• Journal of Mushroom
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• v.7 no.1
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• pp.9-21
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• 2009

This study was executed in an attempt to investigate a artificial requisites of fruitbody occurrence. Environmental requirements on habitat for fruitbody occurrence of collected cultures resulted in leading to $13-16^{\circ}C$ and 75% relative humidity, and requiring silt loam of soil texture which had more nutritional substances than a dry field. Optimal temperature was $25^{\circ}C$, medium PDB, and pH 5.0 in cultural conditions. Mannose required of 5% in ASI 59002, 59003, 59004, but 3% in ASI 59001 was selected as optimum carbon source. The substrates stimulating sclerotium formation were cotton waste, or cotton waste + oak sawdust (mixture ratio of 8:2), which had 20% additive of wheat barn respectively. Sclerotium was formed well in the substrate adjusted chemical properties by applying 2% of calcium sulfate. Sclerotium formation was the most effective in the treatment of peat moss + oak sawdust (mixture ratio of 5:5) + 30% of wheat barn.

• Journal of Mushroom
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• v.5 no.2
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• pp.51-58
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• 2007

These experiment was conducted to find the superior strain selection, cultivation technique and optimum environmental condition of nameko mushroom culture using plastic container. The results was following as Mycelium of Pholiota nameko grown well at MCM and Hamada media, and its media acidity was pH 6~7. The optimum temperature condition for growing mycelium was $25^{\circ}C$. Under $15^{\circ}C$ and above $30^{\circ}C$ of temperature condition, mycelium growing speed was delayed remarkably. Among the 29 strains of nameko mushroom, the most productive strains was JNM19007, JNM19026, JNM19027 and JNM19028. The optimum media composition rate for produce fruitbody was pine sawdust 80% + wheat bran 20%. In this condition, the average fruitbody amount was 188g per 1,100cc container. The optimum post-culturing period was 50 days and mushroom sprout appeared 7 days after old mycelia removed. The suitable temperature was $12^{\circ}C$ for induce sprout, growing period was $16^{\circ}C$ and the optimum relative humidity was 95% in all culturing periods.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.231-238
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• 2015

Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.

• Journal of Mushroom
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• v.3 no.1
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• pp.31-34
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• 2005

This experiment was carried out to examine on the physiological and cultural characteristics of commercial strain Kunneutari #3 of Pleurotus eryngii. The optimal medium suitable for mycelial growth was YM, and followed by MCM and PDA. Also this strain more faster mycelial growth as 6.1 cm/7days compared with commercial strain P. eryngii #1. The optimal mycelial growth temperature was $25{\sim}30^{\circ}C$. The fruitbody yield was increased 54% with $117{\pm}16g/850m{\ell}$ and the fruitbody shape and qualities of this strain was good. And individual weight was $41{\pm}27g$. Spawn run of P. eryngii #3 in bottle cultivation took 30 days and also it took 21 days from scratching of inoculum to harvest that was shorter 3 days than P. eryngii #1, respectively. Therefore, it is expected that cultivation for P. eryngii #3 strain will improve farmer's income by enhancing efficiency of facilities and shorten 6 days on cultivation period, in addition, getting more growing cycle of P. eryngii.

• The Korean Journal of Mycology
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• v.16 no.2
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• pp.95-100
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• 1988

Isozyme band patterns were investigated by isoeletric focusing Esterase and Leucine amino peptidase of Pleurotus spp. in Korea. Esterase patterns of mycelia and fruitbody were distinquished. However, those of primordia, cap and stem were similar. Interspecies differences of Pleurotus ostreatus, P. cornucopiae and P. florida of Esterase zymogram were found. Species identification by electrophoretic zymogram may be a role as an additional taxonomic tool.

• The Korean Journal of Mycology
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• v.28 no.2
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• pp.109-111
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• 2000

Composition of fatty acids (FAs) in Pleurotus ostreatus were analyzed by gas chromatography and compositional changes according to cultivar, fruitbody section and developmental stage (days after primordia formation) were investigated. Major FAs in oyster mushroom were linoleic acid, oleic acid and palmitic acid. Unsaturated FAs were higher in pileus portion than upper- or lower-stipe portion. Oleic acid content was increased with developmental days but linoleic acid content was highest at $3{\sim}4$ days after primordia formation and then decreased after that. And ratio of unsaturated FAs/saturated FAs was decreased with basidiocarp maturation.

• Mycobiology
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• v.36 no.3
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• pp.157-160
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• 2008

This study was carried out to determine the possibility of bottle cultivation utilizing recycled oyster mushroom culture waste as a cultivating substrate for P. ostreatus. Total nitrogen percentage was 0.76%, 1.13%, 1.16%, 1.36%, and 1.38% in the 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; 0.95%, 1.04%, 1.34%, 1.36%, and 1.25% in the 1-, 2-, 3-, 4-, and 5-time postharvest substrate, respectively; and 0.72% and 0.68% in the 2- and 3-time nonadditive substrate, respectively. Weight of the fresh fruiting body harvest was 115 g, 120 g, 117 g, 118 g, and 114 g on 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; and 105 g and 45 g on 2- and 3-time nonadditive substrate, respectively. The first mixed substrate (fresh) and recycled substrates generated no significant difference in the weight of fresh fruiting bodies harvested.

• Journal of Mushroom
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• v.7 no.1
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• pp.22-26
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• 2009

These experiments were conducted to find the optimum mixing rate of used media for saving the production cost of Flammulina velutipes. The materials for media formation was used needle-leaf tree sawdust, media that finished 1th cultivation of F. velutipes, corncob meal, and rice bran, wheat bran as nutrition source. We inoculated the F. velutipes in 14 kinds of different media types and checked the spawn growth speed, fruit body quality and quantity. Two nutrition agents, which is rice bran and wheat bran, did not affected the incubation period, but the effective stem number, quality and quantity of fruit body was better at rice bran than wheat bran. The quality of fruit body produced at mixed 20% of used media (needle-leaf tree sawdust 60% + used media 20% + rice bran 20%) was similar to control plot (needle-leaf tree sawdust 80% + rice bran 20%), but the yield was improved 10% than control plot 130g. According as the used-media mixing amount increases, quality and quantity of fruit body became low remarkably. Therefore, the optimum mixing amount of used-media was 20% and it increased 10% of fruitbody yield.

• Journal of Mushroom
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• v.2 no.4
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• pp.225-227
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• 2004

Hukbaek oyster mushroom were bred and cultivated one after mating spores from Aenutari 1-ho irradiated to X-ray and Byungnutari 1-ho. The major charateristics of the mushroom are showing that pileus is black-brown and stems are pure-white and straight. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $14{\sim}16^{\circ}C$. Around 20 days at $20^{\circ}C$ were required for incubation time and the yield was shown high by 142.8g/bottle. When the cultivating temperature is over $17^{\circ}C$ the color of pileus becomes gray-brown.

• Journal of Mushroom
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• v.2 no.4
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• pp.218-221
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• 2004

Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $15{\pm}1^{\circ}C$. Around 22 days at $20^{\circ}C$ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.