• 농업과학연구
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• 제47권4호
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• pp.791-802
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• 2020

Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the ��-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.

• 한국토양비료학회지
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• 제45권6호
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• pp.928-935
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• 2012

World Agriculture faces daunting challenges in feeding the growing population today. Reduction in arable land extent due to numerous reasons threatens achievement of food and nutritional security. Under this back ground, agricultural use of acidic soils, which account for approximately 40 % of the world arable lands is of utmost important. However, due to aluminum (Al) toxicity and low available phosphorous (P) content, crop production in acidic soils is restricted. Citrus, in this context, gains worldwide recognition as a crop adapted to harsh environments. The present paper reviewed Al toxicity and possible toxicity alleviation tactics in citrus. As reported for many other crops, inhibition of root elongation, photosynthesis and growth is experienced in citrus also due to Al toxicity. Focusing at toxicity alleviation, interaction between boron (B) and Al as well as phosphorus and Al has been discussed intensively. Al toxicity in citrus could be alleviated by P through increasing immobilization of Al in roots and P level in shoots rather than through increasing organic acid secretion, which has been widely reported in other crops. Boron-induced changes in Al speciation and/or sub-cellular compartmentation has also been suggested in amelioration of root inhibition in citrus. Despite the species-dependent manner of response to Al toxicity, many commercially important citrus species can be grown successfully in acidic soils, provided toxicity alleviation Agro-biological tactics such as addition of phosphorous fertilizers are used properly.

• Journal of Ecology and Environment
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• 제41권11호
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• pp.318-327
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• 2017

Background: A typical sewage treatment plant is designed for organic and nutrient removal from municipal sewage water and not targeted to eliminate micropollutants such as pesticides, pharmaceuticals, and nano-sized metals which become a big concern for sustainable human and ecological system and are mainly discharged from sewage treatment plant. Therefore, despite contaminant removal by wastewater treatment processes, there are still remaining environmental risks by untreated pollutants in STP (sewage treatment plant) effluents. This study performed aquatic toxicity tests of raw wastewater and treated effluents in two sewage treatment plants to evaluate toxicity reduction by wastewater treatment process and analyze concentration of contaminants to reveal potential toxic factors in STP effluents. Methods: Water samples were collected from each treatment steps of two STPs, and acute and chronic toxicity tests were conducted following USEPA (United States Environmental Protection Agency) and OECD (Organization for Economic Cooperation and Development) guidelines. Endpoints were immobility for mortality and reproduction effect for estrogenicity. Results: Acute $EC_{50}s$ (median effective concentration) of influents for Seungki (SK) and Jungnang (JN) STPs are $54.13{\pm}32.64%$ and $30.38{\pm}24.96%$, respectively, and reduced to $96.49{\pm}7.84%$ and 100%. Acute toxicity reduction was clearly correlated with SS (suspended solids) concentration because of filter feeding characteristics of test organisms. Chronic toxicity tests revealed that lethal effect was reduced and low concentration of influents showed higher number of neonates. However, toxicity reduction was not related to nutrient removal. Fecundity effect positively increased in treated wastewater compared to that in raw wastewater, and no significant differences were observed compared to the control group in JN final effluent implying potential effects of estrogenic compounds in the STP effluents. Conclusions: Conventional wastewater treatment process reduced some organics and nutritional compounds from wastewater, and it results in toxicity reduction in lethal effect and positive reproductive effect but not showing correlation. Unknown estrogenic compounds could be a reason causing the increase of brood size. This study suggests that pharmaceutical residues and nanoparticles in STP effluents are one of the major micropollutants and underline as one of estrogenic effect factors.

• Nutrition Research and Practice
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• 제2권4호
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• pp.195-199
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• 2008

Alcoholism has been associated with folate deficiency in humans and laboratory animals. Previous study showed that ethanol feeding reduces the dehydrogenase and hydrolase activity of 10-formyltetrahydrofolate dehydrogenase (FDH) in rat liver. Hepatic ethanol metabolism generates acetaldehyde and acetate. The mechanisms by which ethanol and its metabolites produce toxicity within the liver cells are unknown. We purified FDH from rat liver and investigated the effect of ethanol, acetaldehyde and acetate on the enzyme in vitro. Hepatic FDH activity was not reduced by ethanol or acetate directly. However, acetaldehyde was observed to reduce the dehydrogenase activity of FDH in a dose- and time-dependent manner with an apparent $IC_{50}$ of 4 mM, while the hydrolase activity of FDH was not affected by acetaldehyde in vitro. These results suggest that the inhibition of hepatic FDH dehydrogenase activity induced by acetadehyde may play a role in ethanol toxicity.

• 환경생물
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• 제21권4호
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• pp.328-336
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• 2003

Biological and ecological characteristics of Diporeia spp. are described including size, growth, life cycle, energy storage, temperature effect, bioturbation, feeding depth and sediment ingestion of Diporeia. Bioaccumulation and toxicity of organic contaminants and trace metals were reviewed in addition to an examination of the relationships among various condition indexes (i.e. wet weight, dry weight and body length) of Diporeia.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.151-159
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• 2004

A detailed evaluation of relative toxicity of fluoride to die 5th instar larvae of PM and NB$_4$D$_2$ races of silkworm and the effects of lethal, sublethal and prevailing levels of fluoride in groundwater on the growth, economic characters and fecundity of the silkworm, Bombyx mori (Lepidoptera: Bombycidae) were studied. The feeding of mulberry, Morus alba leaves treated with lethal and sublethal doses of fluoride to PM and NB$_4$D$_2$ races of silkworms from the beginning of the 5th instar to the end of the feeding period resulted in significant reduction in growth, single cocoon weight, single shell weight, silk index, average filament length, and fecundity when compared to controls. These manges were more pronounced on exposure to lethal dose than sublethal dose and in general, the changes induced by fluoride were more striking in NB$_4$D$_2$ than PM, indicating the greater resistance of PM to higher fluoride levels. Groundwater quality in sericulturally important villages of Karnataka and Andhra Pradesh States has been studied with special reference to the presence of fluoride. On exposure to dose of prevailing levels in the waters of sericulturally important areas, die changes observed on growth, economic characters and fecundity were slightly lower when compared to controls and the decrease was found to be insignificant (P$\geq$0.05). It was concluded that, though minute dose (4.0 ppm) of fluoride did not have any toxic impact, it is toxic at higher concentrations to silkworms.

• 한국해양생명과학회지
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• 제1권2호
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• pp.121-125
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• 2016

본 연구는 유류오염에 의한 요각류 Tigriopus japonicus의 먹이섭식능력의 감소가 먹이회피에 의한 것인지 오염된 해수의 독성에 의한 것인지를 알아보기 위해, 유류(WAF 또는 CEWAF)에 오염된 먹이에 대한 T. japonicus의 먹이 섭식력을 조사하였다. WAF가 없는 배양수 조건에서는 모든 실험구에서 섭식량의 차이를 보이지 않았지만 WAF가 배양수에 있는 조건에서는 WAF의 농도가 증가할수록 섭식량이 줄어드는 경향을 보여 WAF 100% 농도에서는 대조구에 비해 유의하게 감소하였다. T. japonicus가 CEWAF에 오염된 환경에서 먹이의 종류에 따른 먹이선택성여부를 조사한 결과, 오염된 먹이를 공급했을 때, 배양수에 CEWAF가 존재하는 상태에서, 먹이로 Tetraselmis suecica를 공급한 실험구가 다른 실험구에 비해 유의적으로 낮은 부착규조류 섭식률을 보였다. 결과적으로, 유류오염에 의한 T. japonicus의 먹이 섭식력의 감소는 오염된 먹이회피에 의한 섭식력의 감소가 아니라 해수에 존재하는 유류독성에 의한 것으로 판단된다.

• 한국식품위생안전성학회지
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• 제23권3호
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• pp.177-181
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• 2008

희나리 고추에서 분리한 곰팡이를 현미에 배양하여 실험동물(rat)에 투여하였을 때 Colletotrichum, Diaporthe, Diaporthopsis, Botryosphereia, Aspergillus, Fusarium의 경우 독성을 나타내지 않았으나 Alternaria alternata로 동정된 곰팡이는 독성을 나타내었다. A. alternata를 배양한 현미를 투여한 실험동물은 사료 섭취량이 상대적으로 낮았으며, 체중 감소를 보였고 2주안에 치사하였으며, 위, 소장, 간의 확대가 관찰되었다. Alternaria속 곰팡이는 17종이 분리되었으며 이들 중 8종은 고추즙과 현미에 배양하였을 때 모두 상당량의 tenuazonic acid를 생산하였고, 현미 배지에서만 alternariol과 alternariol monomethyl ether 독소가 추가로 소량 검출되었다. 건조 과정에서 곰팡이가 번식된 고추는 유해한 대사물의 존재 가능성이 있으므로 식품 안전을 위해 곰팡이 번식을 최대한 억제할 필요가 있다.

• 한국패류학회지
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• 제28권4호
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• pp.349-359
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• 2012

The purpose of this study is to compare the clearance rate (CR) and intake rate (IR) of juvenile purple clam, Saxidomus purpuratus when feeding on different unialgal diet of red tide dinoflagellates (RTDs), and to know what is the most important cell characteristic of RTDs to cause the differences in feeding parameters. Experiments were performed to measure the CR and IR of juvenile S. purpuratus as a function of algal concentration when food was either the standard food, Isochrysis galbana or one of 9 RTDs. Patterns of CR with increasing algal concentration were similar among different RTDs. The highest $C_{max}$ was observed when S. purpuratus was feeding on A. affine, while the lowest on C. polykrikoides. The patterns of IR with increasing algal concentration were also similar among different RTDs. However, there were great differences in the maximum value of IR ($I_{max}$) among different RTDs. The highest $I_{max}$ was observed when S. purpuratus was feeding on A. carterae, while the lowest on G. catenatum. Some RTDs similar in size showed different $C_{max}$. Other RTDs different in size showed similar $I_{max}$. Life form of each RTD affected significantly the $I_{max}$, which was higher for single-celled RTDs than chain-forming RTDs. There were no significant differences in feeding parameters between toxic and nontoxic RTDs. Moreover, a toxic dinoflagellate, A. carterae recorded the highest $I_{max}$ among RTDs. The most important characteristic of RTD as a factor affecting the feeding rate of S. purpuratus was life form, not size or toxicity of RTD species.

• 한국축산식품학회지
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• 제36권4호
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• pp.494-498
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• 2016

Cold plasma has been developed to reduce microbial contamination and to improve safety of food and medical products. In addition, the technology can be used in the manufacture of sausages without addition of nitrite. To be applied in food industry commercially, the new technology should be safe and efficient. However, toxicological test of plasma-treated food is limited. Therefore, the purpose of this study was to determine the mutagenicity and immune toxicity of the meat products cured with plasma-treated water (PTW) as a nitrite source. Emulsion sausages were prepared with no nitrite (control), sodium nitrite (SCS), and PTW (SCP). For a mutagenicity test, the Ames test was performed with the sausage samples. For immune toxicity test, 8-wk-old female Balb/c mice were given free access to the sausages in order to evaluate the tumor necrosis factor (TNF)-α level. As a result, no mutagenicity was detected in the sausages by the Ames test. The serum TNF-α values were less than 10 pg/mL in mice after feeding control and treated samples for 32 d, indicating that no inflammatory response was occurred by feeding the sausages made by PTW. Therefore, the present study opens the possibility of using plasma-treated water as a nitrite source without any toxicity.