• BMB Reports
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• 제38권6호
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• pp.763-765
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• 2005

A new simplified procedure for identifying human plasmin was developed using a DTT copolymerized agarose stacking gel (ASG) system. Agarose (1%) was used for the stacking gel because DTT inhibits the polymerization of acrylamide. Human plasmin showed the lowest activity at pH 9.0. There was a similar catalytically active pattern observed under acidic conditions (pH 3.0) to that observed under alkaline conditions (pH 10.0 or 11.0). Using the ASG system, the primary structure of the heavy chain could be established at pH 3.0. This protein was found to consist of three fragments, 45 kDa, 23 kDa, and 13 kDa. These results showed that the heavy chain has a similar structure to the autolysed plasmin (Wu et al., 1987b) but there is a different start amino acid sequence of the N-termini.

• 대한의생명과학회지
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• 제4권2호
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• pp.129-135
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• 1998

Cytochrome P45O 2El (CYP2El)의 retropseudogene이 사람에 존재하는지 확인하기 위해 혈액에서 분리한 DNA를 주형으로 한 polymerase chain reaction (PCR)을 수행하였다. Primer는 CYP2E1 유전자를 기초하여 여러개 제작하되 적절한 조합에 따라 정상유전자와 retropseudogene이 동시에 증폭될 수 있도록 고안하였다. 본 실험의 결과 그동안 예상은 되었지만 DNA차원에서 미처확인되지 못하였던 CYP2E1의 retropseudogene을 직접 증폭해낼 수 있었다. 세부 구조는 Southern blotting과 DNA 염기서열 결정에서 최종 분석되었다. PCR 반응으로 증폭된 부분에서는 염기서열이 mRNA와 완전히 일치하고 있는 점으로 미루어서 CYP2E1의 retropseudogene은 비교적 최근에 발생했을 것으로 추정되었다.

• 복식
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• 제63권7호
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• pp.134-147
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• 2013

Today, the convergence of fashion design and digital technology has become a popular method and accordingly been tried variously in the fashion area. This study aimed to analyze the character of the collaboration with fashion and technology, and the meaning of technology as emotional expression methods. Selected designer's collections, literature and other related studies were reviewed in order to analyze the generative structure of the meaning and the binary-opposition in the convergence of body, fashion design and technology. Literatures and selected designer's collections were reviewed and quantitatively studies were performed to classify the convergence of human bodies, fashion design and technology through Greimas Semiotics rectangle based on binary-opposition of meaning and isotophy analysis. The research presents three types of fashion technology methods: mechanical movement, light/digital media, and virtual image. The convergence of fashion and technology was classified as the direct convergence and the indirect convergence. The direct convergence was characterized by variability and has automatic, independent movement, but the indirect convergence was shown closed and to have contradistinctive images.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2317-2325
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• 2014

P-glycoprotein (P-gp) is a member of the ATP-Binding Cassette transporter superfamily and mediates transmembrane efflux of many drugs. Since it is involved in multi-drug resistance activity in various cancer cells, the development of P-gp inhibitor is one of the major concerns in anticancer therapy. Human P-gp protein has at least two "functional" drug binding sites that are called "H" site and "R" site, hence it has multi-binding-specificities. Though the amino acid residues that constitute in drug binding pockets have been proposed by previous experimental evidences, the shapes and the binding poses are not revealed clearly yet. In this study, human P-gp structure was built by homology modeling with available crystal structure of mouse P-gp as a template and docking simulations were performed with inhibitors such as verapamil, hoechst33342, and rhodamine123 to construct the interaction between human P-gp and its inhibitors. The docking simulations were performed 500 times for each inhibitor, and then the interaction frequency of the amino acids at the binding poses was analyzed. With the analysis results, we proposed highly contributing residues that constitute binding pockets of the human P-gp for the inhibitors. Using the highly contributing residues, we proposed the locations and the shapes of verapamil binding site and "R" site, and suggested the possible position of "H" site.

• 디지털산업정보학회논문지
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• 제7권2호
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• pp.147-159
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• 2011

In this paper the demand and supply of labor are forecasted over the next 10 years for customized nurturing human resource focused on Manufacturing Industry in Chung-Nam Province. Despite that the industrial structure is rapidly changing, industrial labors are nurturing on the basis of past industrial structure. This research is conducted for reducing mismatched labors throughout forecasting human resources until 2020. As a practical approach, the BLS Methodology is partially utilized. And the previous researches and official statistics data are reviewed. In conclusion, this study presents that more human resources on Manufacturing Industry than other Industries will be needed in Chung-Nam province. In details, it shows that there will be required more Industrial labors for strategic industries for examples, Audio and Video related industry, and Car related industry which is propelling by overall local government. In additions, policy implications are developed by analyzing current status and forecasting the labor demand and supply in the Chung-Nam Manufacturing sector.

• 대한간호학회지
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• 제31권4호
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• pp.525-537
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• 2001

The Purpose of the study was to understand the experience of chronic renal failure patients for the qualified individual care for them. The purpose of this study was to explore the experience of patients living with chronic renal failure and to identify the meaning and structure of their experience. The subjects were four patients, two females and two males. The age range was from 21 to 54. Data was collected with a few in-depth interviews by the authors until the data was fully saturated. The framework and methodology of this study was based on Parse′s "Human Becoming methodology," an existential phenomenological research methodology. The findings of this study were as follows. Three experience structures of chronic renal failure patients were : 1. Sufferings and conflicts originated in the frustration caused by uncurable disease. 2. Dependence upon God and significant others with complex emotions. 3. Acceptance of sufferings, emerging hope for serving people, and gratitude for living. In conclusion the experience of chronic renal failure patients could be described from the findings (three structures) as "Experiencing the sufferings, conflicts originated in the frustration caused by uncurable disease, dependence upon God and significant others with complex emotion, acceptance of the suffering and hope for serving people, and gratitude for living." The three structures of the lived experience of patients with chronic renal failure, the findings of this study, could be explained by the three concepts of "Theory of Human Becoming," the first structure could be explained with values, the second with revealing-concealing, and the third with transforming.

• 혜화의학회지
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• 제19권1호
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• pp.99-109
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• 2010

Oriental medicine which is based on internal activeness has constantly been developed for over thousands years and during that time it has encountered Western medicine that has the different system. Because of a lot of different viewpoints of how to understand human and nature, there exist lots of problems to cope with for the integration of two medicines as follows: (1) Oriental medicine regards each person as a cosmos in miniature but Western medicine is based on dualism, substantialism and mechanism. (2) Oriental medicine thinks much of totality as a cosmos in a miniature but Western medicine investigates individual structure and function of human organ and system. (3) Oriental medicine considers that organs are the core of the Oriental medical physiology and makes much account of their functions and their relationships with fundamental texture, but Western medicine gives much emphasis on the structure than functions. Despite of these differences, Oriental medicine and Western medicine should develop mutual understanding and this will facilitate the achievement of the third medicine that is suitable for the primary object of medicine that is to enhance human health and cure diseases.

• 사상체질의학회지
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• 제16권2호
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• pp.32-43
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• 2004

1. Objectives This paper was tried to find out Dong-Mu's recognition systemfor Sasang Structure about "Dongyi Suse Bowon" and "Gyukchigo" 2. Methods The study was researched through comparative studying in a structural manner between "Gyukchigo" 's Affairs-Mind-Body-Objects(事心身物) and "Dongyi Suse Bowon" 's Heaven-Human-Nature-Order(天人性命). 3. Results and Conculusions "Gyukchigo" 's Affairs-Mind-Body-Objects(事心身物) should be understood as Dong-Mu's change process of thinking scheme before writing the book of "Dongyi Suse Bowon" and it is somewhat difficult to make a connected attachment in a lump between "Dongyi Suse Bowon" 's Heaven-Human-Nature-Order(天人性命) and "Gyukchigo" 's Affairs-Mind-Body-Objects(事心身物). Just "Dongyi Suse Bowon" 's Heaven-Human-Nature-Order(天人性命) can be understood as a new recognition systemto encompass" Gyukchigo" 's Affairs-Mind-Body-Objects(事心身物).

• 한국지진공학회논문집
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• 제9권4호
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• pp.19-28
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• 2005

대공간의 쇼핑몰, 오피스 등과 같이 낮은 고유진동수를 가지는 구조물들은 사람의 활동에 의하여 큰 동적응답이 발생할 수 있다. 이러한 활동은 한 사람의 경우보다는 여러 사람에 의한 경우가 더 일반적이다. 본 연구의 목적은 구조물의 모드형상을 이용하여 보행자 전용 구조물과 같이 무리하중을 받는 구조물의 응답을 간단하게 평가하는 것이다. 이를 위하여 사람간의 상관관계가 고려된 무리하중을 받는 구조물의 응답에 관한 식을 유도하였다. 그리고 무리하중을 구성하는 개개의 하중의 연관성을 파악하기 위하여 두 개의 로드셀을 이용하여 하중간의 상관관계를 조사하였다. 구조물의 모드와 하중간의 상관관계에 대한 식을 이용하여 무리하중에 의한 응답을 구조물의 모드형상만으로 평가하는 방법을 제시하였다. 이 방법의 효율성을 확인하기 위하여 보와 바닥판의 예를 들어 무리하중을 동조시킨 경우와 그렇지 않은 경우에 대하여 응답을 평가하였으며 마지막으로 실제 사용중인 구조물을 대상으로 무리하중을 동조시킨 경우와 그렇지 않은 경우에 대하여 응답을 계측하고 이를 제시한 방법과 비교하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.535-538
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• 2000

Human leptin is a 16 kDa (146 amino acids) protein secreted from adipocytes and influences body weight homeostasis. In this report, active human leptin was successfully produced in the culture medium of Bacillus subtilis. After simple purification steps consisting of ammonium sulfate precipitation and anion-exchange column chromatography, 2.3 mg of leptin with a purity of greater than 95% was obtained from the 0.5 L culture with the recovery yield of 54.9%. The purified leptin showed the correct folding structure with one disulfide bond.