• 한국구조물진단유지관리공학회 논문집
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• 제25권5호
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• pp.182-189
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• 2021

VPS는 중공재의 형상을 최적화 하고 중공재의 부상 및 작업하에 의한 이탈을 방지하는 기능을 가진 기존 중공슬래브공법이다. 본 연구에서는 기존연구에서 제안한 거푸집 패널이 부착된 중공슬래브(VPS)를 활용하여 플랫 플레이트의 뚫림전단 안전성을 검토하였다. 실험 결과, 가력점으로 부터 기둥 폭의 2.0배를 넘어 중공재를 배치한 VSPS 실험체는 기준 실험체에 대비 내력이 9.4%감소하였다. KBC2016에서 제시한 설계 값의 약 1.57배 이상의 강도 값을 나타내었다. 중공재를 배치한 VSPS 실험체가 전단파괴가 발생하기 전까지 기준 실험체 대비 강성의 변화는 없는 것으로 나타났다. 이는 휨 철근을 충분하게 배근하여 본 실험이 전단에 의해 파괴되고 있음을 알 수 있다.

• Molecules and Cells
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• 제42권1호
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• pp.56-66
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• 2019

Histidine triad nucleotide-binding protein (HINT) is a member of the histidine triad (HIT) superfamily, which has hydrolase activity owing to a histidine triad motif. The HIT superfamily can be divided to five classes with functions in galactose metabolism, DNA repair, and tumor suppression. HINTs are highly conserved from archaea to humans and function as tumor suppressors, translation regulators, and neuropathy inhibitors. Although the structures of HINT proteins from various species have been reported, limited structural information is available for fungal species. Here, to elucidate the structural features and functional diversity of HINTs, we determined the crystal structure of HINT from the pathogenic fungus Candida albicans (CaHINT) in complex with zinc ions at a resolution of $2.5{\AA}$. Based on structural comparisons, the monomer of CaHINT overlaid best with HINT protein from the protozoal species Leishmania major. Additionally, structural comparisons with human HINT revealed an additional helix at the C-terminus of CaHINT. Interestingly, the extended C-terminal helix interacted with the N-terminal loop (${\alpha}1-{\beta}1$) and with the ${\alpha}3$ helix, which appeared to stabilize the dimerization of CaHINT. In the C-terminal region, structural and sequence comparisons showed strong relationships among 19 diverse species from archea to humans, suggesting early separation in the course of evolution. Further studies are required to address the functional significance of variations in the C-terminal region. This structural analysis of CaHINT provided important insights into the molecular aspects of evolution within the HIT superfamily.

• 공업화학
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• 제30권2호
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• pp.190-197
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• 2019

활성탄을 사용하여 수용액으로부터 메틸 그린 염료의 흡착에 대해 초기농도와 접촉시간 및 온도를 흡착변수로 사용하여 조사하였다. 흡착평형관계는 Freundlich 등온식에 잘 맞았다. 평가된 Freundlich 분리계수(1/n = 0.212~0.305)로부터 이 흡착공정이 효과적인 처리영역(0 < $R_L$ < 1)에 속하는 것을 알았다. BET식으로부터 얻은 등온포화용량은 온도가 증가할수록 커졌다. Dubinin-Radushkevich식으로 구한 흡착에너지값(E = 316.869~340.049 J/mol)으로부터 흡착공정이 물리흡착공정임을 알았다. 흡착속도실험결과는 유사 2차 반응속도식에 잘 맞는 것으로 나타났다. 자유에너지(-5.421~-7.889 kJ/mol)와 엔탈피(31.915 kJ/mol)는 흡착공정이 자발적이고 흡열반응으로 진행되었다고 알려주었다. 등량흡착열은 평형흡착량이 증가함에 따라 커졌으며, 표면 덮임이 증가됨에 따라 흡착제-흡착질의 총 상호작용도 증가하였다.

• Journal of Ginseng Research
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• 제45권1호
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• 한국항행학회논문지
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• 제22권2호
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• pp.57-63
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• 2018

항공기 발사용 유도무기는 자체점검시험(BIT; built in test) 뿐만 아니라 MIL-STD-1760표준을 준수하여 전원, 이산신호, MUX(multiplexer)통신 등 다양한 시험을 수행해야 시스템 안전성 및 신뢰성이 입증된다. 본 사례 연구 목적은 MIL-STD-1760E 기반 항공기-무장간 연동용 테스트 벤치 구축을 통하여 공대지 유도탄 개발 시 유도탄 전원인가부터 표적정보 입력, 전달정렬, 유도탄 분리 절차에 이르기까지 정의된 TIME LINE에 의거 유도탄 발사 시퀀스 구현 방안을 제시하기 위함이고, 추가적으로 오류삽입기능을 통해 비 활성탄에서 시험할 수 없는 항목까지 검증 범위를 극대화하는 참조 방안이 될 수 있다.

• 한국의상디자인학회지
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• 제24권2호
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• pp.11-26
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• 2022

The Hallyu wave based on K-pop and K-culture has increased the global interest in Korean culture. Therefore, to satisfy consumer demand, there is an attempt to develop a variable fashion design using traditional Korean culture. Transformable fashion design causes changes in form and meaning according to various situations and needs and can induce active participation of consumers. Therefore, we intend to propose a transformable fashion design that is based on traditional Korean culture but appears in a new form and meets the needs of consumers of fashion design. Among Korean traditional culture, wrapping cloths, bojagi are practically used by ordinary people until now, and their shape and use change according to the user's needs. This study intends to derive expression characteristics by examining the expression tendencies of bojagi and to develop transformable fashion designs through the derived data. The purpose of this study is to make and propose transformable fashion designs in real life using the expression characteristics of Korean bojagi. As the method of this study, we first conducted a literature review. Through this, a case study of empirical production was conducted in parallel with the development and production of transformable fashion designs using bojagi. A total of three illustrations and six variable fashion designs were developed. Two of the six works are transformable fashion design that change through movement and expansion of the square shape, the prototype of the bojagi, and two works are modular, bringing changes in function through combination and separation. The work was produced as a multi-purpose transformable fashion design that can be used as clothes or a bag.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제28권3호
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• pp.7-16
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• 2022

Purpose: This study aims to establish the basic directions of the modular airborne infection isolation(AII) ward. Considering a specific function and purposed use as a modular AII ward, it is a chance to derive an address of current modular technology by overview the limitation and improvement of the existing modular architecture. Methods: In addition to the literature analysis on the configuration system of mobile hospitals, research cases on the operational effectiveness of the domestic and foreign mobile construction systems are analyzed. Results: In order to meet the various and strict space guidelines of the AII ward and a chance to improve limitations of uniformed existing modulars, AII modular the negative pressurized care setting should be minimized a structural restriction for reflecting its system on a architectural plan. For this unique requirements, it could be possible to apply various space boxes called infill box which needs to secure a large-scale space. So, a rahmen structure system could be adaptable for this purpose. A dead space between beams of the rahmen structure is to be used for MEP installation. Partial separation, dismantling, and repair should be possible by separating the MEP and infill box from the structure. The infill box must keep 3.5m width under the current Road Traffic Act. Implications: It is necessary to utilize and develop an improved construction method that can reduce the problems of existing steel modular and PC modular.

• Tribology and Lubricants
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• 제39권5호
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• pp.197-202
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• 2023

Recently, research on experimental and analytical techniques utilizing microfluidic devices has been pursued. For example, lab-on-a-chip devices that integrate micro-devices onto a single chip for processing small sample quantities have gained significant attention. However, during sample preparation, unnecessary gases can be introduced into the internal channels, thus, impeding device flow and compromising specific function efficiency, including that of analysis and separation. Several methods have been proposed to mitigate this issue, however, many involve cumbersome procedures or suffer from complexities owing to intricate structures. Recently, some approaches have been introduced that utilize hydrophobic device structures to remove gases within channels. In such cases, the permeability of gases passing through the structure becomes a crucial performance factor. In this study, a method involving the deposition and sintering of diluted Ag-ink onto a silicon wafer surface is presented. This is followed by unstructured nano-pattern creation using a Metal Assisted Chemical Etching (MACE) process, which yields a nanostructured surface with unstructured pillar shapes. Subsequently, gas permeability in the spaces formed by these surface structures is investigated. This is achieved by experiments conducted to incorporate a pressure chamber and measure gas permeability. Trends are subsequently analyzed by comparing the results with existing theories. Finally, it can be confirmed that the significance of this study primarily lies in its capability to effectively evaluate gas permeability through unstructured pillar-like nanostructures, thus, providing quantitative values for the appropriate driving pressure and expected gas removal time in practical device operation.

• 한국염색가공학회지
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• 제35권4호
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• pp.239-245
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• 2023

Cellulose is an abundant biodegradable material in nature with excellent properties, but due to its poor processability, it has been widely studied for processing through modification. Cellulose acetate propionate (CAP) is a cellulose derivative in which the hydroxyl group of cellulose is replaced by acetyl and propionyl groups. CAP has several advantages, such as excellent solubility, structural stability, light and weather resistance, and good transparency. Porous nanofibers with excellent specific surface area, which can be applied in various fields, can be easily formed by the phase separation method using highly volatile solvents. High speed centrifugal spinning is a nano/micro fiber preparation method with advantages such as fast spinning and easy alignment control. In this study, a CAP/polybutylene succinate (PBS) spinning solution with chloroform as solvent was prepared to prepare porous microfibers and the fiber morphology was examined as a function of the disk rotation speed in an high speed centrifugal spinning device.

• 한국동물생명공학회지
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• 제39권1호
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.