• Tuberculosis and Respiratory Diseases
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• 제42권5호
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• pp.777-780
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• 1995

살모넬라의 위장관 감염은 잘알려져 있으나 국소감염은 흔하지 않다. 저자들은 폐암으로 3차례의 항암제 치료을 받던 환자의 총상부위에서 농양이 발생되어 배양결과 salmonella group D가 동정된 1예를 경험하여 문헌과 함께 보고하는 바이다.

• 한국미생물·생명공학회지
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• 제10권4호
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• pp.289-295
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• 1982

Streptomyces bobili (YS-40) 의 Hg, Ag, penicillin-G, ampicillin, chloramphenicol, oxytetracycline, streptomycin, kanamycin에 대한 생육최소저해농도는 각각 15, 10, >3,000, >100, >1,000. >100. <5, <5 $\mu\textrm{g}$/$m\ell$였으며 본 균주의 R-plasmid를 제거시키기 위하여 ethidium bromide, acriflavine, sodium dodecyl sulfate 등의 curing agent를 처리시킨 결과를 요약하면 다음과 같다. Ethidium bromide를 10$\mu\textrm{g}$/$m\ell$의 농도로 처리했을 때 98.0% 정도의 R-plasmid를 제거시킬 수 있었다. pH 7.0에서 curing시킴으로서 R-plasmid가 가장 잘 제거되었으며 분균을 24시간동안 배양해서 curing시킴으로서 R-plasmid의 제거율이 가장 높게 나타났다. Ethidium bromide에 의해서 R-plasmid가 제거된 균과 원균을 여러가지 색소생성배지에서 배양시켜 배양상의 특성을 조사해 본 결과 peptone-beef extract agar 배지에서 aerial mass color가 greyish pink에서 grey로 변했으며 tryptone-yeast extract broth에서 배지에서 soluble pigment가 pale brown에서 무색으로 변했다. 이 결과로는 aerial mycelium, melanin 생성과 R-plasmid 는 무관하다고 추측된다.

• 한국환경농학회지
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• 제27권1호
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• pp.50-59
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• 2008

A greenhouse experiment was conducted to investigate the role of the arbuscular mycorrhizal(AM) fungus, Glomus mosseae(BEG 107) in enhancing growth and arsenic(As) and phosphorus(P) uptake of white clover(Trifolium repens) and evening primrose(Oenothera odorata) in soil collected from a gold mine having concentrations of 381.6 mg total As $kg^{-1}$ and 20.5 mg available As $kg^{-1}$. Trifolium repens and O. odorata are widely distributed on abandoned metalliferous mines in Korea. The percent root colonization by the AM fungus was 55.9% and 62.3% in T. repens and O. odorata, respectively, whereas no root colonization was detected in control plants grown in a sterile medium. The shoot dry weight of T. repens and O. odorata was increased by 323 and 117% in the AM plants compared to non-mycorrhizal(NAM) plants, respectively. The root dry weight increased up to 24% in T. repens and 70% in O. odorata following AM colonization compared to control plants. Mycorrhizal colonization increased the accumulation of As in the root tissues of T. repens and O. odorata by 99.7 and 91.7% compared to the NAM plants, respectively. The total uptake of P following AM colonization increased by 50% in T. repens and 70% in O. odorata, whereas the P concentration was higher in NAM plants than in the AM plants. Colonization with AM fungi increased the As resistance of the host plants to As toxicity by augmenting the yield of dry matter and increasing the total P uptake. Hence, the application of an AM fungus can effectively improve the phytoremediation capability of T. repens and O. odorata in As-contaminated soil.

• 한국균학회지
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• 제38권2호
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• pp.89-94
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• 2010

농업경영측면에서, 또 균학적생화학적 측면에서도 식물을 침해하는 진균들의 연구는 반드시 필요하며 병 발생이나 저항성 발현 기작 구명은 기주와 기생체에 대한 연구를 동시에 진행해야 정확히 파악할 수 있다. 현재 병원균이 생산하는 분비체상과 비병원성 인자에 대한 연구는 많은 경우 세균에서 수행되고 있으며 사상균 중 조균인 Phytophthora와 진균인 Cladosporium에서만 병원균의 effector 복합체와 기주의 저항성 기제 간 관계가 같이 진행되고 있을 뿐이다. 앞에서 살펴보았듯 진균-기주 체계에서 단백질 분해가 병원성 조절 및 침입에 관여한다고 정확히 알려진 것은 단지 수종에 불과하며 그 기작도 세포자가포식과 ubiquitin 부가반응에 제한되어 있다. Post translational modification과 단백질 분해기작이 대단히 다양하고 거의 모든 진핵생물 체계에서 관찰되고 있음을 고려할 때 단백질 분해 과정은 세균 뿐 아니라 진균에서도 병원성 발현과 저항성 조절에 참여하고 있을 것으로 생각되며 이에 대한 연구가 앞으로 계속 요구될 것이라 생각된다.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.394-399
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• 2010

국내의 GM식물개발과 관련된 R&D사업 중 대표적이라고 할 수 있는 "바이오그린21사업"과 작물유전체기능연구사업단에서 지난 2007년부터 2009년 말 현재까지의 사업성과 중 논문과 동록 된 특허를 분석하여 본 결과 두개의 사업단을 통하여 총 1,212건의 SCI논문과 451건의 비 SCI논문, 그리고 348건의 특허를 등록한 것으로 조사되었다. 이중에서 바이오그린21사업단에서는 6.0%의 SCI 논문, 10.2%의 비SCI논문, 12.6%의 등록된 특허가 그리고 작물유전체기능연사업단의 경우에는 15.7%의 SCI논문, 21.1%의 비SCI 논문, 81.6%의 등록된 특허가 GM식물의 개발과 직접적으로 관련된 것으로 조사되었다. 개발하고자 하는 GM식물의 농업적 특성은 생물학적 스트레스 또는 무생물적 스트레스에 저항성을 갖는 작물 특히 벼와 고추가 가장 많았으나 포장시험까지 진행된 연구 성과는 아직 극히 저조한 것으로 밝혀졌다. 이러한 조사 결과는 국내에서 현재 실험실 또는 온실 단계에서 검정된 초기 단계의 GM식물의 조기상용화를 위하여 보다 많은 연구비가 투자되어야 할 것을 시사하였다.

• Journal of Plant Biotechnology
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• 제41권1호
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• pp.1-9
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• 2014

So far it has been generally considered that proteomic approaches are very useful for studying plant-microbes interaction. In this review, recent studies based on papers published from 2010 to 2013 have investigated proteomics analysis in various interaction during plant-fungal pathogen infection by means of gel-based proteomics coupled with mass spectrometry (MS)-based analysis. In rice, three papers focused on rice-Magnaporthe oryzae interaction were mainly reviewed in this study. Interestingly, another study showed proteomic changes in rice inoculated with Puccinia triticina, which is not only an fungal pathogen in wheat and but also results to the disease resistance with non-host defense manner in rice. Additionally, proteomics analysis has been widely subjected to understand defense mechanism during other crops (wheat, tomato, strawberry and mint) and their fungal pathogen interaction. Crops inoculated are analyzed to identify differentially regulated proteins at various tissues such as leaf and apoplast using 2-DE analysis coupled with various MS approaches such as MALDI-TOF MS, nESI-LC-MS/MS and MudPIT, respectively. Taken together, this review article shows that proteomics is applicable to various organisms to understand plant-fungal pathogen interaction and will contribute to provide important information for crop disease diagnosis and crop protection.

• 식물병연구
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• 제25권1호
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• pp.1-7
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• 2019

벼도열병균은 벼를 재배하는 모든 지역에서 경제적으로 매우 중요한 병이다. 또한, 벼도열병균은 기주인 벼와 유전자 대유전자설이 적용되는 대표적인 식물병원균 모델이다. 재배지에 도입된 새로운 저항성 벼 품종의 빠른 저항성 상실은 병원균 집단의 레이스 변이가 주요 메커니즘으로 제안되고 있다. 이러한 새로운 레이스 변이는 저항성 유전자에 대항하는 비병원성 유전자의 변이에 의해 나타날 수 있는데, (i) 점돌연변이, (ii) 전이인자(transposon)의 삽입, (iii) frame shift등이 그 대표적인 예라고 할 수 있다. 비병원성 유전자 AVR-Pita1은 이러한 다양한 변이의 원인들이 모두 보고된 대표적인 비병원성 유전자이다. 이 총설에서는 비병원성 유전자 AVR-Pita1에 관한 다양한 정보를 제시하고, 상동성 유전자들인 AVR-Pita2 및 AVR-Pita3 유전자를 정리하였다. 이와 함께, 변이의 원인이 되는 다양한 예제를 리뷰 하였다.

• The Plant Pathology Journal
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• 제35권3호
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• pp.257-273
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• 2019

Tomato (Solanum lycopersicum) is one of the most widely grown and economically important vegetable crops in the world. Tomato chlorosis virus (ToCV) is one of the recently emerged viruses of tomato distributed worldwide. ToCV-tomato interaction was investigated at the molecular level for determining changes in the expression of tomato genes in response to ToCV infection in this study. A cDNA library enriched with genes induced in response to ToCV infection were constructed and 240 cDNAs were sequenced from this library. The macroarray analysis of 108 cDNAs revealed that the expression of 92 non-redundant tomato genes was induced by 1.5-fold or greater in response to ToCV infection. The majority of ToCV-induced genes identified in this study were associated with a variety of cellular functions including transcription, defense and defense signaling, metabolism, energy, transport facilitation, protein synthesis and fate and cellular biogenesis. Twenty ToCV-induced genes from different functional groups were selected and induction of 19 of these genes in response to ToCV infection was validated by RT-qPCR assay. Finally, the expression of 6 selected genes was analyzed in different stages of ToCV infection from 0 to 45 dpi. While the expression of three of these genes was only induced by ToCV infection, others were induced both by ToCV infection and wounding. The result showed that ToCV induced the basic defense response and activated the defense signaling in tomato plants at different stages of the infection. Functions of these defense related genes and their potential roles in disease development and resistance to ToCV are also discussed.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1430-1437
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• 2021

Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD₅₄₀ = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.

• The Plant Pathology Journal
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• 제37권2호
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• pp.152-161
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• 2021

Sugar beet (Beta vulgaris L.) is known as a key product for agriculture in several countries across the world. Beet soil-borne virus (BSBV) triggers substantial economic damages to sugar beet by reducing the quantity of the yield and quality of the beet sugars. We conducted the present study to report the complete genome sequences of two BSBV isolates in Turkey for the first time. The genome organization was identical to those previously established BSBV isolates. The tripartite genome of BSBV-TR1 and -TR3 comprised a 5,835-nucleotide (nt) RNA1, a 3,454-nt RNA2, and a 3,005-nt RNA3 segment. According to sequence identity analyses, Turkish isolates were most closely related to the BSBV isolate reported from Iran (97.83-98.77% nt identity). The BSBV isolates worldwide (n = 9) were phylogenetically classified into five (RNA-coat protein read through gene [CPRT], TGB1, and TGB2 segments), four (RNA-rep), or three (TGB3) lineages. In genetic analysis, the TGB3 revealed more genetic variability (Pi = 0.034) compared with other regions. Population selection analysis revealed that most of the codons were generally under negative selection or neutral evolution in the BSBV isolates studied. However, positive selection was detected at codon 135 in the TGB1, which could be an adaptation in order to facilitate the movement and overcome the host plant resistance genes. We expect that the information on genome properties and genetic variability of BSBV, particularly in TGB3, TGB1, and CPRT genes, assist in developing effective control measures in order to prevent severe losses and make amendments in management strategies.