• 한국초지조사료학회지
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• 제17권3호
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• pp.285-292
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• 1997

This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x $10^5$ pfu/m${\ell}$, therefore the library might cover all expressed mRNAs. Using pho5 as a probe. tobacco Apase cDNA was cloned, and restriction mapping and Southern blot analysis of cDNA insert were revealed that the 3.6 kb cDNA contained tobacco acid phosphatase cDNA. Plasmid pGA695 -tcAPl was constructed by subcloning tobacco Apase cDNA into the Hind site of pGA695 with 35s promoter which can be expressed constitutively in plants. The Brassica napus cotyledonary petioles were cocultivated with the ,4 grobacteriunz and transferred to the selection medium. The transformed and regenerated plants were transplanted to soil medium. Southern blot analysis was done on the transformed plants, and it was confirmed that a foregin gene was stably integrated into the genonies of B. nnpus plants.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.125-129
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• 1994

A field survey was carried out in Korea to assess the mineral composition of rice straw since it is a cheap and available cattle feedstuff. Forage and soil samples were collected in 4 localities in the Kyongbuk province. Soil analysis were also carried out in order to establish the relationships between soil composition and mineral content in the rice straw. Based on NRC tables, the rice straw samples provided adequate amounts of Mg, Ca, K, S, Mo, Mn and Zn. Percentages of samples deficient in P, Na, Cu and Se were 83, 50, 67 and 83 respectively. Soil samples, whose mean pH was 5.8 and mean organic matter content was 3.99%, were comparatively acidic and had high organic matter content. They also had high amounts of extractable Fe and Mn. Correlation coefficients between mineral content in soil and rice straw were low, i.e., 0.42 for Me (p < 0.05), and 0.37 for Mo (p < 0.05). The low or nonexistent correlation between soil and forage composition indicates the difficulty of establishing appropriate methods of mineral availability to the plants.

• 한국초지조사료학회지
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• 제13권2호
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• pp.132-138
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• 1993

Yield and plant constituent responses of forage sorghum cultivars have usually been compared in uniform defoliation management test. However the influence of harvest time on differential response of cultivars needs more precise definition. Therefore this study with sorghum-sorgo-sudan hybrid cv. NC+Sweet Leaf, and Super Su 22 and Sorghum-Sorghum hybrid cv. Pioneer 931 was carried out under two defoliation regimes, namely defoliation at heading stage of each variety and defoliation on the same calendar date in response to heading stage of early variety. The results are summarized as follows; 1. Three harvests were taken by early variety with 80 days and two harvests by late variety with 94 days from sowing to heading. 2. Dry matter and TDN yield tend to be higher when the plants are cut at ear emergence stage of late variety. 3. Crude protein content was similar for the same growth growth stage of 1st growth and 1st regrowth, and rather big different between varieties. 4. Considering only dry matter and TDN yield, it is recommendable to cut two times at ear emergence stage of late variety and also three times at ear emergence stage of early variety in view point of utilization period extension and distribution of forage products.

• 한국자원식물학회지
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• 제7권1호
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• pp.53-61
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• 1994

This study was carried out in Mt. Backdu which is located at the borderland between Korea and China, to offer the information and help the people who in order to study and utilize the northernresource plants in future. Actually, the exploration and investigation were executed only in china sideof Mt. Backdu and its outskirts.Totally, 100 families, 281 genus and 703 species were investigated. They were classified into 26 spe-cies of pteridophyta, 14 species of gymnospermae, 101 species of monocotyledons and 562 species of di-cotyledons by general taxonomic classification, and 594 medicinal plants(84.5% ), 296 ornamentalplants(38.3% ), 161 edible plants(22.9% ), 92 industrial plants, 67 honey plants and 17 forage plantsby use.

• 한국작물학회지
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• 제40권3호
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• pp.351-358
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• 1995

1. 노천이장한 경우 발아가 양호하여 단위면적당 입모수가 많았는데, 1992년산 무처리구의 187개/m$^2$에 비하여처리종자는 292개/m$^2$였으며, 1991년산 처리종자구는 78개/m$^2$로 입모가 불량하였다. 2. 생육초기의 생초생산량은 단위면적당 개체수와의 관계가 높았으나 생육이 진전됨에 따라서 개체수의 효과는 저하되는 경향이었다. 3. 1차 예취시의 예취높이를 5cm로 하였을 때는 10cm에 비하여 생초수량이 높았으나 2차 예취시의 수량은 떨어졌다. 그러나 총수량은 예취 높이간에 유의한 차이가 없었다. 4. 미국개기장은 번종후 40~50일부터 예취를 시작하여 1개월 간격으로 3~4회 예취가 가능하였으며, 10a당 생초수량은 9~10톤, 건초수량은 1.4~1.8톤 이었다. 5. 예취시기에 따른 미국개기장의 생초와 건초수량은 생육기간 및 생육기간의 적산온도와 유의한 상관관계가 있었다.(r =0.711~0.860). 6. 파종기가 빠르고 예취시기가 이를수록 엽신의 비율이 높았다. 7. 미국개기장은 조단백질 16.32%, 조지방 24.01%, 조회분 11.59%를 함유하고 있어서 다른 화본료 사료작물에 비하여 품질이 좋은 편이었다.

• 한국초지조사료학회지
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• 제16권3호
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• pp.183-189
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• 1996

The productivity and utilization of four different grasdlegume pastures for grazing management were examined. The pasture mixtures used in this study were orchardgrass + ladino clover (Tl), orchardgrass + alfalfa (T2), perennial ryegrass + alfalfa (T3), and orchardgrass (T4, without legumes) respectively. The pastures were mtationally grazed 7 times with growing cow which had an initial weight of ca 243kg. The gmwth of pasture plants, dry matter (DM) yield, DM distribution, daily DM production, and pasture utilization were investigated at the NLRI, in Suwon, from April to October in 1991 and 1992. The average plant height at each grazing was 32-33m. The average DM content was 17.8%, wil the lowest in TI (16.4%) and highest in T3 and T4 (18.4%). The annual DM production were 9,609 kg (T3), 9,420 kg (Tl), 9,402 kg (T2), and 9,194 kgha (T4) in that order, but no significant differences were found in forage yield between treatments. Most of forage DM was produced in spring season, particularly in May. The daily DM production was highest in May (81.9kg), and lowest in August (33.0kg/ha). The average efficiency of pasture utilization was about 79%, with the lowest in TI (77%), and highest level of utilization was observed in T4 (82%). In this experiment, production and utilization of each pastures were observed similarly. However, the forage nutritive value, botanical composition, and animal performance as affected by different pasture mixtures for suitable grazing management are needed to ascertain.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.425-441
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• 2010

Forages are the backbone of sustainable agriculture. They includes a wide variety of plant species ranging from grasses, such as tall fescue and bermudagrass, to herbaceous legumes, such as alfalfa and white clover. Abiotic stresses, especially salinity, drought, temperature extremes, high photon irradiance, and levels of inorganic solutes, are the limiting factors in the growth and productivity of major cultivated forage crops. Given the great complexity of forage species and the associated difficulties encountered in traditional breeding methods, the potential from molecular breeding in improving forage crops has been recognized. Plant engineering strategies for abiotic stress tolerance largely rely on the gene expression for enzymes involved in pathways leading to the synthesis of functional and structural metabolites, proteins that confer stress tolerance, or proteins in signaling and regulatory pathways. Genetic engineering allows researchers to control timing, tissue-specificity, and expression level for optimal function of the introduced genes. Thus, the use of either a constitutive or stress-inducible promoter may be useful in certain cases. In this review, we summarize the recent progress made towards the development of transgenic forage plants with improved tolerance to abiotic stresses.

• 한국초지조사료학회지
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• 제41권2호
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• pp.84-95
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• 2021

The purpose of this study was to assess the forage productivity and nutritive value of kenaf at different fertilizer application amounts and various stages of maturity. The experiment was conducted from May to September 2020, the amount of 80 kg of kenaf seed/ha was supplied with different types and amounts of nitrogen fertilizer and the plants were harvested at 10-day intervals from different harvesting dates (24^th August and 3^rd, 13^th, 23^rd September). According to the different fertilizer types and application amounts, the highest kenaf height was recorded in the inorganic fertilizer amounts of 200 and 250 kg N/ha and the fresh and DM yield were significantly improved in the inorganic nitrogen amount of 250 kg N/ha. The highest CP and TDN content in the leaf was achieved in the inorganic fertilizer amounts of 150 and 200 kg N/ha, respectively; and the highest TDN content in the stem was also found in the inorganic fertilizer amount of 200 kg N/ha. According to the different harvesting dates, the highest DM ratio was found in the harvesting date of 13th September, the leaf ratio increased with advanced maturity, whereas the stem ratio decreased significantly and the highest DM yield of kenaf was recorded in the harvesting dates of 13^th and 23^rd September. Besides, the highest CP, CF, CA, ADF, NDF and TDN content in the leaf as influenced by different harvesting dates was 15.4, 31.8, 10.2, 22.1, 34.7 and 76.5%, respectively, and the CP, CA, ADF and TDN in stem decreased significantly with advanced maturity of kenaf. In conclusion, the optimal fertilizer amounts and the appropriate harvesting dates for a high forage yield and high-quality kenaf as livestock feed were the inorganic fertilizer application amounts of 200-250 kg N/ha and from 13^th and 23^rd September, respectively.

• 한국초지조사료학회지
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• 제18권3호
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• pp.259-266
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• 1998

A field experiment was carried out to determine the effects of mechanical and chemical drying agents at mowing on the field drying rate and hay quality of orchardgrass dominant pasture plants. The mower conditioner /chemical drying agent ($K_2CO_3$ 2% conditioning, $K_2CO_3$ 2% + conditioning and control) were treated at different harvest stages (late boot, heading and bloom stage) for hastening hay manufacture in 1996. After field dry, four square bales were made by hay baler, and the visual estimation and nutritive value of hay were evaluated after storing two months. The field drying rate of pasture plants was higher with delayed stage of harvest, and mechanical and mechanical + chemical, but chemical alone was very low. In mower conditioning, the duration of field dry was shortened by 0.5 to 1 day compared with $K_2CO_3$ and 1.5 to 2 days compared with control. The dry matter loss of hay was reduced by late harvest and mechanical, and mechanical + chemical, but the score by chemical alone was very low. The visual score (leafiness, green color, odor and softness) of hay after storage was high in mechanical and mechanical + chemical, but the score by chemical alone was very low. Nutritive value (ADF, NDF, digestibility, and relative feed value) of hay was also high with treatment of mechanical and mechanical + chemical, but the quality by chemical alone was similar compared with control. The quality of hay was very low when harvested at bloom stage. In conclusion, mower conditioning can enhance the field drying rate of orchardgrass dominant pasture plants, however the drying efficiency of chemical drying agent was very low. Harvesting at early heading to heading stage was recommened for manufacture of high quality hay.

• 한국초지조사료학회지
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• 제26권2호
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• pp.83-90
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• 2006

환경스트레스에 내성을 갖는 버즈풋 트레포일(Lotus corniculatus L.) 형질전환체를 개발하기 위하여, AtNDPK 유전자가 E35S 프로모터에 의해 조절되도록 재조합한 발현벡터 pEN-K를 Agrobacterium 형질전환 방법으로 버즈풋 트레포일에 도입하였다. Agrobacterium과 공동배양한 버즈풋 트레포일 캘러스를 $100{\mu}g/m1$의 kanamycin 및 $500{\mu}g/m1$의 cefotaxim을 첨가한 SH-3-kc 배지에서 배양하며 식물체로 재분화시켰다. 재분화된 버즈풋 트레포일의 genomic DNA를 분리, PCR 및 Southern blot 분석을 실시한 결과, AtNDPK 유전자가 도입된 형질전환체의 경우 agarose gel 전기영동 및 X-ray 필름상에서 DNA band 및 hybridization signal을 확인할 수 있었으나, 형질전환되지 않은 대조구의 버즈풋 트레포일에서는 DNA band 및 hybridization signal이 관찰되지 않았다.