• 치과방사선
• /
• 제24권2호
• /
• pp.249-260
• /
• 1994

The purpose of this study was to aid in the radiation therapy of head and neck cancer patients. For this study, radiation survival curves were generated for Bl6, MG-63 and YAC-l cell lines using semiautomated MTT assay and Dye Exclusion Assay. Irradiation of 2, 4, 6, 8, 10Gy were delivered at room temperature at a dose rate of 210.2cGy/min using / sup 60/Co γ-ray Irradiator ALOORAOO 8. The viable cells were determined for each radiation dose and compared to control values. The obtained results were as follows: 1. There was significantly different absorbance at 10Gy on B16 cell line in MTT assay(P<0.05). 2. There was significantly different absorbance at 4, 6, 8, 10Gy on MG-63 cell line in MTT assay(P<0.05). 3. YAC-l cell line was more sensitive than B16 or MG-63 cell line to all doses of radiation(P<0.05). 4. There was significantly different absorbance among all tumor cell lines except between B16 and MG-63 cell line at ZGy in MTT assay(P<0.05). 5. Good correlation was obtained between MTT assay and DEA(P<0.05). The efficient of correlation of B16, MG-63 and YAC-l cell line was 0.845, 0.824 and 0.906, respectively.

• 대한기관식도과학회지
• /
• 제4권2호
• /
• pp.159-164
• /
• 1998

The developmental and subsequent addition of laser technology to existing surgical techniques offered new and exciting Possibilities for improving traditional endoscopic operations and expanding the scope of the specialty of otolaryngology-head and neck surgery. Lasers were found to be Precise, but potentially dangerous, surgical instruments whose use was associated with certain distinct and unprecedented advantages, but also with many unique and potentially serious, and sometimes catastrophic complications. The Purpose of this study was to evaluate the of elation technique of $CO_2$laser, kinds of anesthesia, equipments of operating room, and education of medical staff. Four hundreds 4 cases of $CO_2$laser laryngeal microsurgery were reviewed for the safety of $CO_2$laser in laryngeal microsurgery. Vocal polyp (46,3%) was the most common pathology in our cases. The others were vocal nodule, Reinke`s edema, epiglottic cyst, laryngeal granuloma, laryngeal papilloma, intracordal cysts, laryngeal tuberculosis, laryngeal web, laryngotracheal stenosis, and laryngeal cancer. Following complete equipment controls, treatment area controls, maintenance, service and procedural controls, personel protective equipment, and warning signs, no complications were found in our cases. In conclusion, laryngeal microsurgery with $CO_2$laser could be done safely without complication if complete preoperative preparation and education of medical staff preceded.

• 대한두개안면성형외과학회지
• /
• 제17권3호
• /
• pp.176-179
• /
• 2016

Primary cutaneous mucinous carcinoma (PCMC) is a rare low-grade malignant neoplasm derived from the eccrine glands. PCMC most commonly arises in the head and neck, with the eyelid being the most common site of origin. This case report describes a 51-year-old male with a painless, pigmented superficial nodular lesion over his right lower eyelid. The lesion was considered to be benign, and the initial treatment was simple excision with a 3-mm margin. However, histologic examination revealed the diagnosis of PCMC, and the patient underwent re-excision of the tumor site with an additional 3-mm margin from the initial scar. Histologic study of this second margin was free of any malignant cells. The patient experienced no postoperative complication or recurrence after 2 years. In our case, the skin lesion had benign morphologic findings and was strongly suspected to be a benign mass. Physicians should be aware of this tumor and be able to differentiate it from benign cystic or solid eyelid lesions.

• International Journal of Oral Biology
• /
• 제49권1호
• /
• pp.18-25
• /
• 2024

Despite advancements in therapeutic approaches, radiotherapy and cisplatin-based chemotherapy remain primary noninvasive treatments for patients with oral squamous cell carcinoma (OSCC). Moreover, the 5-year survival rate for patients with OSCC has remained almost unchanged for several decades, and many side effects of chemotherapy still exist. In this study, three-dimensional (3D) models of OSCC were established using fibroblasts, and the efficacy of various biological inhibitors was evaluated. A culture of epithelial cells with two types of fibroblasts (hTERT-hNOFs and cancer-associated fibroblasts) within a type I collagen matrix resulted in the formation of a continuous layer of tightly packed cells compared to models without fibroblasts. Furthermore, the effects of biological chemicals, including Y27632, latrunculin A, and verteporfin, on these models were investigated. The stratified formation of the epithelial layer and invasion in OSCC 3D-culture models were effectively inhibited by verteporfin, whereas invasion was weakly inhibited by Y27632 and latrunculin. Collectively, the developed OSCC 3D-culture models established with fibroblasts demonstrated the potential for drug screening, with verteporfin showing promising efficacy.

• 대한방사선치료학회지
• /
• 제20권2호
• /
• pp.83-89
• /
• 2008

목 적: 두경부 암 환자 치료 시 표적 및 주변 장기(Target 및 Structure)를 확인하는 방법으로 MVCBCT, KVCBCT, CT On-rail System, Ultrasound 등이 이용된다. 이 중 MVCBCT의 경우는 뼈를 이용한 표적 및 주변 장기의 확인에는 유용하나, 연부조직으로 구성된 표적 및 주변 장기의 확인에는 어려움이 있다. 이에 본 연구에서는 CT On-rail System을 이용하여 두경부 암 환자의 이하선의 움직임에 대한 파악과 체적의 변화에 따른 선량의 변화를 연구해 보고자 한다. 대상 및 방법: 본원에서 두경부 암으로 치료받는 환자 5명을 대상으로 하였다. 선택된 환자는 시멘스사 ARTISTE CT Vision (CT On-rail System)을 이용하여 주당 3회씩 CT 스캔을 시행하였다. CT 스캔 후 이하선의 움직임을 치료 계획시의 영상과 비교하여 이동된 좌표를 얻었으며, 이하선의 체적을 확인하기 위하여 획득한 CT영상을 치료계획 시스템(RTP System)에 전송하여 변화된 체적에 따른 선량분포의 차이를 확인하였다. 결 과: 이하선의 움직임에 의한 표적 좌표의 변화는 X: -0.4~0.4 cm, Y: -0.4~0.3 cm, Z: -0.3~0.3 cm이었다. GTV의 체적은 평균 7.11%/week로 감소되었으며 이하선의 체적과 선량의 변화는 평균적으로 좌측 이하선의 체적은 평균 4.81%/week, 우측 이하선의 체적은 2.91%/week, 선량은 좌측 이하선의 선량은 3.66%/week, 우측 이하선 선량은 2.01%/week 감소하였다. 결 론: CT On-rail System에서 얻어진 영상을 이용하여 표적 및 주변 장기를 확인한 결과 MVCBCT를 이용한 영상에 비해 연부조직의 관찰에 우수함을 알 수 있었다. 또한, 획득한 영상으로 재 치료계획(Replanning)을 한 후 선량의 재분포를 획득한 결과 단순한 움직임의 보정뿐 아니라 체적의 변화를 고려한 정확한 선량분포를 얻을 수 있었으며, 이렇게 보정된 선량의 전달은 Adaptive Targeting Radiotherapy가 가능할 수 있다고 사료된다. 향후 선량의 전달까지 걸리는 시간을 단축할 수 있는 Real Time Adaptive Targeting Radiotherapy가 구축되어야 할 것으로 사료된다.

• 대한후두음성언어의학회지
• /
• 제27권2호
• /
• pp.122-125
• /
• 2016

Although many factors associated with difficult intubation have been known, predictors of difficult mask ventilation are not well known. We experienced a case of nearly complete airway obstruction following usual anesthetic induction which needed various emergency treatments. The patient had a preoperative diagnosis of contact granuloma of right posterior vocal cord and bilateral vocal cord palsy but later was found out as invasive laryngeal cancer. Upon the surgical field of view, both vocal cords were showing significantly thickened and fixated appearance and was considered as in the critical narrowing state with the potential of complete obstruction. Using $C-MAC^{(R)}$ video laryngoscope we were able to see the narrowed vocal cord and choose proper size of endo-tracheal tube. Consequently, intubation was successfully done and operation was conducted. From this case, we have lessons that physicians should examine the patient's airway more carefully in case of laryngeal mass and prepare emergency measures.

• Radiation Oncology Journal
• /
• 제15권1호
• /
• pp.19-26
• /
• 1997

목적 : 근치적 방사선치료 후 국소 재발된 비인강암의 방사선 재치료의 방법으로서 정위적 방사선 분할치료를 적용하여 그 치료 효과를 보고자 한다. 대상 및 방법 : 1995년 9월부터 1996년 8월까지 삼성 서울 병원 치료방사선과에서 정위적 방사선 분할치료를 시행받은 3명의 국소 재발 비인강암 환자들을 대상으로 하였다. 2명은 정위적 방사선 분할치료의 적용이 세 번째 방사선치료였다. 2명은 방사선 민감제로서 항암치료를 함께 시행하였다. 정위적 방사선 분할치료는 환자 개별적으로 제작되어 재장착이 용이한 Gill-Thomas-Cosman(GTC) 정위틀의 좌표계를 기준으로 하였으며 XKnife-3를 이용하여 multiple non-coplanar arc therapy의 치료 계획을 수립하여 일회당 2.5Gy씩 모두 45Gy 또는 50Gy를 조사하였다. 결과 : 3명 모두에서 만족할 만한 자각 증상의 호전을 경험하였으며 치료 종료 후 1개월에 시행한 자기 공명 영상에서 종양 크기의 현저한 감소를 확인하였으며 방사선으로 인한 뇌신경 장애의 부작용은 없었다. 2명은 정위적 방사선 분할치료의 치료 범위 바깥에서 다시 재발하여 7개월 및 9개월에 사망하였으며 1명은 4개월째 생존해 있다. 결론 : 정위적 방사선 분할치료는 국소 재발 비인강암의 방사선 재치료의 방법으로서 비교적 안전하고 효과적인 치료 방법을 확인하였으며 원격 전이에 대한 보다 효과적인 항암치료의 개발이 요구된다.

• 대한임상검사과학회지
• /
• 제36권2호
• /
• pp.269-273
• /
• 2004

Squamous cell carcinoma(SCC) associated antigen is a subfraction of TA-4, a tumor-associated antigen first described by Kato and Torigoe in 1977. TA-4, obtained from squamous cell carcinoma cancer tissue of the uterine cervix, has been characterized as a glycoprotein with a molecular weight of approximately 45,000 daltons. SCC antigen has been studied in other squamous cell malignancies including lung, esophagus, head and neck, anal canal, and skin. SCC antigen is shed naturally through sweat, saliva and other body fluids. Contamination of specimens, tray, bead dispenser or other accessories with sweat, saliva or aerosols can cause falsely elevated values. To reduce the possibility of contamination, gloves should be worn in all phases of assay preparation, and when handling specimens, accessories or reagents that will be used in SCC and Thyroid function test(TFT).

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제28권6호
• /
• pp.434-439
• /
• 2002

Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. OSCC generally has a poor prognosis due to its tendency towards a local invasion and subsequent metastasis, which is mediated by multiple proteolytic enzymes and angiogenesis. Soy products contain high levels of isoflavonoids, including the tyrosine kinase inhibitor, genistein, which has been identified as a potent inhibitor of cell proliferation and in vitro angiogenesis. The purpose of this in vitro study is to evaluate the anti-cancer effect of genistein with respect to the angiogenesis and basement membrane invasion in OSCC. The highly invasive OSCC cell line, HSC-3 cells were cultured in the presence of $10{\mu}M$ genistein for 24h. To evaluate the effects of genistein on the invasiveness and the gelatinolytic activity, in vitro invasion assay and zymography were performed. In order to evaluate the effect on the VEGF and bFGF mRNA expression, RT-PCR and northern hybridization reaction, and chemiluminescence detection were applied. The in vitro invasion assay showed that the genistein treatment reduced the cellular invasion through the artificial basement membrane and significant difference between the control group and the genistein treated group was shown in MMP-2 activity. Especially, the 62 kDa activated form of MMP-2 in the control group was 1.8 times higher than that in the genistein treated group. The results of the northern blot analyses indicated that VEGF mRNA expression in the genistein treated group was significantly down regulated. This study showed that genistein inhibits angiogenesis and reduces basement membrane invasion in OSCC. It seems to support the possibility of genistein as an anti-cancer agent.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권2호
• /
• pp.757-760
• /
• 2015

Aim: To present the epidemiologic data (age, gender, size etc) and histopathologic and immunohistochemical features of cases of rhabdomyosarcoma (RMS) reported in our department. Settings: Section of Histopathology, Department of Pathology and Microbiology, Aga Khan University Hospital, Karachi, Pakistan. Duration: All cases of RMS diagnosed between January 1, 2003 and December 31, 2012 were included in the study. Results: A total of 277 cases were included. Embryonal rhabdomyosarcoma (ERMS) was by far the dominant histologic type (87.4%) followed by alveolar type (ARMS)(9.4%). ERMS was much more common in males (64.0%)and over 65% of cases occurred in the first decade of life (over 90% in the first two decades). Head and neck region was commonest site for ERMS (46.7%), followed by the genitourinary system (16.1%). Over 65% cases of ARMS occurred in the extremities. Over 80% cases of ARMS occurred in the first 3 decades of life. Immunohistochemical staining for Desmin and MyoD1 was positive in 96.7% and 85.4% respectively. Conclusions: The epidemiologic data and microscopic findings in our patients are similar to international published data on rhabdomyosarcoma.