• Journal of Dairy Science and Biotechnology
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• 제41권4호
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• pp.203-210
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• 2023

Bacterial contamination negatively affects the quality, functionality, and safety of dairy products. Adherent populations of bacteria, referred to as biofilms, grow on the surfaces of dairy processing equipment and are the primary cause of dairy contamination. In addition, microorganisms present in the farm environment and dairy factory can contaminate the Clear-In-Place (CIP) line through raw milk transport pipes; therefore, exhaustive management is required. In dairy manufacturing facilities, biofilm formation is controlled using CIP systems that primarily require sodium hydroxide and nitric acid. However, the leakage or incomplete removal of these potently active compounds can be harmful to humans. In the present study, we compared the eradication of Escherichia coli and other bacteria using commercially available combinations of sodium hypochlorite (NaClO) and citric acid, which are recognized by the Korean Ministry of Food and Drug Safety (MFDS) as food disinfectants. When considered in the CIP system of the field manufacturing process, E. coli was not detected (compared to detection before treatment), and other bacteria were detected at 0-32 culture-forming units (CFU)/cm². The residual amount of chlorine ions after CIP treatment was similar to that in tap water, and there was no significant difference in the overall components of the fermented dairy products. Therefore, the NaClO/citric acid CIP system can be safely applied in dairy manufacturing processes.

• 한국학교보건학회지
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• 제16권2호
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• pp.13-23
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• 2003

To provide data necessary for effectively applying the HACCP system by understanding the current application condition of HACCP system and satisfaction level of the dietician in elementary schools, a mail-in survey was conducted on dieticians serving for 227 elementary schools applying HACCP system in Kyungsangbuk-Do since November 1, 2001 to December 20, 2001. 83.5% of the subjected schools were conducting more than 50% of HACCP cooking process management, and the level of cooking process management displayed significant relevance according to the number of dieticians serving the school meals. The area that was not well conducted in the field of HACCP system was proven to be water examination(94.0%), inspection on self-sanitation of cooks prior to cooking(90.6%), and maintenance of dry kitchen floor(l4.8%). The reason why the above areas are not well conducted was because of lack of time due to over workloads. Subjective dieticians had pointed out improvement of sanitary concept(58.1%) and improvement of self-sanitation (28.8%) as benefits of applying HACCP. 21.2% of the subjective dieticians were satisfied with application HACCP and 35.2% were dissatisfied with applying HACCP. In case of which the duration of applying the HACCP was longer than one year and in case of higher rate of HACCP cooking process management and longer work experience of the dieticians, the level of satisfaction was proven to be significantly higher. The most difficult things to follow in important management categories according to the features of dietitian work and work experience were food distribution of CCP7 step and maintenance of optimum temperature(70.7%). Subjective dieticians had pointed out insufficient facility or environment and lack of inspection equipments in order regarding problems of applying HACCP. Also in the level of necessity of improvement categories in applying HACCP, dieticians had replied that facility and equipment improvement was mostly needed. Due to the induction of HACCP system in school meals, comparatively well cooking process management is being conducted, and I believe it could contribute in securing safety and quality improvement of school meal by improving the sanitation concept of the dieticians. However, the satisfaction level of dieticians are rather low and there are many difficulties in maintaining optimum temperature in the process of food distribution and transportation process. Also, lack of facilities and environment, lack of inspection equipments and etc are pointed out as problems of inducing HACCP. Thus, to settle HACCP system, it is believed that brave investment must be preceded.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.862-863
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• 2012

최근 전세계적으로 먹거리에 대한 빈번한 사고로 인해 소비자들은 안전한 농산물의 유통에 대한 관심이 높아지고 있다. 또한 한미 FTA 등의 자유무역체결로 인해 농산물의 국제적 이동이 증가함에 따라 안전한 농산물의 유통은 소비자의 신뢰 및 경쟁력 확보를 위한 기업의 중요한 경쟁요인으로 작용하고 있다. 따라서 농산물의 안전관리를 위해서는 국내외적으로 유입되는 농산물의 생장환경이력과 유통이력 등의 정보를 조회하는 시스템이 필요하다. 본 논문에서는 소비자의 스마트폰을 활용하여 매대에 진열되어 있는 농산물의 이력을 확인할 수 있는 소비자 중심의 농산물 이력관리시스템의 구축 방안을 제안한다.

• 현장농수산연구지
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• 제11권1호
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• pp.63-77
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• 2009

The decrease in the farming rates of agricultural produce importing countries has led to concerns over stable food supply and food safety and this situation demands a change in awareness on agricultural problems arising from the depletion of natural resources and environmental destruction. To preserve agriculture, importing countries are emphasizing the various roles of agriculture in order to revive its multi-functional aspects. It is obvious that differentiated strategies are needed to create values from agricultural production, and a liberal arts education can play an important role in making these strategies. However, training programs for future agricultural CEOs of the Korea National Agricultural College(KNAC) attaches too much importance on production skill and management rather than focusing on a liberal arts education. On the other hand, Poolmoo Ecological Agriculture Course carried out by Poolmoo Agriculture Technical High School located in Hongseong-Gun, Chungnam Province (which puts a high value on ecological fanning) gives more weight on a liberal arts education, taking up more than 50% of the curriculum. Also, the National Farmers Academy in Japan which reformed its whole system in 2008, has started various liberal arts educational programs. The Academy fosters creative agricultural management, attractive rural development enhancing a comfortable rural life and educates on the future of Japanese food and international environment. It is expected that the ecological and cultural roles of farming will grow in the future. So, what the KNAC needs is to increase its liberal arts educational programs and various cultural experiences in its educational curriculum to promote agricultural marketing with cultural knowledge and sensitivity.

• 산업융합연구
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• 제17권3호
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• pp.9-16
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• 2019

본 연구에서는 2018년도 9월 식중독의 원인과 주요 발생을 분석하고 HACCP 인증제와의 관련성을 분석하여 상관성을 보고자 하였다. 식중독 3개년 환자 수와 원인 물질 자료와 HACCP 인증업체와 식중독 발생빈도의 빅데이터 자료를 기반으로 가설 1: '학교급식을 매개로 살모넬라가 퍼졌을 것이다.', 가설 2: 'HACCP 인증업체가 증가할수록 식중독 발생 건수는 줄어들 것이다.'를 세우고 증명하기 위해 최근 3년 하절기 7월~9월까지의 월별 식중독 발생 건수의 차이를 검증하였고 2018년의 9월의 식중독 원인을 밝히기 위한 2018년 식중독 발생지와 원인 물질 간의 연관성 분석을 하였으며, 가설 2를 확인하기 위해 HACCP 인증업체 수와 식중독 발생 건수 간의 상관관계를 사용하였다. 연구결과 2018년도 9월의 식중독 원인은 살모넬라균이었으며 학교급식을 통해 제공된 외주 식품이 원인인 것을 알 수 있었다. 또한, HACCP 인증제의 확대가 식중독 감소에 유의하게 기여하지 않았음을 알 수 있었다. 따라서 살모넬라 예방법과 식중독을 감소시킬 수 있는 HACCP 인증이 되기 위한 해결 방안으로 관리운영방안의 보완을 제시하였다.

• 한국식품영양과학회지
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• 제44권12호
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• pp.1895-1904
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• 2015

본 연구에서는 건당 환자수가 높아 식품안전관리 우선순위가 높은 단체급식소의 식품, 조리도구 및 환경에서 식중독균을 분석하고 이들 미생물의 병원성 인자 및 항생제 내성을 확인하여 미생물 위험분석을 위한 기본정보를 제공하고자 하였다. 경기도 소재 총 8개(농촌 3, 도시 4 및 벽지 1) 학교급식소에서 식품 시료(n=66), 조리도구(n=44) 및 환경 시료(n=56) 등 총 179점의 시료를 채취하여 지표세균 및 식중독균을 분석하였다. 식품 시료에서 총균수는 평균 4.7 log CFU/g, 최대 8.1 log CFU/g으로 대장균군의 평균 오염도 3.1 log CFU/g, 최대 오염도 4.0 log CFU/g으로 높았다. 선반 및 개수대 등 환경 시료의 총균수는 평균 2.7 log CFU/g, 최대 4.1 log CFU/g으로 식품 시료보다 낮은 수준으로 분석되었으나 대장균의 경우 평균 4.0 log CFU/g, 최대 5.4 log CFU/g으로 식품 시료보다 오염 수준이 높아 환경으로 부터의 교차오염 가능성을 배제할 수 없었다. 병원성 미생물 중 Bacillus cereus의 정량분석 결과 식품(원료, 조리단계 및 조리식품 포함) 시료에서 평균 2.1 log CFU/g, 최대 4.1 log CFU/g으로 분석되었으나, 이 중 조리된 식품의 오염도는 10,000 CFU/g 이하로 식품공전의 기준 이하로 오염되어 있었다. Escherichia coli는 식품 중 조리 전 시료(n=14)에서만 검출율 35.7%로 분석되었으며 조리단계의 식품, 조리도구 및 환경 시료에서는 검출되지 않았다. Staphylococcus aureus의 경우 조리 전 식품 원료(n=14)의 21.4%에서 검출되었으며 환경 시료(냉장고 손잡이)에서 1건 양성으로 검출되었고, 조리단계의 식품, 조리도구 및 환경 시료에서는 검출되지 않았다. 그 외 Clostridium perfringens, Listeria monocytogenes, Salmonella spp., Vibrio parahaemolyticus는 분석된 모든 시료에서 모두 음성이었다. 분리된 B. cereus의 독소유전자(hblACD, nheABC, entFM, cytK enterotoxin gene)를 분석한 결과 구토 유발 독소인 ces는 모두 음성이었으나 분석된 86주 모두 적어도 1종 이상의 설사 유발 독소유전자가 검출되었으며 66.2%의 균주는 설사 유발 독소유전자를 모두 보유하고 있었다. 식품과 환경에서 분리한 S. aureus(n=16)의 장독소 생성 유전자를 분석한 결과 모두 1종 이상의 독소유전자가 검출되었다. 전형적인 장독소유전자 중에서는 sea만 검출되었으며, 독소충격증후군 toxin(tst) 유전자는 모든 분리주에서 검출되지 않았다. 집단 식중독 발생 시 초기 진료에 결정적 영향을 주는 항생제 내성 여부를 분석한 결과 E. coli(n=41)의 92.7%는 분석한 항생제 16종에 대해 내성을 보이지 않았고 cefazolin에 대한 내성률이 4.9%로 가장 높았으며, 1개 균주에서만 2개 항생제에 대해 다제내성을 보여 국내외 항생제 내성률보다 낮았다. S. aureus(n=16)는 시험한 19종 항생제 중 gentamicin에 대한 내성률이 62.5%로 가장 높았으며 일부 균주에서 2주 항생제에 대해 다제내성이 관찰되었다. 한편 단체 급식소 2개소의 조리도구와 환경 중 미생물 군집을 분석한 결과 특정균이 도구와 환경에서 중복 검출되어 도구와 환경 중 교차오염 가능성을 간접적으로 시사하였다. 이와 같이 본 연구에서 단체급식소 식품, 조리도구 및 환경 중 위생지표균과 병원성 미생물의 오염패턴을 분석하고 분리된 균주의 독성인자와 항생제 내성 정보를 분석하였다. 관련 정보는 단체급식소 미생물 위험분석과 이를 바탕으로 사전적, 정량적 안전관리 기술 개발에 활용 가능할 것으로 사료된다. 한편 식중독 유발의 다른 원인인 바이러스류와 기타 원인에 대한 연구는 진행되지 않아 추가 연구가 필요하다.

• 경영과학
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• 제32권2호
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• pp.79-89
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• 2015

The firms with OHSAS (Occupational Health and Safety Management System) registration should be audited every six months and they have to be reregistered every three years by a third party. In our article audit data were collected from the audit processes of fifty four firms that OHSAS 18001:2007 was implemented and audited by LRQA (Lloyd's Register Quality Assurance). Fifty four firms are largely classified as four categories: civil engineering and construction industry, metal and heavy industry, chemical industry, and other industries including electric, electronic, food, and services. Especially the minor conformity data are analyzed by statistical methods to find out the relationship between four categories of 54 firms and the average number of minor nonconformities. The most frequently appeared minor nonconformities among sub-requirements of OHSAS 18001:2007 are investigated and the reasons for minor nonconformities are specifically explained. The average number of minor nonconformities of firms before and after OHSAS 18001:2007 registration are compared to understand the maturity of OHSAS implementation. Some comments and suggestions are provided for OHSAS to be effectively implemented to the firms if revision is necessary.

• Journal of Preventive Medicine and Public Health
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• 제45권1호
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• pp.1-7
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• 2012

Using three Austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction analysis by pulsed field gel electrophoresis has become the gold standard for subtyping of food borne pathogens like listeria, salmonella, campylobacter and Bacillus cereus. Using a Salmonella Mbandaka outbreak from the year 2010 as example, it is shown how the comparison of patterns from human isolates, food isolates, animal isolates and feed isolates can allow to identify and confirm a source of disease. An epidemiological connection between the simultaneous occurrence of tuberculosis in cattle and deer with cases of human tuberculosis due to Mycobacterium caprae in 2010 was excluded using mycobacterial interspersed repetitive units variable-number tandem repeats subtyping. Also in 2010, multilocus sequence typing with nonselective housekeeping genes, the so-called sequence based typing protocol, was used to elucidate connections between an environmental source (a hospital drinking water system) and a case of legionellosis. During the last decades, molecular typing has evolved to become a routine tool in the daily work of public health laboratories. The challenge is now no longer to simply type microorganisms, but to type them in a way that allows for data exchange between public health laboratories all over the world.

• 농약과학회지
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• 제14권4호
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• pp.347-360
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• 2010

잔류농약 다성분 동시분석을 위하여 국내에 등록되어 사용되고 있는 농약 180종을 대상으로 서로 다른 흡착제를 사용하는 2종의 흡착크로마토그래피 체계를 확립하고자 하였다. 유럽과 미국, 우리나라의 흡착크로마토그래피 방법을 참고하여 Florisil과 silica-gel 크로마토그래피 용출용매체계를 정한 후 농약성분별 용출양상을 확인하였다. 칼럼정제실험 결과 Florisil 체계와 silica-gel 체계에서 각각 대상농약의 145와 137종이 70-120% 범위의 회수율을 나타내었다. 인접분획에 걸쳐 용출된 경우를 중복 계수하여 얻은 용출분획별 분포율은 용출분획 순서별로 Florisil 체계의 경우 12, 76, 81, 60 및 30성분 순이었고, silica-gel 체계의 경우는 22, 59, 102, 46 및 8성분 순이었다.

• 한국임상약학회지
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• 제33권1호
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• pp.22-34
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• 2023

Background: With the increasing use of narcotic analgesics, the Ministry of Food and Drug Safety has been operating the National Narcotics Information Management System (NIMS) since 2018 in Korea. Some studies are related to the narcotic analgesics use, but the evaluation studies of system, including NIMS users are insufficient. Objective: This study evaluated the NIMS enforcement process through in-depth interviews on the experience of using NIMS of doctors and pharmacists who prescribe or dispense opioid analgesics. Methods: Participants in this study were selected through purposeful sampling as three doctors and two pharmacists in the metropolitan area. The interviews were conducted from August 27 to October 19, 2022. Each interview was analyzed using a phenomenological method. Results: Six components of this study included "Doctors and pharmacists come across drug abusers", "They respond individually to drug abusers", "They use NIMS tailored to the needs of system managers", "They are concerned about the role of NIMS in practice settings", "They face challenges against drug abusers" and "They find a way to utilize the system to deal with drug abusers." The interviews confirmed that doctors and pharmacists used NIMS little in their work, and they only reported data. Therefore, it might be difficult for doctors and pharmacists to take measures for the safe use of narcotic analgesics. Conclusion: It is likely that adding data-feedback and medication-check function can reduce the abuse of narcotic analgesics. In the future, further studies on the evaluation of the empirical results of NIMS and review of systems in other countries will be required.