• Journal of the Korean Electrochemical Society
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• v.10 no.2
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• pp.150-154
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• 2007

Redox complexes to transport electrodes from biomaterial to electrodes are very important part in commercial biosensor industry. A novel osmium redox complex was synthesized by the coordinating pyridine group with osmium metal. A novel osmium complex is described as $[Os(dme-bpy)_2(ap-im)Cl]^{+/2+}$. We have been studied the electrochemical characteristics of this osmium complex with electrochemical techniques such as cyclic voltammetry and chronoamperommetry. In order to immobilize osmium redox complexes on the electrode, we deposited gold nano-particles on screen printed carbon electrode(SPE). The electrical signal converts the osmium redox films into an electrocatalyst for glucose oxidation. The catalytic currents were monitored that the catalytic currents were linearly increased from 1 mM to 5 mM concentrations of glucose.

• Journal of Biosystems Engineering
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• v.31 no.5 s.118
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• pp.443-448
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• 2006

For development of an on-line monitoring unit of fermentation process, an auto dilution unit based on traditional chemical and biological analytical method was developed and the performance was evaluated. The dilution unit was constructed with two syringe pumps and flow direction change valves and fully automated. Total delivery volume of two pumps using distilled water was measured to confirm the operating stability And diluted concentrations of three substrate solutions (glucose, lactic acid, ethanol) were compared with a standard method with a high performance liquid chromatograph (glucose, lactic acid) and gas chromatograph (ethanol). Relative error values of total delivery volume of the pumps were below 3% and standard deviation values were 0.003 (n=5). Relative error values of diluted concentration of the dilution unit measurements were below 2% with 1/10 of dilution ratio and 70, $80{\mu}{\ell}$ of sample volume conditions for glucose and lactic acid, 1/30 of dilution ratio and $70{\mu}{\ell}$ of sample volume conditions for ethanol, respectively. In case of the ethanol, cause of the evaporative characteristics, the relative error values showed over 5% whole experimental conditions.

• Applied Chemistry for Engineering
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• v.24 no.2
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• pp.113-120
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• 2013

Due to human life expectancy of the recent development of medical technology recently, it leads to increase the desire for improving the quality of human life, and grow health concerns and needs. Therefore, in order to prevent the occurrence of disease and to check up a disease quickly, research on the development of a biosensor has been actively processed. One of them, the nano-carbon materials, are very suitable for manufacturing biosensor due to their excellent electrical/mechanical properties. In this review, we introduced the recent studies about preparation methods of carbon electrodes using the carbon nano-materials for biosensors as well as its technological applications.

• Korean Journal of Materials Research
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• v.25 no.12
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• pp.655-658
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• 2015

The development of glucose biosensors has been attracting much attention because of their importance in monitoring glucose in the human body; such sensors are used to diagnose diabetes and related human diseases. Thanks to the high selectivity, sensitivity to glucose detection, and relatively low-cost fabrication of enzyme-immobilized electrochemical glucose sensors, these devices are recognized as one of the most intensively investigated glucose sensor types. In this work, ZnO nanofibers were synthesized using an electrospinning method with polyvinyl alcohol zinc acetate as precursor material. Using the synthesized ZnO nanofibers, we fabricated glucose biosensors in which glucose oxidase was immobilized on the ZnO nanofibers. The sensors were used to detect a wide range of glucose from 10 to 700 M with a sensitivity of $10.01nA/cm^2-{\mu}M$, indicating that the ZnO nanofiber-based glucose sensor can be used for the detection of glucose in the human body. The control of nanograins in terms of the size and crystalline quality of the individual nanofibers is required for improving the glucose-sensing abilities of the nanofibers.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1536-1537
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• 2007

본 논문은 당뇨병의 지표 물질인 glucose의 농도를 극미량의 시료를 사용하여 정량할 수 있는 방법을 개발하기 위해 organic/inorganic 네트웍에 의해 안정화된 나노입자 효소를 이용하여 초소형 효소 전극을 개발하였다. 전극은 실리콘 웨이퍼상에 반도체 공정을 이용하여 마이크로 크기의 금 박막 전극을 제작하였다, Organic/Inorganic 물질과 함께 합성된 glucose oxidase 나노입자는 20nm 크기로 투과형 전자현미경 (Transmission Electron Microscope:TEM)으로 관찰하고, 푸리에변환 적외선분광법(Fourier transform infrared spectrophotometer : FTIR) 을 이용하여 분석하였고, 전극 특성을 알아보기 위해 Potentiostat/Galvanostat을 사용하여 전기 화학 실험을 하였다. 제작된 전극은 시간대 전류법으로 glucose의 농도에 따른 감도를 측정하였다. 실험결과에 따라 전극의 표면에서 발생하는 전류는 glucose의 농도에 비례함을 알 수 있었다. 또한 순환 전압전류법을 통하여 감도를 측정하였다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.173-174
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• 2011

• Applied Chemistry for Engineering
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• v.26 no.1
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• pp.47-52
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• 2015

To improve both the GOD immobilization capability and sensitivity of MWCNTs-based biosensor electrode, the electrode was prepared by adding different quantities of GO. The addition of GO increased hydrophilicity and the surface free energy of electrodes for glucose sensing as well as the dispersion of MWCNTs. In addition, the GOD immobilization capability was enhanced and the sensitivity was improved up to $121{\mu}A\;mM^{-1}$ even though having a high $K_m$ value (0.105) when adding 0.05 g GO to 0.05 g MWCNTs. These experimental results were attributed to the fact that the improvement in dispersion stability for MWCNTs, hydrophilicity, and surface free energy of electrode surface due to the addition of GO affected GOD immobilization capability.

• Korean Chemical Engineering Research
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• v.59 no.1
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• pp.100-105
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• 2021

This paper proposes the polydimethylsiloxane (PDMS) blade coating method for fabrication of paper-based analytical device (PAD) that is able to monitor the disease diagnosis and progress without special analytical equipment. The mold that has PAD design is easily modified by using laser cutting technique. And the fabricated mold is used for hydrophobic barrier formation by blade coating. We have optimized the stable formation of PDMS hydrophobic barrier as blade coating condition, which is established by analyzing the structure of the PDMS hydrophobic barrier and change of hydrophilic channel size as thickness of the ink and contact time with the chromatography paper. Based on optimal condition, we demonstrate that PAD as biosensor can apply to detect protein, glucose, and metal ion without special analysis equipment.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.595-601
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• 2017

In vivo assay of glucose detection was described using a skin tattoo film electrode (STF), and the probe was made from carbon nano tube paste modification film paper. Here in the square-wave stripping anodic working range obtained of $20-100mgL^{-1}$ within an accumulation time of 0 seconds only in sea water electrolyte solutions of pH 7.0. The relative standard deviations of 50 mg glucose that were observed of 0.14 % (n=12), respectively, using optimum stripping accumulation of 30 sec, the low detection limit (S/N) was pegged at 15.8 mg/L. The developed results can be applied to the detect of in vivo skin sensing in real time. Which confirms the results are usable for in vitro or vivo diagnostic clinical analysis.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1379-1385
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• 2018

The hand held voltammetry systems searched diabetic assay using glucose sensor of fluorine nafion doped carbon nanotube electrode (FCNE). An inexpensive graphite carbon pencil was used as an Ag/AgCl reference and Pt counter electrode. Upon combining and using three electrode systems, optimum square wave (SW) stripping results were attained to 1.0-9.0 ug/L with 8 points. Statistic RSD precision was of 6.02 % with n=15 in 0.1 mg/L glucose. After a total of 200 second accumulation times, analytical detection limit of 0.8 ug/L was obtained. This developed technique was applied to urine samples from diabetic patients urine for fluid analysis, it was determined that the sensor can be used with a diagnostics in the ex vivo of live cells and non treated biological fluid.