Adsorption Column Chromatography for Simultaneous Determination of Multi-pesticide Residues

잔류농약 다성분 동시분석을 위한 흡착 크로마토그래피의 적용

  • Kim, Chan-Sub (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Ihm, Yang-Bin (Agro-materials Management Division, Rural Development Administration) ;
  • Choi, Ju-Hyun (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Lee, Kyoung-Mi (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Lee, Young-Deuk (School of Life and Environmental Science, Daegu University)
  • 김찬섭 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 임양빈 (농촌진흥청 농자재관리과) ;
  • 최주현 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 이경미 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 이영득 (대구대학교 생명환경학부)
  • Received : 2010.11.23
  • Accepted : 2010.12.13
  • Published : 2010.12.31

Abstract

In order to develop the multi-residue purification method for 180 pesticides commonly used in Korea, many analytical methods on individual and multi- pesticide residues in the agricultural commodities and food product were examined. Through the modification of adsorption chromatographic methods used in Europe, the United States and Korea, the Florisil and silica-gel chromatographic systems were developed. Through these purification systems, elution profiles for all pesticides were examined. As the results, 145 pesticides were recovered in the range of 70-120% in Florisil clean-up system. The distribution of pesticides in the elution profile was 12 pesticides in the first fraction, 76 pesticides in the second fraction, 81 pesticides in the third fraction, 60 pesticides in the fourth fraction and 30 pesticides in the last fraction. And, in silica-gel system, 137 pesticides were recovered in the range of 70~120%. The distribution of pesticides in the elution profile was 22 pesticides in the first fraction, 59 pesticides in the second fraction, 102 pesticides in the third fraction, 46 pesticides in the fourth fraction and 8 pesticides in the last fraction.

잔류농약 다성분 동시분석을 위하여 국내에 등록되어 사용되고 있는 농약 180종을 대상으로 서로 다른 흡착제를 사용하는 2종의 흡착크로마토그래피 체계를 확립하고자 하였다. 유럽과 미국, 우리나라의 흡착크로마토그래피 방법을 참고하여 Florisil과 silica-gel 크로마토그래피 용출용매체계를 정한 후 농약성분별 용출양상을 확인하였다. 칼럼정제실험 결과 Florisil 체계와 silica-gel 체계에서 각각 대상농약의 145와 137종이 70-120% 범위의 회수율을 나타내었다. 인접분획에 걸쳐 용출된 경우를 중복 계수하여 얻은 용출분획별 분포율은 용출분획 순서별로 Florisil 체계의 경우 12, 76, 81, 60 및 30성분 순이었고, silica-gel 체계의 경우는 22, 59, 102, 46 및 8성분 순이었다.

Keywords

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