• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.862-868
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• 2014

Certain Escherichia coli (E. coli) strains have the ability to cause diarrheal disease. Five types of diarrheagenic E. coli have been identified, including EHEC, ETEC, EPEC, EAEC, and EIEC. To detect these five diarrheagenic types rapidly, we developed a one-step multiplex PCR (MP-PCR) assay using nine primer pairs to amplify nine virulence genes specific to the different virotypes, with each group being represented (i.e., stx1 and stx2 for EHEC, lt, sth, and stp for ETEC, eaeA and bfpA for EPEC, aggR for EAEC, and ipaH for EIEC). The PCR primers were constructed using MultAlin. The sensitivity and specificity of the constructed multiplex PCR primers were measured using DNA isolated from diarrheagenic E. coli strains representing each group. The limits of detection were as follows: $5{\times}10^1CFU/ml$ for EHEC, $5{\times}10^3CFU/ml$ for ETEC expressing lt and sth, $5{\times}10^4CFU/ml$ for ETEC expressing stp, $5{\times}10^2CFU/ml$ for EPEC, $5{\times}10^4CFU/ml$ for EAEC, and $5{\times}10^2CFU/ml$ for EIEC. To confirm the specificity, C. jejuni, C. perfringens, S. Typhimurium, V. parahaemolyticus, L. monocytogenes, Y. enterocolitica, B. cereus, and S. aureus were used as negative controls, and no amplification was obtained for these. Moreover, this kit was validated using 100 fecal samples from patients with diarrhea and 150 diarrheagenic E. coli strains isolated in Korea. In conclusion, the multiplex PCR assay developed in this study is very useful for the rapid and specific detection of five diarrheagenic E. coli types. This single-step assay will be useful as a rapid and economical method, as it reduces the cost and time required for the identification of diarrheagenic E. coli.

• 한국콘텐츠학회논문지
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• 제14권6호
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• pp.307-318
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• 2014

이 연구에서는 일반인이 건강증진 메시지 디자인과 관련하여 핵심정보 제시, 내용의 유용성, 형식, 직관성의 중요성을 어떻게 인식하는지와 현재 식품의약품안전처에서 발간하는 웹진의 텍스트와 시각자료가 위 네 가지 측면에서 얼마나 효과적으로 디자인되었다고 인식하는지를 조사하였다. 그리고 이러한 인식에 영향을 미치는 개인특성 변인이 무엇인지를 탐색적으로 연구하였다. 이를 위해 294명의 성인들을 대상으로 설문조사를 실시하였으며, 중요도 및 실행도 분석(Importance-Performance Analysis)을 실시하였다. 그 결과 연구참여자들은 텍스트 디자인에서 내용의 유용성을, 시각자료 디자인에서 핵심정보 제시를 가장 중요한 항목으로 인식하였으며, 텍스트와 시각자료 모두 형식 측면에서의 실행도가 가장 높게 나타났다. 시각자료의 핵심정보 제시와 직관성은 가장 중점적으로 개선해야 할 원리인 것으로 나타났다. 그리고 개인의 건강관심도가 건강증진 메시지 디자인 원리의 중요도와 실행도 인식에 가장 큰 영향을 미치는 것으로 나타났다. 이러한 연구결과에 기반하여 효과적인 건강증진 메시지 개발을 위한 원리와 전략에 대해 논의하였다.

• 대한영양사협회학술지
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• 제7권4호
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• pp.410-425
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• 2001

This study was designed to assess the waste management practices, source-reduction programs and related tasks in elementary school foodservice in Seoul. and Kyonggi Province Areas. A questionnaire mailed to 910 school foodservice managers in Seoul and Kyonggi province: a 202% (N=184) response rate was obtained. About 83.7% of school foodservice managers were responsible for managing solid waste. Most foodservice operations take the responsibility of food waste but not packaging wastes. The average disposal cost per month per person was about 19 won and income was about 35 won. Leftovers were mainly disposed with platewaste. Platewaste was reused for the livestook feed(68.0%), and some platewaste was composted(11.6%). Source reduction programs implemented in half of school foodservice operations were adjustment of portion size', checking the plate waste', 'working with classroom teachers on waste reduction campaign', 'providing information leaflet on food waste minimization', posting education materials, 'classroom instruction about food waste minimization', and 'improving cooking method. According to factor analysis based on importance rating. slid waste management activities of elementary school foodservice operations were divided to 7 dimensions such as communication and cooperation with others', 'employee training', 'product quality control, 'yield control', plate waste control', 'purchasing', and 'public relations'. As a result of Important-Performance Analysis(IPA), sine dimensions such as 'communication and cooperation with others' and 'plate waste control were identified in Focus Here' quardrant area which need more enhanced performance.

• 한국조리학회지
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• 제19권4호
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• pp.77-93
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• 2013

본 연구는 조리 관련 학과를 전공하고 있는 일부 4년제 대학생을 대상으로 조리 실습 교육에 대한 인식 수준을 조사하여 조리교육의 발전 방안을 모색하고자 하는 목적으로 수행되었다. 교육은 지식을 전달하고 학습자의 특정한 태도와 행동을 형성하기 위하여 의도적이면서 조직적으로 학습을 유도하여 지식, 정보, 적절한 상황이나 조건을 준비하는 것으로서 조리교육 만족도와 요구도를 살펴 조리 실습교육의 의미와 학생의 입장에서 조리학습교육을 이끌어나가기 위하여 조사 분석하였다. 이에 본 연구에서는 특정한 상황에 맞는 대학교육서비스 품질 측정도구를 개발하고, 이를 통하여 대학 조리 실습교육 품질을 결정하는 요인을 도출해 보았으며, 26개의 품질속성에 대해 중요도-만족도 변환지수를 이용하여 해석한 결과, 저학년일 경우 실습흥미, 진로필요, 현장실무 레시피, 전공 관심 증가, 전공학과만족 순으로 중요하다고 인식하였으며 고학년일 경우 현장 레시피, 실습흥미 재료시범 순으로 중요하다고 하였다.

• 청정기술
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• 제22권1호
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• pp.9-15
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• 2016

Al/(Al+Zr)의 몰 비를 달리하여 xAl-yZr 산화물 촉매를 공침법으로 제조하였고, 모델반응으로 이소프로판올 탈수반응을 수행하였다. 제조된 촉매는 X-선 회절분석, 시차 열분석법, 질소 흡착법, 암모니아 승온탈착법, 이소프로판올 승온탈착법 등의 특성분석을 수행하였다. 지르코니아에 알루미늄 종을 첨가하면 상대적으로 넓은 비표면적을 갖는 작은 입자를 얻을 수 있으며 지르코니아의 결정상변화를 억제시켰다. 암모니아 승온탈착으로 알루미늄의 몰 비가 증가함에 따라 상대적인 산양이 증가함을 확인하였고, 이소프로판올탈수반응에서 촉매 활성 또한 증가하였다. 이러한 촉매활성은 촉매의 비표면적, 산점, 상대적으로 용이한 이소프로판올의 탈착과 연관시킬 수 있었다.

• 한국항만경제학회지
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• 제33권1호
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• pp.61-74
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• 2017

본 연구는 부산항 국제 선용품 공급업의 경쟁력을 도모하기 위한 일환으로서 정보화 역량을 분석하는 것을 목적으로 한다. 구체적으로는 최근 추진되고 있는 선용품 공급업체의 종합정보관리시스템의 성공적인 도입과 정착을 위한 선결과제로서 정보화 역량을 분석하고자 한다. 이러한 과정을 통해 종합정보관리시스템을 수용하고 활용하는데 필요한 당사자들의 정보화 역량을 실증적으로 파악하여 종합정보시스템 구축과 더불어 추진되어야 할 몇 가지 과제를 제안하고자 한다. 연구의 목적을 달성하기 위해, 국가직무능력표준을 바탕으로 평가기준을 수립하였으며, 중요도-수행도 분석(Importance-Performance Analysis)기법을 활용하였다. 실제 기업을 대상으로 조사 자료를 수집하여 분석한 결과, 정보화 역량이 전반적으로 취약한 것으로 나타났다. 특히, 정보처리능력이 다른 영역에 비해 매우 취약하다는 점을 발견할 수 있었다. 따라서, 종합정보관리시스템의 성공적인 도입을 위해서는 정보화 재교육 프로그램과 전담 지원조직을 구성하는 것이 필요할 것으로 사료된다.

• 농촌계획
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• 제22권1호
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• pp.101-111
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• 2016

The purpose of this study is to investigate the roadside agricultural product shops located in Miryang city, and to find out the current situation on how people rate the importance and satisfaction levels of various aspects through surveys on sellers and consumers. With this information, the main problems and possible solutions are analyzed with the Important Performance Analysis(IPA) method. First, building parking spaces for customers should be prioritized to improve safety. Because many roadside shops for agricultural products are located alongside two-lane roads, sufficient parking spaces are needed for the consumers' safety. Second, to enhance the rural landscape, consumers express that improving store quality and integrating more with the environment are important. Store owners should take more care in maintaining store quality so that customers do not experience inconvenience while shopping. Futhermore, institutional guidelines and standards must be implemented to allow for a more harmonious relationship between these shops and the environment. Third, both sellers and consumers think that taste, price, and freshness are important aspects of roadside agricultural product shops. However, while sellers and consumers both agree that the price is the most important factor, there is a discrepancy between these two groups regarding the satisfactory price level. The sellers do not agree that their products are being sold at the fair price. This can be interpreted as sellers believe that the prices are set too low. Because pricing is important aspect and also a very delicate issue, mid to long term consideration and study will be needed to improve roadside shops for agricultural products in the future.

• Molecular & Cellular Toxicology
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• 제4권4호
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• pp.323-330
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• 2008

Surrogate tissue analysis incorporating -omics technologies has emerged as a potential alternative method for evaluating toxic effect of the tissues which are not accessible for sampling. Among the recent applications, blood including whole blood, peripheral blood lymphocytes and peripheral blood mononuclear cells (PBMCs) was suggested as a suitable surrogate tissue in determining toxicant exposure and effect at the pre- or early clinical stage. In this application, we investigated transcriptomic profiles in astemizole treated Cynomolgus monkey's PBMCs. PBMCs were isolated from 4-6 years old male monkeys at 24 hr after administration45 Helvetica Light (10 mg/kg, 30 mg/kg). Gene expression profiles of astemizole treated monkey's PBMCs were determined using Affymetrix $GeneChip^{(R)}$ Human Genome U133 plus 2.0 arrays. The expression levels of 724 probe sets were significantly altered in PBMCs at 10 or 30 mg/kg after astemizole administration following determination of paired t-test using statistical criteria of ${\geq}$$1.5-fold changes at P<0.05. Gene expression patterns in PBMCs showed a considerable difference between astemizole 10 and 30 mg/kg administration groups in spite of an administration of the same chemical. However, close examination using Ingenuity Pathway Analysis (IPA) software revealed that several gene sets related to cardiotoxicity were deregulated at astemizole 10 and 30 mg/kg administration groups. The deregulation of cardiac hypertrophy related genes such as TXN, GNAQ, and MAP3K5 was observed at 10 mg/kg group. In astemizole 30 mg/kg group, genes involved in cardiotoxicity including cardiac necrosis/cell death, dilation, fibrosis, and hypertrophy were also identified. These results suggest that toxicogenomic approach using PBMCs as surrogate tissues will contribute to assess toxicant exposures and identify biomarkers at the pre-clinical stage.

• 공학교육연구
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• 제17권1호
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• pp.57-64
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• 2014

This study has a purpose mainly to analyze the team interaction change by the duration of time in the Capstone-Design activities according to MBTI Modes. Study objects are four students of Mechanical Engineering at School of Engineering in C University located in Daejeon, and the team interaction change was analyzed through IPA (Interaction Process Analysis) method. From the result, first, ESTP showed the change of increase in interaction by the time duration of initial, mid, late periods in 'social-emotional area: positive' and 'task area: question', and the change of decrease by the same time duration of periods in 'task area: solution'. Also, there was no change in 'social-emotional area: negative' because there was no interaction. Second, ESFJ showed the change of decrease in interaction by the time duration of initial, mid, late periods in 'social-emotional area: positive' and 'task area: question', and the change of increase by the same time duration of periods in 'task area: solution' and 'social-emotional area: negative'. Third, ISTJ showed the change of decrease in interaction by the time duration of initial, mid, late periods in 'social-emotional area: positive', 'task area: question' and 'social-emotional area: negative', and the change of increase by the same time duration of periods in 'task area: solution'. Fourth, ENFP showed the change of decrease by the time duration of initial, mid, late periods in 'social-emotional area: positive', 'task area: solution' and 'social-emotional area: negative', and the change of increase by the same time duration of periods in 'task area: question'.

• Journal of Construction Engineering and Project Management
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• 제1권3호
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• pp.9-17
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• 2011

Web-based PMIS (Project Management Information System) has played significant role in construction management processes in Korea. As the use of web-based PMIS increases, regular quality assessment to identify user's requirements is necessary. However, there have been rare research efforts for quality assessment of web-based PMIS. This study aims to assess the quality of web-based PMIS, especially ASP-PMIS (Application Service Provider based PMIS) that is widely used in Korean construction industry. The assessment factors of ASP-PMIS quality were adopted from previous research and empirically confirmed using exploratory factor analysis. ISA (Importance-Satisfaction Analysis), which is a variation of original IPA (Importance-Performance Analysis), were selected for the assessment and analysis method in this research. A total of 253 completed questionnaires, composed of 23 assessment items, were collected from the ASP-PMIS users in Korea (construction managers and constructors), and they were used to analyse the quality of the systems. Some possible contributions of this research are: it introduces a simple and easy-to-use tool for assessing the quality of ASP-PMIS with a set of quality assessment factors that are selected from previous researches and empirically tested; it provides the quality assessment results of ASP-PMIS in Korea so that ASP-PMIS providers in Korea can understand the users' opinion on their systems; it also identified that the urgent factors that require immediate attention to improvement. However, further researches are to be required on the following areas: enhancing the quality assessment factors in terms of their relation to the success of ASP-PMIS and to the users' performance; assessing and analyzing the quality of individual ASP-PMIS; establishing continuous improvement systems institutionally and instrumentally.