Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antigenicity test of cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II)(SKI 2053R) in Guinea Pigs and Mice

기니픽 및 마우스에서 cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II)(SKI 2053R)의 항원성시험

  • Lee, Yong-Soon (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (College of Veterinary Medicine, Seoul National University) ;
  • Shin, Dong-Jin (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Hyoung-Ook (College of Veterinary Medicine, Seoul National University) ;
  • Cho, Jae-Jin (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Bae-Hwan (College of Veterinary Medicine, Seoul National University) ;
  • Nam, Ki-Hoan (College of Veterinary Medicine, Seoul National University) ;
  • Seo, Kwang-Won (College of Veterinary Medicine, Seoul National University)
  • 이영순 (서울대학교 수의과대학 공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실) ;
  • 신동진 (서울대학교 수의과대학 공중보건학교실) ;
  • 김형욱 (서울대학교 수의과대학 공중보건학교실) ;
  • 조재진 (서울대학교 수의과대학 공중보건학교실) ;
  • 김배환 (서울대학교 수의과대학 공중보건학교실) ;
  • 남기환 (서울대학교 수의과대학 공중보건학교실) ;
  • 서광원 (서울대학교 수의과대학 공중보건학교실)
  • Published : 1992.12.01
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Abstract

SKI 2053R and SKI 2053R-human serum albumin(HSA) mixture were examined for their antigenicity in Hartley guinea pigs as well as C57BL/6 mice in comparison with distilled water (DW), HSA and DW-HSA conjugate. Several antigenicity tests, including acitive systemic anaphylaxis(ASA), passive systemic anaphylaxis (PSA), passive cutaneous anaphylaxis (PCA) and indirect hamagglutination test (IHA), were performed according to the Established Regulations of National Institute of Safety Research. The results were as follows: 1. When guinea pigs were sensitized with SKI2053R or SKI2053R-HSA emulsified with complete Freund's adjuvant(CFA), these animals showed negative reactions in ASA and PSA. 2.No blue spot was observed on the back skin of guinea pigs in the PCA test. 3. Sera from guinea pigs revealed a negative reaction in IHA. 4.Guinea pigs were sensitized by HSA emulsified with CFA as a positive control, and these animals showed positive reactions in ASA, PSA, PCA, and IHA. As shown above, SKI2053R was considered to possess neither antigenic, nor haptenic properties, and confirmed not to have the haemagglutinating activity.

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