• 원예과학기술지
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• 제31권1호
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• pp.14-23
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• 2013

This study evaluated the influence of light quality and intensity during healing and acclimatization on the $CO_2$ exchange rate, growth, and morphogenesis of grafted pepper (Capsicum annuum L.) transplants, using a system for the continuous measurement of the $CO_2$ exchange rate. C. annuum L. 'Nokkwang' and 'Tantan' were used as scions and rootstocks, respectively. Before grafting, the transplants were grown for four weeks in a growth chamber with artificial light, where the temperature was set at $25/18^{\circ}C$ (light/dark period) and the light period was 14 hours $d^{-1}$. The grafted pepper transplants were then healed and acclimatized under different light quality conditions using fluorescent lamps (control) and red, blue, and red + blue light-emitting diodes (LEDs). All the transplants were irradiated for 12 hours per day, for six days, at a photosynthetic photon flux (PPF) of 50, 100, or 180 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The higher PPF levels increased the $CO_2$ exchange rate during the healing and acclimatization. A smaller increase in the $CO_2$ exchange rates was observed in the transplants under red LEDs. At a PPF of 180 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, the $CO_2$ exchange rate of the transplants irradiated with red LEDs was lowest and it was 37% lower than those irradiated with fluorescent lamps. The $CO_2$ exchange rates of transplants irradiated with blue LEDs was the highest and 20% higher than those irradiated under fluorescent lamps. The graft take was not affected by the light quality. The grafted pepper transplants irradiated with red LEDs had a lower SPAD value, leaf dry weight, and dry matter content. The transplants irradiated with blue LEDs had longer shoot length and heavier stem fresh weight than those irradiated with the other treatments. Leaves irradiated with the red LED had the smallest leaf area and showed leaf epinasty. In addition, the palisade and spongy cells of the pepper leaves were dysplastic and exhibited hyperplasia. Grafted pepper transplants treated with red + blue LEDs showed similar growth and morphology to those transplants irradiated with fluorescent lamps. These results suggest that high-quality grafted pepper transplants can be obtained by healing and acclimatization under a combination of blue and red lights at a high PPF level.

• 한국조경학회지
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• 제40권1호
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• pp.110-117
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• 2012

본 연구는 우리나라 자생종 굴거리나무에 대한 실내조명 하에서 광적응성을 조사해 보고 실내조경공간으로 도입할 때, 도입방안을 제시해 보고자 수행되었다. 공시수종인 굴거리나무는 제주도 농가에서 1년생을 구입한 후, 경남과학기술대학교 종합농장 유리온실에서 40일간 순화시킨 후 2010년 2월 15일에서 2010년 11월 15일까지 실험하였다. 첫째, 형광등의 경우, 100lux에서 500lux까지 식물이 고사하거나 잎이 지는 현상을 보여 생육상태가 좋지 못하였으며, 1,000lux부터 원활한 생육 및 생장을 보였다. 엽수나 수형, 체내 광합성률, 관상가치 등을 토대로 살펴볼 때, 형광등 1,000lux에서 가장 양호한 생장을 하여 최적광도인 것으로 조사되었다. 둘째, LED등의 경우,100lux에서 1,000lux까지 고사현상을 보여 생육상태가 불량하였으며, 광도가 높을수록 원활한 생장을 보였다. 2,000lux에서 가장 원활한 생육 및 생장을 하였다. 셋째, 삼파장의 경우,1,000lux까지 고사현상이 심하여 도입한 4개의 광원 중 생육상태가 가장 좋지 않아 전반적으로 삼파장은 굴거리나무 생육에 부적합한 것으로 파악되었다. 2,000lux에서 가장 원활한 생육 및 생장을 하였다. 넷째, 광섬유의 경우 100lux하에서도 식물 전체가 고사하는 현상은 보이지 않아 생육이 가능하여 도입광원 중 생육 및 생장상태가 가장 양호하였다. 대체적으로 광도가 높을수록 생장률이 높았으나,2,000lux보다 1,000lux하에서 엽수의 출엽률 및 수형이 흐트러지지 않아 관상가치가 더 좋았으며, 체내 광합성률도 높아 1,000lux를 조명해 주는 것이 가장 효과적인 것으로 파악되었다. 광적응성을 고려하여 굴거리나무를 실내조경공간으로 도입할 때, 광섬유, 형광등, LED등의 순으로 도입하는 것이 적합하며, 삼파장 등은 부적합한 것으로 파악되었다. 광원별 최적광도는 광섬유와 형광등을 도입할 경우 1,000lux, LED등을 도입할 경우 2,000lux를 조명해 주는 것이 효과적인 것으로 나타났다. 또한, 자생종 굴거리나무는 외래종인 대엽홍콩을 대체할 수 있는 수종으로 실내조경식물로 도입이 가능하며, 중심목으로 활용할 수 있을 것으로 기대되었다.

• 통합자연과학논문집
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• 제3권1호
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• pp.28-32
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• 2010

Thiophene-derivatized pentiptycenes were synthesized and characterized by NMR and UV-Vis spectroscopy. Aggregation behavior of thiophene-derivatized pentiptycenes was monitored by the measurement of fluorescence. Fluorescence intensities for the thiophene-derivatized pentiptycenes and thiophene-derivatized pentiptycenes aggregates were compared. There is no shift in the maximum of the emission wavelength. In the range of water fraction between 20% and 40%, the emission intensity of thiophene-derivatized pentiptycene aggregates remains almost identical. Fluorescence efficiency incresaed by about 5 times higher when the thiophene-derivatized pentiptycenes forms the aggregates in solution.

• 한국세라믹학회지
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• 제31권4호
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• pp.367-374
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• 1994

Inorganic-organic composite was made through sol-gel process. Possible range for hosting organic dye was investigated with various composition. The transmittance of the composite was the most important condition for a host was increased to 90% with decreasing HCl as a catalyst. Clear absorbance peak was obtained by decreasing concentration of organic dye, fluorescent intensity was increased and transmittance was decreased with rising gelling temperature.

• 한국컴퓨터산업학회논문지
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• 제10권3호
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• pp.99-104
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• 2009

본 논문에서는 형광등 on/off 제어 및 조도를 전력선 통신을 이용하여 원격으로 제어할 수 있는 시스템을 설계하고자 하였다. 각각의 조명기기를 전력선으로 구성되는 네트워크를 구현하였으며 원격 제어 및 조명기기의 on/off 및 조도를 제어하기 위하여 서버를 구성하고 서버에서 각각의 크라이언트를 제어할 수 있도록 하였다. 또한 기존 on/off 시스템을 디지털화하여 에러율을 감소시켰으며 회로를 단순화하는데 주안점을 두었다.

• 분석과학
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• 제36권5호
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• pp.250-257
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• 2023

The development of a spectrofluorimetric method for the determination of trimethoprim according to the reaction between O-phthalaldehyde (OPA) in highly alkaline media, and 2-mercaptoethanol (2ME) and this reaction gives fluorescent product measured at (458) nm when excited at (342) nm. The optimization of the analytical parameters that influence intensity was investigated. The intensity of fluorescence of the formed product was linearly related to the concentration of trimethoprim in the (100-1200) ng mL^-1 range. The limit of detection and limit of quantification were estimated to be (22.54) ng mL^-1 and (75.15) ng mL^-1 respectively. The utility of the proposed methods was successfully verified by analysis of trimethoprim in pure and real pharmaceutical preparations with high accuracy, the recovery percentages Re%, were found to be (100.5) % and (99.76) % for pure drug and pharmaceutical preparations respectively.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권1호
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• pp.19-24
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• 2024

For over five millennia, humans have benefited from the valuable byproducts of Bombyx mori silkworms nourished on mulberry leaves and a multitude of potential applications remains available due to the diverse array of silkworm varieties. In this work, we discuss the utilization of Daehwangjam (DHJ), golden silk (GS), and Juhwangjam (JHJ), distinctive colored silks found in Korea, as chemosensors. These novel silks emit fluorescence under external stimuli and show a diminishing fluorescence intensity when exposed to HCl vapor. The considerable surface-to-volume ratio of these cocoons allows for the identification of 5 ppm, 300 ppm, and 3000 ppm HCl vapors through decreased fluorescence intensity. The results show the suitability of natural DHJ, GS, and JHJ for applications in biosensing applications.

• Toxicological Research
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• 제34권1호
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• pp.1-6
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• 2018

The purpose of this study was to detect cell death in the liver of mice treated with thioacetamide (TAA) using fluorescence bioimaging and compare this outcome with that using conventional histopathological examination. At 6 weeks of age, 24 mice were randomly divided into three groups: group 1 (G1), control group; group 2 (G2), fluorescence probe control group; group 3 (G3), TAA-treated group. G3 mice were treated with TAA. Twenty-two hours after TAA treatment, G2 and G3 mice were treated with Annexin-Vivo 750. Fluorescence in vivo bioimaging was performed by fluorescence molecular tomography at two hours after Annexin-Vivo 750 treatment, and fluorescence ex vivo bioimaging of the liver was performed. Liver damage was validated by histopathological examination. In vivo bioimaging showed that the fluorescence intensity was increased in the right upper part of G3 mice compared with that in G2 mice, whereas G1 mice showed no signal. Additionally ex vivo bioimaging showed that the fluorescence intensity was significantly increased in the livers of G3 mice compared with those in G1 or G2 mice (p < 0.05). Histopathological examination of the liver showed no cell death in G1 and G2 mice. However, in G3 mice, there was destruction of hepatocytes and increased cell death. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed many cell death features in the liver of G3 mice, whereas no pathological findings were observed in the liver of G1 and G2 mice. Taken together, fluorescence bioimaging in this study showed the detection of cell death and made it possible to quantify the level of cell death in male mice. The outcome was correlated with conventional biomedical examination. As it was difficult to differentiate histological location by fluorescent bioimaging, it is necessary to develop specific fluorescent dyes for monitoring hepatic disease progression and to exploit new bioimaging techniques without dye-labeling.

• 식물조직배양학회지
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• 제21권6호
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• pp.363-367
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• 1994

식물 세포의 신호전달 기작에 있어 calmodulin (CaM)의 역할을 조사하기 위하여 외부기원의 CaM을 식물 세포 내로 주입하였다. 이를 위하여 bovine testis로부터 CaM을 분리, 정제하여 SDS-PAGE상에서 순수함을 확인하였고, 이를 세포 내로 주입한 후 실제 주입 여부를 확인하기 위해 정제된 CaM을 미리 fluorescein isothiocyanate (FITC)로 표식하여 사용하였다. 또한, FITC-CaM complex의 농도에 따른 fluorescent intensity를 측정하여 주입된 CaM의 농도를 결정하였다. 대두 현탁 배양 세포를 saponin (0.1 mg/mL)이 포함된 배지에서 15분간 배양하여 permeabilization시키고,FITC-CaM (1mg/mL)을 30분간 처리한 후 형광현미경으로 조사했을 때 세포질에서 강한 형광이 나타났으며, 이 결과로 외부의 calmodulin이 세포 내로 주입되었음을 확인할 수 있었다. 대두 현탁 배양 세포에 3가지의 세포벽 분해 효소를 처리하여 원형질체를 분리하였고, 이 원형질체를 FITC-CaM이 들어있는 완충용액에 넣고 electroporation 하였을때 capacitance와 field strength가 증가할수록 원형질체의 생존률은 감소하였으나, 세포 내의 fluorescent intensity는 증가하였다. 이것은 외부의 calmodulin이 electroporation에 의해 대두의 원형질체 내로 도입되었음을 의미하는 것이다.

• The Plant Pathology Journal
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• 제25권2호
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• pp.136-141
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• 2009

Marking biocontrol bacteria is an essential step to monitor bacterial behavior in natural environments before application in agricultural ecosystem. In this study, we presented the simple green fluorescent protein (GFP) reporter system driven by the promoter active in Bacillus species for tagging of the biocontrol bacteria. A constitutive promoter P43 from Bacillus subtilis was fused to an enhanced promoterless gfp gene by overlap extension PCR. The GFP expression was demonstrated by the high fluorescence intensity detected in B. subtilis and Escherichia coli transformed with the P43-gfp fusion construct, respectively. The GFP reporter system was further investigated in two bacterial biocontrol strains B. licheniformis and Pseudomonas fluorescens. When the reconstructed plasmid pWH34G was introduced into B. licheniformis, GFP level measured with the fluorescence intensity in B. licheniformis was almost equivalent to that in B. subtilis. However, GFP expression level was extremely low in other biocontrol bacteria P. fluorescens by transposon based stable insertion of the P43-gfp construct into the bacterial chromosome. This study provides information regarding to the efficient biomarker P43-gfp fusion construct for bio-control Bacillus species.