• 대한지역사회영양학회:학술대회논문집
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• 대한지역사회영양학회 2003년도 추계학술대회
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• pp.1048-1048
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• 2003

The purpose of this study was to investigate dietary behaviors, body image, hematological index and nutrient intake of female athletes in Incheon. The subjects were 112 female athletes (field and track: n=32, firing: n=27, fencing: n=29, swimming: n=14, badminton: n=10) from middle and high schools in Incheon. This cross-sectional study was conducted by a self-administered questionnaire. Fasting blood samples were obtained and analyzed for hemoglobin (Hb), hematocrit(Hct), ferritin, serum iron, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), unsaturated iron binding capacity (UBC), total iron binding capacity (TIBC) and transferrin saturation (TS). Nutrient intakes collected from 3 day-recalls were analyzed by the Computer Aided Nutritional Analysis Program. Statistical analysis was conducted using SPSS 10.0 program. The results are summarized as follows: Average age of field and track athletes, firing, fencer and swimmer was 14 years and that of badmintoner was 17 years. Most of female athletes had dietary problems such as unbalanced meals, skipping meals, and preference of processed foods. More than 60% of female athletes skipped breakfast. As for perception of body image, most of female athletes perceived themselves fatter compared to normal body image. Especially, field and track athletes were more significantly experienced weight control compared to the other athletes (p<0.05). As for physical burden during exercise, 56.3% of swimmer and 31.3% of field and track athletes answered “very hard”, which showed a significant difference (p<0.001) More than 80% of female athletes had experienced a vertigo during exercise (p<0.01). Also, more than 50% of female athletes except badmintoner had experienced an irregular menstruation (p<0.05). Average serum iron level (p<0.05) and TS (p<0.05) of field and track athletes were significantly lower compared to the other athletes. Serum ferritin of badmintoner, field and track athletes and fencer was significantly lower compared to firing and swimmer (p<0.05). Nutrient intakes of female athletes except vitamin B6, niacin and phosphorus were lower than the Korean RDA. Especially, calcium and iron intakes of female athletes were under the 50% of the Korean RDA. Therefore, proper nutrition education and supplementation are required for female athletes to encourage desirable food habits as well as to improve their nutritional status and exercise performance.

• Journal of Nutrition and Health
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• 제26권6호
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• pp.743-757
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• 1993

This study was undertaken to investigate the effects of dietary Fe and Cu levels on Pb accumulation of Pb poisoned rats. 80 male Sprague weaning Dawley rate weighing 80-90g were divided into 8 groups(Pb groups: LFLCPb-low Fe, low Cu and Pb group, LFACPB-low Fe, adequate Cu and Pb group, AFLCPb-adequate Fe, low Cu and Pb group, AFACPb-adequate Fe, adequate Cu and Pb group, without Pb gorups: LFLC-low Fe, low Cu and without Pb grooup, LFAC-low Fe, adequate Cu and without Pb group, AFLC-adequate Fe, low Cu and without Pb group, AFAC-adequate Fe, adequate Cu and without Pb group) according to Pb administration (0, 500ppm in drinking water) and Fe and Cu levels(Fe :6ppm, 40ppm, Cu : 0.5ppm, 0.8ppm) for 12 weeks. The food intake, body weight, gain, and FER of Pb groups were significantly lower than those of without Pb groups(p<0.01, p<0.001, p<0.001). The weights of liver in Pb groups were significantly lower than that of adequate Fe and Cu group in without Pb group(AFAC) (p<0.001). The Cu contents of liver in Pb groups were significantly lower than that of AFAC (p<0.01). The liver Pb accumulation of LFLCPb was significantly higher than those of LFACPb, AFLCPb, AFACPb(p<0.05). The serum Pb content of LFLCPb was significantly higher than those of LFACPb, AFLCPb, AFACPb(p<0.05). The levels of Hb, and Hct, of 12 weeks in Pb poisoned rats were lower than those of 8 weeks, in other words, long term Pb poisoned rats were affected more serious on hematoopoiesis. The fecal excretions of Fe and Cu in Pb groups were significantly higher than those of without Pb groups(p<0.001, p<0.001). The urinary Fe and Cu excretions were significantly higher than those of AFAC(p<0.001, p<0.001). There were significant differences between Pb groups and without Pb groups according to dietary Fe and Cu levels. So, dietary levels of adequate Fe and Cu were effective to reduce Pb accumulation in rats. It were showed that the decrease of food intake, FER, body weight gain, serum level of Fe and Cu in Pb groups than those of without Pb groups. And the increase of liver Pb accumulation, serum Pb levels, fecal and urinary Fe and Cu excretions were showed in Pb groups than those of without Pb groups by long term lead poisoning. Otherwise, adequate Fe and Cu administrations in Pb groups have preventive effects on the Pb poisoned rats.

• Journal of Yeungnam Medical Science
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• 제7권1호
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• pp.95-103
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• 1990

1989년 8월 1일부터 1990년 4월 30일까지 영남대학교 의과대학 산부인과에서 대량출혈이 예상되는 18명의 환자에서 21례의 자가수혈을 시행하여 관찰한 결과 자가수혈의 가장 많은 적응증으로는 전치태반이었으며(10/21) 수술후 자가수혈 및 homologous transfusion의 빈도도 제일 많았다. 출혈위험이 있는 산모 20례중 6례에서 자가수혈로 인해 homologous transfusion을 피할 수 있었다. 혈액 채취전후의 혈압 및 맥박, 호흡 및 reticulocyte등은 통계학적으로 별차이점이 없었고, 채혈전후에 시행하였던 태아감시장치상 약 8례에서 약간의 이상이 나타났으나 대부분 자세변화 및 산소공급으로 20분 이내에 정상으로 돌아왔다. 태어난 신생아중 거대아 1명, 저체중아 3명이었으며 14명이 정상체중아였고, 분만전의 채혈로 인한 이상소견을 발견할 수가 없었다. 채취전후의 혈액학적 변화로소 채취전 Hct는 $34.1{\pm}2.9%$였고, 채취 후는 $31.8{\pm}2.4%$로 유의성이 있었다(P<0.01). 대부분의 혈액 채취시간은 4-8분 정도였으며 채혈시 산모에서 특별한 합병증을 발견하지 못하였다. 3명을 제외하고 15명이 채혈후 3주 이내에 분만하였다. 상기한 여러가지 결과 대량출혈이 예상되는 산모에 있어서 수술전에 혈액을 채취하여 필요할 때 사용할 수 있는 자가수혈은 산모 및 태아에 있어 특별한 이상을 발견할 수 없었다. 그러므로, 산모에 있어서 자가수혈은 homologous transfusion을 피할 수 있는 매우 유용한 한 방법일 수 있다는 결론을 얻었고, 채혈시 나타났던 태아감시상의 여러가지 이상소견에 대해서는 더 많은 연구가 필요할 것으로 사료된다.

• 대한본초학회지
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• 제36권3호
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• pp.15-24
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• 2021

Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

• 대한수혈학회지
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• 제29권3호
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• pp.229-239
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• 2018

순환계는 혈액의 흐름이 원활하게 되도록 그 해부학적 구조와 혈류의 물리학적 특성이 연결되어있다. 혈액의 흐름에 영향을 주는 혈액의 물리학적 특성을 혈유변학적 인자(Hemorheologic factors)라 하며, 혈액의 점도(blood viscosity) 및 적혈구 응집도(erythrocyte aggregation)와 같은 혈유변학적 특성은 헤마토크리트(hematocrit; Hct)와 밀접한 관계를 가진다. 헤마토크리트가 높을수록 혈액 점도가 증가하고 적혈구 응집도는 증가하며 순환을 저해하고 조직에 산소 전달 능력을 방해한다. 혈청 페리틴은 과도하게 있을 경우 산화유리기(oxygen free radial)를 통하여 혈관 내피세포(vascular endothelial cell)와 혈구 세포(blood cell)에 산화 손상을 유발하여 심혈관계 손상을 유발한다. 이러한 기전을 근거로 사혈 혹은 헌혈을 심혈관계 질환의 예방 및 치료에 응용하려는 시도가 오랫동안 있었다. 사혈은 의학에 있어 오랜 역사를 가지면서 특히 한방의 치료적 개념으로 최근까지도 사용되고 있으나 그 과학적 근거가 불충분하고 아직까지 논란의 여지도 있어 근거 중심의 의학을 근간으로 하는 의학에서는 도외시되어왔다. 하지만 혈액량이 증가하면 혈유변학적 인자들(hemorheological factors) 역시 악화되면서 대 순환에서 동맥경화의 발생 및 진행에 영향을 주고, 모세순환(microcirculation)을 악화시키는 것으로 알려져 있으며 심뇌혈관 사건에 영향을 주는 것으로 보고되고 있다. 정기적인 헌혈자(regular blood donors)에게 혈유변학적 인자들을 저명하게 호전시키고 과도한 철분의 함량을 줄여서 산화유리기에 의한 혈구 및 내피세포의 산화 손상을 줄임으로써, 순환계내에서 혈액순환을 촉진시킬 수 있다는 증거들이 보고되고 있다. 헌혈의 효과가 심혈관계 질환에 이익이 된다는 확고한 장기적 코호트 연구결과가 도출된다면 절대적으로 헌혈 혈액량이 부족한 현실과 심혈관계 질환의 예후를 호전시킬 수 있다는 측면에서 중요한 역할을 할 수 있다고 생각된다.

• Nutrition Research and Practice
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• 제13권1호
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• pp.58-63
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• 2019

BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: $CD133^+CD44^+$ cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and $40{\mu}g/mL$ for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA ($ddC_t$). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA ($dC_t$). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner ($5.16{\pm}0.13$ at $0{\mu}g/mL$, $4.79{\pm}0.12$ at $10{\mu}g/mL$, $3.24{\pm}0.08$ at $20{\mu}g/mL$ and $3.99{\pm}0.09$ at $40{\mu}g/mL$; P = 0.0276). Telomerase activities concurrently decreased with telomere length ($1.47{\pm}0.04$, $1.09{\pm}0.01$, $0.76{\pm}0.08$, and $0.88{\pm}0.06$; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.

• BMB Reports
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• 제55권4호
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• pp.198-203
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• 2022

As negative regulators of cytokine signaling pathways, suppressors of cytokine signaling (SOCS) proteins have been reported to possess both pro-tumor and anti-tumor functions. Our recent studies have demonstrated suppressive effects of SOCS1 on epithelial to mesenchymal signaling in colorectal cancer cells in response to fractionated ionizing radiation or oxidative stress. The objective of the present study was to determine the radiosensitizing action of SOCS1 as an anti-tumor mechanism in colorectal cancer cell model. In HCT116 cells exposed to ionizing radiation, SOCS1 over-expression shifted cell cycle arrest from G2/M to G1 and promoted radiation-induced apoptosis in a p53-dependent manner with down-regulation of cyclin B and up-regulation of p21. On the other hand, SOCS1 knock-down resulted in a reduced apoptosis with a decrease in G1 arrest. The regulatory action of SOCS1 on the radiation response was mediated by inhibition of radiation-induced Jak3/STAT3 and Erk activities, thereby blocking G1 to S transition. Radiation-induced early ROS signal was responsible for the activation of Jak3/Erk/STAT3 that led to cell survival response. Our data collectively indicate that SOCS1 can promote radiosensitivity of colorectal cancer cells by counteracting ROS-mediated survival signal, thereby blocking cell cycle progression from G1 to S. The resulting increase in G1 arrest with p53 activation then contributes to the promotion of apoptotic response upon radiation. Thus, induction of SOCS1 expression may increase therapeutic efficacy of radiation in tumors with low SOCS1 levels.

• 한국동물위생학회지
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• 제45권1호
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• pp.47-54
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• 2022

Subcutaneous abscesses, which occur mainly in goats and sheep, are lymph node abscesses caused by Corynebacterium pseudotuberculosis infection, and are divided into internal, external, and mixed types depending on the type of occurrence. While diagnostic methods for subcutaneous abscesses have been continuously studied, research reports for effective treatment and management of subcutaneous abscesses are inadequate. Therefore, this study was conducted to determine the changes in biometric information related to the inflammatory markers of goats induced by subcutaneous abscesses by infection with C. pseudotuberculosis. For this, hematological tests, analysis of inflammatory indicators, and analysis of serum proteins through electrophoresis separation of goats with healthy goats and goats inoculated with C. pseudotuberculosis to induce subcutaneous abscesses were compared and analyzed by date, and the differences and characteristics were identified periodically. As a result, in goats induced with subcutaneous abscesses, anemia findings related to a rapid decrease in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) were observed, and a significant increase in inflammatory cells expressed in total white blood cell (WBC), neutrophil, and monocytes was observed. And the levels of acute phase protein (APP) such as fibrinogen, haptoglobin, and serum amyloid A (SAA) were observed to increase rapidly immediately after infection. In addition, in the results of electrophoretic analysis of serum proteins, it was observed that the levels of α-globulin and β-globulin were significantly increased in goats with subcutaneous abscesses. That is, when looking at these changes, it was found that the systemic inflammatory response of goats was rapidly induced immediately after infection with the C. pseudotuberculosis pathogen. Through this study, it was possible to identify changes in the biomarkers of goats with subcutaneous abscesses, which had not been reported. Furthermore, these analyzed data are thoughts to be of great help in identifying, treating, and managing the goats of subcutaneous abscesses.

• 동의신경정신과학회지
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• 제35권2호
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• pp.141-151
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• 2024

Objectives: To compare symptoms and characteristics among patients diagnosed with Hwabyung according to their yin-yang personality types. Methods: A structured clinical interview was conducted to diagnose Hwabyung, followed by a cross-sectional study involving a total of 118 Hwabyung patients (95 females). Various measures including the Sasang Personality Questionnaire (SPQ), Hwabyung Comprehensive Test (HCT), HwaByung Scale (HBS), State-Trait Anger Expression Inventory (STAXI), Beck Depression Inventory-II (BDI-II), Beck Anxiety Inventory (BAI), Eogul Scale (ES), and Patient Health Questionnaire-15 (PHQ-15) were administered. Results: SPQ-total showed significant negative correlations with HBS-personality and STAXI-control, and a significant positive correlation with STAXI-out. SPQ-behavior showed significant positive correlations with HBS-symptom, STAXI-trait, STAXI-in, STAXI-out, BDI-II, BAI, ES, and PHQ-15. SPQ-cognition exhibited significant negative correlations with HBS-personality, STAXI-control, and ES. SPQ-emotion had significant negative correlations with HBS-personality and STAXI-in and a significant positive correlation with STAXI-out. SPQ-Yin demonstrated a significantly higher HBS-personality than SPQ-Yang. SPQ-Yang exhibited significantly higher STAXI-out than SPQ-Yin, whereas SPQ-Yin showed significantly higher STAXI-control and STAXI-in than SPQ-Yang. Although differences in depression, anxiety, resentment, and physical symptoms between yin and yang were not statistically significant, variations in interpretation criteria were observed. Conclusions: Anger expression patterns and physical/psychological symptoms among Hwabyung patients are different based on their yin-yang personality types. These findings can inform the prediction of symptoms in Hwabyung patients according to their yin-yang personality types and aid in establishing appropriate treatment plans.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1720-1725
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• 2011

본 연구에서는 아스파르트산, 탄산칼슘 및 황산철을 반응시켜 아스파르트산 킬레이트 철분(aspartic acid chelated iron, Asp-Fe)을 제조하고 Asp-Fe의 철분결핍 쥐(iron-deficient rat, ID)에서의 생물학적 유용성을 확인하였다. 시험군은 철분이 함유된 식이를 섭취한 정상군(NC), 철분 결핍식이를 1개월간 투여하여 철분 결핍 상태를 유도한 쥐(ID)에 생리식염수를 공급한 결핍 대조군(ID+C), 철분 결핍 쥐에 햄철(heme-Fe) 투여군(ID+heme-Fe) 및 Asp-Fe 투여군(ID+Asp-Fe)으로 나누어 실시하였다. 그 결과 식이섭취량, 장기무게, 체중증가 정도에서 각 군에 따른 차이가 없는 것으로 나타났다. 7일간 투여 후 혈액 중 철분의 함량을 측정한 결과 결핍쥐에 Asp-Fe 투여군(175.2 ${\mu}g$/dL)과 heme-Fe 투여군(140.8 ${\mu}g$/dL)은 결핍 대조군(96.1 ${\mu}g$/dL)보다 유의적인 수준으로 증가하였다. 총 철분 결합능(total iron binding capacity, TIBC)를 측정한 결과 Asp-Fe 투여군(735.4 ${\mu}g$/dL)은 결핍 대조군(841.9 ${\mu}g$/dL)보다 유의적 수준으로 정상화되었다. 헤마토크리트(HCT) 수치를 측정한 결과에서 Asp-Fe 및 heme-Fe 모두 결핍 대조군보다 증가하는 경향은 보였지만 유의적인 차이는 없었다. 흡수율에서는 heme-Fe의 경우 21.3%인 반면에 Asp-Fe의 경우 50.2%로 약 2.3배 높은 것으로 나타났으며, 혈청에서의 철분농도 및 transferrin saturation(TS)는 heme-Fe 투여군 및 결핍 대조군에 비하여 Asp-Fe 투여군이 유의하게 높은 수준을 유지하였다. 이상의 결과로 미루어 볼 때 아미노산 킬레이트 철분은 heme-Fe과 유사한 수준의 생체 이용율을 가지고 있으며, 철분 결핍을 회복시키는데 매우 효과적인 보충제로서 이용될 수 있을 것으로 사료된다.