• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1212-1220
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• 2013

Because the cholesterol oxidase from Brevibacterium sp. M201008 was not as stable as the free enzyme form, it had been covalently immobilized onto chemically modified Sepharose particles via N-ethyl-N'-3-dimethylaminopropyl carbodiimide. The optimum immobilization conditions were determined, and the immobilized enzyme activity obtained was 12.01 U/g Sepharose-ethylenediamine. The immobilization of the enzyme was characterized by Fourier transform infrared spectroscopy. The immobilized enzyme exhibited the maximal activity at $35^{\circ}C$ and pH 7.5, which was unchanged compared with the free form. After being repeatedly used 20 times, the immobilized enzyme retained more than 40.43% of its original activity. The immobilized enzyme showed better operational stability, including wider thermal and pH ranges, and retained 62.87% activity after 20 days of storage at $4^{\circ}C$, which was longer than the free enzyme.

• 재활복지
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• 제22권4호
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• pp.153-172
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• 2018

본 연구의 목적은 특수교사를 대상으로 4차 산업혁명시대에 특수교육 분야가 무엇을 준비하고 어떻게 나아가야 하는지에 대한 방향을 찾기 위한 기초연구이다. 이러한 연구 목적을 위해 대구 경북에 재직 중인 특수교사 117명이 설문지 조사에 참여하였다. 주요 연구 결과를 살펴보면 첫째, 특수교사들은'4차 산업혁명'이 특수교육에 긍정적인 영향을 줄 것으로 인식하였으나 교육적 환경은 준비되어 있지 않은 것으로 인식하였다. 또한 특수교사들은 4차 산업혁명을 TV와 같은 매체를 통해 알게 되었고, 4차 산업혁명시대에 요구되는 특수교사의 역할과 역량이 더욱 커진다고 인식하였다. 또한 특수교사들은 4차 산업혁명 기반 특수교육이 유용할 것이며, 이를 위한 특수교육 프로그램 개발 및 보급이 요구된다고 인식하였다. 결론적으로 이 연구는 특수교사들이 바라보는 관점에서 4차 산업혁명을 이해하고 특수교육을 지원하기 위한 기초적 근거자료를 제공하는데 그 의미가 있다.

• 보건교육건강증진학회지
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• 제12권2호
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• pp.1-35
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• 1995

This study conducted from September 1 through October 30, 1993, in order to provide the data related to the industrial health policy and the development of health education program and the basic foundation. The data collected were analyzed in order to determine factors affecting knowledge and practice(KP) on health level of industrial workers. 3,396 workers were randomly selected from Kyungki, Inchun, Kwangju, Taejon, Taegu, Ulsan, Pohang, Masan and Changwon, Questionnaire forms were prepared and the personnel selected were interviewed by a graduate student with the aid of health management. As mentioned before, it was a serious problem that the industrial workers got the fairly low score of health KP and they did not practice what they know. Therefore it is necessary to establish the industrial policy and develop the education program for workers practice what they know and cooperate with related specialists, the management and the government authorities, Therefore we must ensure the good health of workers through a healthy home life, and a healthy work place and environment. Furthermore this will contribute to industrial peace and the development of korea.

• 한국안전학회지
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• 제22권4호
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• pp.72-82
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• 2007

The purpose of this study is to put forward revitalization of the Honorary Industrial Safety Inspector System by using the survey(2006.05) of the Honorary Industrial Safety Inspector in the Construction Industry. It can be summarized to develop the system as the following: 1) If there are neither labor union nor representative of workers in a company, workers must elect the representative of workers at first and then recommendation of the honorary industrial safety inspector by the representative of workers, 2) It should regulated in the industrial safety and health act about the tenure of the representative of workers and the process of election, 3) Prohibition the honorary industrial safety inspector as the employer members in the Industrial Safety and Health Committee and exclusion of both main office supervisor and subcontract-company supervisor from the appointment of the honorary industrial safety inspector, 4) Measures to realize the prohibitive regulation of unfavorable treatment on the grounds that the inspector has performed legitimate activities as an Honorary Industrial Safety Inspector, 5) Statutory regulation of the educations' hours(primary education & professional education), 6) Specification of the professional education and increase of the frequency, 7) Support of the government(the Ministry of Labor) to revitalize the honorary industrial safety inspector system, 8) Put in a statutory form the regular hours to carry out the work of the honorary industrial safety inspector in the workplace.

• 한국실천공학교육학회논문지
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• 제4권1호
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• pp.99-107
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• 2012

본 연구는 급변하는 산업요구변화에 탄력적으로 대응할 수 있는 대학교육 혁신방안으로 '기업연계형 장기현장 실습제도'를 제안하였다. 전문계 고등학교, 전문대학에서 그동안 실시하였던 현장실습의 문제점을 해결하고, 현장실습의 효과성을 제고하고자 체계적 현장훈련 모형에 기초하여 그 절차를 총괄계획수립-참여기업/학생 모집-참여기업과 학생 매칭-직무분석-프로그램 설계 및 개발-프로그램 운영-결과평가 및 피드백으로 세분화하였다. 또한 기업연계형 장기현장실습 프로그램을 운영방식을 2학기제(8학기)와 3학기제(11학기) 체제로 구분하여 제시하였다. 본 연구결과를 토대로 기업연계형 장기현장실습이 보다 효과적으로 운영되기 위해서 추가적으로 검토되어야 할 사항을 제시하였다.

• 한국실천공학교육학회논문지
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• 제4권2호
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• pp.104-109
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• 2012

협력 교육은 학업과 전공에 관련된 업무경험을 통합시킨 새로운 패러다임의 교육으로, 학생이 회사 조직에 잘 적응하고 현장실습을 통해 적절한 학습 경험을 얻기 위해서는 현장실습 과정 동안 학생들을 관리 감독하고 학생에게 업무나 업무 외적인 부분에 있어서 유익한 조언을 제공할 산업체 멘토가 필요하다. 산업체 멘토는 기본적으로 현장 실습생이 산업체에서 현장 학습을 효과적으로 수행할 수 있도록 도우는 역할을 수행하며 그 역할의 범위가 매우 넓다. 본 논문에서는 효율적인 산업체 멘토의 활용을 위해 산업체 멘토의 역할을 각각의 성격에 따라 구분하였고, 이에 따른 적절한 운영형태 및 현장 실습 단계에 따른 세부 업무 내용에 대해 기술하였다.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• Industrial Engineering and Management Systems
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• 제13권3호
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• pp.278-288
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• 2014

Master of Business Administration (MBA) education is growing rapidly in China, while many business schools in Japan are having difficulty in recruiting new students. The MBA education in China and Japan followed a different course and showed very different trends. In this article, we make a comparative study of MBA education in China and Japan. The emphasis is put on analyzing the reasons that caused the difference in the MBA education in the two Asian countries. We trace back the development of MBA education in China and Japan, examining the MBA education from the viewpoint of social environment, enterprise culture, and government support and control as well as the incentive and faculty management system of universities.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.534-538
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• 2013

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under $27{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity and $25^{\circ}C$ on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

• 과학교육연구지
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• 제45권1호
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• pp.90-104
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• 2021

본 연구는 4차 산업혁명 시대에 적합한 초등과학 교사 양성을 위해 필요한 초등 과학교육학 과목의 학습 내용에 대해 초등 교사들이 어떻게 생각하는지 알아보고자 온라인 설문을 실시하였다. 그 결과는 다음과 같다. 첫째, 현행 초등 과학교육학 과목의 학습 내용이 4차 산업혁명 시대에 적합하지 않으며 학습 내용 수정이 필요하다고 생각하는 초등교사가 다수 있었다. 초등 교사들이 그렇게 생각한 이유는 주로 과목의 학습 내용이 4차 산업혁명의 특징을 포함하지 못할 뿐만 아니라 시대변화를 반영하지 못하고 과거에 머물러있다고 생각했기 때문이었다. 둘째, 초등 교사들이 4차 산업혁명 시대에 적합한 초등 교사를 양성하는 데에 중요하다고 생각한 내용은 주로 학생의 흥미와 호기심, 과학 실험이나 탐구와 관련이 있는 것이었다. 그에 비해 삭제하거나 축소해야 한다고 생각하는 항목으로는 과학 학습이론, 과학과 교수·학습 모형, 과학의 본성, 영재아 지도가 있었다. 셋째, 초등 교사들이 초등 과학교육학 과목의 학습 내용으로 추가할 필요가 있다고 생각한 내용은 SSI 교육, 과학교육 관련 사회 변화와 미래예측, 첨단과학기술, STEAM 지도, 과학 분야 내의 통합교육이었다. 넷째, 4차 산업혁명 시대에 적합한 초등 교사 양성을 위해 초등 과학교육학 과목의 학습 내용으로 우선 도입해야 한다고 생각하는 항목은 SSI 교육, 과학 분야 내의 통합 교육, STEAM 지도와 과학과 핵심역량이었다. 그 외에 추가로 도입될 필요가 있는 내용으로는 소프트웨어교육, 안전교육, 프로젝트 학습법이 있었다.