• Journal of Plant Biotechnology
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• 제37권4호
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• pp.425-441
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• 2010

Forages are the backbone of sustainable agriculture. They includes a wide variety of plant species ranging from grasses, such as tall fescue and bermudagrass, to herbaceous legumes, such as alfalfa and white clover. Abiotic stresses, especially salinity, drought, temperature extremes, high photon irradiance, and levels of inorganic solutes, are the limiting factors in the growth and productivity of major cultivated forage crops. Given the great complexity of forage species and the associated difficulties encountered in traditional breeding methods, the potential from molecular breeding in improving forage crops has been recognized. Plant engineering strategies for abiotic stress tolerance largely rely on the gene expression for enzymes involved in pathways leading to the synthesis of functional and structural metabolites, proteins that confer stress tolerance, or proteins in signaling and regulatory pathways. Genetic engineering allows researchers to control timing, tissue-specificity, and expression level for optimal function of the introduced genes. Thus, the use of either a constitutive or stress-inducible promoter may be useful in certain cases. In this review, we summarize the recent progress made towards the development of transgenic forage plants with improved tolerance to abiotic stresses.

• 한국초지조사료학회지
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• 제24권2호
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• pp.171-176
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• 2004

유기조사료 생산을 위한 방목초지의 역할을 구명하기 위해 2000년부터 2002년까지 경기도 수원시 소재 축산기술연구소에서 수행되었다. 유기재배에 따른 목초의 생산성을 구명하기 위해 대조구(표준관리), 친환경구, 유기구를 처리구로 하여 수행되었다. 그 결과는 다음과 같다. 1. 건물수량은 대조구, 친환경구, 유기구의 순으로 많았으나, 유기구와 대조구의 수량차이가 14% 정도로 작았다. 2. 사료가치와 식생에서는 큰 차이가 없었다. 위 시험의 결과 방목초지는 유기적 재배에서도 양호한 상태로 유지되어 초지는 유기조사료 생산에 적합한 작목으로 판명되었다.

• 한국초지조사료학회지
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• 제39권1호
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• pp.45-52
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• 2019

본 시험은 목장 경영형태에 따른 산지초지 조성시 생산성과 사료가치의 변화를 관찰하기 위하여 수행되었다. 시험에 이용된 초지는 강원도 평창군에 위치한 서울대학교 평창캠퍼스의 시험목장내에 2014년 9월 3일에 조사료 생산형(Forage production type, FP; 오차드그라스 18 + 톨페스큐 12 + 티머시 5 + 화이트클로버 5 kg/ha) 초지와 공공 목장형 (Public farm type, PF; 오차드그라스 12 + 톨페스큐 18 + 티머시 5 + 화이트클로버 5 kg/ha) 초지를 각각 0.5ha씩 조성을 하여 매년 4회 수확 또는 방목을 실시하였으며 파종된 목초의 생육특성, 수량 및 사료가치를 조사하였다. 초장은 화본과목초는 1차 수확시 가장 길었으며 두과목초는 2차 수확시에 가장 길었다. 건물함량은 매년 1차 수확시 가장 높았으며 2차 수확시 유의적으로 낮았다 (p<0.05). 그러나 2년차에서는 2차, 3차 및 4차 수확에 따른 차이는 없었다 (p>0.05). 식생변화에 있어서는 조성이후 시간이 경과함에 따라 두과목초의 비율이 점차 증가되는 경향을 보였으며 2차 및 3차 수확시 잡초와 나지의 비율이 높았지만 4차 수확시는 줄어들었다. 생초 수량은 1년차 전 수확시기에서 목장 형태별 유의성이 있었으나 2년차에서는 유의적인 차이가 없었다. 건물 수량도 생초수량과 같은 경향을 보였으며 전체적으로는 조사료 생산형 초지의 수량이 유의적으로 높게 나타났다 (p<0.05). 공공목장형 초지의 가축 섭취량은 1년차에서는 1,452 kg/ha 이었으나 2년차에는 1,743 kg/ha로 더 높았으며 방목이용율은 3차 방목시 가장 높게 나타났다. 경영형태별 초지의 사료가치는 큰 차이가 없었으나 수확시기 간에는 차이가 나타났다. 조단백질 함량은 1차 수확시 가장 낮았으며 TND 함량은 1차 수확시 가장 높았고 RFV 값은 4차 수확시 가장 높았다. 이상의 결과를 종합하여 볼 때 한국형 산지초지 조성을 위해서는 현장 상황에 맞는 초지의 조성이 중요하며 생산성에 있어서는 조사료 생산형 모델이 더 우수한 것으로 나타났으며 도출된 결과들은 향후 공공목장형 초지 이용을 위한 기초 자료로 제공되었으면 한다.

• 한국초지조사료학회지
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• 제11권3호
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• pp.182-188
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• 1991

With a purpose of finding out the effects of cattle slurry applicatioin on the dry matter yield, yield component, and botanical composition in the mixed sward, a field experiment was conducted with two times and five levels of cattle slurry application. The experimental field was laid.out in a split-plot design with three replications, and lasted from September. 1986 to October, 1990 at the Livestock Experiment Station in Suweon, The results obtained are summarized as follows: 1. At each harvest time, the plant growth of the plots with cattle slurry application was slightly worse when compared to dressing of mineral fertilizer. 2. Average dry matter yield for 4 years was not significantly different between the slurry application times, just after cutting, and 15 days after cutting, The DM yield in the plot with cattle slurry 30 m!/ha and mineral fertilizer(9,095 kg/ha) and with cattle slurry 60 m1/ha and mineral fertilizer(8, 987 kg/ha) were decreased by 8% and 10% than that of the plot with mineral fertilizer application(9,937 kg/ha), respectively. 3. The DM yield component of the plots with cattle slurry application just after cutting was much better when compared to manuring 15 days after cutting. And it was much better in the plot with cattle slurry 60 m"/ha and mineral fertilizer which was composed of 73.8% grasses(6.636 kg/ha). 24.6% legumes (2,207 kdha) and 1.6% weeds(144 kdha). 4. In general, the botanical composition was relatively good in the plots with slurry application just after cutting. Grassland vegetation of the plots with cattle slurry 60 m"/ha and mineral fertilizer was changed into much better botanical composition with 66.8% grasses, 30.2% legumes and 3. 1% weeds at the end of the experiment.xperiment.

• 한국초지조사료학회지
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• 제16권1호
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• pp.61-68
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• 1996

Hehage production, botanical composition and soil chemical characteristics were investigated Ween new pastures and old pastures during grazing seasons from May 1993 to October 1994, to find out the condition of pastures grazed by horses on Chdu. Dry matter yields between new pastures and old pastures were 8,757kglha and 7,486kglha respectively. Plant heights of new pastures were 17.4cm. compared with those of old pastures of 12.lcm. The botanical composition of new pastures was composed of grasses about 402, legumes 7~14%, others 18-30% and dead materials 18~ 25%. whiie there were grasses 12%, legumes 3~7%, others 65% and dead materials 15% in old pastures. The contents of crude protein, phosphoms and sodium were lower in old pastures(l3.30, 0.24 and 0.10% respectively) than those in new pastures(l5.47, 0.28 and 0.14% respectively). There was no difference in ADF, NDF, Ca, Mg and K content between the 2 kinds of pastures. The chemical characteristics of the 2 kinds of pastures was shown to be similar, except available phosphorus, comparing 8.18ppm in old pastures with 84.43ppm in new pastures. Although the herbage yield and the soil characteristics of old pastures were lower than those of new pastures, we suppose that the old pastures would be improved, if taken good care of by methods such as oversowing and sometimes applying fertilizer.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.53-62
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• 2004

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

• 한국초지조사료학회지
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• 제9권1호
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• pp.1-6
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• 1989

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein(CP) content and dry matter(DM) yield of grasses for soiling. Application times of fertilizer were March 10, 20, 30, April 9 and 19, and control (non-fertilized) plot was involved. All fertilized plots were also treated with single- and compound fertilizer. The first harvesting date for soiling was May 12, and the regrowth soiling yield was investigated on June 9, 1988. In all fertilized plots, the growth, DM yield, CP, CP yield and regrowth yield of gasses were significantly increased compared with control, and the best grass growth, DM yield, CP, CP yield and N recovery were observed on March 30 and April 9 among all fertilized plots. And there were not significant differences between singleand compound fertilizer treatment in grass growth, DM yield and CP content. On March 30 and April 9 in this experiment, the accumulated temperature was 120 and 200^{\circ}C.$ and the subsoil temperature at 10 cm depth was 8 and 10^{\circ}C.$. Considering the average meteorological condition in Suwon area, the optimum application time of spring fertilizer for soiling may be recommended from April 3 to April 10.

• 한국초지조사료학회지
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• 제8권3호
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• pp.141-146
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• 1988

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein (CP) content and dry matter (DM) yield of gasses for grazing Application times of fertilizer were on March 10, March 20, March 30, April 9 and April 19, and control (non-fertilized) plot was involved. All fertilized plots were also treated with single and compound fertilizer. The first harvesting date for grazing was on April 30, and the regrowth soiling yield was investigated on June 9, 1988. In all fertilized plots, the growth, DM yield, CP, CP yield and nigrogen recovery of grasses were significantly increased compared with control, especially on the plot of March 30. The regrowth yield on March 30, April 9 and April 19 were shghtly hig!!a than those of the others. And there were not significant differences of grass growth, CP content, and DM yield between single-and compound fertilizer treatment. On March 30 which was the best time of fertilizer application in spring, the accumulated temperature was 116.6^{\circ}C.$ and the subsoil temperature at 10 cm depth was 8.1 - 8.6^{\circ}C.$. Considering the average meteorological condition in Suwon area, the optimum application time of spring fertilizer for grazing may be recommended on March 30-April 3 (accumulated temp.; 100-125^{\circ}C.$, subsoil temp.; 8-9^{\circ}C.$).

• 한국초지조사료학회지
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• 제10권3호
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• pp.152-157
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• 1990

To find out the effect of different patterns of nitrogen and potassium distribution on dry matter yield and botanical composition of temperate pastures, a field experiment was conducted with 6 treatments replicated 3 times in a randomized complete block design. It was lasted from September, 1986 to October, 1989 at the Livestock Experiment Station in Suweon. The results obtained are summarized as follows: 1. In early spring, winter hardiness, growth vigour, cold damage and coverage of grasses with 30% dressing of the total amount of N and K20 fertilizer in spring and at the 4th cutting time respectively were better than those of the other treatments. 2. Average dry matter yield for 3 years with heavy dressing in spring and at the 1st cutting time(l1, 187 kg DM/ha) was much more increased by 9% than that of the equally fertilized treatment(l0,24lkg DM/ha). 3. Changes in the botanical composition showed, in general, the same tendency for all treatments except equal N and K20 distribution. However, grassland vegetation with heavy dressing in spring and at the 1st cutting time was changed into relatively good botanical composition with 76.7% grasses, 22.3% legumes and 1.0% herbs at the end of the experiment. 4. By DM yield and botanical composition treatment 3(40-30-15-0-1570) seemed to be an optimal nitrogen and potassium distribution pattern in a temperate pasture.

• 한국초지조사료학회지
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• 제15권3호
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• pp.198-206
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• 1995

본 연구는 1991년부터 1993년까지 사료작물과 목초류 7종(호맥, 대맥, 연맥, Italian ryegrass, alfalfa, orchardgrass 및 혼과목초)을 공시재료로 하여 곤포사일리지(BS=baled silage making)조제이용을 위한 수확시기와 이때의 BS 생산성 및 품질을 평가하였다. 시험방법에 있어서 작물재배는 농촌진흥청의 사료작물 및 목초 표준경종방법에 준하여 실시하였다. 사일리지 조제작업중 BS는 자체적으로 제작한 각형 베일러를 이용 개체당 크기 90cm$\times$60cm$\times$50cm, 중량은 건물기준 20kg 내외, 곤포압착 21~23mm를 유지하여 조제하였다. 한편 고수분 재료는 수분정도에 따라 예건처리(무처리, 0.5일 및 1.0일)와 formic acid(무처리, 0.3% 및 0.6%)를 병행 처리하여 BS 를 조제하였다. 맨류작물의 BS 생산을 위한 수확적기는 대맥, 황숙기, 호맥과 연맥은 유숙기 이었으며 이때의 BS 수량은 각각 11.92톤(대맥), 12.64톤(호맥) 및 8.41톤/ha(연맥)이었다. Italian ryegrass 및 목초의 수확적기는 각 초종 모두 개화기로서 년간 BS 생산성은 Italian 13.81톤(2회 예취), 혼파목초 11.46톤(3회), alfalfa 10.62톤/ha(3회)이었다. 한편 조기수확에 따른 고수분 재료의 BS 품질은 무처리시의 35점에서 예건 및 FA 동시처리시에는 84점으로 향상되었다. Italian tyegrass의 BS품질도 무처리시의 61점에서 예건 및 FA 처리시에는 88점으로 개선되는 효과가 있었다.