KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics of Protein Chromatography by Affinity Membrane Mudule

친화성 막모듈에 의한 단백질 크로마토그래픽 특성

  • 이광진 (충북대학교 공과대학 화학공학부) ;
  • 염경호 (충북대학교 공과대학 화학공학부)
  • Published : 1998.04.01
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Abstract

Protein affinity membrane was prepared via the coating of chitosan gel on the porous flat polysulfone membrane surface, followed by the immobilization f the reactive dye (Cibacron Blue 3GA) to the chitonsan gel. The maximum protein binding capacity of affinity membrane was about 70${\mu}g/cm^2$ determined by the batch adsorption experiments of human serum albumin (HSA). Using module of this membrane, the characteristics of protein chromatography were investigated through the experiments of elution and frontal chromatography of HSA. This membrane module promises as a chromatography column, since it represented a lower pressure drop and a greater reproducibility. The protein separation ratio was significantly influenced by the flow rate of mobile phase and the injection quantity of HSA. The dynamic protein binding capacity of module decreased from the equilibrium binding capacity with increasing flow rate and approached the value of 15 - 20 ${\mu}g/cm^2$ for flow rates above 6 mL/min.

Keywords

References

  1. Bioseparations: Downstream Processing for Biotechnology Belter,P.A.;E.L.Cussler;W.S.Hu
  2. Recovery Processes for Biological Materials Kennedy,J.F.;J.M.S.Cabral
  3. Affinity Chromatography: A Practical Approach Dean,P.D.G.;W.S.Johnson;F.A.Middle
  4. Affinity Chromatography Lowe,C.R.;P.D.G.Dean
  5. Ind. Eng. Chem. Fundam. v.25 no.4 Scaling Up of Gradient Elution Chromatography Gibbs,S.J.;E.N.Lightfoot
  6. Biotech. & Bioeng. Symp. no.14 Bioseparations: Column Design Factors in Liquid Chromatography Ladisch,M.R.;M.Voloch;B.Jacobson
  7. Chem. Eng. Sci. v.47 Fast Protein Chromatography on Analytical and Preparative Scale Using Modified Microporous Membranes Briefs,K.G.;M.R.Kula
  8. J. Memb. Sci. v.117 Supported Chitosan-Dye Affinity Membranes and their Protein Adsorption Zeng,X.;E.Ruckenstein
  9. Master Thesis, Dept. of Industrial Chem. Eng., Chungbuk Nat. Univ. Separation Characteristics of Protein by Affinity Chromatography Using Membrane Modules Lee,K.J.
  10. Bio/Technology v.6 Membrane-Based Affinity Technology for Commercial Scale Purifications Brandt,S.;R.A.Goffe;S.B.Kessler;J.L.O'Connor;S.E.Zale
  11. Modification of Microfiltration Membrane as Dye-Ligand Adsorbents for the Isolation of Enzymes from Crude Extracts, Separations for Biotechnology Champluvier,B.;M.R.Kula;D.L.Pyle(ed.)
  12. J. Chromatogr. v.539 Microfiltration Membranes as Pseudo-Affinity Adsorbents: Modification and Comparison with Gel Beads Champluvier,B.;M.R.Kula
  13. J. Memb. Sci. v.117 Comparison of Protein Adsorption by Anion-Exchange Interaction onto Porous Hollow-Fiber Membrane and Gel Bead-Packed Bed Kubota,N.;S.Miura;K.Saito;K.Sugita;K.Watanabe;T.Sugo
  14. Protein Purification(2nd ed.) Scopes,R.K.
  15. Biochem. Soc. Trans. v.9 Triazine-Dye Affinity Chromatography Atkinson,T.;P.M.Hammond;R.D.Hartwell;P.Hughes;M.D.Scawen;R.F.Sherwood;D.A.P.Small;C.J.Bruton;M.J.Harvey;C.R.Lowe
  16. 염료화학 남성석;서보영;이대수
  17. Biochem. J. v.167 The Effect of Matrix on the Binding of Albumin to Immobilized Cibacron Blue Angel,S.;P.D.G.Dean
  18. Anal. Biochem. v.83 Determination of Cibacron Blue F3GA Substitution in Blue Sephadex and Blue Dextran-Sepharose Chambers,G.K.
  19. Dye-Grafted, Poly(ethylene imine)-Coated, Formed-in-place Class Affinity Membranes for Selective Separation of Proteins, Polymers of Biological and Biomedical Significance Li,Y.;H.G.Spencer;W.Shalaby(et al.)(ed.)
  20. Biotech. & Bioeng. v.40 Dye-Ligand Membranes as Selective Adsorbents for Rapid Purification of Enzymes: A Case Study Champluvier,B.;M.R.Kula
  21. AIChE J. v.35 no.5 Hollow-Fiber Liquid Chromatography Ding,H.;M.C.Yang;D.Schisla;E.L.Cussler