• Journal of Dairy Science and Biotechnology
• /
• 제42권2호
• /
• pp.48-63
• /
• 2024

유산균이 가지고 있는 𝛽-glucosidase활성이 가시오갈피(A. senticosus)의 주요 활성 성분인 EE와 EB와 같은 식물성 화합물을 분해하여 syringaresinol(SYR)로 전환하는 역할을 하는지 탐구하였다. 김치 등에서 분리배양한 유산균 125개 중, 𝛽-glucosidase 활성을 지닌 균주를 세포 외 및 내부에서 스크리닝된 것이 46종으로 확인되었으며, 특히 L. curvatus인 LFFR 20-011과 L. brevis인 LFFR 20-043 등이 가시오갈피 발효를 통해 SYR의 생산을 2배 이상 함량을 증가시키는 것을 확인할 수 있었다. 추가적으로, 본 연구는 SYR이 조골세포 분화에 미치는 영향을 조사하였다. SYR처리는 분화를 유도하고 조골세포 분화 초기 마커인 Runx2, Type I COL, 및 성숙 마커인 osteocalcin(OCN)의 mRNA 발현 수준을 유의미하게 증가시켰다. 이러한 결과는 생물전환기술로 증가된 한국가시오갈피의 SYR의 존재가 뼈의 건강과 성장에 긍정적인 영향을 줄 수 있는 잠재력을 가지고 있음을 시사하며, 향후 고령시대의 근골격계 건강 증진에 관한 연구로의 가능성을 제시하여, 한국가시오갈피의 유산균 발효물에 대한 건강기능식품으로써의 기초적인 자료를 제공한다.

• 한국자원식물학회지
• /
• 제34권6호
• /
• pp.575-599
• /
• 2021

The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.

• 대한본초학회지
• /
• 제30권2호
• /
• pp.19-24
• /
• 2015

Objectives : The experiment was performed to investigate promotive effects of haeae-tang (HET) extract, a traditional Korean medicinal recipe, on hair growth, protein and gene expression in hair-removed C57BL/6. Methods : In experiment, animals were divided into 3 groups including normal (vehicle), HET ethanol extract and 5% minoxidil-treated groups (Minoxidil, positive control). The vehicle or testing samples were daily treated with 0.2ml per on hair-shaved dorsal skin of C57BL/6mice for 15 days. Effects of testing samples on hair growth was monitored through phototrichogram analysis by folliscope on the initial, $5^{th}$, $10^{th}$, $15^{th}$ day, respectively. Also, gene and protein expressions of vascular endothelial growth factor (VEGF) and Insulin like growth factor-1 (IGF-1), relevant to hair growth, were examined. Results : Hair density and hair thickness of Minoxidil treated-group was significantly increased compared to that of vehicle application on the $15^{th}$day, respectively. Dorsal hair density of HET treated-group was significantly increased compared to that of vehicle application on the $15^{th}$day. In addition, the Minoxidil group significantly increased the expression of cutaneous IGF-1 protein and mRNA compared to that of the vehicle-applied group on the $15^{th}$ day. And HET-treated group significantly increased the expression of dorsal VEGF protein compared to that of the vehicle-applied group on the $15^{th}$ day. Conclusions : These results suggest that this Korean medicinal recipe, HET has promoting activity on hair growth in an Alopecia animal model thus it can be used as a material of agent or products for improvement or prevention of alopecia.

• Journal of Animal Science and Technology
• /
• 제64권3호
• /
• pp.409-431
• /
• 2022

Various insects have emerged as novel feed resources due to their economical, eco-friendly, and nutritive characteristics. Fish, poultry, and pigs are livestock that can feed on insects. The digestibility of insect-containing meals were presented by the species, life stage, nutritional component, and processing methods. Several studies have shown a reduced apparent digestibility coefficient (ADC) when insects were supplied as a replacement for commercial meals related to chitin. Although the expression of chitinase mRNA was present in several livestock, indigestible components in insects, such as chitin or fiber, could be a reason for the reduced ADC. However, various components can positively affect livestock health. Although the bio-functional properties of these components have been verified in vitro, they show positive health-promoting effects owing to their functional expression when directly applied to animal diets. Changes in the intestinal microbiota of animals, enhancement of immunity, and enhancement of antibacterial activity were confirmed as positive effects that can be obtained through insect diets. However, there are some issues with the safety of insects as feed. To increase the utility of insects as feed, microbial hazards, chemical hazards, and allergens should be regulated. The European Union, North America, East Asia, Australia, and Nigeria have established regulations regarding insect feed, which could enhance the utility of insects as novel feed resources for the future.

• 한국버섯학회지
• /
• 제20권1호
• /
• pp.13-21
• /
• 2022

Cordyceps militaris, a well-known traditional Chinese medicine, has multiple health-promoting effects. It is used as a herbal remedy and health food in Asian countries. Cultured mycelia are often used as a substitute for natural C. militaris. In the present study, the mycelia and fruiting bodies of artificially cultured C. militaris were analyzed using a metabolomics approach. The protein and crude fat contents of the mycelia were substantially higher than those of the fruiting bodies. The top three abundant amino acids in the mycelia were proline (3.9 g/100 g), aspartic acid (2.9 g/100 g), and glutamic acid (2.7 g/100 g). The carbohydrate content was similar in the fruiting bodies and mycelia. Analysis revealed that both the fruiting bodies and mycelia are rich in phenolic compounds and exhibit antioxidant activity. Further, six metabolites were significantly different between the mycelia and fruiting bodies. The levels of Ca, glucose, Mg, and Se were higher in the mycelia than in the fruiting bodies. In contrast, mannitol and Zn were more abundant in the fruiting bodies. The current study provides a comprehensive metabolic profile of the mycelia and fruiting bodies of artificially cultured C. militaris. Such an exercise is potentially important for understanding the metabolism of C. militaris and facilitating the use of cultured mycelia as a supplement to C. militaris fruiting bodies in traditional Chinese medicine.

• 한국체육학회지인문사회과학편
• /
• 제55권5호
• /
• pp.619-633
• /
• 2016

지난 2015년 3월 27일 공포된 체육단체의 통합은 대한민국이 "스포츠강국"에서 "스포츠선진국"으로 발돋움 할 수 있는 계기를 마련한 국내 체육계의 노력의 결실이며, 스포츠계 새로운 패러다임의 도입이다. 이에 본 논문은 체육단체의 성공적인 통합을 위한 스포츠시스템과 프로그램 측면 토대의 마련을 위해, 지난 2012년부터 캐나다의 체육단체 통합과 스포츠시스템의 운영에 발판이 된 Long-Term Athlete Development(LTAD) 프레임워크를 비판적으로 살펴 본 후, 국내 활용도에 대해서 논의해보고자 한다. 또한, 이를 바탕으로 국내에 적합한 통합모델의 제시와 운영전략을 제시하였다. 본 논문에서 제시한 "한국형 스포츠시스템 통합모델"은 우리나라 전 국민을 대상으로 연령·발육발달단계·스포츠 입문 시기 별 스포츠 참여의 틀을 제공한다. 이는 지난 10년 간 체육계의 쟁점이었던 학교체육·엘리트체육·생활체육의 연계는 물론 엘리트선수의 발굴과 육성, 그리고 평생체육으로의 연계까지 가능하게 할 것으로 생각된다. "한국형 스포츠시스템 통합모델"은 앞으로 실제에의 적용, 평가, 그리고 수정의 과정을 거쳐 지속적으로 진화해 나아가야 할 것이다.

• Journal of Dairy Science and Biotechnology
• /
• 제38권2호
• /
• pp.89-98
• /
• 2020

본 연구의 목적은 한국 전통 음식 김치에서 분리한 유산균의 특성을 연구하기 위해 형태학적, 생화학적 특성을 조사하였다. 한국의 전통 발효 식품에서 젖산균을 확인하기 위해 분리된 균주의 그람염색을 수행한 후 Macrogen에서 16S rRNA 분석 결과, DKGF9(Lactobacillus plantarum), DKGF1(Lactobacillus paracasei ), DKGF8(Lactobacillus casei ), DK207(Lactobacillus casei ), DK211(Lactobacillus casei )이 확인되었다. 우리는 한국의 전통 발효 식품인 김치에서 분리된 5가지 LAB의 기본 생물학적 활성에 대한 실험을 수행했다. 37℃, 55℃, 65℃, 75℃에서 각각 5분, 15분 5균주의 내열성 확인 결과, 상업 균주인 Lactobacillus acidophilus LA-5의 내열성과 유사하거나 더 높음을 보여주었다. 장내부착능에서는 선발균주 모두 상용균주와 비교했을 때 10⁷ CFU/mL 이상으로 우수한 결합능을 보여주었고, KCTC(한국생명공학연구원 생물자원센터)에서 분양받은 Escherichia coli KCTC1682, Salmonella enterica KCTC2054, Bacillus cereus KCTC3624 3종을 활용한 항균활성 결과, 모든 균주는 상업용 균주인 L. acidophilus LA-5와 비교하여 유사하거나 더 높은 항균 활성을 나타냈다. 단백질분해능력 실험에서, 5개의 균주는 clear-zone의 직경이 24시간에서 72시간으로 갈수록 점차 증가하고, L. paracasei DKGF1이 가장 큰 직경을 갖고 있어 단백질분해능력이 가장 우수한 것으로 나타났다. 5개의 균주로부터 선택된 3개의 균주는 ABTS, DPPH, FRAP, Hydroxyl radical scanenging 활성을 포함하여 다양한 항산화활성 효과를 나타냈다. 결과적으로, 5가지 균주 중에서 우수한 기능성을 갖는 L. paracasei DKGF1이 잠재적인 프로바이오틱스 활성을 나타내며, 건강 관련 제품의 개발에 유용한 균주라고 판단된다.

• 동의생리병리학회지
• /
• 제31권6호
• /
• pp.374-379
• /
• 2017

Hair loss affects interpersonal relationships and causes psychological stress. In this study, we investigated the antioxidant activity of Cynanchi Wilfordii Radix (CWR) and its effects on dermal papilla (DP) cells. Antioxidant efficacy was examined by ABTS assay. To confirm the effect on cell activity, MTS assay was performed and cell count was directly measured by hemocytometer. The mRNA expression of genes involved in hair formation and hair loss formation was confirmed by quantitative RT-PCR. CWR has a strong antioxidant activity. Cell viability of DP cells was increased to 118.5% by treatment of 0.5 mg/ml CWR for 24 hours, but the effect on the cell number was insignificant. These results suggest that CWR increases mitochondrial activity without promoting cell proliferation. Treatment of DP cells with 0.5 mg/ml CWR resulted in 48.5% reduction of mRNA expression of type 2 $5{\alpha}$-reductase, a major cause of male hair loss. In addition, mRNA expression of bone morphogenetic pretein (BMP), fibroblast growth factor (FGF)7, and FGF10, which are closely related to hair growth, was also decreased. Reactive oxygen species (ROS) acts as a cause of hair loss. The excellent antioxidant efficacy of CWR is thought to be able to effectively remove ROS. The dihydrotestosterone produced by type 2 $5{\alpha}$-reductase in DP cells is a potent inducer of male pattern hair loss. The inhibitory effect of type 2 $5{\alpha}$-reductase mRNA on DP cells induced by CWR may induce a positive therapeutic effect of male pattern hair loss.

• Journal of Nutrition and Health
• /
• 제55권6호
• /
• pp.617-629
• /
• 2022

본 연구는 속단으로부터 단일 화합물로 분리한 Hed의 조골세포 활성 효능과 작용 기전 규명을 위해 수행되었다. Hed는 MC3T3-E1 세포의 증식과 ALP 효소 활성을 자극하여 세포외 기질에 인산과 칼슘 이온 침착을 증가시켰다. Hed는 BMP-2/Smad 신호 전달 경로를 활성화하여 Runx2, ALP, OPN 및 ProCOL의 mRNA와 단백질 발현을 유의하게 증가시켜 조골세포의 성숙과 분화를 유도하였다. 따라서 본 연구결과 Hed는 조골세포 활성 효능을 가진 것으로 판단되며 항골다공증 기능성 소재로의 개발 가능성을 확인하였다.

• 보건교육건강증진학회지
• /
• 제11권2호
• /
• pp.87-106
• /
• 1994

Owing to the use of many cultural facilities, the elevation of life standards and the high level progress of industry, visual impairment was on increasing trends. Especially due to studying for many hours, the myopia of adolescents became a serious social issue. The purpose of this study was to understand adolescent's wearing glasses and eyesight condition, whether the primary myopia factor and the subjective symptoms were different between the myopia group and the normal group, between the wearing glasses group and the non-wearing glasses group, and the effect on physical, mental and study activity of myopia. The subject of investigation was 627 middle school students in Seoul and the investigation was accomplished from July 7th, 1993 to July 12th, 1993. The data were analyzed by the percentage, x²-test, t-test and ANOVA of SPSS. The results were as followed; 1. The rate of the right and left eyesights higher than 0.8 in the normal group was 47.0%, and those less than 0.7 in the myopia group was 53.0%. In the boy students, the normal group was 54.1%, the myopia group was 45.9%. In the girl students, the normal group was 39.2%, the myopia group was 60.8%. Therefore the girl students' myopia rates were higher than the boy students', and this result was significant(p<0.01). 2. The rate of wearing glasses of the middle school students was 44.6%. The rate of wearing glasses of the girl students was 56.8%. The rate of wearing glasses of the girl students was higher than that of the boy students 43.2%. The rate of necessary glasses of the middle school students was 6.1% and the rate of necessary glasses of the boy students was 7.9%, the rate of necessary glasses of the girl students was 4.1%. 3. In case of a family member of the student wear glasses, the rate of wearing glasses was higher. 4. In the myopia group, the main reason for not wearing glasses was "Uncomfortable"(63.3% in the boy students, 40% in the girl students). In case of the girl students, "Nonpermission of their parents" was 18.7% and remarkably higher than 5.1% in case of the boy students. 5. The factor of myopia was that "The bad attitude of watching TV closely" was 19.9%, that "The dimly-lit room" was 6.6%. 6. In order to protect eyesight, the rate of the students who practiced "Looking at something from afar 3∼4times a day" was 37.3%, the rate of the students who did "Eye exercise" was 17.5%, the rate of the students who took "A medicine for promoting nutrition" was 12.9%. The rate of taking the medicine was higher than 3.5% in the normal eyesight group. 7. After near working, the point of subjective symptoms was higher in the myopia group than in the normal group and in the wearing glasses group than in the unwearing group and in the girl students than in the boy students. 8. The longer time to watch TV, the higher point of subjective symptoms. The longer distance to watch TV, the lower point of subjective symptoms(p<0.05).