• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.732-738
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• 2001

This study was attempted to isolate and identify the anticancer compounds from Eucommia ulmoides leaves using a human colon cancer cell line HCT-116. The petroleum ether extracts with anticancer activity was chromatographed on silica gel TLC and finally anticancer compounds was purified by HPLC. Their chemical structures were roughly elucidate by UV-VIS absorption spectral data HPLC elution pattern and FAM/MS spectroscopy. From this study these compounds were suspected to be pheophytin a formed by the removal of $Mg^{2+}$ from chlorophyll a and pyropheophytina formed by the removal of acetate group from pheophytin a respectively. To confirm the anticancer effects against HCT-116 cancer cell petroleum ether extract fractions of column chromatography and fractions separated on TLC were tested. All samples tested including the extract of petroleum ether fractions of column chromatograph and three bands (0.13,0.19,0.25) of TLC appeared to inhibit the growth of HCT-116 cancer cell however especially 0.19 and 0.25 fractions separated on TLC plate revealed the strongest effect. These results suggest that chlorophyll derivatives in Eucommia ulmoides may be potential anticancer agents against a human colon cancer cell HCT-116.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.546-551
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• 2002

Nutritional composition and phenolic compounds of raw propolis collected from falseacacia (Robinia pseudoacacia L.) and chestnut tree (Castanea crenata), and their 70% ethanol extracts of propolis (EEP) were analyzed. Propolis had high crude lipid content, but no significant differences in general compositions in terms of collection area and plant origins. Mineral contents varied greatly depending on the plant origins, with falseacacia propolis showing the highest mineral content. Sixteen amino acids were analyzed, among which aspartic acid content was the highest at $328.4{\sim}410.6\;mg%$ and methionine the lowest at $0{\sim}21.1\;mg%$. Extraction yield for EEP was relatively high at $64.2{\sim}81.9%$, and total polyphenol and flavonoid contents were $13.9{\sim}23.7$ and $8.6{\sim}10.8%$, respectively. HPTLC and HPLC analysis on the phenolic compounds revealed the overall chromatographic patterns were almost equal, showing similar polyphenol compositions between the propolis. About 16 peaks were identified by HPLC analysis, among which 6 peaks of p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid, and chrysin were identified.

• Korean Journal of Pharmacognosy
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• v.39 no.3
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• pp.179-185
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• 2008

Three species, such as Rheum palmatum L., R. tanguticum Maxim. and R. officinale Baillon are recognized as the source plants of Rhei Rhizoma in Korean Pharmacopeia. However, other herbal sources such as R. undulatum L. and Rumex crispus L. have been often misused as Rhei Rhizoma. A pattern analysis method to discriminate Rhei Rhizoma in Korean Pharmacopeia from other herbal plants using HPLC and TLC chromatograms was developed. The multivariate peak data of the chromatograms of methanol extracts of Rhei Rhizoma were used for hierarchical clustering analysis, principal components analysis and similarity calculation. Besides of the statistic analysis, TLC patterns of samples could be used as criteria of the discrimination. The developed pattern analysis method was specific and could be readily utilized for comprehensive evaluation of Rhei Rhizoma.

• Korean journal of food and cookery science
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• v.23 no.1 s.97
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• pp.99-106
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• 2007

This study investigated the effect of UV-B irradiation on the quality of Pleurotus ostreatus. The changes of vitamin $D_2$ contents, color value and flavor pattern in mushrooms were analyzed by high-performance liquid chromatography (HPLC), chromameter and gas chromatography - surface acoustic wave (GC-SAW) electronic nose. By exposure to UV-B irradiation (0 kj/m$^2$, 10 kj/m$^2$, 20 kj/m$^2$), vitamin $D_2$ content increased from 0 (control) to 48.50 g/g (DM: dry matter, 10 kj/m$^2$) and 61.58 g/g (DM, 20 kj/m$^2$). Although there was no significant difference in L, a, b values among the three groups, flavor changes were detected by GC-SAW electronic nose. The number of peaks increased from 10 in the control group (0 kj/m$^2$), to 14 and 15 for the 10 kj/m$^2$ and 20 kj/m$^2$ groups, respectively. Nevertheless, the changes of flavor pattern were not detrimental to the mushroom quality. These results suggested that UV-B irradiation is an effective method to increase the vitamin $D_2$ content without degrading the quality.

• Korean Journal of Food Science and Technology
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• v.24 no.6
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• pp.519-523
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• 1992

The patterns on the proteolysis of mussel protein using a commercial enzyme preparation were investigated. The best one among six commercial enzyme preparations for the manufacture of mussel extract was Corolase PP, based on the degree of hydrolysis (DH). When the raw mussel paste, without water addition, was adjusted to pH 6.5, added 0.1% (w/w dry basis) of Corolase PP. and reacted at $50^{\circ}C$ for four hours, it reached the maximum value of DH (79%). The precooking of raw mussel decreased the efficiency of extraction and hydrolysis of the protein, due to the inactivation of the autolytic enzymes contained in the mussel. During the course of proteolysis, major free amino acids such as glycine, alanine, glutamic acid and lysine, representing a characteristic brothy taste of mussel were replaced with free hydrophobic amino acids including valine, methionine, isoleucine, and leucine. The electrophoretic pattern and HPLC-GPC pattern of mussel protein hydrolysates during the hydrolysis were observed and also discussed.

• The Journal of Korean Medicine
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• v.27 no.4
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• pp.150-155
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• 2006

Object : This study investigated the quality inspection of Schizandra chinensis (Turcz.) Bail distributed in Korea. Methods: To evaluate the quality of these herbal medicines, we carried out TLC pattern analysis, purity, loss on drying, ash, acid-insoluble ash, essential oil contents, dilute ethanol-soluble, water-soluble, ether-soluble extracts and HPLC analysis. Results : As a result, TCL pattern analysis of schizandrin and gomisin A showed Rf=0.64 and 0.74, respectively. To measured content of schizandrin and gomisin A, we quantitatively analyzed using HPLC. The average contents of schizandrin and gomisin A were detected to be 0.60 (${\pm}$0.02)% and 0.12 (${\pm}$0.004)%, respectively. Discussion : As a result of this study, we suggest that the minimum content of schizandrin and gomisin A should be 0.5% and 0.1%, respectively. We could suggest that the minimum of essential oil content should be 0.6 ml.

• The Journal of Korean Obstetrics and Gynecology
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• v.33 no.2
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• pp.1-12
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• 2020

Objectives: This study was conducted to find out the optimal extraction time for Artemisia argyi. Methods: The compositional pattern was compared with HPLC (High Performance Liquid Chromatography) and GC (Gas-Chromatography) by decocting Artemisia argyi 10, 60, 120 minutes respectively. Results: With longer extraction time, the contents of reference compounds were extracted 1.1 times more when 3,4-dicaffeoylquinic acid was extracted for 60 minutes than when extracted for 10 minutes in HPLC test, but the contents were reduced when extracted for 120 minutes compared to 60 minutes extraction time. 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin showed the largest yield rate when extracted for 10 minutes, and it decreased as time passed. The contents of chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, jaceosidin, scoparone, and eupatilin were detected only in 10 minutes extraction but not in 60 or 120 minutes extraction according to GC test. Conclusions: The results show that extraction time could affect the physicochemical characteristic or composition of Artemisia argy extracted. Thus, short extraction time could be useful for decoction of Artemisia argyi.

• Journal of Acupuncture Research
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• v.27 no.2
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• pp.105-113
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• 2010

Objectives : This study was performed to analyze the quality of Ojeok-san extract powders distributed in Korea. Methods : HPLC analyses of Ojeok-san decoction produced in laboratory and 8 kinds of Ojeok-san extract powders were done. Also, quantity analyses of paeoniflorin in Paeonia lactiflora, Glycyrrhizin in Glycyrrhiza uralensis, alkaloid in Ephedra sinica, Hesperidin in Citrus unshiu Markovich, and cinnamic acid in Cinnamomum cassia Blume were performed to investigate the accurate content of 6 kinds of Ojeok-san extract powders. Results : In the pattern analysis, the peaks were compared. The content of components in herbal extract powders was 14.7 to 15.8% of that of decoction in average. The range was measured from 8.5 to 25.7%. The pattern comparison showed significant differences among the pharmaceutical companies' products. As for the quantity analysis, one of herbal extract powder contained insufficient amount of Paeonia lactiflora components and one of herbal extract powder was in short of Ephedra sinica components. All of the others contained more than standard component(over 90%). Conclusions : Because herbal extract powders are also medicine, standardized manufacturing methods and consistent quality management are necessary. Therefore exaction and operation of national standards and various researches to improve the quality management of herbal extract powders is urgent.

• The Korea Journal of Herbology
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• v.29 no.4
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• pp.61-67
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• 2014

Objectives : To present a differential standard of Armeniacae Semen and Persicae Semen that are easily confused. Methods : It was planed a differential standard form through outer appearance of the original plant, outer appearance in the form of each medicines and the appearance of the interior form through a microscope for each samples. Using an standard compound amygdalin, each samples have been analyzed by HPLC and TLC. Results : It was possible to distinguish the orignal plant between Armeniacae Semen and Persicae Semen through its leaf shape and calyx type. In Outer appearance in the form of each medicines, there was no difference pre-existing method(measuring length and width). In vascular pattern of the surface, however, there was a clear difference that Armeniacae Semen was developed more reticulated branches than Persicae Semen. In appearance through a microscope, it has not been possible to find a clear difference in the per original plant. However, there was a clear difference between Armeniacae Semen(1 layer) and Persicae Semen(3 layer) in inner albubemen cell. In TLC analysis, there was no difference in the pattern between samples. But in HPLC analysis, Armeniacae Semen showed amygdalin content higher on average. Conclusions : It will be possible to find various differences in the outer appearance of the original plant, the outer appearance in the form of each medicines, the appearance of the interior form through a microscope and physical and chemical research component.

• The Journal of Internal Korean Medicine
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• v.27 no.1
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• pp.55-71
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• 2006

Objectives : To find out more pharmacologically efficient way of extraction of herbal dicoction, Gamichungsangbohatang (GMCSBHT). Methods : Several main components of GMCSBHT was compared and analysed between hot water extract of GMCSBHT and Alcohol(70% ethanol) extract of GMCSBHT via HPLC method. Results : Hot water extract of GMCSBHT showed relatively more component content than ethanol extract of GMCSBHT. Also weighted mean of main components of hot water extraction of GMCSBHT was higher than that of alcohol extract of GMCSBHT. But from chromatographic pattern analysis of matching ratio and similarity ratio showed that these two forms of extracts might have different chemical composition, and 3D PDA plot of alcohol extract of GMCSBHT showed high peaks near UV $190{\sim}220nm$ which was invisible in hot water extract of GMCSBHT. Conclusion : Alcohol extract of GMCSBHT may have some special components which do not exist in hot water extract of GMCSBHT.