novel manufacturing method of electronic, optical and mechanical functions into products. Traditional methods including mechanical assembly, bonding of plastic and electronic circuit cause the problems in large size of products and complicated manufacturing processes. In this study, thermoforming and film insert molding were applied to fabricate a dome shaped plastic part embedded with electronic circuits. The deformation of patterns printed on PET film was predicted by thermoforming simulation using T-SIM, and the results were compared with those by experiment. In order to decrease spring-back after thermoforming, the Taguchi method of design of experiment was used. Through ANOVA analysis, it was found that mold temperature was the most dominant parameter for spring-back. By using flow analysis, gate design was performed to decrease injection pressure. During film insert molding, the wash-out of ink printed on film occurred for Polycarbonate. When the resin was changed to PMMA, the wash-out disappeared due to low melt temperature.