• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1665-1672
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• 2012

An 80% ethanol extract of Hizikia fusiforme was obtained and followed by successive fractionation using the organic solvents n-hexane, ethyl acetate, and n-butanol to identify the antioxidative substance. The aqueous part of the nbutanol fractionation step, showing high antioxidative activity, was subjected to reverse-phase liquid chromatography. As a result, a substance purified from a BB-2 fraction showed high antioxidative activity. The m/z 419 [M+H] molecular ion peak in the fraction was observed by the analysis of the ESI-LC/MS spectrum. By the analysis of 1H NMR (500 MHz, DMSO-$d_6$) and $^{13}C$ NMR (125 MHz, DMSO-$d_6$) spectra, a unique compound of the fraction was biochemically identified as a 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5HHMF). We also investigated the effect of 5HHMF on human gastric AGS carcinoma cells. Western blot analysis suggested that the flavone substantially increased the levels of the death receptor-associated apoptosis mediators Fas, Fas L, FADD, TRADD, and DR4 in a concentration-dependent manner. The levels of Fas, Fas L, TRADD, and DR4 in the cells treated with 5HHMF ($5{\mu}g/ml$) were approximately 26.4-, 12.8-, 6.7-, and 9.8-times higher than those of non-treated cells, respectively. Of note, the level of FADD protein in the cells exposed to 5HHMF ($1{\mu}g/ml$) increased approximately 9.6-times. In addition, the cleavage of caspase-3, -8, and -9 in cultured AGS cells treated with 5HHMF was significantly confirmed. Therefore, our results suggest that 5HHMF from H. fusiforme is involved in the induction of death receptor-associated apoptosis mediators in human gastric AGS carcinoma cells.

• Journal of Life Science
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• v.29 no.9
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• pp.1010-1015
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• 2019

The interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal (GI) tract. In the present study, the effects of olanzapine, an atypical antipsychotic agent, on pacemaker potentials in cultured ICCs from the small intestine of the mouse were investigated. The whole-cell patch-clamp configuration was used to record pacemaker potentials from cultured ICCs. Olanzapine produced pacemaker depolarizations in a concentration-dependent manner in current clamp mode. Methoctramine, a muscarinic $M_2$ receptor antagonist, did not inhibit olanzapine-induced pacemaker depolarizations, whereas 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) muscarinic $M_3$ receptor antagonist did inhibit it. When guanosine 5'-[${\beta}$-thio] diphosphate (GDP-${\beta}$-S; 1 mM) was in the pipette solution, olanzapine-induced pacemaker depolarization was blocked. Also, low $Na^+$ solution externally eliminated the generation of pacemaker potentials and inhibited the olanzapine-induced pacemaker depolarizations. Additionally, the nonselective cation channel blocker, flufenamic acid, inhibited the olanzapine-induced pacemaker depolarizations. Pretreatment with U-73122, an active phospholipase C (PLC) inhibitor, also eliminated the generation of pacemaker potentials and suppressed the olanzapine-induced pacemaker depolarizations. These results suggested that olanzapine modulates the pacemaker potentials through muscarinic $M_3$ receptor activation by G protein-dependent external $Na^+$ and PLC pathway in the ICCs. Therefore, olanzapine could affect intestinal motility through ICCs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.11
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• pp.1848-1856
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• 2013

This study is designed to examine the change in physical properties of extruded brown rice-vegetable mix at different temperatures and $CO_2$ gas injections. Moisture content and screw speed were fixed to 27% and 100 rpm respectively. Die temperatures and $CO_2$ gas injections were adjusted to 60, 80, $100^{\circ}C$ and 0, 150 mL/min, respectively. The ratio of ${\alpha}$-brown rice, brown rice and sugars (oligosaccharides and palatinose) was fixed to 25, 50 and 16%, respectively. Green tea, tomato and pumpkin powder were blended individually at 9%. Specific mechanical energy (SME) input decreased as die temperature for each vegetable addition increased. All extrudates decreased in density and breaking strength, but increased in specific length and water soluble index as $CO_2$ gas injection increased. Elastic modulus decreased as the die temperature and $CO_2$ gas injection increased. Extruded green tea mix with $CO_2$ gas injection at 150 mL/min was larger pore size and higher amount of pore than the tomato and pumpkin extrudates with $CO_2$ gas injection. Cold extrusion with $CO_2$ gas injection at $60^{\circ}C$ die temperature could be applicable for making Saengsik (uncooked food).

• Korean Journal of Breeding Science
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• v.41 no.1
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• pp.56-60
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• 2009

A new naked oat cultivar Daeyang (Avena sativa L.) was developed by Department of Rice and Winter Cereal Crop, NICS, RDA in 2007. It was derived from a cross between 'FLX446-1-84-Q1'. and 'SO92004-B-3-3-5-7'. The FLX446-1-84-Q1, a naked oat cultivar from USA, is early heading and has good seed quality, while the SO92004-B-3-3-5-7, a covered oat breeding line, has a high yield with large grain. Subsequent generations were handled in a bulk method and pedigree selection program, and the SO97013-B-16-4 was selected based on agronomic performance in 2001. The line showed both high yield and good husking rate of seed in the yield trial tested at Suwon from 2002 to 2003, being designated as Gwiri51. The Gwiri51 was subsequently evaluated for winter hardiness, earliness, and yield in four locations, Gimje, Iksan, Jeongeup, and Jinju, from 2004 to 2007 and was designated as "Daeyang" and released. Its heading date was May 8 and maturing time was June 14 in a paddy field condition. The new cultivar Daeyang had 97 cm of culm length and 25.2 cm of spike length, 644 spikes per $m^2$, 65 grains per spike, 30.3 g of 1,000-grain weight, and 635 g of test weight. Daeyang showed better winter hardiness than that of the check cultivar 'Sunyang', and similar seed quality to the check cultivar in respect to percent content crude protein and $\beta$-glucan. However, it showed higher husking rate than the check cultivar. Grain yield of Daeyang in the regional yield trial for 4 years were averaged 4.18 MT $ha^{-1}$, which was 20% higher than that of the check cultivar Sunyang. Fall sowing cropping is recommended only in a south area where daily minimum mean temperatures are averaged higher than $-4^{\circ}C$ in January, and should be excluded in mountain area where frost damage is presumable.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.28 no.1
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• pp.80-98
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• 2002

Taxus cuspidata Sieb selected cultivation as drug, food and decorative plant in Kyong-gi province in Korea. As a manufacturing method, there were extracted from 250g of dried-leaf and 300g of dried-stem with each 200g of BG, PG and water (to 100) mixing for 72 hour at 50$\pm$5$\^{C}$ and then they were filtered by 400-mesh filter. Appearance of extract of leaves was slight brown, pH=5.3$\pm$0.5, gravity was 1.012$\pm$0.05, and a reflective index was 1.375$\pm$0.05. And appearance of extract of stems was slightly dark brown, pH=5.4$\pm$0.5, gravity was 1.016$\pm$0.05, and a reflective index was 1.358$\pm$0.05. It was extracted oil from Taxus seed. Gravity was 0.922$\pm$0.05 and it should be obtained the 27.0$\pm$0.5% of yield. The molecular weight of polysaccharide was about 50,000 to 300,000 dalton and contained 5.0$\pm$1.2% of yield from Taxus fruit. The determinations of total polyphenols in measuring spectropotometer got 0.563% in leaves, and 0.325% in stems, whereas the quantitives of total tannins got 0.054% and 0.037%, respectively. As the effects in Cosmetics by DPPH-method, the antioxidative activities were very strong that the inhibitory ratio showed 75% in leaves and 64% in stems compared with 52% in greentea extract. These are more effective than other plant extracts. The increasing ratio of collagen synthesis rate on the activating fibroblast for extracts of Taxus cuspidata Sieb showed 54.16% (stems) and 33.18% (leaves), To improve the skin elasticity, PPE(porcine pancreatic elastase)-inhibitory activities were strongly effective as 13,7% (stems), 23.5% (leaves) and 66%(seed). Anti-inflammatory acitvity of seed oil was very the above 41% stronger than SG was 24% of anti-Inflammatory as a control sample.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.2
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• pp.119-127
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• 2011

Objective of this study is to identify properties on strength increase of hardened concrete and fluidization of non-hardened concrete using waste ceramics generated by construction waste, which is a type of industrial waste, and by ceramics, which is a clay plastic, during its production process, and determine length change ratio caused by drying shrinkage during substitution of recycle aggregate and waste ceramics, and whether they can be used as concrete compounds. Slump of non-hardened concrete exhibited the best fluidization and formability at recycled aggregate's replacement ratio of 60% driven by higher substitution ratio of recycled aggregate and waste ceramics while air content met the KS requirement when substitution ratio of waste ceramics was $4,000cm^2/g$. Compressive strength of hardened concrete exceeded the requirements at early age and standard age and temperature dropped by roughly $6{\sim}10^{\circ}C$ less than the standard at maximum temperature in adiabatic temperature increase, which will hopefully result in stronger durability.

• Journal of the East Asian Society of Dietary Life
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• v.25 no.6
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• pp.1049-1057
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• 2015

This study investigated the physicochemical properties of Dioscorea batatas for improvement of biological activities during a three-step steaming process, steaming $120^{\circ}C$ for 30 min and hot air drying at $60^{\circ}C$ for 8 hrs. Samples were extracted with 70% ethanol and analyzed for free sugars, and organic acids were analyzed by HPLC. The DPPH & ABTS radical scavenging activities, crude saponin, water binding capacity, oil absorption, and ${\alpha}$-glucosidase activity were measured. The major free sugars in all samples were fructose, maltose, and sucrose, and the free sugar contents increased to 157~235% after the three-step steaming process compared to the control. Organic acid contents of samples treated by steaming increased to greater than 55.8 mg/100g. The ABTS radical scavenging activity significantly increased with repeated steaming process, especially after the three-step process. As the time of steaming process increased, DPPH radical scavenging activity increased from 26.99% fresh Dioscorea batatas to 80.46~97.79% after the three-step process. Crude saponin content increased rapidly as steaming time increased, but decreased after the three-step process steaming process. The water-binding capacities of the samples treated by steaming process were higher than that of the control, whereas oil absorption decreased as steaming time increased. From the results, steaming process could be suggested as beneficial for controlling fat intake. Compared to acarbose, a known antidiabetic drug, used as a positive control, ${\alpha}$-glucosidase inhibitory activity of samples treated by two-step steaming was among the samples. The results suggest that Dioscorea batatas treated by steaming process has antioxidative and anti-diabetis activities and can be used as a natural health product.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.5
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• pp.8-15
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• 2002

We optimally designed the VCO(voltage-controlled oscillator) with spiral inductor using the MOSIS HP 0.5${\mu}{\textrm}{m}$ CMOS process. With the developed SPICE model of spiral inductor, the quality factor of spiral inductor was maximized at the operating frequency by varying the layout parameters, e.g., metal width, number of turns, radius, space of the metal lines. For the operation frequency of 2㎓, the inductance of about 3nH, and the MOSIS HP 0.5 CMOS process with the metal thickness of 0.8${\mu}{\textrm}{m}$, oxide thickness of 3${\mu}{\textrm}{m}$, the optimal width of metal lines is about 20${\mu}{\textrm}{m}$ for the maximum Quality factor. With the optimized spiral inductor, the VCO with LC tuning tank was designed, fabricated and measured. The measurements were peformed on-wafer using the HP8593E spectrum analyzer. The oscillation frequency was about 1.610Hz, the frequency variation of 250MHz(15%) with control voltage of 0V - 2V, and the phase noise of -108.4㏈c(@600KHz) from output spectrum.

• Research in Plant Disease
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• v.13 no.3
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• pp.211-215
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• 2007

Since 2003, anthracnose symptoms on Eucalyptus globulus were observed in farmer's field at Jisepo, Ilwoon-myon, Geoje city, Gyeongnam province, Korea. Typical symptoms of dark brown to black spot appeared on the leaves, twigs, and stems. Infected young trees were wilted, blighted and died eventually. The pathogen isolated from the typical symptom formed gray to dark gray colony on potato dextrose agar and showed optimum growth at $30^{\circ}C$. Conidia were single celled, colorless, cylindrical with obtuse ends, and $9{\sim}22{\times}3{\sim}6{\mu}m$ in size. Appressoria were dark brown, ovate to obovate, and $6{\sim}18{\times}4{\sim}10{\mu}m$ in size. Perithecia were black and globose in shape and $76{\times}274{\mu}m$ in size. Asci were clavate to cylindrical in shape and $42{\sim}76{\times}8{\sim}12{\mu}m$ in size. Ascospores were cylindrical, fusiform, slightly curved at the center, and $10{\sim}23{\times}4{\sim}6{\mu}m$ in size. On the basis of mycological characteristics and pathogenicity test on E. globulus, the pathogen was identified as Glomerella cingulata. This is the first report of the anthracnose on E, globulus caused by G. cingulata in Korea.

• Journal of Mushroom
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• v.10 no.1
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• pp.9-14
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• 2012

This study was carried out to investigated optimum mixing ratio of Korean natural Lindera glauca for production of functional oyster mushroom. Total nitrogen and carbon source of Lindera glauca was 0.16% and 40.9%, respectively and C/N ratio was 215. Total nitrogen source and pH of substrate mixed with Lindera glauca was 2.8~3.0 and 4.8~5.0, respectively. The contents of $P_2O_5$, CaO, MgO and $Na_2O$ were increased by increasing Lindera glauca, but there was no significant difference in $K_2O$ content. Mycerial growth was faster at Lindera glauca treatments than that of control. Yields of fruiting body was the highest at Lindera glauca 20%, and dimeter and thick of pileus were increased according to increase of Lindera glauca addition ratio. The L value of pileus was the highest at the Lindera glauca 10% during mushroom harvest, but there was no significant difference in the a-value and the b-value.