• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2002.11a
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• pp.319.1-319
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• 2002

A frequency-domain PES estimation and its prediction method are proposed for the tightly-coupled servo/mechanical design of high-TPI HDD system above 100kTPI. The major two disturbance energies which are related with mechanical vibrations inside of HDD are used to predict the drive-level PES, while considering closed-loop servo dynamics. One is the torque disturbance which mainly comes from aerodynamic excitation of HSA system and the other is the displacement disturbance from disk-spindle dynamics. (omitted)

• Coupled systems mechanics
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• v.13 no.1
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• pp.1-19
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• 2024

The effectiveness of coupling with a bolted rim is assessed in this research using a newly designed optimization algorithm. The current study, which is provided here, evaluates 10 contemporary metaheuristic approaches for enhancing the coupling with bolted rim design problem. The algorithms used are particle swarm optimization (PSO), crow search algorithm (CSA), enhanced honeybee mating optimization (EHBMO), Harmony search algorithm (HSA), Krill heard algorithm (KHA), Pattern search algorithm (PSA), Charged system search algorithm (CSSA), Salp swarm algorithm (SSA), Big bang big crunch optimization (B-BBBCO), Gradient based Algorithm (GBA). The contribution of the paper isto optimize the coupling with bolted rim problem by comparing these 10 algorithms and to find which algorithm gives the best optimized result. These algorithm's performance is evaluated statistically and subjectively.

• Bulletin of the Korean Chemical Society
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• v.28 no.10
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• pp.1746-1750
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• 2007

The second-order scattering (SOS) phenomenon of the interaction of Pd nanoparticles with protein was reported and a simple, sensitive, palladium nanoparticle-based assay for trace amount of protein with SOS technique was developed. The SOS intensities were significantly enhanced due to the interaction of Pd nanoparticles with bovine serum albumin (BSA) or human serum albumin (HSA) at pH 3.5 or 4.0, respectively. The maximum SOS peak appeared at 260/520 nm (λex/λem). The optimal experiment conditions, affecting factors and the influence of some coexisting substances were checked. The SOS intensity increased proportionally with the increase of Pd concentration below 3.0 × 10?5 mol·L?1, while declined gradually above 4.0 × 10?5 mol·L?1. BSA within the range of 0.01-2.6 μg·mL?1 and HSA of 0.01-1.7 μg·mL?1 can be detected with this method and the detection limits were 2.3 and 11.2 ng·mL?1, respectively. The method was successfully applied to the quantitative detection of total protein content in human serum samples with the maximum relative standard deviation (RSD) lower than 2.6% and the recoveries over the range of 99.5-100.5%.

• Journal of Pharmaceutical Investigation
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• v.40 no.3
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• pp.175-180
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• 2010

Albumin-modification has been viewed as one of the most effective ways of extending the short in vivo lifetimes of peptide drugs by delaying glomerular filtration. In this study, we describe a new type 2 anti-diabetic exendin-4 (Ex4) peptide derivative with significant binding ability to human serum albumin (HSA). This exendin-4 derivative consists of a 4-branched polyethylene glycol $(PEG)_{5k}$ (Mw: 20 kDa) modified with three stearylamines ($C_{18}-NH_2$) and one exendin-4 on its branches. PEG and stearylamine were selected to provide functionality to increase molecular size and bind to albumin, respectively. This derivative ($3C_{18}-4PEG_{5k}$-Ex4) was shown to have larger molecular size (Ca. 152 kDa) than actual (25.0 kDa) when subjected to size-exclusion chromatography, and the fluorescein-tagged $3C_{18}-4PEG_{5k}$-Ex4 displayed significant binding to the HSA-immobilized Sepharose CL-4B resin using confocal laser scanning microscopy. Furthermore, $3C_{18}-4PEG_{5k}$-Ex4 was found to have acceptable anti-hyperglycemic efficacy via three consecutive oral glucose tolerance testings (OGTT) in fasted type 2 diabetic db/db mice. The $HD_{total}$ value ($57.6{\pm}12.3%$) of $3C_{18}-4PEG_{5k}$-Ex4 at a 50 nmol/kg dose was 2-fold greater than that ($31.0{\pm}8.7%$) of native exendin-4 in non-fasted db/db mice. Especially, the blood glucose levels in the mice group treated with $3C_{18}-4PEG_{5k}$-Ex4 did not rebound to ~150 mg/dL until 24 h after the injection, which obviously shows the extended hypoglycemia. We believe that this derivative has great pharmaceutical potential as a novel long-acting type 2 anti-diabetic injection treatment.

• Applied Chemistry for Engineering
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• v.31 no.4
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• pp.416-422
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• 2020

In this study, eco-friendly biodegradable materials were prepared using poly(lactic acid) (PLA), poly(butylene adipate-co-terephthalate) (PBAT), and hydrogenated castor oil power (HCO) as an additive. The prepared PLA/PBAT/HCO blended films were characterized by the scanning electron microscope (SEM) and fourier-transform infrared spectroscopy (FT-IR). The results of SEM analysis indicated that PLA/PBAT (8 : 2) blended films added HCO showed no rough area, crack, or large agglomeration when compared with those adding various additives (12-hydroxy stearic acid (12HSA) and cellulose). The FT-IR results indicated the presence of specific peak of HCO in the PLA/PBAT blended films, and its peak intensity increased with increasing HCO content (0~5.0 wt%). Tensile strength, elongation at break, and water barrier and thermal properties of the prepared PLA/PBAT/HCO blended films were also investigated, indicating that the physical and thermal properties was improved more than three times by the addition of HCO. The biodegradability test in soil revealed that the prepared biodegradable materials were degraded by about 6.0~20% after 90 days.

• Journal of Korean Society of Steel Construction
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• v.26 no.4
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• pp.361-373
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• 2014

As the height and beam span of buildings built in the construction market increase, increasingly higher quality is being required of the construction materials. In response to this trend, 800MPa tensile strength class steel was developed in domestic company. Currently, experiments applying flexural member, compression member, and connections are continuously conducted, but a design guideline for high strength steel has yet to be established. Among those construction materials, for the high strength steel beam-to-column connections, the evaluation of implementing ductile connections for the high strength steel beam-to-column connections is producing pessimistic results and the number of related researches is inadequate because of the high yield ratio, which is the characteristic of high strength steel. This study on implementation of ductile connections made of high strength steel was conducted using the connection detail as the variable, for the purpose of enhancing the deformation capacity of high strength steel beam-to-column connections. Cyclic loading test and nonlinear finite element analysis were conducted with full-scale mock-up connection models with the applied connection details. As a result, the structural performance of high-strength steel beam-to-column connection with presented detail was contented with demand of Special Moment Frames of KBC standard.

• Korean Journal of Food Science and Technology
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• v.41 no.4
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• pp.405-412
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• 2009

The purpose of this study was to determine the inhibitory effect of a glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein, 75 kDa) on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3 cells. This experiment evaluated the production of intracellular reactive oxygen species (ROS), the activities of mitogenactivated protein kinase (MAPK), transcription factor (c-jun), and cyclooxygenase (COX)-2, and histamine release in cells. The results showed that the CTB glycoprotein inhibited histamine release and COX-2 expression induced by IgE in the cells. The CTB glycoprotein also had suppressive effects on the expressions of ERK1/2, p38 MAPK, c-jun, and the production of intracellular ROS in IgE-treated RBL-2H3 cells. The activities of c-jun and COX-2 were collectively blocked by ERK1/2 inhibitor (PD98059) and p38 MAPK inhibitor (SKF86002), respectively. Hence, we speculate that CTB glycoprotein might be a component with potential use in the preparation of health supplements for the prevention of allergic diseases.

• Molecules and Cells
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• v.39 no.7
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• pp.543-549
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• 2016

MicroRNAs (miRNAs) have been reported to be involved in many neurodegenerative diseases. The present study focused on the role of hsa-miR-144-3p in one of the neuro-degenerative diseases, Parkinson's disease (PD). Our study showed a remarkable down-regulation of miR-144-3p expression in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated SH-SY5Y cells. MiR-144-3p was then overexpressed and silenced in human SH-SY5Y cells by miRNA-mimics and miRNA-inhibitor transfections, respectively. Furthermore, ${\beta}$-amyloid precursor protein (APP) was identified as a target gene of miR-144-3p via a luciferase reporter assay. We found that miR-144-3p overexpression significantly inhibited the protein expression of APP. Since mitochondrial dysfunction has been shown to be one of the major pathological events in PD, we also focused on the role of miR-144-3p and APP in regulating mitochondrial functions. Our study demonstrated that up-regulation of miR-144-3p increased expression of the key genes involved in maintaining mitochondrial function, including peroxisome proliferator-activated receptor ${\gamma}$ coactivator-$1{\alpha}$(PGC-$1{\alpha}$), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM). Moreover, there was also a significant increase in cellular ATP, cell viability and the relative copy number of mtDNA in the presence of miR-144-3p overexpression. In contrast, miR-144-3p silencing showed opposite effects. We also found that APP overexpression significantly decreased ATP level, cell viability, the relative copy number of mtDNA and the expression of these three genes, which reversed the effects of miR-144-3p overexpression. Taken together, these results show that miR-144-3p plays an important role in maintaining mitochondrial function, and its target gene APP is also involved in this process.

• Analytical Science and Technology
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• v.31 no.6
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• pp.225-231
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• 2018

Benzoic acid, methyl paraben, and butyl paraben are preservatives that have been used in pharmaceutical, cosmetic, and food products. However, as their toxicities for human have been reported, many nations and organizations including Korea have established a regulation limit for thier usage of these preservatives in food products. The present study developed the isotope dilution liquid chromatography mass spectrometry method for accurate determination of three target preseratives in soysauce. In this study, the isotope dilution liquid chromatography mass spectrometry method was developed for accurate determination of three target preservatives in soy sauce. LC separation was optimized considering the pKa of benzoic acid which is lower than those of methyl and butyl parabens. A C18 column was used with 5 mM ammonium acetate and methanol as mobile phases. Mass spectrometry was operated in negative mode and selected reaction monitoring mode (SRM). Soy sauce sample was cleaned-up with C18 SPE cartridge for removing matrix inferences and color material. Optimized conditions and the method were validated with soy sauce reference materials for the analysis of food preservatives from Health Science Authority in Singapore. The measured values of benzoic acid, methyl and butyl paraben agreed well with reference values within their uncertainties.

• Journal of Microbiology and Biotechnology
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• v.18 no.10
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• pp.1695-1700
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• 2008

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16-mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of $Zn(NO_3)_2\cdot 6H_2O$. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate-buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.