• 한국물환경학회지
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• 제26권2호
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• pp.215-224
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• 2010

This study was carried out to estimate effects of sediment reduction with net type sediment settling pond at highland agricultural areas. Flow and sediment monitoring system with rainfall gauge were installed at the study area for real-time monitoring and analysis. It was found that amount and intensity of rainfall events strongly affect sediment yield from the study area. With net type sediment settling pond, it was expected that approximately 61% of sediment could be removed. The correlation between heavy metals and SS were analyzed in this study. $R^2$ values were 0.644, 0.876, 0.945, and 0.928 for Fe, Mn, Al, and Si, respectively. This results indicate that heavy metals also could be removed with net type sediment settling pond. As shown in this study, the sediment settling pond will be an efficient sediment reduction facility at highland agricultural areas. For maximum reduction efficiencies of sediment and other pollutants at agricultural fields, simplified sediment settling pond should be designed and implemented.

• 한국약용작물학회지
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• 제17권4호
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• pp.233-237
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• 2009

Astragalus membranaceus has a long history of medicinal use in Chinese herbal medicine. It has been shown to have immunostimulant, tonic, antioxidant, antiperspirant, diuretic, anti-diabetic, expectorant properties, and a supplementary medicine during cancer therapy. In this study, we investigated the effect of anti-oxidation of Astragalus membranaceus root extract. The anti-oxidative activities of water, 80% methanol, and 100% methanol extracts from Astragalus membranaceus were analyzed by DPPH free radical scavenging activity, Superoxide dismutase-like activity, reducing power, and crude ash. The water extract demonstrated to be more effective than methanol extract for a DPPH radicals scavenging activities and reducing power. Superoxide dismutase-like activity showed higher efficiency in 80% methanol extract. Our results indicate that Astragalus membranaceus extracts could be used as a source of antioxidant ingredients in the food industry.

• Journal of Applied Biological Chemistry
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• 제52권4호
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• pp.216-220
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• 2009

Aldo-keto reductase family 1 B10 (AKR1B10) has been considered as a potential cancer therapeutic target. Ethanol extracts prepared from 82 Korean local plants were examined for their antioxidant activity and inhibitory effects on recombinant human AKR1B10 (rhAKR1B10) in vitro. 21 extracts showed more than 80% of ABTS radical scavenging activity at $100\;{\mu}g/mL$ and 11 extracts inhibited more than 50% of rhAKR1B10 activity at $10\;{\mu}g/mL$. Especially, 9 extracts showed potent inhibition on rhAKR1B10 activity compared with positive control tetramethylene glutaric acid.

• 생약학회지
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• 제40권3호
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• pp.238-243
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• 2009

We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of $10{\mu}g$/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their $IC_{50}$ values were 2.94 and $2.00{\mu}g$/ml, respectively.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.133-144
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• 2014

This study was carried out to review the pattern of forest stand development for six Gangwondo Baekdudaegan Mountains which experienced different type and duration of intensive legal protection. Vegetation data from point sampling method were employed to classify community types by cluster analysis on the basis of the importance values of canopy tree species for the study areas. The names of classified communities were given by the composition of dominant tree species. The communities were also compared one another in terms of stand structure by species diversity index. The results indicated that National Parks (Seoraksan and Odaesan) had greater proportion of mixed mesophytic forest type which was supposed to progress further forest succession process so as to have more complex and diversified stand structure. On the other hand, ordinary forest areas (Seokbyeongsan and Deokhangsan) had greater proportion of the forest types which was dominatively composed of Quercus mongolica and Pinus densiflora. The forest types with large amount of these two species would tend to develop for relatively short period of time of 40-50 years after artificial disturbances. Hyangnobong of Natural Protection Area and Hambaeksan of Natural Ecosystem Conservation Area showed intermediate stand development pattern in between National Parks and ordinary forest areas. The period of intensive legal protection of the forest area was positively correlated with species diversity index (R=0.736), and noted that the forest which received intensive protection regulation for longer period tended to show more complex and diversified stand structure.

• 환경생물
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• 제31권3호
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• pp.225-231
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• 2013

동해안의 암반조간대 무척추동물상의 분포 특성을 연구하기 위하여 독도를 비롯한 울릉도, 경상북도의 경주, 포항, 영덕, 울진 그리고 강원도 등 총 19개 정점의 암반 조간대무척추동물상을 조사하여 출현종의 유사도를 공통종의 출현비율 (%)과 Bray-Curtis similarity matrix를 이용한 집괴분석과 MDS를 이용하여 분석하였으며 SIMPER를 이용하여 독도와 그 외의 동해안 암반저서 무척추동물의 특징종을 선출하였다. 공통종의 출현비와 집괴분석결과, 가장 가까이 위치한 울릉도를 제외하면, 경상북도의 영덕이 가장 높은 유사도를 보였으나, 전체적으로 독도 암반저서생태계는 다른 동해안 연안과 차이를 보인 것으로 나타났다.

• 한국수정란이식학회지
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• 제27권4호
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• pp.259-264
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• 2012

Solution of glycerol, ethylene glycol, sucrose, dextrose (GESD) and cryotop methods were carried out to investigate the survivability on vitrification of embryos. Embryos cultured in vitro were vitrified by GESD of 10 or 8 step and cryotop methods of 6 step, from cryopreservation step to frozen-thawed and culture step. Survival rate and ICM, TE cells of embryos were investigated after frozen-thawed 24 h. As a results, cryotop method was significantly (p<0.05) higher ($85.76{\pm}5.3$ vs. $66.71{\pm}2.4$, $44.80{\pm}2.1%$) than GESD 10 or 8 step methods on survivability. Also, In ICM cell number, cryotop method was significantly (p<0.05) higher to $45.67{\pm}4.7$ cells than GESD 8 step method. TE cell number was significantly (p<0.05) highest to $111.00{\pm}11.0$ cells in cryotop method. On the other hand, survival rate, TE and total cell number were all the significantly (p<0.05) high, except ICM in GESD 10 step method between GESD 10 step method and GESD 8 step method. In conclusion cryotop method was to be most effective, but it is considered necessary to study vitrification method for step-by-step freezing and thawing process.

• 한국수정란이식학회지
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• 제27권4호
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• pp.253-258
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• 2012

This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozen-thawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h ($94.2{\pm}2.6%$, $94.67{\pm}3.4$ and $129.67{\pm}5.5$). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to $98.3{\pm}1.7%$ after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

• Journal of Forest and Environmental Science
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• 제29권3호
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• pp.226-236
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• 2013

Recently check dam construction number have been increased by becoming known that effectiveness in the control of the landslide and debris flow. However, check dam management standards are not setting yet. Therefore, this study was carried by provide a basic data for the check dam management and inspection in the Gangwondo. The followings are the results of safety inspection on the 274 check dams, which are located in mountain streams, Gangwondo, Republic of Korea. 1. It was determined that, of 274 check dams inspected, 267 check dams (97.4%) generally had a safe condition but 7 check dams (2.6%) had a bad condition that repair and/or complementary measures are required. 2. The check dams with a bad condition had the ages greater than approximately 20 years. This result should be reflected to future stream management strategy including a timing of the specific inspection for durability of check dams. 3. Our finding suggests that the safety inspection of check dams is able to provide basic information required to maintain their own functions, and thus it should be widely applied to stream management strategy against to sediment-related disasters in the future.

• 자원ㆍ환경경제연구
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• 제20권4호
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• pp.797-826
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• 2011

본 연구는 RPS 도입시 재생에너지 전력에 대한 소비자의 지불의사가 지역별로 달라지는지 조건부가치평가법(CVM)을 적용하여 분석하였다. 서울, 울산, 전라남도, 강원도의 총 600명의 응답자를 대상으로 재생에너지 전력에 대한 지불의사액(WTP)을 도출한 결과, WTP 평균값은 서울 860.3원/월, 울산 1,677.3원/월, 전라남도 837.0원/월, 강원도 1,830.9원/월 수준으로 나타났다. 이 때, 전체 표본의 WTP 평균값과 개별 지역 표본의 WTP 평균값이 다르지 않다는 귀무가설을 기각하여, 지역별로 재생에너지 전력에 대한 선호가 달라짐을 보였다. 또한 재생에너지 전력에 대한 지역별 WTP의 차이는 저항응답 비율, 재생에너지 시설 인근 경험 및 재생에너지 시설 입지에 관한 수용성 등의 지역별 차이에서 기인하는 것으로 분석되었다.