• 동의생리병리학회지
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• 제26권2호
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• pp.189-198
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• 2012

Our former study indicated efficacy of apoptotic cell death on animal study by using Egg white combined Chalcanthite (EC). Clinically, bamboo salt is using because of safety. Hence we investigated a toxicity study for determining safety by adding bamboo salt in former materiel. We had two studies: toxicity of EC and of Bamboo salt with egg white combined Chalcanthite (BC). Both were studied in 1-week single and 5-week repeated oral dose toxicity tests on male Imprinting Control Region mice. In EC, doses used in 1 week single oral dose toxicity tests were 0, 0.05, 0.5, 5 and 50 mg/kg/day and 0, 0.01, 0.05, 0.25 and 0.5 mg/kg/day. In BC, doses used by 0, 0.08, 8.3, 83.3 and 166.6 mg/kg/day in single oral dose toxicity and 0, 4.2, 8.3, 41.7 and 83.3 mg/kg/day in repeated oral dose toxicity tests. Their blood and urine were assayed and organ morphology were examined. Mann-Whitney U test and ANOVA tests were used by analysing methods. First, significant increased left renal weight in all groups of EC and BC. Second, increased ALT score was found in EC-S2 and increased relative liver weight was found in EC-S3. In addition, increased relative weight and urine bilirubin and urobilinogen were found in EC-R2 and EC-R3. There was no significant toxic change in BC. The Mixture of EC had a possibility of hepatotoxicity in the short and long term. Processed BC appears to be safe and non-toxic in these studies and a no-observed adverse effect level (NOAEL) was established at 83.3 mg/kg/day in mice. Relatively, The BC were safer than The EC.

• 한국산업보건학회지
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• 제10권1호
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• pp.1-17
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• 2000

Carcinogen-DNA adduct analysis has potential for biomonitoring the earliest effects of exposure to many chemical carcinogens. They are the covalent reaction products of electrophiles and nucleophilic sites on DNA and the initial damage to DNA induced by many carcinogens. So many researchers begin to use them as biomarker for monitoring the earliest exposure of carcinogens and develop the effective analytical techniques about them. Randerath, Gupta and coworkers(1981, 1982) has also developed a $^{32}P$-postlabelling method as one among them. A major project for biomonitoring workers with carcinogen-DNA adducts is to develop non-invasive samples instead of tissues of target organs such as baldder and lung. This study use the exfoliated urothelial cells in urine for examine benzidine-DNA adducts. The content of exfoliated urothelial cells is not enough to significantly measure DNA content with spectrophotometer, and require the another way. So firstly washing the collected cells with PBS and 70% ethanol and centrifuge them for removing the crystals in urine, which block the isolation of DNA adducts. And then, measure the total nucleotide after $^{32}P$-postlabelling for calculating RAL. $[{\gamma}-^{32}P]ATP$ using for $^{32}P$-postlabelling, can synthesize with $[^{32}P]H_3PO_4$, and reagent and enzyme mixture (RM, EM), which is very economic in case of requiring a lot of them. Chromatography was composed of two steps. First step was to separate adduct ones from unadducted nucleotide, and secondary step was separate each adduct, which were performed with 4 kinds of solvents and different directions on TLC. With this procedure, we measure the DNA adducts in exfoliated urothelial cells of workers who were employed in benzidine and benzidine-dye company. RAL of adducts were $89.0{\times}10^7$ and $57.0{\times}10^7$ in them. In conclusion, we can significantly measure the DNA adduct in exfoliated urothelial cells by using the above $^{32}P$-postlabelling procedures, and use them to be biomonitoring workers who exposed carcinogens.

• 한국산업보건학회지
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• 제25권1호
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• pp.104-114
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• 2015

Objectives: The purpose of this study is to investigate the atmospheric concentration of VOCs and the urinary concentration of t,t-MA, HA, MA in the industrial complex of Yeosu, South Jeolla Province. Methods: In order to study seasonal patterns of air concentration of VOCs, measurements were taken at five sampling sites around Yeosu from June 2013 to June 2014. Urinary metabolite excretionsfrom 671 subjects, exposure and comparison area were analyzed. Results: The average concentration of VOCs in the air was 1.53ppb for benzene, 0.73ppb for toluene, 0.22ppb for ethylbenzene, 0.52ppb for xylene and 0.12ppb for styrene. The concentration of benzene was somewhat higher than the year-average standard ($5{\mu}g/m^3$, about 1.5ppb) of the domestic air-environment criteria newly established in 2010.The metabolic concentration of VOCs in the urine of the entire sample was analyzed at $47.76{\mu}g/g\;cr.$, 213.07mg/g cr., and $290.09{\mu}g/g\;cr.$ for t,t-MA, HA, and MA, respectively. Compared with the average values for Korea as presented in the first basic survey of national environmental conservation ( $49.8{\mu}g/g\;cr.$ for t,t-MA, 0.17g/g cr. for HA, and 0.26mg/g cr. for MA), the metabolic concentrations of HA and MA in urine were higher than the average values. Conclusions: The concentration of VOCs in the air and urinary metabolites of the exposed and control areas showed that the concentrations of all substances were higher in the exposed area than in the control area.

• Childhood Kidney Diseases
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• 제5권1호
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• pp.15-21
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• 2001

목 적 : 요로감염은 어린이에서 중요한 신요로계 질환으로 빠르고 정확한 진단과 치료로 신장 손상의 위험을 줄이고 불필요한 검사와 치료를 피할 수 있다. 저자들은 우리나라 어린이 요로감염 치료의 개선을 도모하는 첫걸음으로 설문 조사를 통해 요로감염의 진단, 치료, 영상 검사와 예방에 관한 진료 행태를 알아보았다. 대상 및 방법 : 전국의 소아신장전문의와 비뇨기과 전문의를 대상으로 설문조사를 통하여 요로감염의 진단, 치료, 영상검사, 예방의 진료형태를 조사하였다. 결 과 : 응답자들은 대부분 신생아에서는 소변 채취용 백을, 이후에는 중간소변 채취를 이용하여 소변 배양 검사를 하였고, 소변 배양검사가 음성인 경우에는 농뇨, nitrite 양성, 세균뇨 등의 소견을 보일 때 요로감염이라고 판단하였다. 요로감염 후에는 80$\%$가 예방적 항생제를 사용하였다. 방광 요관 역류가 있는 환아에서는 1-2세 이상의 환아가 고도의 방광 요관 역류를 보이거나 예방 요법 중에도 요로감염이 발생할 때 수술로 치료하는 경우가 많았으나 그 기준에는 차이를 보였다. 대부분이 요로감염 진단시에 신장 초음파 검사를 시행하였고 과반수에서 각각 다양한 시기에 배뇨 방광 요도 조영 검사와 DMSA scintigraphy를 시행하였다. 포경 수술은 대개 권하지 않았으며 반수에서 방광 요관 역류 환아의 형제에 대한 선별 검사를 시행하였다. 결 론 : 이 보고에서 밝혀진 다양한 치료 행태로 보아, 어린이 요로감염 치료의 개선과 발전을 위해 체계적인 진료 방침의 고안이 필요할 것으로 보인다.

• Clinical and Experimental Pediatrics
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• 제52권9호
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• pp.1053-1058
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• 2009

거대세포바이러스(CMV)는 신생아에서 가장 흔한 선천성 감염의 원인 중 하나이며, 모든 정상 출산아 중 약 0.3-2.4%에서 감염되어 있다. 혈청에서 거대세포바이러스 양성인 모체 중 40-96%에서 모유를 통한 바이러스의 배출이 증명되었으며, 거대세포바이러스에 감염된 모유를 통한 감염은 전체 영아 거대세포바이러스 감염의 약 1/3을 차지한다. 본 증례에서는 극소 저체중 출생아에서 모유를 통하여 발생한 거대세포바이러스 감염을 기술하였다. 환아는 생후 7일 경부터 모유 수유를 하였으며, 심한 혈소판감소증, 빈혈, 그리고 패혈증과 유사한 임상 양상에 대하여 반복되는 혈소판 수혈, 적혈구 수혈, 면역 글로불린 요법을 시행받았다. 거대세포바이러스 감염은 생후 2개월 경 혈청 CMV IgM 양성 및 소변 CMV 배양 검사 양성 소견을 통하여 진단하였다. 출생 당시 혈청 CMV IgM 및 소변 CMV 배양 검사가 음성이었으므로 선천성 감염은 배제할 수 있었다. 환아의 혈청과 모유의 핵산 배열 순서 분석을 통하여 동일한 바이러스에 의한 감염임을 증명하였다. 저자들은 국내에서 최초로 nucleotide sequencing 방법을 이용, 모유 수유를 통한 거대세포바이러스 감염이 발생한 극소 저체중 출생아의 예를 보고하는 바이다.

• Clinical and Experimental Pediatrics
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• 제53권2호
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• pp.222-227
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• 2010

목 적 : 본 연구는 소아 요로 감염증에서 치료 전 발열 기간과 치료 후 발열 기간 등의 임상변수와 혈액 및 소변 등의 검사 결과가 신 실질 병변 및 방광요관 역류 등을 예측하는 데 있어서 인자로 작용할 수 있는지를 평가해 보고자 하였다. 방 법 : 2005년 7월부터 2008년 7월까지 첫 번째 열성 요로 감염으로 본원 소아과에 입원한 1개월부터 만 17세까지의 환아 180명을 대상으로 하였다. 환아의 혈액 검사 소견 중 C-반응 단백, 백혈구수와 소변 검사 중 배양 검사 결과, 소변 질산염 및 치료전 발열기간, 치료 후 발열 기간 등을 변수로 하여 신장 초음파 배뇨 방광, 요도 조영술, 신 스캔 등의 영상 검사 결과와 비교하여 연관성을 평가하였다. 결 과 : C-반응 단백 수치가 높고 백혈구 증가증이 있으며 입원 치료 후 긴 발열 기간을 가진 경우 신 실질 병변이 유의하게 증가하였고 3단계 이상의 방광 요관 역류 발생도 높았다. 신 스캔 검사상 이상 소견을 보인 경우는 신 스캔 검사상 정상 소견을 가진 군과 비교하였을 때 3단계 이상의 방광 요관 역류가 더 증가되어 있었다. 결 론 : C-반응 단백 수치, 백혈구 증가증, 입원 치료 후 긴 발열기간 등의 임상 변수는 신 실질 병변과 3단계 이상의 방광 요관 역류의 예측 인자가 될 수 있을 것으로 사료된다.

• 대한간호
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• 제33권3호
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• pp.56-69
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• 1994

This study was done to develop self - directed learning modules related to asepsis and elimination including urine and stool for Fundamentals in Nursing practice education contents and to measure the effectiveness of those modules. The subjects of this study were 96 sophomore students in the nursing college. Self-directed learning modules were developed by the researcher on the basis of the Lippincott Learning System of Kruger (1986) and Modules for Basic Nursing Care of Ellis (1992). Videotape was editted by using videotape made by the Lippincott Company and Film strip made by the Trainex Company with Korean dubbing. Self-directed learning was done for one week with the asepsis module and two weeks with the elimination modules after confirming the requiered level of knowledge acquisition through pre-test. For measuring proficiency in self-directed learning, a written test for cognitive domain, a sufficiency test for psychomotor domain, and a confidnece examination for affective domain were given. The data were analyzed using descritive statistics, and Pearson correlation coefficient using a SPSS-PC program. The results are summarized as follows: 1. Sufficiency test and confidence examination grades showed high levels in both asepsis and elimination. 2. Written test grades showed a high level in asepsis and elimination of urine but showed a medium level in eliminationin of stool. 3. Grades of sufficiency and confidence in asepsis and elimination practice were statistically significant with a moderate positive correlation (r=0.4- 0.5, p<0.001). 4. Grades of sufficiency and written tests in asepsis and elimination practice also were statistically significant with a moderate positive correlation (r=0.5-0.7, p<0.001). 5. Students showed relatively high contentment with the self-directed learning modules themselves but revealed relatively low contentment with video program and the self-directed learning process. In conclusion, this study disclosed that proficiency levels in cognitive, affective, and psychomotor domains were high when asepsis and elimination modules were applied. Also students showed high satisfaction with the modules themselves, but didn't show high contentment with the video programs. In considering low contentment with the self-directed learning process, it is estimated the students had experienced some difficulties about using self-directed learning modules because this was their first exposure to the self-directed learning module and they were already accustomed to the demonstration-practice method.

• 한국산업보건학회지
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• 제9권1호
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• pp.23-40
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• 1999

The analysis of thiodiglycolic acid in urine has been used as an index of biological exposure to vinyl chloride. Unfortunately thiodiglycolic acid has a strong hydrophilic character, because it has two carboxylic groups, so that it can only be extracted with organic solvent with a great difficulty. Underivatized thiodiglycolic acid tends to tail because of non-specific interaction with the inert support. Therefore, esterification is the obvious first choice for derivatization of thiodiglycolic acid, particularly for gas chromatography. In this study, the focus of interest is to compare two method of esterifications (methylation and silylation). Methylation is to make the methyl ester of thiodiglycolic acid by reaction with diazomethane. Silylation is to make the trimethylsilyl ester of thiodiglycolic acid by reaction with N-trimethylsily-ldiethylamine. The results and conclusions are as the following: 1. The detection limit (sensitivity) of methylated thiodiglycolic acid was $5.00{\mu}g/m{\ell}$ and silylated thiodiglycolic acid was $3.07{\mu}g/m{\ell}$ by gas chromatography with flame ionization detector. 2. The optimal liquid-liquid extraction of thiodiglycolic acid was as following: To each of the tubes, $15m{\ell}$ of urine, concentrated sulfuric acid (pH 1 - 2) and 5 gsodium sulfate were added. The samples was extracted three times with $5m{\ell}$ ethylacetate each time. 3. The methylated thiodiglycolic acid was more stable than silylated thiodiglycolic acid in extractional solvent which contained humidity. 4. The precision (pooled coefficient of variation for 4 days) of the analysis was 0.07324 in methylated thiodiglycolic acid with external standard calibration, and 0.07033 in methylated thiodiglycolic acid with internal standard calibration. 5. The precision (pooled coefficient of variation for 4 days) of the analysis was 0.10914 in silylated thiodiglycolic acid with external standard calibration, and 0.13602 in silylated thiodiglycolic acid with internal standard calibration. From the above results, the analysis of methylated thiodiglycolic acid was more sensitive (limit of detection) than silylated thiodiglycolic acid by gas chromatography. However, the methylated thiodiglycolic acid was stable in the humidity and was separated sharply on chromatogram. Also, analysis of methylated thiodiglycolic acid was more precise (pooled coefficient of variation for 4 days) than silylated thiodiglycolic acid. In conclusion, it is established that the analysis of methylated thiodiglycolic acid is appropriate for biological monitoring of exposure to vinyl chloride.

• 한국식품조리과학회지
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• 제26권6호
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• pp.804-810
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• 2010

본 연구는 건강한 남성들을 대상으로 신선한 브로콜리와 조리된 브로콜리를 섭취시킨 후, glucosinolates의 가수분해물질인 ITC가 mercapturic acid pathway를 거쳐 소변을 통해 배설되는 양을 측정하였다. 브로콜리를 섭취시키기 전 8일간은 glcusinolate가 함유되지 않는 식사(control period)를 섭취하도록 하였다. 그 이후에 조리되지 않은 신선한 브로콜리를 각각 3일간 250 g과 500 g씩 섭취시키면서 실험기간 동안 배설되는 소변을 수집하였다. 1차 실험이 종료된 후에 다시 8일 동안의 세척기간을 거친 후, 전자레인지에 가열한 브로콜리를 250 g과 500 g씩 각각 3일간 섭취시키면서 실험기간 내내 소변을 수집하였다. 수집된 소변으로부터 SF mercapturic acid의 양을 측정하여 HPLC로 분석하였다. 섭취한 브로콜리의 양과 소변으로 배설되는 mercapturic acid의 양은 직선의 상관관계를 보였다. 대조군과 비교할 때, 신선한 브로콜리를 섭취했을 때, 소변으로 배설되는 mercapturic acid의 양이 3일과 6일째 각각 3.8배와 1.9배 증가하였다. 브로콜리를 전자레인지에 익혀서 섭취시켰을 때는 소변으로 배설되는 mercapturic acid의 양이 신선한 경우와 비교하여 감소하였다. 이는 가열조리를 통해 glucosinolate의 양이 감소하였음을 의미한다. 식이로 섭취한 glucosinolate로부터 전환된 ITC가 소변으로 배설되는 생체마커를 측정하고 십자화과 채소를 통해 섭취하는 SF의 양과 소변을 통해 배설되는 양과의 상관관계가 있음을 확인하였다. 본 실험결과는 십자화과 채소 섭취의 양을 예측하는 유용한 생체마커로 활용할 수 있을 것으로 사료된다.

• Journal of Pharmaceutical Investigation
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• 제9권2호
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• pp.11-21
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• 1979

It has been reported from our department that a few agents, such as $K_2S_2O_5,\;NaHSO_3,$ nicotinamide have a marked stabilizing effect in vitro on metoclopramide which is relatively unstable compound. In order to study the effect of these stabilizers on the action of metoclopramide in vitro, the fate of this compound combined with $K_2S_2O_5,\;NaHSO_3$ and nicotinamide, respectively, was studied and furthermore, the change of the biological activity of metoclopramide due to these stabilizers was studied by using the isolated stomach strip of rat. The blood concentration of metoclopramide was measured by using Bakke's method at the various time after intravenous injection of the mixed metoclopramide solution with the stabilizers. In order to study the excretion of the drug, rabbits were anesthesized and catheterized into bladder for withdrawal of urine. After intravenous injection of the mixed metoclopramide solution, urine was collected for 5 hours and the conjugated forms of metoclopramide as well as the free form were determined by using Arita's method. In the biological study of the metoclopramide combined with stabilizers, the contractability of the isolated rat stomach strip was observed by using polygraph recorder. The results were following: 1. When metoclopramide was administered with nicotinamide as stabilizer, the blood concentration of the unchanged from and the rate of the clearance of this compound were very similar to that of metoclopramide alone. On the other hand, other stabilizers, $K_2S_2O_5\;and\;NaHSO_3$, brought about 40% decrease in blood concentration of the unchanged form at 15 min after intravenous injection however, the rate of clearance of metoclopramide with $K_2S_2O_5\;or\;NaHSO_3$ was very slow. 2. In the case of urinary excretion, the excretory pattern of the metabolites of metoclopramide with $NaHSO_3$ or nicotinamide was very similar to that of metoclopramide alone. But metodopramide plus $K_2S_2O_5$ group showed the maked depression of excretion for first 1 hour. 3. In composition of metabolites, when metoclopramide was administered with $K_2S_2O_5$ or $NaHSO_3$, the sulfonate conjugation was predominant. But the glucuronic acid conjugation was predominant in metoclopramide plus nicotinamide gronp. 4. In the experiments on the biological activity of the metoclopramide, this compound exhibited the marked contracting effect in isolatd rat stomach strip. Specially, the meetoclopramide combined with $K_2S_2O_5$ showed the strong contraction of the isolated strip, suggesting the potenciating effect of $K_2S_2O_5$ on the action of metoclopramide in the isolated strip.