• Asian-Australasian Journal of Animal Sciences
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• 제16권11호
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• pp.1650-1655
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• 2003

Crossbred gilts (n=54) of about $26.38{\pm}0.85kg$ body weight and 25 weeks of age were randomly divided into 18 groups of 3 animals each. Three dietary treatments viz., $T_1$, $T_2$ and $T_3$ were formulated. The treatment $T_1$ containing maize grain (35%) and wheat bran (47%) along with soybean meal and fish meals served as control diet. Rice bran (RB) was selected as a single source of fat (13.02%) and fibre (17.12%), which was gradually increased in diets $T_2$ (41%) and $T_3$ (82%) replacing maize grain and wheat bran of $T_1$ at 50% and 100% in the diets $T_2$ and $T_3$, respectively. The fat and fibre levels of the diets were thus 3.46 and 5.24, 9.31and 9.69 and 11.61and 13.26% in $T_1$, $T_2$ and $T_3$, respectively. All the diets, however, contained almost similar concentration of CP (18.35${\pm}0.29%$). Each dietary treatment was offered to six replicated groups of 3 piglets in following completely randomized design and feeding was continued for 112 days during the growing phase. Growth, feed utilization, reproductive performance, nutrient utilization and different blood biochemical parameters were studied. Growth rate, feed intake and feed conversion were lower (p<0.01) in $T_3$ in comparison to $T_1$ or $T_2$. Digestibility of all the nutrients except EE was reduced significantly (p<0.01) in $T_3$. Serum glucose level decreased (p<0.01), whereas the urea and cholesterol concentration in the blood increased (p<0.01) in $T_3$. The duration of estrus of pigs fed diet containing 82% RB ($T_3$) was highly variable within the group, but all the groups showed statistically similar duration of estrous. Feed cost per unit gain was found to be comparable between control ($T_1$) and $T_2$ group. The results indicated that RB can be included up to 41% in swine diets replacing 50% of maize and wheat bran. Higher concentrations of ether extract and fibre beyond 9.31 and 9.69% in diet had detrimental effect on growth, nutrient utilization and reproductive performance.

• 한국어류학회지
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• 제32권2호
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• pp.103-109
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• 2020

붕장어(Conger myriaster)는 2018년 6월부터 2019년 5월까지 거제 구조라, 망치 인근해역에서 장구형통발을 이용하여 매달 1회 채집해 이용하였으며, 항문장(Preanus length)과 습중량을 각각 0.1 cm, 0.1 g까지 측정한 뒤 위를 적출하여 가능한 종 수준까지 분석하였다. 위내용물 분석결과는 각 먹이생물에 대한 출현빈도(%F), 개체수비(%N), 습중량비(%W)를 구하여 상대 중요도지수비(%IRI)로 수치화하여 주 먹이생물과 크기군에 따른 먹이조성 변화를 알아보았다. 붕장어의 가장 중요한 먹이생물은 어류(Pisces)였으며, 그중 멸치(Engraulis japonicus)가 가장 우점한 먹이생물로 확인되었다. 그 외, 새우류(Macrura), 패충류(Ostracoda), 갯지렁이류(Polychaeta), 게류(Brachyura) 등 다양한 먹이생물을 섭식하였지만 그 양은 많지 않았다. 과거 광양만에서 이루어진 연구에서는 붕장어의 주 먹이생물이 날개망둑(Favonigobius gymnauchen)으로 나타나 이번 연구결과와 다소 차이가 있었다. 성장에 따른 위내용물 조성의 변화를 살펴본 결과, 뚜렷한 먹이전환이 나타나지 않았다. 붕장어는 성장함에 따라 개체당 평균 먹이생물의 개체수(mN/ST)는 통계적으로 유의한 차이를 보이지 않았으나, 평균 먹이생물의 중량(mW/ST)은 붕장어의 항문장이 증가함에 따라 유의하게 증가하는 경향을 보였다.

• Toxicological Research
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• 제14권3호
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• pp.379-384
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• 1998

Endocrine disruptor is an exogenous substance that changes endocrine function and causes adverse effects at the level of the organism, its progeny, and/or (sub)populations of the organisms. Purported adverse effects are cancers, declines in reproductive health, developmental learning disabilities in humans, and declining populations, altered morphology, physiology or behavior in wildlife. In these days, expert groups on chemicals in IPCS, IFCS and OECD are intensively discussing the identification of endocrine disruptors and the proper management of those chemicals. In this study, we screened the endocrine disrupting effects of lead using fertilized eggs of Oryzias latipes. In brief, the eggs were exposed to lead with different concentrations at Ringer's solution, and the mortality, the incidence of deformation, the body movement and the hatching success were determined after incubation. The histological analysis of normal and deformed larvae was also carried out. Compared to control, the mortality and the heart rate of eggs and/or larvae increased, but the hatching success and the tail movement decreased. The morphological observation showed the asymmetrical deformation of larvae and the distortion of spinal cord. The absorption of the liquid in yolk sac was hindered. The adverse effects of lead in the ontogeny of fertilized eggs of Oryzias latipes seemed to be stronger in pH5.6 than in pH7.5 solution. In summary, lead showed adverse effects on the ontogeny of fish fertilized eggs plays critical role in regulating biological systems and controlling developmental processes as an endocrine disruptor.

• 한국어류학회지
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• 제1권1_2호
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• pp.1-8
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• 1989

1989년(年) 8월(月), 경남(慶南) 진해만(鎭海灣), 자란만(紫蘭灣)에서 채포(採捕)된 성숙한 친어(親魚)를 사용한 수조내(水槽內)에서의 쑤기미의 난발생(卵發生) 및 부화자어(孵化仔魚)의 형태(形態)에 대한 관찰(觀察) 결과(結果)를 요약하면 다음과 같다. 1. 수정난(受精卵)은 직경이 1.2~1.3 mm인 분리부성난(分離浮性卵)으로, 유구(油球)가 없다. 수온(水溫) $24.9{\sim}26.5^{\circ}C$에서 E+26~29시간만에 부화하였다. 2. 부화직후(孵化直後) 자어(仔魚)는 전장(全長)이 2.47~3.10 mm였으며, 입과 항문(肛門)이 열리지 않으며, 몸 전체에 황색소포(黃色素胞)가 산재하여 있고, 난황(卵黃)을 위로하여 떠있다. 근절수(筋節數)는 13+16~17=29~30개였다. 3. 부화(孵化) 후(後) 2일째 전장(全長) 3.61~4.16 mm의 자어(仔魚)는 가슴지느러미가 부채형으로 크게 발달하여, 그 위에 3~4개의 커다란 흑색반점(黑色斑點)이 나타났다. 4. 부화(孵化) 3일째 전장(全長) 3.97~4.29 mm의 자어(仔魚)는 난황(卵黃)을 완전히 흡수하였으며, 가슴지느러미 줄기가 4~5개 발달하였다. 5. 부화(孵化) 6일째 자어(仔魚)는 전장(全長)이 4.07~5.46 mm 범위이며, 가슴지느러미에 11개의 줄기가 발달하였고, 흑색반점(黑色斑點)은 줄기끝부분에서 8~9개로 증가하였다.

• 한국식품영양과학회지
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• 제39권11호
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• pp.1619-1626
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• 2010

본 연구는 한국과 일본의 일부지역 여대생들을 대상으로 식습관과 식품기호도를 조사 비교함으로써 우리나라 여대생들의 식생활 실태를 대외적 차원에서 파악하고자 하였으며 결과를 요약하면 다음과 같다. 한국여대생은 일본여대생에 비해 체중에는 차이가 없었으나 신장이 더 커서 BMI는 더 적었다. 한국여대생은 일본여대생보다 밥식과 된장국 섭취횟수는 많았으나 아침 결식과 외식 횟수는 2배 이상 높았고 패스트푸드, 인스턴트식품, 냉동식품의 섭취 횟수가 높아 식습관이 바람직하지 못하였으며 활동 정도도 낮았다. 양국 대학생들은 모두 생선류보다는 육류와 우유 및 유제품에 대한 기호도가 높아 식생활의 서구화 경향을 보였고 채소류보다는 과일류에 대한 기호도가 높아 단맛에 대한 기호를 나타내었다. 한국여대생은 일본여대생과 비교하여 육류에 대한 기호도에는 차이가 없었으나 곡류, 생선류, 콩 및 콩제품, 채소류, 김을 제외한 해조류에 대한 기호도가 낮았으며 반면에 우유와 요구르트에 대한 기호도는 높았다. 결론적으로 한국여대생은 일본여대생에 비해 아침 결식, 외식, 패스트푸드, 인스턴트식품, 냉동식품의 섭취 횟수가 높아 바람직하지 못한 식습관을 지니고 있었고 식품기호도에서도 섭취를 권장하는 생선류, 콩 및 콩제품, 채소류, 해조류에 대한 기호도가 낮아 전반적으로 바람직하지 못한 식생활실태를 보였으며 더욱이 활동 정도까지 낮아 일본여대생보다 장차 식생활과 관련한 질병 유발 가능성을 더 많이 내포하고 있었다. 따라서 여대생 자신의 건강과 미래의 가정과 사회를 이끌 그들의 위치를 고려하여 우리나라 여대생들이 이러한 자신들의 실태를 자각하고 식습관을 바르게 교정하여 바람직한 식생활을 할 수 있도록 이와 관련한 교육이 절실히 요구된다.

• 한국환경보건학회지
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• 제37권6호
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• pp.440-449
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• 2011

Objectives: This study was designed to determine the levels of polybrominated diphenyl ethers (PBDEs) in breast milk and to evaluate the relations with factors affecting these levels. Methods: The congener levels of PBDE in 22 samples of breast milk were analyzed using a high resolution gas chromatograph with a high resolution mass detector. In accordance with our standard operating procedures, the recoveries of internal standards had to range between 68% and 118%. Since the distribution of PBDE concentrations is close to log-normal, the data were logarithmically transformed before analysis. Test subjects were healthy primipara and multipara mothers with a mean age of 32 (SD = 2.7) in 2006. Results: Seven PBDE congeners (BDE-28, 47, 99, 100, 153, 154, and 183) were detected and identified in all of the pooled breast milk samples, indicating widespread contamination from PBDEs in the environment in Korea. Residue levels of total PBDEs (sum PBDEs from tri- to hepta-BDE) ranged from 0.84-13.1 ng/g lipid with median and geometric mean levels of 2.6 ng/g lipid and 2.74 ng/g lipid, respectively. PBDE congeners 47, 99 and 153 markedly predominated and accounted for about 75% of the amount of the PBDE congeners analyzed. BDE-47 was the dominant congener in most samples, whereas BDE-153 was predominant in a few (n = 7/22). BDE-47 was highly correlated with total PBDEs (r = 0.987, p < 0.01). In analyses of the differences of the means of log transformed breast milk PBDE levels for groups of potential covariates, only breast milk BDE-47 and BDE-99 levels were significantly associated with fish (p < 0.05) and meat consumption (p < 0.01). However, we did not find significant correlations between PBDE levels and maternal age, body mass index (BMI), parity, job presence and smoking status. Conclusions: Our findings are mainly limited due to the small sampling size and low doses of PBDEs exposure. Background and human exposure data of PBDEs is lacking, and longitudinal investigations into the environment and biota are encouraged to determine the health impact on future populations in Korea.

• Nutritional Sciences
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• 제4권1호
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• pp.3-12
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• 2001

Long chain polyunsaturated fatty acids (LCPUFA) are important components of brain phospholipds and play important role (s) in brain function. In rats, the maximum brain growth occurs during the period of lactation even though it happens during the third trimester of gestation in human. Since milk contained docosahexaenoic acid (DHA) even through the maternal diet had no DHA and/or a very small amount of its precursor, $\alpha$-linolenic acid ($\alpha$-LnA), an emphasis was given to maternal adipose tissue as a reservoir of this fatty acid. We, therefore, investigated the mesenteric and subcutaneous adipose tissues for their fatty acid composition in dams reared with different fat diets. Diets containing various amounts of $\omega$6 and $\omega$3 fatty acids were given to adult female rats (200-250g) throughout the pregnancy and lactation periods. Diets were composed of 10% (wt/wt) corn oil (CO), soybean oil (SO), perilla seed oil (PO) containing about 60% $\alpha$-LnA, or fish oil (FO) rich in eicosapentaenoic acid (EPA) and DHA. The fatty acid ompositions of mesenteric and subcutaneous fat were measured and evaluated at Day-2 and Day-15 after parturition. In general, major characteristics of dietary fatty acid composition was reflected on the fatty acid composition of adipose tissues. Dietary fatty acid composition was reflected more on mesenteric fat as compared to subcutaneous fat. Mesenteric fat was found to contain less arachidonic acid (AA) and mesenteric fats of CO, SO and PO groups contained less DHA than did the subcutaneous fat. The P/M/S ratios of adipose tissues were similar between experimental groups while dietary P/M/S ratios differed significantly. It was noticeable that a small proportion of DHA was found in the adipose tissues of animals of CO, SO and PO groups (Day-2) and in SO and PO groups (Day-15), the groups which do not contain DHA in their diets. The percentage of DHA in mesenteric fat o CO, SO and PO groups decreased as lactation continues, while the proportion of DHA in FO group increased. Adipose tissues of FO group had higher DHA/EPA ratio as compared to the diet. Considering the fact that the body contains a large amount of adipose tissues, our present finding suggests that the adipose tissue can serve as a reservoir of DHA for pregnant and lactating rats.

• 한국식품영양학회지
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• 제13권4호
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• pp.334-341
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• 2000

해조류에서 생합성되는 3급 함황화합물인 DMPT를 창자파래로부터 추출하기 위하여 시료의 상태, 용매 의 극성, 초음파 처리, boiling 및 autoclaving 처리에 의한 추출조건을 검토하였다. 용매추출, 초음파 처리후 용매추출, 열수추출 및 가압 열수추출법을 이용하여 창자파래로부터 DMPT를 추출한 결과, 초음파 처리후 가압 열수처리한 경우가 가장 효과적이었고, 다음으로는 초음파 처리후 열수 처리하는 방법이었다. 창자파래로부터 DMPT를 추출 해내는 최적조건으로 분말시료에 물과 acetone (2:1, v/v)의 혼합용매를 15배량 가하여 초음파 처리 후 121$^{\circ}C$에서 60분간 autoclaving하는 방법으로서, 이 때 추출된 DMPT 함량은 시료 g당 311.200ng이었다. 창자파래에서 추출한 DMPT 수용액을 조제하여 온도별로 저장시킬 때 DMPT 분해에 의한 DMS의 발생량은 저장온도에 비례하여 증가되었지만 저온에서는 매우 낮은 분해율을 나타내었다. DMPT 1mg을 저장하였을 때의 잔존율은 6$0^{\circ}C$ 저장의 경우78.5%로 고온에서는 다소 불안정하였으나 $0^{\circ}C$에서는 DMPT의 잔존율이 99.8%로 매우 높게 나타났다. 창자파래에서 추출한 DMPT 수용액을 3$0^{\circ}C$에서 방치하였을 때 pH가 5 이하의 산성영역에서는 DM-PT의 분해가 거의 일어나지 않았으나 pH가 올라감에 따라서 분해가 서서히 일어나기 시작하여 pH 9.5에 이르러서는 DMS의 양이 급속하게 증가하였다.

• 수산해양기술연구
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• 제22권4호
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• pp.49-55
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• 1986

The authors carried out an experiment to determine the horizontal opening of the mid water trawl gear, which is the same as was used in the foreward experiment concerned to the attitude and opening efficiency of otter board. The trawl net .used in the experiment was designed to have a square sectional four-seamed body with two three-sepenlted wings. Each wing tip was rigged with a net pendant of 70 m long and the fore ends of the pendants were directly jointed to the otter pendant without inserting hand rope, and a front weight of 200 kg weigh was rigged with in front of the lowest wing tip. To determine the opening between otter boards ani between wing tips, two 50 KHz fish finders were set sideways on the otter board and on the port middle wing tip so as to detect the distance froa the finders to the starboard otter board and to the wing tip respectively, and the informations were transmitted to the indicators onboard the ship through water-tight cables. The results obtained can be summarized as follows: 1. The openings between otter borads were 43-45 m in case of the warp 100 m, 53-55 m in the war;> 150 m and 54-59 m in the warp 200 m. It was increased in linear according to the increase of towin; speed in the range of 1. 1-1. 8 mlscc. 2. The determined values of the openings between otter boards were greater than the estimated values. which were calculated by the opening between warps at the gallows of the ship and the length of tt~ warp, assuming that the warps from the top rollers to the otter boards were straight. The rates of the determined values to the estimated values were 1. 23-11. 0 in case of the warp 100 m, 1. 23-1. 12 in the warp 150 m and 1. 23-1. 15 in the warp 200 m respectively. The rates were almost the same at the slow towing speed of 1. 3 mlscc or so, then the higher the speed, the less the rate, and the longer the warp, the greater the rate. 3. The rates of the openings between otter boards to the total length of net pendant and the net (excluded cod end) showed 43-43% in case of the warp 150-200 m. It was a little smaller than th~ standard. which is 50%. in the common bottom trawl. 4. The determined openings between wing tips were 18-19 m in case of the warp 100m, 21-22 m in the warp 150 rn, They showed that the higher the speed, the greater the openings even though its range was no more than 1 m or so. 5. The rates of openings between otter boards to th~ openings betwee:l wing tips were almost invariably 38%. 6. The rates of openigs b2tween otter boards to the lenth of h~ad rope were 60-65% in cas': of th~ warp lOa-150m. It were much larg2r than th~ standard. which is 50%. in the common botto:n trawl.

• Environmental Analysis Health and Toxicology
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• 제30권
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• pp.4.1-4.11
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• 2015

Objectives: This survey was designed to conduct the first nationwide dietary exposure assessment on hazardous substances including the intakes of functional food and herbal medicine. In this paper, we introduced the survey design and the results of the dietary exposure status and internal exposure levels of lead (Pb), cadmium (Cd), and mercury (Hg). Methods: We selected 4867 subjects of all ages throughout Korea. We conducted a food survey, dietary survey, biomonitoring, and health survey. Results: Pb and Cd were the highest (median value) in the seaweed ($94.2{\mu}g/kg$ for Pb; $594{\mu}g/kg$ for Cd), and Hg was the highest in the fish ($46.4{\mu}g/kg$). The dietary exposure level (median value) of Pb was $0.14{\mu}g/kg$ body weight (bw)/d, $0.18{\mu}g/kg$ bw/d for Cd, and $0.07{\mu}g/kg$ bw/d for Hg. Those with a blood Pb level of less than $5.00{\mu}g/dL$ (US Centers for Disease Control and Prevention, reference value for those 1 to 5 years of age) were 99.0% of all the subjects. Those with a blood Cd level with less than $0.30{\mu}g/L$ (German Federal Environmental Agency, reference value for non-smoking children) were 24.5%. For those with a blood Hg level with less than $5.00{\mu}g/L$ (human biomonitoring I, references value for children and adults, German Federal Environmental Agency) was 81.0 % of all the subjects. Conclusions: The main dietary exposure of heavy metals occurs through food consumed in a large quantity and high frequency. The blood Hg level and dietary exposure level of Hg were both higher than those in the European Union.