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단삼 유래 Tanshinone IIA가 3T3-L1 세포의 아포토시스 유도와 지방형성 억제에 미치는 영향 (Effects of Tanshinone IIA from Salvia Miltiorrhiza Bunge on Induction of Apoptosis and Inhibition of Adipogenesis in 3T3-L1 Cells)

  • 정승일;이종우;장선일
    • 동의생리병리학회지
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    • 제23권6호
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    • pp.1409-1415
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    • 2009
  • Obesity is especially a serious health problem in industrialized countries, because it is considered to be a risk factor associated with the genesis or development of various metabolic diseases, including cardiovascular disease and type 2 diabetes mellitus. The purpose of this study was to investigate the effects of tanshinone IIA from Salvia miltiorrhiza Bunge on induction of apoptossis and inhibition of adipogenesis in in 3T3-L1 preadipocytes and adipocytes. The results demonstrated that tanshinone IIA decreased cell population growth of 3T3-L1 preadipocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of 3T3-L1 preadipocytes exposed to tanshinone IIA showed that apoptotic cells increased in a timeand dose-dependent manner. Treatment with tanshinone IIA decreased the number of normal cells and increased the number of apoptotic cells in a dose-dependent manner. The induction of apoptosis in 3T3-L1 preadipocytes by tanshinone IIA was mediated through the activation of caspase-3 and Bax, and then through the cleavage of PARP and the down-regulation of Bcl-2. Moreover, tanshinone IIA significantly decreased the amount of intracellular triglycerides and GPDH (glycerol-3-phosphate dehydrogenase) activity in 3T3-L1 adipocytes. Our results suggest that tanshinone IIA efficiently induces apoptosis and inhibits adipogenesis in 3T3-L1 preadipocytes and adipocytes.

지리산지역 고로쇠나무 및 거제수(거자수) 나무의 수액성분에 관하여 -Mineral과 Sugar성분에 관하여- (A Study on the Ingredients in the Sap of Acer mono MAX. and Betula costata T. in Mt. Jiri Area -On the Components of Mineral and Sugar-)

  • 서화중;김충모;정두례
    • 한국식품영양과학회지
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    • 제20권5호
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    • pp.479-482
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    • 1991
  • 고로쇠 수액의 주된 양이온 함량분포는 $Ca^{++},\;K^{+},\;Mg^{++},\;Na^{+}$이 각각 3.19, 1.74, 0.37, 0.24mEq/l이고 그 중 Ca와 K가 major ion이다. 주된 음이온은 ${SO_4}^{--}$가 2.0mEq/l, $Cl^{-}$이 0.19mEq/l 검출 되었다. 거제수 수액은 $K^{+},\;Ca^{++},\;Mg^{++),\;Na^{+}$이 각각 3.89, 1.95, 0.47, 0.42mEq/l로서 $K^{+}$의 비율이 월등히 높고 다음은 $Ca^{++}$ 순위이다. 음이온은 ${SO_4}^{--}$가 1.84mEq/l, $Cl^{-}$이 0.45mEq/l로서 ${SO_4}^{--}$가 높다. 이 양수액의 $K^{+},\;Ca^{++}와\;{SO_4}^{--},\;Cl^{-}$농도 상호간의 비율을 비교시 거제수가 고로쇠보다 $K^{+}$의 비율이 높고 $Ca^{++}$량은 상대적으로 적은 반면 ${SO_4}^{--}$는 고로쇠와 거의 비슷한 값이다. Sugar함량에서 고로쇠가 sucrose 2.7%함유로 비교적 감미가 높고 거제수는 포도당과 과당이 각각 0.097%, 0.305%함유되어 있다. 미량원소는 Cu, Zn, Mn에서 고로쇠와 거제수가 각각 0.057~3.038mg/l, 0.483~1.584mg/l, 5.507~4.354mg/l이고 거제수에서 Fe가 2.511mg/l 검출되었다.

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Production of Rosmarinic Acid, Lithospermic Acid B, and Tanshinones by Suspension Cultures of Ti-Transformed Salvia miltiorrhiza Cells in Bioreactors

  • Zhong, Jian-Jiang;Hui Chen;Feng Chen
    • Journal of Plant Biotechnology
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    • 제3권2호
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    • pp.107-112
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    • 2001
  • The kinetics of Ti-transformed Salvia miltiorrhiza cell cultures was studied in 250-$m\ell$ shake flasks by using B5 medium with addition of 30 gfL of sucrose. In the cell cultures, the maximum cell mass obtained was 11.5 g DW/L on day 15. The highest amount of phenolic compounds - rosmarinic acid (RA) and lithospermic acid B (LAB) reached 871.3 mg/L (day 15) and 121.3 mg/L (day 13), respectively. The total tanshinone production, i.e., intracellular plus extracellular cryptotanshinone, tanshinone 1, and tanshinone IIA, was 5.3 mg/L on day 13. For the cultivations in 2.4-L stirred bioreactors, the residual sugar level and medium conductivity were a little higher in a small turbine impeller reactor ($T_s$) than those in a large turbine impeller reactor ($T_L$), while a higher cell density was obtained in the $T_L$. For the production of tanshinones and phenolics, better results were obtained in the $T_L$ than in the $T_s$. In the $T_L$, similar or even a little higher production titers of tanshinones and phenolic compounds were achieved compared to those in the flasks. The results suggest that the shake flask results could be successfully scaled up to the $T_L$ reactor. Such a large impeller reactor like $T_L$ may be better than a small impeller one for the large-scale production of the valuable metabolites by the suspension cultures of Ti transformed S.miltiorrhiza cells. This is considered due to the beneficial culture environment in the $T_L$, such as low shear rates as estimated theoretically.

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LIE IDEALS IN TRIDIAGONAL ALGEBRA ALG𝓛

  • Kang, Joo Ho
    • 대한수학회보
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    • 제52권2호
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    • pp.351-361
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    • 2015
  • We give examples of Lie ideals in a tridiagonal algebra $Alg\mathcal{L}_{\infty}$ and study some properties of Lie ideals in $Alg\mathcal{L}_{\infty}$. We also investigate relationships between Lie ideals in $Alg\mathcal{L}_{\infty}$. Let k be a fixed natural number. Let $\mathcal{A}$ be a linear manifold in $Alg\mathcal{L}_{\infty}$ such that $T_{(2k-1,2k)}=0$ for all $T{\in}\mathcal{A}$. Then $\mathcal{A}$ is a Lie ideal if and only if $T_{(2k-1,2k-1)}=T_{(2k,2k)}$ for all $T{\in}\mathcal{A}$.

$\delta$$^{15}$ N을 이용한 제주도 지하수 중의 질산염 오염원 조사 (Estimation of Nitrate Sources in Cheju Island Groundwater using $\delta$$^{15}$ N)

  • 송영철;고용구;유장걸
    • 대한지하수환경학회지
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    • 제6권3호
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    • pp.107-110
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    • 1999
  • 제주도 지하수중 질산염농도가 높은 지점을 선정하여 1995년부터 1996년까지 4차에 걸쳐 질소안정등위원소의 자연존재를 측정하고. 오염원별 기여율을 계산한 결과는 다음과 같다. 화학비료에 의한 영향이 뚜렸하게 나타나는 지점은 T-3, L-1. O-1∼O-4. F-2∼F-5. G-2의 11개소로써 화학비료 질소성분이 지하수 질산염농도 중 약 60% 이상을 차지하는 것으로 조사되었다. 그리고. 동물성 유기물질에 의한 영향이 많은 지하수는 T-4, T-5. G-2지점 3개소이며, T-4, T-5지점은 생활하수에 의한 영향으로, G-2지점은 쓰레기매립장 침출수의 영향으로 사료된다. 또한. 화학비료와 동물성 유기물질에 의한 영향을 비슷하게 받는 지하수는 T-1, T-2. L-2. F-1지점 4개소로 조사되었다.

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활엽수림, 침엽수림 및 혼효림 지역의 강우시 비점오염물질 유출특성 (Runoff Characteristics of Non-point Source Pollutants from Different Forest Types During Rainfall Events)

  • 신민환;신동석;이재운;최재완;원철희;서지연;최용훈;최중대
    • 한국물환경학회지
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    • 제26권3호
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    • pp.507-517
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    • 2010
  • Long-term monitoring was conducted to identify the runoff characteristics of non-point source according to the three forest types (deciduous forest, coniferous forest and mixed forest) in this study. Rainfall events of each deciduous forest, coniferous forest, and mixed forest were 10, 8, 12, respectively. Average runoff depth and coefficients of each forest type were founded to be coniferous forest and were followed by others in turns : deciduous forest, and mixed forest because various conditions (i.e., rainfall property, Antecedent Precipitation Index (API), soil property, slope, and forest management) could change runoff characteristics. In the analysis of the first flush phenomenon, it showed that SS and T-P were sensitive for the first flush phenomenon. The first flush phenomenon of them were showed differently by rainfall intensity, rainfall duration, and amount of rainfall. The research results indicated that range of the Event Mean Concentration (EMC) values in deciduous forest were 0.8~2.4 mg/L for $BOD_5$, 2.0~13.4 mg/L for $COD_{Mn}$, 1.3~2.9 mg/L for DOC, 1.150~3.913 mg/L for T-N, 0.010~0.350 mg/L for T-P and 3.1~291.8 mg/L for SS and in coniferous forest were 0.8~2.2 mg/L for $BOD_5$, 1.9~3.6 mg/L for $COD_{Mn}$, 1.0~2.0 mg/L for DOC, 1.025~2.957 mg/L for T-N, 0.002~0.084 mg/L for T-P and 0.8~5.4 mg/L for SS. Also, range of the EMC values in mixed forest were 1.3~2.3 mg/L for $BOD_5$, 2.4~4.8 mg/L for $COD_{Mn}$, 1.1~2.1 mg/L for DOC, 0.385~2.703 mg/L for T-N, 0.016~0.080 mg/L for T-P and 2.3~30.0 mg/L for SS.

THE ASYMPTOTIC BEHAVIOUR OF THE AVERAGING VALUE OF SOME DIRICHLET SERIES USING POISSON DISTRIBUTION

  • Jo, Sihun
    • East Asian mathematical journal
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    • 제35권1호
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    • pp.67-75
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    • 2019
  • We investigate the averaging value of a random sampling of a Dirichlet series with some condition using Poisson distribution. Our result is the following: Let $L(s)={\sum}^{\infty}_{n=1}{\frac{a_n}{n^s}}$ be a Dirichlet series that converges absolutely for Re(s) > 1. If $X_t$ is an increasing random sampling with Poisson distribution and there exists a number $0<{\alpha}<{\frac{1}{2}}$ such that ${\sum}_{n{\leq}u}a_n{\ll}u^{\alpha}$, then we have $${\mathbb{E}}L(1/2+iX_t)=O(t^{\alpha}{\sqrt{{\log}t}})$$, for all sufficiently large t in ${\mathbb{R}}$. As a result, we get the behaviour of $L({\frac{1}{2}}+it)$ such that L is a Dirichlet L-function or a modular L-function, when t is sampled by the Poisson distribution.

골프장 페어웨이에 적합한 크리핑 벤트그래스 품종 선발 (Selection of Creeping Bentgrass(Agrostis Palustris Huds.) Cultivar for Fairway in Golf Course)

  • 차영기;김경덕;박대섭;김두환
    • 아시안잔디학회지
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    • 제25권2호
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    • pp.147-152
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    • 2011
  • 본 연구는 페어웨이 조성에 적합한 크리핑 벤트그래스 품종을 선발하기 위해 수행 되었다. 'Shark'와 'CY-2' 품종의 시각적 품질이 가장 우수하였으며, 크리핑 벤트그래스 페어웨이에서 가장 많이 고려 되어야 하는 하절기 시각적 품질은 'Shark', 'CY-2', 'T-1', 'Alpha', 'L-93' 순이었다. 포지 조성 초기 엽록소함량이 가장 우수한 품종은 'Shark'와 'CY-2' 그리고 'T-1' 이다. 'T-1'은 하절기 색상이 매우 짙게 유지가 되었다. 조성 초기부터 'CY-2'와 'Shark'의 뿌리 길이가 길고 우수했으며 잔뿌리가 많아 수분 흡수가 매우 용이할 것으로 판단된다. 조성 초기부터 가장 우수한 식생 지수를 보인 것은 'Shark'와 'L-93' 그리고 'CY-2'였다. 가장 높은 밀도를 보인 품종은 'T-1' 이였으며 다른 품종과 다르게 하절기 밀도가 떨어지지 않고 오히려 증가하였다. 'T-1' 다음으로 'Shark'가 높은 밀도를 보였고, 'CY-2', 'Alpha', 'L-93'의 순으로 밀도가 높았다. 실험기간 동안 탄저병, 브라운패취, 달라스팟이 발병 하였으며 'CY-2'가 가장 내병성이 강하였고 3가지 병 모두 내병성을 보였다. 본 연구 결과 5개 품종 중 'CY-2'와 'Shark'가 골프장 페어웨이용으로 가장 적합하였다.

GENERALIZED BROWNIAN MOTIONS WITH APPLICATION TO FINANCE

  • Chung, Dong-Myung;Lee, Jeong-Hyun
    • 대한수학회지
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    • 제43권2호
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    • pp.357-371
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    • 2006
  • Let $X\;=\;(X_t,\;t{\in}[0, T])$ be a generalized Brownian motion(gBm) determined by mean function a(t) and variance function b(t). Let $L^2({\mu})$ denote the Hilbert space of square integrable functionals of $X\;=\;(X_t - a(t),\; t {in} [0, T])$. In this paper we consider a class of nonlinear functionals of X of the form F(. + a) with $F{in}L^2({\mu})$ and discuss their analysis. Firstly, it is shown that such functionals do not enjoy, in general, the square integrability and Malliavin differentiability. Secondly, we establish regularity conditions on F for which F(.+ a) is in $L^2({\mu})$ and has its Malliavin derivative. Finally we apply these results to compute the price and the hedging portfolio of a contingent claim in our financial market model based on a gBm X.