• Toxicological Research
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• 제8권2호
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• pp.255-263
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• 1992

SKI 2053R and SKI 2053R-human serum albumin(HSA) mixture were examined for their antigenicity in Hartley guinea pigs as well as C57BL/6 mice in comparison with distilled water (DW), HSA and DW-HSA conjugate. Several antigenicity tests, including acitive systemic anaphylaxis(ASA), passive systemic anaphylaxis (PSA), passive cutaneous anaphylaxis (PCA) and indirect hamagglutination test (IHA), were performed according to the Established Regulations of National Institute of Safety Research. The results were as follows: 1. When guinea pigs were sensitized with SKI2053R or SKI2053R-HSA emulsified with complete Freund's adjuvant(CFA), these animals showed negative reactions in ASA and PSA. 2.No blue spot was observed on the back skin of guinea pigs in the PCA test. 3. Sera from guinea pigs revealed a negative reaction in IHA. 4.Guinea pigs were sensitized by HSA emulsified with CFA as a positive control, and these animals showed positive reactions in ASA, PSA, PCA, and IHA. As shown above, SKI2053R was considered to possess neither antigenic, nor haptenic properties, and confirmed not to have the haemagglutinating activity.

• Clinics in Shoulder and Elbow
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• 제21권4호
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• pp.186-191
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• 2018

Background: To determine the normal range of humeral head positioning on magnetic resonance imaging (MRI). Methods: We selected normal subjects (64 patients; group A) to study the normal range of humeral head positioning on the glenoid by MRI measurements. To compare the MRI measurement method with the computed tomography (CT), we selected group B (70 patients) who underwent both MRI and CT. We measured the humeral-scapular alignment (HSA) and the humeral-glenoid alignment (HGA). Results: The HSA in the control group was $1.47{\pm}1.05mm$, and the HGA with and without reconstruction were $1.15{\pm}0.65mm$ and $1.03{\pm}0.59mm$, respectively, on MRI. In the test group, HSA was $2.67{\pm}1.47mm$ and HGA with and without reconstruction was $1.58{\pm}1.16mm$ and $1.49{\pm}1.08mm$, on MRI. On CT, the HSA was $1.72{\pm}1.01mm$, and HGA with and without reconstruction were $1.54{\pm}0.96mm$ and $1.59{\pm}0.93mm$, respectively. HSA was significantly different according to image modality (p=0.0006), but HGA was not significantly different regardless of reconstruction (p=0.8836 and 0.9234). Conclusions: Although additional CT scans can be taken to measure decentering in patients with rotator cuff tears, reliable measurements can be obtained with MRI alone. When using MRI, it is better to use HGA, which is a more reliable measurement value based on the comparison with CT measurement (study design: Study of Diagnostic Test; Level of evidence II).

• Genomics & Informatics
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• 제19권3호
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• pp.29.1-29.10
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• 2021

In our previous studies, we have demonstrated the association of certain variants of the thyroid-stimulating hormone receptor (TSHR), thyroid peroxidase (TPO), and thyroglobulin (TG) genes with congenital hypothyroidism. Herein, we explored the mechanistic basis for this association using different in silico tools. The mRNA 3'-untranslated region (3'-UTR) plays key roles in gene expression at the post-transcriptional level. In TSHR variants (rs2268477, rs7144481, and rs17630128), the binding affinity of microRNAs (miRs) (hsa-miR-154-5p, hsa-miR-376a-2-5p, hsa-miR-3935, hsa-miR-4280, and hsa-miR-6858-3p) to the 3'-UTR is disrupted, affecting post-transcriptional gene regulation. TPO and TG are the two key proteins necessary for the biosynthesis of thyroid hormones in the presence of iodide and H₂O₂. Reduced stability of these proteins leads to aberrant biosynthesis of thyroid hormones. Compared to the wild-type TPO protein, the p.S398T variant was found to exhibit less stability and significant rearrangements of intra-atomic bonds affecting the stoichiometry and substrate binding (binding energies, ΔG of wild-type vs. mutant: -15 vs. -13.8 kcal/mol; and dissociation constant, K_d of wild-type vs. mutant: 7.2E^-12 vs. 7.0E^-11 M). The missense mutations p.G653D and p.R1999W on the TG protein showed altered ΔG(0.24 kcal/mol and 0.79 kcal/mol, respectively). In conclusion, an in silico analysis of TSHR genetic variants in the 3'-UTR showed that they alter the binding affinities of different miRs. The TPO protein structure and mutant protein complex (p.S398T) are less stable, with potentially deleterious effects. A structural and energy analysis showed that TG mutations (p.G653D and p.R1999W) reduce the stability of the TG protein and affect its structure-functional relationship.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1216-1221
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• 2006

A protein array was fabricated for a miniaturized immunoassay using microcontact printing ($\mu$CP). A polydimethylsiloxane (PDMS) stamp with a 5 $\mu$m$\times$5 /$\mu$m dimension was molded from a silicon master developed by photolithography. Under optimal fabrication conditions, including the baking, incubation, and exposure time, a silicon master was successfully fabricated with a definite aspect ratio. An antibody fragment was utilized as the ink for the $\mu$CP, and transferred to an Au substrate because of the Au-thiol (-SH) interaction. The immobilization and antibody-antigen interaction were investigated with fluorescence microscopy. When human serum albumin (HSA) was applied to the protein array fabricated with an antibody against HSA, the detection limit was 100 pg/ml of HSA when using a secondary antibody labeled with a fluorescence tag. The fabricated protein array maintained its activity for 14 days.

• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.33-40
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• 2014

Polysaccharides have attracted great attention for their wide range of applications in biological and medical fields. In this paper, the interaction of polysaccharides with human serum albumin (HSA) was systematically investigated by fluorescence (FL) spectroscopy and circular dichroism (CD) spectra under different conditions. The Stern-Volmer quenching constants ($K_a$) at different ionic strength and pH were calculated, and information of the structural features of HSA was discussed. FL and CD results indicate that both hydrophobic and electrostatic interactions play important roles during the binding process. The quenching of the fluorescence resulting the binding of polysaccharides and HSA is static.

• 산업공학
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• 제12권1호
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• pp.94-101
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• 1999

The set covering(SC) problem is the problem of covering all the rows of an $m{\times}n$ matrix of ones and zeros by a subset of columns with a minimal cost. It has many practical applications of modeling of real world problems. The SC problem has been proven to be NP-complete and many algorithms have been presented to solve the SC problem. In this paper we present hybrid simulated annealing(HSA) algorithm based on the Simulated Annealing(SA) for the SC problem. The HSA is an algorithm which combines SA with a crossover operation in a genetic algorithm and a local search method. Our experimental results show that the HSA obtains better results than SA does.

• KSBB Journal
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• 제13권2호
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• pp.125-132
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• 1998

Protein affinity membrane was prepared via the coating of chitosan gel on the porous flat polysulfone membrane surface, followed by the immobilization f the reactive dye (Cibacron Blue 3GA) to the chitonsan gel. The maximum protein binding capacity of affinity membrane was about 70${\mu}g/cm^2$ determined by the batch adsorption experiments of human serum albumin (HSA). Using module of this membrane, the characteristics of protein chromatography were investigated through the experiments of elution and frontal chromatography of HSA. This membrane module promises as a chromatography column, since it represented a lower pressure drop and a greater reproducibility. The protein separation ratio was significantly influenced by the flow rate of mobile phase and the injection quantity of HSA. The dynamic protein binding capacity of module decreased from the equilibrium binding capacity with increasing flow rate and approached the value of 15 - 20 ${\mu}g/cm^2$ for flow rates above 6 mL/min.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2022년도 추계학술발표대회
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• pp.60-62
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• 2022

SYCL은 OpenCL 디바이스를 위해 추상화한 C++ 프로그래밍 모델이다. OpenCL에 비해 SYCL은 높은 생산성 등 C++이 가지고 있는 강점을 보유하며, 인텔이 이기종 컴퓨팅을 위한 개발 언어로 SYCL 기반의 DPC++을 출시함에 따라 많은 주목을 받고 있다. 우리는 여러 SYCL 구현물들 중에서 NVIDIA, AMD 등 다양한 GPU를 지원하고, 코드의 수정 및 추가가 용이한 hipSYCL를 채택하여 여러 연구를 진행하고 있다. 본 논문에서는 hipSYCL 구조 내에 AMD GPU를 위한 HIP 백엔드 플러그인을 대체할 수 있는 새로운 백엔드 플러그인을 제안한다. 이 플러그인은 HSA 런타임 API를 사용하여 기존의 플러그인보다 계층 구조를 줄이고 경량화하였다.

• 한국강구조학회 논문집
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• 제25권4호
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• pp.431-440
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• 2013

본 연구에서는 HSA800 후판 강재에 적합한 용접재를 선정하고자 표준인장실험을 수행하였다. 본 연구 수행 당시 HSA800 강재에 적용가능한 용접재로 GMAW 용접재(외국산)와 FCAW 용접재(국내산) 두 가지가 추천되어 이들을 사용하였다. 맞댐용접상세와 루트간격을 주요 실험변수로하여 표준인장실험을 통해 용접부의 성능을 평가하였다. 강도에 대한 설계요구조건은 두 용접재 모두 만족하였으나 연성능력 측면에서 GMAW 용접재 보다는 FCAW 용접재가 일관되고 우수한 거동을 보임이 실험적으로 확인되었다. 특히 GMAW 용접재는 용접효율과 작업성의 문제로 현장에서의 상향용접이 어려운 것으로 파악되었다. 같은 완전용입용접이라도 Single bevel보다는 V-groove가 안정적인 구조거동을 보이는 것으로 나타났다. 또한 현행 용접기준의 표준루트 간격을 벗어날 경우 용착의 어려움으로 비정상 파단면이 형성되고 접합성능도 저하됨이 확인되었다. 부분용입용접 접합부의 실험결과에 의할 때 현행 AISC 기준의 부분용입용접부 강도규정은 매우 보수적임이 확인되었다.

• KSBB Journal
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• 제24권1호
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• pp.41-46
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• 2009

식물성 오일은 다양한 생물제품 생산에 적합한 저가의 원료이다. 식물성 오일의 주성분인 oleic acid와 linoleic acid와 같은 불포화 지방산의 함량은 콩기름의 경우 각각 22%와 55%, 옥수수기름의 경우 26%와 60%, 유채기름의 경우 61%와 21%에 달한다. Hydroxy나 keto group으로 치환된 불포화 지방산들은 다른 지방산에 비해 높은 점도와 반응성 등의 특별한 화학적 특성 때문에 가소제, 계면활성제, 윤활유, 세제 등에 사용되는 유용한 산업 화합물이다. 본 연구에서는 Flavobacterium sp. strain DS5 (NRRL B-14859)를 oleic acid로부터 중간생성물로서 10-hydroxystearic acid (10-HSA)를 거쳐 10-ketostearic acid (10-KSA)로의 전환에 이용하였다. 두 개의 생물전환 생성물인 10-KSA와 10-HSA는 GC, GC-MS, $^1H-NMR$, $^{13}C-NMR$을 이용하여 정량 및 정성분석을 수행하였다. 플라스크 배양에서 10-KSA와 10-HSA의 최대 생산량은 각각 3.4 g/L와 0.5 g/L 이었다. 10-KSA 생산을 위한 최적 glucose 농도 yeast extract 농도, oleic acid 첨가시간, 첨가부피는 각각 20 g/L 이하, 5 g/L 이상, 접종 후 18 h, 0.3 ml/50 ml 이었다.