• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1212-1220
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• 2013

Because the cholesterol oxidase from Brevibacterium sp. M201008 was not as stable as the free enzyme form, it had been covalently immobilized onto chemically modified Sepharose particles via N-ethyl-N'-3-dimethylaminopropyl carbodiimide. The optimum immobilization conditions were determined, and the immobilized enzyme activity obtained was 12.01 U/g Sepharose-ethylenediamine. The immobilization of the enzyme was characterized by Fourier transform infrared spectroscopy. The immobilized enzyme exhibited the maximal activity at $35^{\circ}C$ and pH 7.5, which was unchanged compared with the free form. After being repeatedly used 20 times, the immobilized enzyme retained more than 40.43% of its original activity. The immobilized enzyme showed better operational stability, including wider thermal and pH ranges, and retained 62.87% activity after 20 days of storage at $4^{\circ}C$, which was longer than the free enzyme.

• 재활복지
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• v.22 no.4
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• pp.153-172
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• 2018

This study is to examine the awareness of the 4th industrial revolution among special educational teachers as a preliminary study to explore the future special educational should continue forward in this new period. For the purposes of this study, a survey was conducted focusing on 117 special educational teachers in Korea. The major results were presented as follow. special educational teachers recognized that "the 4th industrial revolution" will have a positive effects on the special education, However, the level of understanding and interest in this was low. Moreover, Special teachers learned about 4th Industrial Revolution mainly through TV and media, recognized the needs for expert education on the fourth industrial revolution. Special teachers recognized that the Fourth Industrial Revolution was necessary for special education but was not realistically prepared. Special teachers said that programs for special education should be developed and distributed based on the 4th Industrial Revolution. In conclusion, The study is meaningful in understanding the Fourth Industrial Revolution from the perspective of special teachers and in providing basic evidence to support special education.

• Korean Journal of Health Education and Promotion
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• v.12 no.2
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• pp.1-35
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• 1995

This study conducted from September 1 through October 30, 1993, in order to provide the data related to the industrial health policy and the development of health education program and the basic foundation. The data collected were analyzed in order to determine factors affecting knowledge and practice(KP) on health level of industrial workers. 3,396 workers were randomly selected from Kyungki, Inchun, Kwangju, Taejon, Taegu, Ulsan, Pohang, Masan and Changwon, Questionnaire forms were prepared and the personnel selected were interviewed by a graduate student with the aid of health management. As mentioned before, it was a serious problem that the industrial workers got the fairly low score of health KP and they did not practice what they know. Therefore it is necessary to establish the industrial policy and develop the education program for workers practice what they know and cooperate with related specialists, the management and the government authorities, Therefore we must ensure the good health of workers through a healthy home life, and a healthy work place and environment. Furthermore this will contribute to industrial peace and the development of korea.

• Journal of the Korean Society of Safety
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• v.22 no.4
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• pp.72-82
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• 2007

The purpose of this study is to put forward revitalization of the Honorary Industrial Safety Inspector System by using the survey(2006.05) of the Honorary Industrial Safety Inspector in the Construction Industry. It can be summarized to develop the system as the following: 1) If there are neither labor union nor representative of workers in a company, workers must elect the representative of workers at first and then recommendation of the honorary industrial safety inspector by the representative of workers, 2) It should regulated in the industrial safety and health act about the tenure of the representative of workers and the process of election, 3) Prohibition the honorary industrial safety inspector as the employer members in the Industrial Safety and Health Committee and exclusion of both main office supervisor and subcontract-company supervisor from the appointment of the honorary industrial safety inspector, 4) Measures to realize the prohibitive regulation of unfavorable treatment on the grounds that the inspector has performed legitimate activities as an Honorary Industrial Safety Inspector, 5) Statutory regulation of the educations' hours(primary education & professional education), 6) Specification of the professional education and increase of the frequency, 7) Support of the government(the Ministry of Labor) to revitalize the honorary industrial safety inspector system, 8) Put in a statutory form the regular hours to carry out the work of the honorary industrial safety inspector in the workplace.

• The Journal of Korean Institute for Practical Engineering Education
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• v.4 no.1
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• pp.99-107
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• 2012

The purpose of this study was to develop a Industrial Professional Practice(IPP) at university education as a way of innovation strategy in order to effectively respond industrial needs in dynamic environment. A Industrial Professional Practice model was developed by using a structured on the job training model and had a 7 steps which were "planning Industrial Professional Practice, selecting enterprises and students, matching enterprises and students, job analysis, design and development program, implementation, evaluation and feedback. Based on the Industrial Professional Practice model, the directions for the future studies was discussed.

• The Journal of Korean Institute for Practical Engineering Education
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• v.4 no.2
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• pp.104-109
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• 2012

Cooperative education, a new paradigm of education, is a educational method of combining classroom-based education with practical work experience. To obtain appropriate learning experience during the work period, industrial mentors who provide beneficial advice and instruct students, are needed. Industrial mentors, basically, performs various roles in helping students to make the experiential learning efficiently, and the scope of their role is broad. In this paper, we classified the role of industrial mentors, proposed appropriate operating models and described the detail of their responsibilities.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• Industrial Engineering and Management Systems
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• v.13 no.3
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• pp.278-288
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• 2014

Master of Business Administration (MBA) education is growing rapidly in China, while many business schools in Japan are having difficulty in recruiting new students. The MBA education in China and Japan followed a different course and showed very different trends. In this article, we make a comparative study of MBA education in China and Japan. The emphasis is put on analyzing the reasons that caused the difference in the MBA education in the two Asian countries. We trace back the development of MBA education in China and Japan, examining the MBA education from the viewpoint of social environment, enterprise culture, and government support and control as well as the incentive and faculty management system of universities.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.534-538
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• 2013

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under $27{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity and $25^{\circ}C$ on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

• Journal of Science Education
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• v.45 no.1
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• pp.90-104
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• 2021

This study conducted an online survey to understand what elementary school teachers think about the learning contents of elementary science education subjects needed to train elementary science teachers suitable for the era of the 4th Industrial Revolution. The results are as follows: First, there were many elementary school teachers who thought that the current learning content of elementary science education was not suitable for the era of the 4th Industrial Revolution and that it needed to modify the learning content. Many of the teachers said that the learning content of the subject did not include the characteristics of the 4th Industrial Revolution, but also did not reflect the changes of the times and remained in the past. Second, the content that elementary school teachers thought was important in training elementary school teachers suitable for the era of the 4th Industrial Revolution was mainly related to the interests and curiosity of students, and scientific experiments or inquiry. On the contrary, the items that they thought should be deleted or reduced included science learning theory, science teaching/learning model, nature of science, and guidance for gifted children. Third, the contents that elementary school teachers thought needed to be added as learning content of elementary science education subjects were SSI education, science education-related social change and future prediction, advanced science technology, STEAM guidance, and integrated education within the science field. Fourth, in order to train elementary school teachers suitable for the era of the 4th Industrial Revolution, the contents that they thought should be introduced first as learning content of elementary science education subjects were SSI education, integrated education within the science field, STEAM guidance, and core competencies. Other contents that need to be introduced were software education, safety education, and project learning methods.