Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Kinetic Analysis of the Effect of Cell Density on Hybridoma Cell Growth in Batch Culture

  • Lee, Eun-Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of cell density on cell growth was investigated in a suspension batch culture of hybridoma cells. The specific growth rate was found to increase with increasing initial cell density and then to decrease with further increases in initial cell density. In order to quantitatively describe the dependence of specific growth rate on cell density, a kinetic model is proposed, which satisfactorily represents the experimental data.

Keywords

References

  1. Trends Biotechnol. v.15 Overcoming obstacles to monoclonal antibody product development and approval Stein, K.E. https://doi.org/10.1016/S0167-7799(96)10075-5
  2. Trends Biotechnol. v.6 Animal cells in culture: moving into the exponential phase Spier, R.E. https://doi.org/10.1016/0167-7799(88)90106-0
  3. Biotechnol. Bioeng. v.32 A model for density dependent growth of anchorage-dependent mammalian cells Frame, K.K.;W.S. Hu https://doi.org/10.1002/bit.260320813
  4. Animal Cell Culture: A Practical Approach Freshney, R.
  5. Hybridoma v.8 Effect of serum concentration on hybridoma cell growth and monoclonal antibody production at various initial cell densities Lee, G.M.;T.K. Huard;B.O. Palsson https://doi.org/10.1089/hyb.1989.8.369
  6. Bio/Technology v.6 Catabolic control of mammalian cell culture Glacken, M.W. https://doi.org/10.1038/nbt0988-1041
  7. Biotechnol. Bioeng. v.48 Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture Banik, G.G.;C.A. Heath https://doi.org/10.1002/bit.260480315
  8. Biotechnol. Prog. v.12 Analysis of nutritional factors and physical conditions affecting growth and monoclonal antibody production of the hybridoma KB265 cell line Sanfeliu, A.;J.J. Cairo;C. Casas;C. Sola;F. Godia https://doi.org/10.1021/bp950078x
  9. J. Cell. Physiol. v.112 The role of vitamin E in cellular energy metabolism in cultured andrenocortical cells Hornsby, P.J. https://doi.org/10.1002/jcp.1041120208
  10. J. Biotechnol. v.46 The importance of ammonia in mammalian cell culture Schneider, M.;I.W. Marison;U. von Stockar https://doi.org/10.1016/0168-1656(95)00196-4
  11. Enzyme Microb. Technol. v.20 Batch cultures of a hybridoma cell line performed with in situ ammonia removal Schneider, M.;M.E. Alaoui;U. von Stockar;I.W. Marison https://doi.org/10.1016/S0141-0229(96)00122-6
  12. Appl. Biochem. Biotechnol. v.61 High-density hybridoma perfusion culture Banik, G.G.;C.A. Heath
  13. Biotechnol. Bioeng. v.32 Extended Monod kinetics for substrate, product, and cell inhibition Han, K.;O. Levenspiel https://doi.org/10.1002/bit.260320404