• Biotechnology and Bioprocess Engineering:BBE
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• v.1 no.1
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• pp.22-25
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• 1996

An Exponetial feeding strategy has been frequently used in fed-batch fermentation of recombinant E. coli. In this feeding scheme, growth yield and initial cell concentration, which can be erroneously determined, are needed to calculate the feed rate for controlling specific growth rate at the set point. The effect of the incorrect growth yield and initial cell concentration on the control of the specific growth rate was theoretically analyzed. Insignificance of the correctness of those parameters for the control of the specific growth rate was shown theoretically and experimentally.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.2 s.233
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• pp.169-179
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• 2005

Nucleate boiling experiments on heating surface of constant wall temperature were performed using R113 for almost saturated pool boiling conditions. A microscale heater array and Wheatstone bridge circuits were used to maintain a constant wall temperature condition of heating surface and to measure the heat flow rate with high temporal and spatial resolutions. Bubble images during the bubble growth were taken as 5000 frames per second using a high-speed CCD camera synchronized with the heat flow rate measurements. The bubble growth behavior was analyzed using the new dimensionless parameters for each growth regions to permit comparisons with previous experimental results at the same scale. We found that the new dimensionless parameters can describe the whole growth region as initial and later (thermal) respectively. The comparisons showed good agreement in the initial and thermal growth regions. In the initial growth region including surface tension controlled, transition and inertia controlled regions as divided by Robinson and Judd, the bubble growth rate showed that the bubble radius was proportional to $t^{2/3}$ regardless of working fluids and heating conditions. And in the thermal growth region as also called asymptotic region, the bubble showed a growth rate that was proportional to $t^{1/5}$, also. Those growth rates were slower than the growth rates proposed in previous analytical analyses. The required heat flow rate for the volume change of the observed bubble was estimated to be larger than the heat flow rate measured at the wall. Heat, which is different from the instantaneous heat supplied through the heating wall, can be estimated as being transferred through the interface between bubble and liquid even with saturated pool condition. This phenomenon under a saturated pool condition needs to be analyzed and the data from this study can supply the good experimental data with the precise boundary condition (constant wall temperature).

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.2
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• pp.117-120
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• 2002

The effect of cell density on cell growth was investigated in a suspension batch culture of hybridoma cells. The specific growth rate was found to increase with increasing initial cell density and then to decrease with further increases in initial cell density. In order to quantitatively describe the dependence of specific growth rate on cell density, a kinetic model is proposed, which satisfactorily represents the experimental data.

• Microbiology and Biotechnology Letters
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• v.23 no.4
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• pp.479-484
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• 1995

The effect of initial cell concentration on the characteristics of ethanol fermentation was investigated in the batch fermentation of Saccharomyces cerevisiae ATCC 24858. The characteristics were investigated in the range of 60 to 230 g/l of the initial sugar concentrations and 0.5 to 85 g/l of the initial cell concentrations. When the initial cell concentrations were 27 g/l for 60 g/l of the initial sugar and 85 g/l for 230 g/l, the fermentation time required for the complete consumption of the initial sugar was one hour, respectively. The ethanol productivity increased with the initial cell concentration so that, in the case of 100 g/l of initial sugar, the productivity rose up to 55 g/l/hr at 55 g/l of the initial cell concentration. The specific growth rate decreased according to the increase in the initial biomass concentration and finally became zero at around 25 g/l of the cell concentration regardless of the initial sugar concentration. The specific ethanol production rate was constant as 1.02 g/l/hr up to 150 g/l of the initial sugar. However, the rates decreased sharply with the augmentation of concentration of the initial sugar above 160 g/l. The overall ethanol yield represented a constant value, 0.475 g/g irrespective of the initial cell and sugar concentrations. The overall biomass yietd showed a trend to diminish in values with the biomass and ultimately to reach zero more than 25 g/l of the initial cell concentration.

• Journal of Plant Biology
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• v.37 no.4
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• pp.397-402
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• 1994

Possible roles of polyamines in the inhibition of cell elongation in Ranunculus petioles were investigated. Exogenously apoplied polyamines greatly inhibited the auxin-induced petiole growth, while treatment of the tissue with $\alpha$-difluoromethylarginine, the inhibitor of putrescine biosynthesis, further enhanced the growth in the presence of IAA. Inhibitory effect of spermine can also be apparent for fusicoccin-induced elongation, but not for growth induced by a low pH. Spermine also suppressed the ethylene-enhanced growth in the presence of auxin. Using computer-based video digitizer system, the inhibitory effects of spermine on petiole growth were kinetically analyzed. Auxin-induced growth was characterized by an initial and transient growth with a highly elevated rate followed by a steady growth with a slightly reduced rate. Spermine treatment was found to shorten the duration of the initial phase of growth, and to reduce the rates of both the initial and steady growth as well. The latent period for auxin induction was not affected by spermine.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.250-253
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• 2000

We researched effects of growth on initial inoculum size, liquid volume, and medium feeding rate etc. Cell suspension inoculated at low cell concentrations showed a typical growth reduction, whereas root cultures showed an improvement in growth. In this paper, Hairy roots showed high growth rate at 0.4 % inoculum size and 100 mL liquid volume in 250 mL flask cultures.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.7
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• pp.1722-1729
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• 1994

At early stage of creep-fatigue crack growth tests, initial transient behavior which implies high crack growth rate has been generally observed by some researchers. Since the influence of the initial transient crack growth behavior on the remaining life of components is significant, cause of it should be further studied. In this study, characteristics of the initial transient behavior of 1Cr-1Mo-0.25V steel is studied experimentally by performing creep-fatigue crack growth tests at $538^{\circ}C$ in air under trapezoidal waveshapes. It is verified that the cause of the initial transient behavior is not high ${(C_t)}_{avg}$ values due to the small scale creep condition at the early stage of test, but oxidation-dominated crack growth mechanism during the transient period which is different from the creep-dominated crack growth mechanism in steady crack growth period.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.1302-1306
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• 2005

This study has Performed to develop rehabilitation model for determination of optimal rehabilitation time on the Cast Iron Pipes (CIP) with diameter less than 300 mm in water pipeline network and to analysis the sensitivity on the rehabilitation times of developed model. In the result from the application of the field, the renovation time was faster about 10 years than the replacement time. Especially, as the difference between rehabilitation and replacement time on E-CIP was about from 3 to 5 years, and it was thought that the replacement was effective on E-CIP. To sensitivity analysis, the discount rate of coefficient was fixed at 0.08, and the values of initial year break rate(N($t_o$) and growth rate coefficient(A) were adjusted in values of 0.0009, 0.0018, 0.0027, and 0.05, 0.10, 0.15 respectively. When the values of N($t_o$) and A was increased, the results from the time of rehabilitation and replacement was faster It was thought that N($t_o$), 0.018 was reliable values on the applied pipeline through the result of the study. In case of A, the values of A above 0.1 was thought to be Proper.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.05a
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• pp.133-135
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• 2012

The Fire growth rate(kW/s²) is significant impact on initial fire behavior in fire safety design of buildings. As a result of domestic existing combustibles, this study analyzed considering matters in techniques for calculating caloric values, and then made an investigation sheet. By utilizing written combustion sheets, the study could suggest a standard model at common houses and dense ones after getting caloric value information in dense ones. As a result, fire growth rate is experiment 1(0.01), experiment 2(0.0048), FDS(0.0072), MATSUYAMA equation(0.0144).

• Journal of Welding and Joining
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• v.14 no.5
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• pp.69-77
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• 1996

This study aims to analyze the S. I. F. K value upon Mode I cracks in a finite-width plate of varying thickness, which is expressed in terms of width ratio ($\omega$), thickness ratio ($\beta$) and non-dimensional crack length (λ) by using the 2-dimensional finite element method. Then, by comparing the effectiveness of the results obtained by the two finite element methods, it is seen that the 2-dimensional finite element method can be used in order to analyse the S. I. F. K values upon a various thickness model. A model is developed in order to analyze the effects of initial residual stress upon the fatigue crack growth behavior in various thickness welded specimens. In this model, crack growth rate da/dN appears to be come small as the thickness ratio with the same ΔK is increased. Also, in the initial step, as ΔK is increased with crack growth rate is decreased and then increased because the repeated compressive residual stress retards crack growth rate.