• Molecules and Cells
• /
• v.42 no.10
• /
• pp.729-738
• /
• 2019

Autophagy is an important process for protein recycling. Oligomerization of p62/SQSTM1 is an essential step in this process and is achieved in two steps. Phox and Bem1p (PB1) domains can oligomerize through both basic and acidic surfaces in each molecule. The ZZ-type zinc finger (ZZ) domain binds to target proteins and promotes higher-oligomerization of p62. This mechanism is an important step in routing target proteins to the autophagosome. Here, we determined the crystal structure of the PB1 homo-dimer and modeled the p62 PB1 oligomers. These oligomer models were represented by a cylindrical helix and were compared with the previously determined electron microscopic map of a PB1 oligomer. To accurately compare, we mathematically calculated the lead length and radius of the helical oligomers. Our PB1 oligomer model fits the electron microscopy map and is both bendable and stretchable as a flexible helical filament.

• Animal cells and systems
• /
• v.6 no.3
• /
• pp.277-282
• /
• 2002

Topoisomearse II is an essential enzyme in all organisms with several independent roles in DNA metabolism. Recently, it has been demonstrated that the C-terminal region of topoisomerases II is associated with hetero-logous protein-protein interactions in human and yeast. In this study, we identified that RTP1, a rat homologue of EIA binding protein BS69, is another topoisomerae II interacting protein by yeast two-hybrid screening. RTP1 has an E1A-binding domain and a MYND motif, which are known to be required for transcriptional regulation by binding to other proteins and interaction with the leucine zipper motif of topoisomerase II. The physical interaction between RTP1 and topoisomerase ll$\alpha$ was examined by GST pull-down assay in vitro. The expression level of RTP1 peaks in S phase as that of topoisomerase ll$\alpha$. These results suggest that the interaction between topoisomerase ll$\alpha$ and RTP1 might play an important role in regulating the transcription of genes involved in DNA metabolism in higher eukaryotes.

• Journal of The Korean Society of Clinical Toxicology
• /
• v.20 no.1
• /
• pp.22-24
• /
• 2022

Elapid snakes have neurotoxic venom which causes diverse neuroparalytic manifestations, including fatal respiratory failure. In South Korea, since elapid snakebites are very rare, the cobra antivenom, which is effective against neurotoxicity, was only introduced recently. Most physicians in South Korea have little experience in the treatment of patients who have been bitten by elapid snakes. A 19-year-old man was brought to the emergency department with sudden diplopia, 1 hour after a snakebite on the left 2nd finger. The patient presented with drowsiness and complained of mild dizziness and binocular diplopia. After 1 hour, he had sudden onset of dyspnea and dysphagia and appeared to be agitated. He was immediately intubated and received mechanical ventilation as he was unable to breathe on his own. A total of 2.5 mg of neostigmine diluted with normal saline was slowly infused, and 1 vial of cobra antivenom was infused for an hour, 5 times every 2 hours, for a total of 5 vials. He slowly recovered self-breathing; on the 3rd day of hospitalization, he showed tolerable breathing and was extubated. He was discharged without any neurological deficits or other complications.

• Investigative Magnetic Resonance Imaging
• /
• v.21 no.2
• /
• pp.91-96
• /
• 2017

Purpose: To describe technical methods for functional magnetic resonance imaging (fMRI) study with arterial spin labeling (ASL) compared to blood oxygenation level-dependent (BOLD) technique and discuss the potential of ASL for research and clinical practice. Materials and Methods: Task-based (n = 1) and resting-state fMRI (rs-fMRI) (n = 20) were performed using ASL and BOLD techniques. Results of both techniques were compared. Results: For task-based fMRI with finger-tapping, the primary motor cortex of the contralateral frontal lobe and the ipsilateral cerebellum were activated by both BOLD and ASL fMRI. For rs-fMRI of sensorimotor network, functional connectivity showed similar results between BOLD and ASL. Conclusion: ASL technique has potential application in clinical and research fields because all brain perfusion imaging, CBF measurement, and rs-fMRI study can be performed in a single acquisition.

• The Journal of Korean Medicine
• /
• v.29 no.1
• /
• pp.192-199
• /
• 2008

Objective : Silent infarct is more common in healthy elderly people and seems associated with risk of future stroke. However, the prevalence and risk factors of silent infarct are unclear. We investigated the prevalence and risk factors of silent infarct. Methods : 56 first-ever cerebral infarct patients were enrolled in this study. CT images were made of all 56 patients. We divided them into two groups according to the presence of silent infarcts and comparedage, sex, education period, WHR, hypertension, diabetes mellitus, hyperlipidemia, smoking, numbness and weakness of thumb and index finger, neck stiffness, facial spasm, and blepherospasm. Result : Silent infarcts were found in 24 patients(43%). Most infarcts(48%) were located in basal ganglia. Age, sex, education period, WHR, numbness and weakness of thumb and index finger, neck stiffness, facial spasm, and blepherospasm were similar between the two groups. Diabetes mellitus, hyperlipidemia, and smoking were higher in the silent infarct group. Hypertension was higher in the non-silent infarct group. Conclusion : The prevalence of silent infarct in first-ever cerebral infarction patients was 43% and diabetes mellitus, hyperlipidemia, and smoking were higher in silent infarct patients.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.37 no.4
• /
• pp.519-525
• /
• 2010

Ectopic eruption means the eruption of the tooth in an abnormal position due to multiple factors, which found most frequently in maxillary fist permanent molars, mandibular lateral incisors and maxillary permanent canines. Ectopic eruption of the maxillary first permanent molar occurs when the molar erupts with a more mesial angulation than normal, and locks itself in an atypical resorption on the distobuccal root of the second primary molar. The maxillary first permanent molar plays important roles for mastication and occlusion, so ectopically erupted maxillary first permanent molars should be relocated into proper position. Treatment options are separation by insertion of the brass wire or elastic rings, preparation of distal aspect of the maxillary second primary molar, using fixed or removable appliance with finger spring, and placement of space maintainer or space regainer after extraction of the maxillary second primary molar. We report three cases treated of ectopically erupted maxillary first permanent molar by re-setting of stainless steel crowns, placement of brass wire and using active plate. We could find out distal movement of maxillary first permanent molars into proper position and normal occlusion.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.24 no.1
• /
• pp.81-86
• /
• 2015

This paper presents a disposable passive suction pump that uses the restoring force of an elastomeric chamber for liquid transportation in a microfluidic system. The proposed suction pump can be operated by finger pressure without any peripheral equipment. To adjust the generated suction pressure, five different displacements of the suction chamber ceiling, two different chamber shapes, and five different elastic moduli of the elastomer were considered. For a cylindrical chamber with a 5 mm height and 5 mm radius, the generated suction pressure and flow rate increased almost linearly up to about 31 kPa and $160.8{\mu}L/min$, respectively, depending on the chamber deformation. A maximum suction pressure of $42.9{\pm}0.7kPa$ was obtained for a hemispherical chamber with a 2.1 mm height and 5 mm radius.

• Journal of Life Science
• /
• v.22 no.8
• /
• pp.1018-1023
• /
• 2012

Snail is a zinc finger transcription factor that induces epithelial-to-mesenchymal transition (EMT), which promotes tumor invasion and metastasis by repressing E-cadherin expression. In addition, Snail restricts the cellular apoptotic response to apoptotic stimuli or survival factor withdrawal; however, its molecular mechanism remains largely unknown. In this study, we have investigated the mechanism underlying Snail-mediated chemoresistance to 5-fluorouracil (5-FU), one of the most widely used anti-cancer drugs. When Snail was overexpressed by doxycycline (DOX) in MCF-7 #5 cells, it inhibited 5-FU-induced apoptotic cell death and switched the cell death mode to necrosis. Snail expression, either by DOX treatment in MCF-7 #5 cells or by the transfection of Snail expression vectors pCR3.1-Snail-Flg, phosphorylation-resistant pCR3.1-S104, and 107A Snail-Flg in MCF-7 cells specifically induced PTEN down-regulation/inactivation and Akt/PKB activation, without affecting ERK1/2 activity. In addition, Snail prominently suppressed 5-FU-induced increases in p53 levels. These findings demonstrate that Snail switches 5-FU-induced apoptosis to necrosis through the activation of Akt/PKB and the down-regulation of p53 levels.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.16 no.1
• /
• pp.17-26
• /
• 2006

In order to obtain basic reference information for the establishment of effective industrial safety programmes in migrant workers who live in Seoul and Kyungin Area, accident causes were investigated among 210 injured workers. The data were analyzed according to the USA Standards Institute and International Labour Organization method. The results obtained were as follows: 1. The major distribution of national were mostly from china(82.9%), ages are mostly between 30 and 40 years old(32%), and males are more than females, and mostly married. 2. The parts of body most frequently injured were hand and finger, foot and toe, which were 55.3% of total 210 cases. 3. According to the accident type, caught in, under or between were most frequently observed, 40.2% of the total number of injuries. 4. The most responsible source of injuries were power machine, others, moving machine and tool, building construction. 5. According to the unsafe acts, carelessness and unsafety information were most frequently observed, 66.7% of the total number of injuries. 6. There were significantly difference(p<0.05), between type of accident and source of injury, and between working period and unsafe act.

• Journal of Acupuncture Research
• /
• v.32 no.1
• /
• pp.141-147
• /
• 2015

Objectives : The purpose of this study was to report a case of patient with trigger thumb which improved by Meridian Tendino-musculature Acupuncture therapy, Moxubustion and Bee-Venom pharmacoacupuncture. Methods : In this study, we have conducted Meridian Tendi-musculature Acupuncture therapy, Bee-Venom Pharmacopuncture and Moxibustion treatment, on the basis of anatomical structure on the patient who was diagnosed of trigger thumb with pain, edema and motional restriction of the thumb. Furthermore, Electroacupuncture had been implemented with acupuncture therapy, and also passive exercise therapy had been conducted before and after the treatment to enhance the effect. The patient was assessed for trigger thumb-related symptoms by Visual Analogue Scale (VAS) score and Quinnell's Classification of triggering. Results : Quinnell's triggering grade & VAS score had decreased considerably after 18 sessions of treatment. Conclusions : It can be deducted that symptoms of Trigger thumb could be improved by Meridian Tendino-musculature Acupuncture therapy, Moxibustion and Bee Venom Pharmacopuncture treatment.