• The Journal of Korean Society of Virology
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• v.28 no.1
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• pp.21-30
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• 1998

Synthetic genes encoding the gag p24 and the part of the envelope protein gp41 of the human immunodeficiency virus (HIV-1) were cloned and overexpressed as fusion proteins in Escherichia coli, using an expression vector carrying T7 promoter and the poly-histidine leader, sequence. The overexpressed p24 fusion protein was purified by centrifugation, Ni-affinity chromatography and CM-sepharose chromatography. The overexpressed gp41 fusion protein was purified by centrifugation, $C_4$ chromatography and DEAE-sepharose chromatography. The purified fusion proteins showed a high level of purity and immunoreactivity in SDS-polyacrylamide gel electrophoresis and western blot analysis. These results suggest that this prokaryotic - expression purification method is suitable for obtaining a large amount of the viral antigen which may be useful for screening of antibodies to HIV-1 in human blood samples.

• Advances in concrete construction
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• v.10 no.5
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• pp.417-429
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• 2020

Waste glass is a global solid waste with huge reserves. The discarded waste glass has caused a series of problems such as resource waste and environmental pollution, so it is urgent to recycle waste glass with high replacement level. Glass powder (GP), as a supplementary cementitious material (SCM), used in cement-based materials has already become one of the important ways to recycle waste glass mainly attributed to its pozzolanic reaction and filling effect, especially to the suppressed ASR expansion. This paper demonstrates an overview of the properties of GP and its effect on the fresh and mechanical properties of cement-based materials. The study found that the influence of GP on the performance of cement-based materials mainly depends on its content, particle size, color and type, curing conditions, and other SCMs. Finally, based on the problems involved in the investigation of concrete containing GP, some corresponding suggestions and efforts are given to further guide the utilization of GP in cement-based materials.

• Journal of IKEEE
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• v.15 no.3
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• pp.248-254
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• 2011

Recently, processing burden of CPU is growing because of graphical user interface according to enhance the performance of mobile devices and various graphical effects and creation of contents with 3D graphical effect or Flash animation. Therefore, the GPU are introduced to mobile device for support to variety contents. In this paper, OpenVG accelerator was implemented based on multi-core GP-GPU. OpenVG accelerator is verified using the sample image provided by Khronos group, and overall function is processed by only instruction set without dedicate hardware. The performance of processing the Tiger Image was 2 frames/sec.

• Spatial Information Research
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• v.13 no.1 s.32
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• pp.19-29
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• 2005

In this study, Real Time Kinematic GPS(RTK GPS) was conducted twice at the same site in two different seasons, respectively to check the possibility of it as the mapping tool, and how the factor affecting the accuracy of it. As a result, most parts of a small garden except f3r the worst environments surrounded with lots of tree canopy and several buildings were mapped using RTK GPS even in spring, full of a green foliage and winter as well. However, the mapping accuracy and the availability of RTK GPS were not so high. The study showed that it is recommended in RTK GPS mapping to utilize Total Station, etc. in the worst urban environments unable to track the satellite signals with ease.

• Journal of Korean Society for Geospatial Information Science
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• v.15 no.4
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• pp.41-50
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• 2007

This study aims to suggest efficient setting up of GPS (Global Positioning System) for cadastral surveying. In order to perform it effectively, worldly used modern surveying technology GPS introduced. In a practical analysis, GPS observation had been performed in the research area and the results had been compared and analysed with the results of Total Station. As a results, it is shown that GPS is more efficient rather than current surveying method in terms of time, cost and economy. The results of GPS surveying were included in the scope of error tolerance, being accepted by cadastral laws, showing high accuracy of GPS surveying method. With the results of analysis, how to application of GPS for cadastral surveying was suggested.

• 한국지형공간정보학회:학술대회논문집
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• 2002.03a
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• pp.17-22
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• 2002

인공위성을 이용한 위치결정시스템인 GPS(Global Positioning System)는 mm 단위의 정밀도로 정적, 동적 위치관측이 가능한 시스템으로 현재 여러 분야에서 활용이 증가하고 있다. 특히 교량, 댐 등 안전이 요구되는 사회기반 시설물의 동적 거동을 기록하고 감시하는데 있어서 GPS가 새로운 관측시스템으로 주목받고 있으며 이를 실용화하기 위한 연구가 활발히 진행되고 있다. 본 연구에서는 GPS를 이용하여 건물의 거동상태를 점검 하는 것을 목적으로 하고 있다. 계절풍에 의한 초고층건물의 거동을 해석하기 위하여 건물 옥상에 GPS, 가속도계, 풍향계, 풍속계를 설치하였다. GPS와 가속도계로를 이용하여 건물의 변위 및 가속도를 5Hz로 관측하였다. GPS를 이용한 변위관측은 1시간, 1분, 1초 등의 간격으로 3차원 변위를 계산하였으며, 계산된 3차원 변위를 가속도계, 풍속계, 풍향계와 연계하여 해석하였다. 대상 건물에 대한 최대 변위를 관측한 결과 계절에 따라 큰 차이를 보였으며 건물의 안정성을 평가할 수 있는 척도인 건물의 고유 진동수의 경우 약 0.2Hz의 값을 얻을 수 있었다. 향후 모든 센서들을 통합하여 실시간으로 건물을 모니터링할 수 있는 시스템을 개발할 경우 건물의 안정성 해석 및 유지보수에 본 연구가 큰 기여를 할 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.318-322
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• 1999

A new baculovirus transfer vector (pPGP404) was constructed to increase the expression level of human thrombopoietin (hTPO) in insect cells. In pPGP404, hTPO was cloned next to the AcNPV polyhedrin-gp64 dual promoter and the leader sequence of hTPO was substituted with that of gp64. A recombinant baculovirus, AcPGP404, was constructed by using pPGP404 as a transfer vector. hTPO was expressed in AcPGP404-infected TN5 cells and it was observed that the expression levels of hTPO in TN5 cells increased three-fold ($6.0 {\mu}g/ml^{-1}$) compared to the level expressed under the control of the polyhedrin single promoter. These results indicate that the polyhedrin-gp64 dual promoter system would be useful for expression in large quantities of recombinant proteins in insect cells.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.17 no.4
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• pp.373-381
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• 1999

Mobile Mapping System can be defined as vehicle mapping system which collects rapidly spatial data by integrated Gps/digital imaging system. Kinematic positioning by GPS is essential technology of Mobile Mapping System. This paper aims at analysing the accuracy and efficiency of kinematic positioning by GPS platformed on moving vehicle. For the purpose, roads were surveyed by vehicle/kinematic GPS. The results show that vehicle/kinematic GPS can measure spatial position faster, and still maintain a reasonable accuracy. But inertial navigation system and GPS should be integrated to compute continuous vehicle track and overcome gaps by blocked satellite signals for the more accurate positioning.

• The Journal of Natural Sciences
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• v.8 no.2
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• pp.35-41
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• 1996

To find the substrate interacting site of the MCAT1, charged amino acid residues in the transmembrane domain were changed to opposite charged amino acids and studied the arginine uptake, gp70 binding, efflux and protein expression using the Xenopus oocyte expression method. Among the five mutants of MCAT1, the D403K showed the most interesting characteristics, which had normal gp70 binding but low arginine uptake function, that means the normal expression on the membrane but decreased transport function. All mutants except K211E showed decreased arginine efflux, and kinetic study showed decreased Vmax. Together, Clu(403) residue of MCAT1 may show the possible substrate interacting site in the transmembrane domain of MCAT1.

• Journal of the Korean Institute of Intelligent Systems
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• v.20 no.6
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• pp.780-785
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• 2010

This paper introduces GP(Genetic Programming) based color detection model for an object detection and tracking. Existing color detection methods have used linear/nonlinear transformatin of RGB color-model and improved color model for illumination variation by optimization or learning techniques. However, most of cases have difficulties to classify various of colors because of interference of among color channels and are not robust for illumination variation. To solve these problems, we propose illumination robust and non-parametric multi-colors detection model using evolution of GP. The proposed method is compared to the existing color-models for various colors and images with different lighting conditions.