• Journal of Species Research
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• v.8 no.2
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• pp.161-175
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• 2019

During a comprehensive investigation of indigenous prokaryotic species in Korea, a total of 46 bacterial strains assigned to the classes Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, and Epsilonproteobacteria were isolated from a diversity of habitats including freshwater, seawater, brackish water, ginseng soil, plant roots, natural caves, and tidal flats. Based on their high 16S rRNA gene sequence similarities (>98.7%) and formation of strongly-supported phylogenetic clades with the closest type species, each strain was assigned to an independent, predefined bacterial species. Since there were no published or official reports regarding the isolation of these 46 species in Korea, here we report them as new species to Korea: 34 species in 14 families in the five orders of Alphaproteobacteria, 10 species in five families in the three orders of Betaproteobacteria, one species of Deltaproteobacteria and one species of Epsilonproteobacteria. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are described in the species description section.

• The Korea Journal of Herbology
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• v.36 no.5
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• pp.47-57
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• 2021

Objective : For the purpose of developing Convoivulaceae plants recorded in Korean Herbology in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : Books and research papers about Herbology which published at Korea and other countries were examined. Results : A list was made about Convoivulaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Convoivulaceae plants. Convoivulaceae(grew in Korea) were divided into 6 classes with 18 species. Out of those, 6 classes with 13 species were found serviceable which indicates approximately 72% of all. Out of all 18 species of Convoivulaceae family, Calystegia genus were found 6 species, which were shown the most. Among 4 genus of Calystegia species, medicinal plants were all one species each. Out of all serviceable parts in Convoivulaceae, Herb·Radix·Tuber·Semen·Flower·Stem and Folium parts took first place as 8 species. There are 2 species toxic, the survey said. 6 Classifying medicinal plants among the Convoivulaceae plants by part, whole Herbs are found to be 8 separate species each. When it comes to the nature of taste, 10 kinds of taste without action and 7 kinds of sweet taste are the most common. For channel tropism, kidney channel has the largest number of 7 types. Conclusion : A total of 6 genera and 18 species of Convoivulaceae was found in the Korean among which 6 genera and 13 species(approximately 72%) were medicinal plants. Two species toxic drugs were detected.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.85-93
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• 2013

Objective : For the purpose of developing Korean herbalogy of the plants to Iridaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : The examined herbalogical books and research paper which published at home and abroad. Results : A list was made about Iridacease plant which are cultivated or grew naturally in Korea, after investigate the data on domestic and foreign Iridaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Iridacease's properties flavor, channels they use and effects were also noted, not to mention their toxicity. Iridacease (grew in Korea) was divided into 6 classes with 32 species. Out of those, 5 classes with 11 species were found serviceable which indicates 34% of all. Out of all 32 specified Iridacease plants, Iris plants were found 27 species, which were shown the most. And 7 classes of Iris plants were also selected the most in serviceable Iridacease. Out of all serviceable parts in Iridaceae, root parts took first place as 6 species. Conclusion ; There were totaled to 6 genera and 32 species in Iridaceae in Korea and among them medicinal plants are 5 genera, 11 species, some 34% in total.

• The Korea Journal of Herbology
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• v.28 no.4
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• pp.41-48
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• 2013

Objectives : For the purpose of developing Korean herbalogy of the plants to Iridaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : The examined herbological books and research paper which published at home and abroad. Results : A list was made about Eriaceae plant which are cultivated or grew naturally in Korea, after investigate the data on domestic and foreign Eriaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Eriaceae's properties flavor, channels they use, effects, and toxicity were also noted. Eriaceae (grew in Korea) was divided into 9 classes with 48 species. Out of those, 3 classes with 14 species were found serviceable which indicates 29% of all. Out of all 48 specified Eriaceae plants, Rhododendron plants were found 26 species, which were shown the most. And 7 classes of Rhododendron plants were also selected the most in serviceable Eriaceae. Out of all serviceable parts in Eriaceae, leaf parts took first place as 12 species. Conclusion : There were totaled to 9 genera and 48 species in Iridaceae in Korea and among them medicinal plants are 3 genera, 14 species, some 29% in total.

• The Korea Journal of Herbology
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• v.34 no.1
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• pp.81-90
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• 2019

Objective : For the purpose of developing Verbenaceae plants recorded in Korean Herbology in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : Books and research papers about Herbology which published at Korea and other countries were examined. Results : A list was made about Verbenaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Verbenaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Verbenaceae's properties flavor, channels they use, effects, and toxicity were also noted. Verbenaceae(grew in Korea) were divided into 5 classes with 21 species. Out of those, 5 classes with 8 species were found serviceable which indicates 40% of all. Out of all 20 species of Verbenaceae family, Callicarpa genus were found 10 species, which were shown the most. Among 3 genus of Vitex species, medicinal plants were all one species each. Out of all serviceable parts in Verbenaceae, Folium parts took first place as 5 species. There are no toxic, the survey said. Conclusion : There were totaled to 5 genera and 21 species in Verbenaceae in Korea and among them medicinal plants are 5 genera, 8 species, some 38% in total.

• Korean Journal of Heritage: History & Science
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• v.42 no.1
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• pp.100-121
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• 2009

This study is subjected to those gardens of the Korean Folk Village 'Oeam-Ri' designed in 1920s. - transitional period of traditional gardens - and define socio-cultural change's influences and through documents on garden design, descendant's testimony and measured drawings, to understand that period's garden culture's characteristics according to garden design elements. This study applied following analysis methods and procedures to derive out characteristics of transitional garden culture. Analysis on socio-cultural characteristics in 1920s. Analysis on actual condition of transitional garden's design. In this point Outline of the Garden, Space formation, Garden designing elements are (1) water landscape, (2) plant, (3) structures, (4) paving, to derive out characteristics of the transitional garden. The results follow as below; First, during the transitional period 1920s, the economical development, fueled by opening nation's door to foreign countries and indication of collapse of statue systems together with idea of practical science and Enlightenment Thought, was element of changes in garden style. Second, Garden Designers of transitional gardens in 'Oeam-Ri' were limited to upper class of the society. They were wealthy enough to maintain their high social statue in rapidly changing society. As results, tendency of returning to nature developed gardens located in a site of scenic beauty and development of geographical features arranging techniques, and also showed copying foreign styles. Third, arrangement of garden and space composition, in most cases, composed of buildings and yards. Changes in water landscape features and garden spaces are centered to main-yard. Major changes of the garden spaces are water landscapes and plants that showing foreign influences. Fifth, scenic appearance techniques appears with dense garden space and emphasizing visual scenic view. Sixth, the characteristics of transitional garden design techniques are development of geographical feature arranging techniques, changes and mixture of the materials and garden types, emphasizing garden's decorative beauty, change of concept of yard within house into garden, changes from 'borrowing of landscapes' to 'selecting landscapes', changes of front garden from emptiness to fullness, changes of attitudes of enjoying gardens from 'staying calm in the garden' to 'moving or walking in the garden', changes to inner-oriented view, and changes from 'just watching and enjoying the nature' to 'enjoying specific objects'. This study is one of the efforts to restore the identity of Korean Traditional Garden by approaching and observing modern era which function as bridge between tradition and present day, and we observed transitional aspects of changes of traditional garden into modern garden. Hereafter, more studies will be needed to Modern Garden Design be recognized as part of Korean Garden Design History and these would be author's next assignment.

• Journal of the Korean Society for Marine Environment & Energy
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• v.20 no.1
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• pp.26-36
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• 2017

Korea is preparing an offshore carbon capture, transport and storage (CCS) demonstration project which is recognized as one of important $CO_2$ reduction technologies to mitigate climate change. The offshore CCS project aims to transport, inject and store large amount of $CO_2$ into offshore geologic formation, and has a potential risk of leakage which might cause disastrous damage to human health, environment and property. Therefore, in order to ensure the safety of the offshore CCS project, a strict HSE (health, safety and environment) management plan and its implementation are required throughout the project life cycle. However, there are no HSE domestic laws or regulations applicable to CCS projects, and the related research is insufficient in Korea. For the derivation of the essential and urgent requirement in HSE management framework applicable to the offshore CCS project in Korea, we analysed the HSE management methodologies and foreign CCS HSE management guidelines and cases. First, this paper has analyzed ISO 31000, a generalized risk management principles. Second, we have investigated the HSE management practices of CCS projects in Norway and UK. Based on the analyses, we suggested the necessity of developing the HSE Philosophy and the HSE management process through the whole life cycle. Application of HSE management in early phase of an offshore CCS project will promote systematic and successful project implementation in a cost-effective and safe way.

• Korean Journal of Plant Taxonomy
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• v.52 no.1
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• pp.54-63
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• 2022

Prunus subg. Cerasus is the most planted street and landscaping tree in South Korea, but it is difficult to identify species according to their macro-morphologies, leading to problems when attempting properly to manage species quantities. The purpose of this study is to understand the current status of plant types and species compositions in Bundang Central Park in Bundang-gu of Seongnam City and to discuss the necessity of the management of landscaping tree planting. In April of 2021, during the cherry blossom season, a total of 5,866 planted cherry trees were investigated within an area of 6 km² of Bundang-gu in Seongnam City. As a result, 5,744 trees were sorted into eleven taxa, but the remaining 122 trees were not determined due to their complex morphologies. Prunus ×yedoensis Matsum. accounted for the highest proportion (52.1%), followed by P. serrulata Lindl. var. pubescens Nakai and P. jamasakura Siebold ex Koidz. P. ×nudiflora (Koehne) Koidz., a plant native to Jejudo Island, was not found in this survey. In order to help identify cherry trees based on micro-morphologies, an identification key was presented for the eleven taxa planted as major landscaping trees. It is known that cherry trees frequently form interspecific hybrids in nature. In order to prevent a loss of the genetic originality of native species due to hybridization and gene introgression from foreign cherry trees, it is necessary to manage planting species near the habitats of native taxa and track their origins.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.420-432
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• 2011

Population dynamics of greenhouse whitefly, Trialeurodes vaporariorum (Westwood), were modeled and simulated to compare the temperature effects of air and tomato leaf inside greenhouse using DYMEX model simulator (pre-programed module based simulation program developed by CSIRO, Australia). The DYMEX model simulator consisted of temperature dependent development and oviposition modules. The normalized cumulative frequency distributions of the developmental period for immature and oviposition frequency rate and survival rate for adult of greenhouse whitefly were fitted to two-parameter Weibull function. Leaf temperature on reversed side of cherry tomato leafs (Lycopersicon esculentum cv. Koko) was monitored according to three tomato plant positions (top, > 1.6 m above the ground level; middle, 0.9 - 1.2 m; bottom, 0.3 - 0.5 m) using an infrared temperature gun. Air temperature was monitored at same three positions using a Hobo self-contained temperature logger. The leaf temperatures from three plant positions were described as a function of the air temperatures with 3-parameter exponential and sigmoidal models. Data sets of observed air temperature and predicted leaf temperatures were prepared, and incorporated into the DYMEX simulator to compare the effects of air and leaf temperature on population dynamics of greenhouse whitefly. The number of greenhouse whitefly immatures was counted by visual inspection in three tomato plant positions to verify the performance of DYMEX simulation in cherry tomato greenhouse where air and leaf temperatures were monitored. The egg stage of greenhouse whitefly was not counted due to its small size. A significant positive correlation between the observed and the predicted numbers of immature and adults were found when the leaf temperatures were incorporated into DYMEX simulation, but no significant correlation was observed with the air temperatures. This study demonstrated that the population dynamics of greenhouse whitefly was affected greatly by the leaf temperatures, rather than air temperatures, and thus the leaf surface temperature should be considered for management of greenhouse whitefly in cherry tomato grown in greenhouses.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.87-94
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• 2008

Porcine epidemic diarrhea virus (PEDV) is an infectious and highly contagious virus of swine. In order to develop the transgenic tobacco culture cells producing PEDV antigen protein, four vectors expressing PEDV spike protein (SP) gene under the control of a CaMV 35S promoter were constructed. Four fragments of the SP region of PEDV, SP1 (444 bp, 1487-1930 bp), SP2 (1.7 kb, 2300-3987 bp), SP3 (1.4 kb, 1559-2950 bp), and SP4 (2.6 kb, 9-2643 bp) were amplified by PCR and then C-MYC tag was fused to the end of each SP gene, respectively. These cassettes are inserted into the pCAMBIA2300 (named as 35S::SP1-M, 35S::SP2-M 35S::SP3-M, and 35S::SP4-M, respectively). Tobacco (cv. BY-2) cultured cells were transformed by co-cultivation with Agrobacterium tumefaciens harboring expression vector. We selected kanamycin-resistant calli and checked for the presence of the introduced SP gene using PCR, resulting 70% of them showed the foreign gene. We selected the lines with high-level expression of PEDV antigen protein based on dot blot analysis. Southern blot analysis confirmed that the PEDV SP gene was integrated into the genome of the tobacco cultured cells. Northern blot analysis showed that the introduced gene was highly expressed in transgenic cultured cells. Transgenic tobacco cultured cells-derived antigen induced immunogenicity in mice as determined by a plaque reduction neutralization assay. These results suggest that the vectors expressing PEDV spike protein gene in this study will be useful for the development of transgenic plants and cultured cells producing PEDV antigene protein.