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Ribose-induced Maillard Reaction as an Analytical Method for Detection of Adulteration and Differentiation of Chilled and Frozen-thawed Minced Veal

  • Akbarabadi, Masoumeh;Mohsenzadeh, Mohammad;Housaindokht, Mohammad-Reza
    • Food Science of Animal Resources
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    • v.40 no.3
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    • pp.350-361
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    • 2020
  • Quality control of meat products is one of the main concerns of consumers, governmental control authorities, and retailers. The purpose of this study was to employ ribose-induced Maillard reaction in detection of meat adulteration and differentiation of fresh-chilled from frozen-thawed minced veal. The browning intensity was assessed through measuring the absorbance at 420 nm with a spectrophotometer as well as the direct analysis of the color and pH. The results showed that CIE b*, CIE a*, and A420* values in the extract of fresh-chilled veal were significantly (p<0.05) higher than frozen-thawed samples. The extract of frozen meat samples stored at -18℃ became significantly darker and more yellowish compared to -4℃. The results showed that the A420* value in the frozen-thawed veal stored at -4℃ and -18℃ was reduced by approximately 17.22±3.53% and 11.68±2.49%, respectively, compared with fresh-chilled veal. The findings also showed that the storage temperature of minced veal and the heating time in this reaction had a significant effect on all tested variables (p<0.0001). The proposed method can be considered as an easy, quick, and inexpensive test for differentiating between the fresh-chilled and frozen-thawed minced veal.

Study on Combustion Characteristics with Fuel Injection Timing in a RI-CNG Engine (RI-CNG 엔진에서 연료 분사시기에 따른 연소특성에 관한 연구)

  • Park, J.S.;Ha, D.H.;Yeum, J.K.;Ha, J.Y.;Chung, S.S.
    • Journal of Power System Engineering
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    • v.12 no.4
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    • pp.5-11
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    • 2008
  • The RI gasoline engine haying a sub-chamber had a high cycle variation due to the difficulty of the residual gas scavenge in the sub-chamber. To solve this problem and improve the combustion performance of RI engine, we devised a method to inject directly CNG fuel into the sub-chamber. A DI diesel engine of single cylinder was converted into a RI-CNG engine and an electronic control unit for the engine was manufactured. In this study, the combustion characteristics of the RI-CNG engine were investigated with the injection timings and air excess ratios at the load conditions of 50% throttle open rate and 1700rpm. As the results from this study, the RI-CNG engine worked reliably under the condition of the ignitable lean limit of $\lambda=1.7$ by showing the $COV_{imep}$ below about 5%. And the highest thermal efficiency could be obtained in the injection timing that produced the high imep and the low $COV_{imep}$ at the same time. The CO emission concentration indicated very low values and the THC and $NO_x$ showed an opposite pattern. With a view to improving the thermal efficiency and reducing the harmful emissions, the proper control region of the ignition timing and the mixture ratio were nearly ATDC $20^{\circ}\sim50^{\circ}$ and $\lambda=1.4$ respectively.

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New typhoid vaccine using sponge-like reduced protocol: development and evaluation

  • Rehab Bahy;Asmaa Gaber;Hamdallah Zedan;Mona Mabrook
    • Clinical and Experimental Vaccine Research
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    • v.12 no.1
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    • pp.70-76
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    • 2023
  • Purpose: Typhoid remains a major health problem, especially in the developing world. Furthermore, the emergence of multidrug-resistant and extensively drug-resistant strains of Salmonella typhi added a sense of urgency to develop more effective typhoid vaccines, one of which is bacterial ghosts (BGs), prepared by both genetic and chemical means. The chemical method includes incubation with numerous agents for a short time at their minimum inhibitory or minimum growth concentrations. This study included the preparation of BGs by a sponge-like reduced protocol (SLRP). Materials and Methods: Critical concentrations of sodium dodecyl sulfate, NaOH, and H2O2 were used. Moreover, high-quality BGs were visualized by scanning electron microscope (SEM). Subculturing was used to confirm the absence of vital cells. Besides, the concentrations of the released DNA and protein were estimated spectrophotometrically. In addition, the integrity of cells was proved by visualizing Gram-stained cells using a light microscope. Furthermore, a comparison between the immunogenicity and safety of the prepared vaccine and the available whole-cell killed vaccine was established. Results: Improved preparation of high-quality BGs of S. typhi, visualized by SEM, revealed punctured cells with intact outer shells. Moreover, the absence of vital cells was confirmed by subculturing. At the same time, the release of respective amounts of proteins and DNA is another evidence of BGs' production. Additionally, the challenge test provided evidence that the prepared BGs are immunogenic and have the same efficacy as the whole cell vaccine. Conclusion: The SLRP provided a simple, economical, and feasible method for BGs preparation.

Comparative Study on Phenolic Compounds of Cheorwon Onion by Phosphite Treatment (아인산염 처리에 따른 철원양파의 페놀화합물 비교 연구)

  • Kim, Y.B.;Lee, H.J.;Park, C.H.;Kim, D.H.;Koo, H.J.;Chang, K.J.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.2
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    • pp.105-114
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    • 2018
  • The aim of this study was to evaluate the change of phenolic compounds after phosphite treatment on Cheorwon onion. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is widely cultivated in the temperate regions of the world. Onion is a good name for the 'Okchong' to drop blood cholesterol and cardiovascular blood flow to increase the prevention of adult diseases. Cheorwon area is inland, but it has high continental climate due to its high altitude. Therefore it is said that the onion cultivated in this region has higher sugar content and higher taste than onion grown in the southern region. Phosphorus components are particularly important ingredients for promoting muscle development. However, if the phosphoric acid content of the soil part is maintained to a large extent until the harvest, the competition of the nutrients tends to cause decay of the root part. Therefore, it is important to improve the quality and shelf life of onion by inducing nutrient balance by applying foliar fertilization method on the reducing phosphorus at harvest time. In this study, acidity was controlled by diluting phosphorous acid(H3PO3) and potassium hydroxide(KOH), followed by leaf surface treatment with phosphite on onion. In this study, the concentration of phosphite was diluted to 500, 1,000, 1,500ppm and sprayed three times over the onion leaves in May 2018 using an atomizer and harvested at the end of June, and the phenolic compounds were analyzed by HPLC. As a result, the content of quercetin, one of the important substances in onion, was phosphite 500ppm(179.70㎍/g), 1,000(150.27), 1,500(105.95). The contents of caffeic acid, p-coumaric acid, ferulic acid, rutin, kaempferol, and sugar content were higher in the treatments than in the control. Therefore, the phosphite does not have a great influence on the growth, but it may play a role as a method of achieving balance with nitrogen in the rainy season by supplying the role of the material catalyst and the water soluble phosphoric acid and the potassium in the influence of the material change.

Car-door-controlled collision protection system using proximity sensor (근접센서를 이용한 차량 도어 제어 충돌 방지 시스템)

  • Lee S.H.;Cho H.S.;Heo J.K.;Lee J.H.;Kim W.K.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.10a
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    • pp.971-975
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    • 2005
  • In this study, a car-door-controlled collision protection system using proximity sensor is proposed and its preliminary analysis and several preliminary experiments are conducted. The proposed system has three additional sub-components on the car-door that is, a pair of extra electro-magnetic actuator that are attached to the sliding bar of the open/close car-door four-bar mechanism, a proximity sensor that would be attached to the outside surface of the door which is likely to frequently contact to the object and a driving control circuit of the whole system. A proximity sensor is used to detect object close to the car-door, the driving control circuit provides actuating power command to the electro-magnets to generate braking force to stop the swing motion of the car-door. It is verified through kinematic analysis of the four-bar car-door open/close mechanism and through experiments that the magnitude of maximum electronic magnetic force could provide the braking force enough for this application. For this purpose, an electro-magnet driving circuit is implemented and tested. And also to increase the safety of the system a time delay circuit is implemented and tested.

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Embedded Software Development for MSC on KOMPSAT-2

  • Heo, H.P.;Kong, J.P.;Yong, S.S.;Kim, Y.S.;Park, J.E.;Youn, H.S.;Paik, H.Y.
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.1093-1095
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    • 2003
  • MSC(Multi-Spectral Camera) system is a remote sensing instrument to obtain high resolution ground image. MSC system includes main control unit, called SBC(Single Board Computer). SBC controls all the sub-units of MSC system and communicates with spacecraft bus. The software developed for SBC should be reliable and autonomous to support various kinds of imaging missions. It is being developed using VxWorks real-time operating system to manage all tasks for all units efficiently. In this paper, the characteristics of the embedded software on the MSC system will be presented. It covers the hardware related characteristics like the BSP(Board Support Package), device driver and code patch mechanism.

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Electronic Structure of the SrTiO3(001) Surfaces: Effects of the Oxygen Vacancy and Hydrogen Adsorption

  • Takeyasua, K.;Fukadaa, K.;Oguraa, S.;Matsumotob, M.;Fukutania, K.
    • Applied Science and Convergence Technology
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    • v.23 no.5
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    • pp.201-210
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    • 2014
  • The influence of electron irradiation and hydrogen adsorption on the electronic structure of the $SrTiO_3$ (001) surface was investigated by ultraviolet photoemission spectroscopy (UPS). Upon electron irradiation of the surface, UPS revealed an electronic state within the band gap (in-gap state: IGS) with the surface kept at $1{\times}1$. This is considered to originate from oxygen vacancies at the topmost surface formed by electron-stimulated desorption of oxygen. Electron irradiation also caused a downward shift of the valence band maximum indicating downward band-bending and formation of a conductive layer on the surface. With oxygen dosage on the electron-irradiated surface, on the other hand, the IGS intensity was decreased along with upward band-bending, which points to disappearance of the conductive layer. The results indicate that electron irradiation and oxygen dosage allow us to control the surface electronic structure between semiconducting (nearly-vacancy free: NVF) and metallic (oxygen de cient: OD) regimes by changing the density of the oxygen vacancy. When the NVF surface was exposed to atomic hydrogen, in-gap states were induced along with downward band bending. The hydrogen saturation coverage was evaluated to be $3.1{\pm}0.8{\times}10^{14}cm^{-2}$ with nuclear reaction analysis. From the IGS intensity and H coverage, we argue that H is positively charged as $H^{{\sim}0:3+}$ on the NVF surface. On the OD surface, on the other hand, the IGS intensity due to oxygen vacancies was found to decrease to half the initial value with molecular hydrogen dosage. H is expected to be negatively charged as $H^-$ on the OD surface by occupying the oxygen vacancy site.

Induction of Apoptotic Cell Death by Methanol Extract of Houttuynia cordata Thunb. in A549 Human Lung Carcinoma Cells (어성초 메탄올 추출물에 의한 A549 인체 폐암세포 사멸유도에 관한 연구)

  • Hong, Su-Hyun;Park, Cheol;Hong, Sang-Hoon;Choi, Byung-Tae;Lee, Yong-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.6
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    • pp.1584-1592
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    • 2006
  • Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is traditional medicinal plant generally used in Oriental medicine therapy. We previously reported that the water extract of H. cordata inhibited cell proliferation and induced apoptosis in human breast carcinoma cells. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in human lung carcinoma A549 cells. It was found that MEHC could inhibit the cell growth in a dose-dependent manner, which was associated with morphological change and apoptotic cell death as determined by formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase cells. Apoptosis of A549 cells by MEHC was also connected with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. MEHC treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), ${\beta}$-catenin and phospholipase (PLC)-${\gamma}$1 protein expression. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

Current and Future Prospects for Insect Behavior-modifying Chemicals in China

  • Du, Jia-Wei
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.222-229
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    • 2000
  • In this paper we will assess the feasibility of some insect behavior-modifying chemicals for insect control, such as male orientation inhibitor, female calling interrupter and female attractant of cotton bollworm Helicoverpa armigera. These behavior-modifying chemicals have advantages of simple chemical structure, easy to synthesize and low price of products. The effects of sub-lethal insecticides on insect chemical communication system and the differences of pheromone communication systems between the resistant and susceptible strain of H. armigera will also be discussed.

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A Study on the Effect of Improving Permeability by Injecting a Soil Remediation Agent in the In-situ Remediation Method Using Plasma Blasting, Pneumatic Fracturing, and Vacuum Suction Method (플라즈마 블라스팅, 공압파쇄, 진공추출이 활용된 지중 토양정화공법의 정화제 주입에 따른 투수성 개선 연구)

  • Geun-Chun Lee;Jae-Yong Song;Cha-Won Kang;Hyun-Shic Jang;Bo-An Jang;Yu-Chul Park
    • The Journal of Engineering Geology
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    • v.33 no.3
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    • pp.371-388
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    • 2023
  • A stratum with a complex composition and a distributed low-permeability soil layer is difficult to remediate quickly because the soil remediation does not proceed easily. For efficient purification, the permeability should be improved and the soil remediation agent (H2O2) should be injected into the contaminated section to make sufficient contact with the TPH (Total petroleum hydrocarbons). This study analyzed a method for crack formation and effective delivery of the soil remediation agent based on pneumatic fracturing, plasma blasting, and vacuum suction (the PPV method) and compared its improvement effect relative to chemical oxidation. A demonstration test confirmed the effective delivery of the soil remediation agent to a site contaminated with TPH. The injection amount and injection time were monitored to calculate the delivery characteristics and the range of influence, and electrical resistivity surveying qualitatively confirmed changes in the underground environment. Permeability tests also evaluated and compared the permeability changes for each method. The amount of soil remediation agent injected was increased by about 4.74 to 7.48 times in the experimental group (PPV method) compared with the control group (chemical oxidation); the PPV method allowed injection rates per unit time (L/min) about 5.00 to 7.54 times quicker than the control method. Electrical resistivity measurements assessed that in the PPV method, the diffusion of H2O22 and other fluids to the surface soil layer reduced the low resistivity change ratio: the horizontal change ratio between the injection well and the extraction well decreased the resistivity by about 1.12 to 2.38 times. Quantitative evaluation of hydraulic conductivity at the end of the test found that the control group had 21.1% of the original hydraulic conductivity and the experimental group retained 81.3% of the initial value, close to the initial permeability coefficient. Calculated radii of influence based on the survey results showed that the results of the PPV method were improved by 220% on average compared with those of the control group.